##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2695280
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97205744857677	33.0	31.0	34.0	30.0	34.0
2	32.086369505209106	34.0	31.0	34.0	30.0	34.0
3	32.20836833278917	34.0	31.0	34.0	30.0	34.0
4	35.7531410465703	37.0	35.0	37.0	35.0	37.0
5	35.563472440711166	37.0	35.0	37.0	33.0	37.0
6	35.59950468967973	37.0	35.0	37.0	33.0	37.0
7	35.53038719539343	37.0	35.0	37.0	33.0	37.0
8	35.48676538244635	37.0	35.0	37.0	33.0	37.0
9	37.11429721587368	39.0	37.0	39.0	34.0	39.0
10-11	37.02753461606957	39.0	37.0	39.0	33.5	39.0
12-13	36.922738082870794	39.0	37.0	39.0	33.0	39.0
14-15	38.242645105517795	40.0	38.0	41.0	33.0	41.0
16-17	38.15013801905553	40.0	38.0	41.0	33.0	41.0
18-19	38.045791346353624	40.0	38.0	41.0	33.0	41.0
20-21	37.93547367991452	40.0	38.0	41.0	33.0	41.0
22-23	37.90759364518714	40.0	38.0	41.0	33.0	41.0
24-25	37.97166880620937	40.0	38.0	41.0	33.0	41.0
26-27	37.87505824997774	40.0	38.0	41.0	33.0	41.0
28-29	37.748512028434895	40.0	38.0	41.0	33.0	41.0
30-31	37.61102501409872	40.0	38.0	41.0	32.5	41.0
32-33	37.46149787777151	40.0	37.0	41.0	32.0	41.0
34-35	37.33688151138286	40.0	37.0	41.0	32.0	41.0
36-37	37.25884008340506	40.0	37.0	41.0	31.5	41.0
38-39	37.23891970407527	40.0	37.0	41.0	32.0	41.0
40-41	37.08708891098513	40.0	37.0	41.0	31.0	41.0
42-43	36.870071013030184	40.0	36.5	41.0	31.0	41.0
44-45	36.687213944376836	39.5	36.0	41.0	30.5	41.0
46-47	36.44243418123534	39.0	36.0	41.0	30.0	41.0
48-49	36.14284582677867	39.0	35.0	40.0	29.5	41.0
50-51	35.887160703155146	39.0	35.0	40.0	29.0	41.0
52-53	35.596774361105346	38.5	35.0	40.0	28.0	41.0
54-55	35.29548636876317	38.0	34.5	40.0	28.0	41.0
56-57	34.94556558131252	38.0	34.0	40.0	27.0	41.0
58-59	34.581809867620436	37.5	34.0	40.0	26.0	41.0
60-61	34.175254890029976	37.0	33.5	39.5	26.0	41.0
62-63	33.75138668338725	36.0	33.0	39.0	25.5	40.5
64-65	33.2595372280431	36.0	32.5	39.0	24.5	40.0
66-67	32.75588992609302	35.0	32.0	38.5	22.5	40.0
68-69	32.27345860170372	35.0	31.0	38.0	22.0	40.0
70-71	31.743437602030216	35.0	31.0	37.0	20.5	39.0
72-73	31.20798024695022	34.0	30.5	36.5	19.5	39.0
74-75	30.700847036300495	34.0	30.0	36.0	18.0	38.5
76-77	29.35496479030008	32.5	28.5	34.5	17.0	36.0
78-79	30.30131025348016	34.0	30.0	35.0	18.0	37.0
80-81	30.3480115238491	34.0	30.0	35.0	18.0	37.0
82-83	30.03115984239114	34.0	30.0	35.0	15.0	36.0
84-85	29.57096776587219	34.0	29.5	35.0	8.5	36.0
86-87	29.082562479593957	34.0	29.0	35.0	4.5	35.0
88-89	28.567711146893828	33.5	29.0	35.0	2.0	35.0
90-91	28.140847147606188	33.0	27.5	35.0	2.0	35.0
92-93	27.745850709388264	33.0	27.0	35.0	2.0	35.0
94-95	27.293640363895403	33.0	26.5	34.0	2.0	35.0
96-97	26.582271044195778	32.5	25.0	34.0	2.0	35.0
98-99	25.627623104093082	32.0	23.5	34.0	2.0	35.0
100-101	22.024131073580484	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15717.0
3	8349.0
4	4818.0
5	3679.0
6	3296.0
7	3276.0
8	3754.0
9	4477.0
10	5366.0
11	6291.0
12	7611.0
13	8836.0
14	9745.0
15	10858.0
16	12511.0
17	14423.0
18	15033.0
19	13510.0
20	13409.0
21	14250.0
22	15229.0
23	16925.0
24	19752.0
25	23344.0
26	28646.0
27	34225.0
28	41849.0
29	51419.0
30	62969.0
31	79568.0
32	103275.0
33	138790.0
34	198177.0
35	306009.0
36	482835.0
37	621766.0
38	282463.0
39	8830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.746443258315935	17.808366014726072	13.634998375390195	24.810192351567796
2	16.239759876524886	19.084547802083645	38.343177703244194	26.33251461814728
3	19.82427368186709	22.166004971986197	28.474305220585507	29.535416125561202
4	14.091659510929993	15.220019876427573	34.68655645904079	36.00176415360165
5	15.558024906641831	35.589413285892114	33.13340246691884	15.71915934054722
6	35.149324075734405	33.400159119622245	16.440665569981263	15.009851234662088
7	31.096498474893764	30.5191301974748	19.37959829277213	19.00477303485931
8	27.798830435420463	33.861995836513515	18.963288151519713	19.375885576546313
9	26.800821611456215	16.320782008922453	19.083452387274455	37.79494399234688
10-11	26.39685839977524	25.029018912847818	26.41076668051305	22.163356006863893
12-13	26.545149408033236	22.064310361827722	26.892461831192865	24.49807839894618
14-15	23.717101477254587	23.78825398829037	24.352591971071206	28.142052563383835
16-17	24.13837898185862	26.911102846695336	25.036874388055892	23.913643783390157
18-19	24.186065242461012	26.184356403525015	26.43747167554924	23.192106678464732
20-21	24.622114562606313	25.390271777644873	26.328386281613238	23.65922737813558
22-23	24.516881808595258	25.03859340718077	26.229449173067348	24.21507561115662
24-25	24.221943488268213	25.263707001993634	26.325568310139218	24.188781199598935
26-27	24.178672062956384	25.450376314018936	26.283440290556502	24.087511332468175
28-29	24.24401832462433	25.54557210269108	26.245261168945373	23.96514840373922
30-31	24.444738383347985	25.41335158310526	26.038093368806358	24.1038166647404
32-33	24.24146796983515	25.447720670314368	26.017193646228087	24.2936177136224
34-35	24.375767142053558	25.428616272390904	26.14674286511509	24.04887372044045
36-37	24.365800176290044	25.458494195063025	26.05359833364904	24.122107294997885
38-39	24.389194464570487	25.504407062060675	26.05837675765287	24.04802171571597
40-41	24.516329425176625	25.391452722486235	26.01779192238411	24.074425929953026
42-43	24.457637048423546	25.362153490078832	25.904729784130808	24.275479677366814
44-45	24.36283991194503	25.411317457092686	25.942337538486942	24.283505092475345
46-47	24.380295023916094	25.432684796311477	25.973018920229833	24.214001259542595
48-49	24.41351918173276	25.449932890139255	25.84895597092246	24.28759195720552
50-51	24.39176064843244	25.688681188001254	25.69567532368749	24.22388283987882
52-53	24.464706199907948	25.630322954424408	25.67532846509504	24.229642380572606
54-55	24.454697325129793	25.39806747422016	25.75236695913349	24.39486824151656
56-57	24.404369782258513	25.541153569236176	25.762139721797134	24.292336926708177
58-59	24.412583083557816	25.522812603712413	25.7773897705726	24.287214542157166
60-61	24.575226305414077	25.462526821416766	25.741250643287312	24.220996229881848
62-63	24.39680602148479	25.57119816125632	25.744860584882428	24.287135232376464
64-65	24.457784698977886	25.40120017765568	25.751038643820767	24.389976479545663
66-67	24.415529174918863	25.55947297782819	25.659594273989768	24.36540357326318
68-69	24.409899725646994	25.533262053526677	25.681873025419417	24.374965195406908
70-71	24.37714302041177	25.39675736811258	25.76131087124187	24.464788740233782
72-73	24.55587613744922	25.387859142237655	25.56687819133419	24.48938652897893
74-75	24.3599190460741	25.595646870422488	25.51785938338707	24.52657470011634
76-77	24.452110057327108	25.403530135063317	25.632127742240897	24.512232065368682
78-79	24.359914009156018	25.406470105557844	25.73478359342222	24.498832291863913
80-81	24.369978070944587	25.559254007568743	25.57679057130413	24.49397735018254
82-83	24.493298128397072	25.494890812183375	25.541522693285252	24.47028836613431
84-85	24.47088349333881	25.470066534891995	25.524579910244043	24.534470061525155
86-87	24.444761420277082	25.400072099620058	25.680818585135036	24.474347894967828
88-89	24.378797717760683	25.27508801585647	25.631310258551594	24.714804007831255
90-91	24.379285088148663	25.427312997912637	25.636346445307556	24.557055468631145
92-93	24.42189360028597	25.515634494439706	25.69434571722239	24.368126188051942
94-95	24.43243156999731	25.410828618090463	25.552587380334806	24.60415243157742
96-97	24.41935147541884	25.40305061011831	25.600944368979196	24.576653545483655
98-99	24.444159141756742	25.38059924819856	25.596202736667085	24.579038873377616
100-101	24.84445765367042	24.982542301062008	25.683911385388075	24.4890886598795
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	322.0
1	266.0
2	387.5
3	531.0
4	396.5
5	368.5
6	666.0
7	1451.0
8	2130.5
9	2030.0
10	1416.0
11	877.0
12	756.0
13	764.0
14	772.5
15	813.0
16	872.0
17	963.0
18	1010.0
19	1053.0
20	1128.0
21	1157.0
22	1216.0
23	1363.5
24	1506.5
25	1700.5
26	2116.5
27	2750.5
28	3592.0
29	4961.5
30	7075.0
31	9807.0
32	13441.0
33	17900.0
34	22937.5
35	28654.0
36	35089.0
37	42000.0
38	49102.5
39	56345.5
40	64306.5
41	71967.5
42	79674.0
43	88433.0
44	96037.0
45	103586.5
46	110415.5
47	116163.0
48	123035.5
49	126494.5
50	125406.0
51	123992.0
52	123192.0
53	121882.5
54	119173.5
55	113542.5
56	105120.5
57	95529.5
58	87024.5
59	78130.0
60	66900.5
61	56014.5
62	45441.5
63	34488.0
64	25263.0
65	18802.5
66	13968.0
67	10597.5
68	8073.5
69	6001.5
70	4432.0
71	3217.0
72	2296.0
73	1701.5
74	1279.5
75	891.5
76	579.5
77	320.0
78	179.0
79	97.0
80	53.5
81	20.0
82	11.0
83	10.0
84	4.5
85	1.0
86	1.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3146611854798017
2	0.0
3	0.006678341396812205
4	0.06073580481434211
5	5.565284497343504E-4
6	0.063407141373067
7	0.07513134071413731
8	0.10481285803330266
9	0.09360808524531775
10-11	0.09793045620492119
12-13	0.09659478792555876
14-15	0.06567035706865336
16-17	0.03702769285565878
18-19	0.0390682971713514
20-21	0.028920928437861745
22-23	0.04329791338933246
24-25	0.035413760351429166
26-27	0.021853017126235493
28-29	0.02428319135674216
30-31	0.003209314060134754
32-33	0.005806446825561723
34-35	0.00786560208957882
36-37	0.010740999079872963
38-39	0.016974117716897687
40-41	0.021296488676501146
42-43	0.0030794574218634056
44-45	0.01382045650173637
46-47	0.02415333471847081
48-49	0.0026898875070493606
50-51	0.006121812947077855
52-53	0.0020220533673681396
54-55	0.0038029444065180613
56-57	0.003283517853432667
58-59	9.832002611973524E-4
60-61	0.00515716363420498
62-63	0.01471090202131133
64-65	0.006641239500163248
66-67	0.038882787688106615
68-69	0.061960167403757684
70-71	0.012596093912320798
72-73	0.03242705767118815
74-75	0.052016859101837294
76-77	0.305868035973999
78-79	0.07309073639844468
80-81	0.033744175002226116
82-83	0.028624113264670088
84-85	0.019608352378973615
86-87	0.11766866522216615
88-89	0.5279228874180049
90-91	0.13377088836781337
92-93	0.04708230684752605
94-95	0.00773574545130747
96-97	0.005453978807396634
98-99	0.010629693389926092
100-101	0.007197767949897599
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2695280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.873840275309995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24396149207708	44.29900970566122
2	14.35046580493416	16.897340633519832
3	4.553090924380617	8.041738435228437
4	1.971991458949605	4.643948407138984
5	1.0678141069963878	3.143315858951406
6	0.6474134096108106	2.2869428201712427
7	0.4145929919635367	1.7086077111686901
8	0.2901794578493607	1.366718324207945
9	0.22293369228110188	1.1812466332208431
>10	1.159956671897719	12.06242813236054
>50	0.059723633564628294	2.3665297272986514
>100	0.017371983734451178	1.5745756556176993
>500	3.152323502706265E-4	0.1259780781987044
>1k	1.891394101541087E-4	0.30161987725585115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3545	0.13152622362055147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.420379329791338E-5	7.420379329791338E-5	0.0	0.0	0.0
6	1.1130568994687008E-4	7.420379329791338E-5	0.0	0.0	0.0
7	1.1130568994687008E-4	7.420379329791338E-5	0.0	0.0	0.0
8	1.1130568994687008E-4	7.420379329791338E-5	0.0	0.0	0.0
9	1.1130568994687008E-4	7.420379329791338E-5	0.0	0.0	7.420379329791338E-5
10-11	1.1130568994687008E-4	7.420379329791338E-5	0.0	0.0	7.420379329791338E-5
12-13	1.1130568994687008E-4	1.1130568994687008E-4	0.0	0.0	7.420379329791338E-5
14-15	1.1130568994687008E-4	1.4840758659582677E-4	0.0	3.710189664895669E-5	1.2985663827134842E-4
16-17	1.1130568994687008E-4	1.4840758659582677E-4	0.0	5.565284497343504E-5	2.2261137989374016E-4
18-19	1.1130568994687008E-4	2.0406043156926182E-4	0.0	7.420379329791338E-5	2.411623282182185E-4
20-21	1.1130568994687008E-4	2.2261137989374016E-4	0.0	1.8550948324478348E-4	2.5971327654269685E-4
22-23	1.1130568994687008E-4	2.2261137989374016E-4	0.0	2.782642248671752E-4	2.5971327654269685E-4
24-25	1.1130568994687008E-4	2.2261137989374016E-4	0.0	4.4522275978748033E-4	2.5971327654269685E-4
26-27	1.2985663827134842E-4	2.2261137989374016E-4	0.0	8.162417262770473E-4	2.968151731916536E-4
28-29	1.6695853492030514E-4	2.2261137989374016E-4	0.0	0.0018550948324478347	3.3391706984061027E-4
30-31	1.8550948324478348E-4	2.2261137989374016E-4	0.0	0.006233118637024724	3.3391706984061027E-4
32-33	1.8550948324478348E-4	2.2261137989374016E-4	0.0	0.016343385473865425	3.7101896648956696E-4
34-35	1.8550948324478348E-4	2.411623282182185E-4	0.0	0.0303308005105221	3.7101896648956696E-4
36-37	1.8550948324478348E-4	2.5971327654269685E-4	0.0	0.05129337211718263	3.7101896648956696E-4
38-39	1.8550948324478348E-4	2.5971327654269685E-4	0.0	0.08303404470036507	3.7101896648956696E-4
40-41	1.8550948324478348E-4	2.5971327654269685E-4	0.0	0.11707503487578286	3.7101896648956696E-4
42-43	1.8550948324478348E-4	2.5971327654269685E-4	0.0	0.15007717194502984	3.7101896648956696E-4
44-45	2.0406043156926182E-4	2.5971327654269685E-4	0.0	0.1868451515241459	3.895699148140453E-4
46-47	2.2261137989374016E-4	2.5971327654269685E-4	0.0	0.22337196877504378	4.0812086313852364E-4
48-49	2.2261137989374016E-4	2.5971327654269685E-4	0.0	0.26091908818378795	4.0812086313852364E-4
50-51	2.2261137989374016E-4	2.5971327654269685E-4	0.0	0.29638850138019057	4.4522275978748033E-4
52-53	2.2261137989374016E-4	2.5971327654269685E-4	0.0	0.33358315277076966	4.6377370811195867E-4
54-55	2.2261137989374016E-4	4.26671811463002E-4	0.0	0.37324508028850434	4.82324656436437E-4
56-57	2.2261137989374016E-4	5.565284497343504E-4	0.0	0.41389020806743637	4.82324656436437E-4
58-59	2.2261137989374016E-4	5.936303463833071E-4	0.0	0.4535521355851711	4.82324656436437E-4
60-61	2.2261137989374016E-4	5.936303463833071E-4	0.0	0.4934552254311241	4.82324656436437E-4
62-63	2.411623282182185E-4	5.936303463833071E-4	0.0	0.5326719301890712	5.194265530853937E-4
64-65	2.5971327654269685E-4	6.121812947077855E-4	0.0	0.5739848921076846	5.194265530853937E-4
66-67	2.5971327654269685E-4	6.678341396812205E-4	0.0	0.6175054168769107	5.194265530853937E-4
68-69	2.5971327654269685E-4	6.678341396812205E-4	0.0	0.6616937757858181	5.194265530853937E-4
70-71	2.5971327654269685E-4	7.049360363301772E-4	0.0	0.7068653349559229	5.565284497343504E-4
72-73	2.5971327654269685E-4	7.420379329791339E-4	0.0	0.7515360185212667	7.420379329791339E-4
74-75	2.5971327654269685E-4	7.420379329791339E-4	0.0	0.7960397435516904	7.420379329791339E-4
76-77	2.5971327654269685E-4	7.420379329791339E-4	0.0	0.8438640883321955	7.420379329791339E-4
78-79	2.5971327654269685E-4	7.420379329791339E-4	0.0	0.8950090528627823	7.420379329791339E-4
80-81	2.5971327654269685E-4	7.420379329791339E-4	0.0	0.9463766287732629	7.976907779525689E-4
82-83	2.5971327654269685E-4	7.420379329791339E-4	0.0	0.997224778130658	8.904455195749607E-4
84-85	2.5971327654269685E-4	7.420379329791339E-4	0.0	1.0485923540411386	8.904455195749607E-4
86-87	2.5971327654269685E-4	7.605888813036123E-4	0.0	1.1007019678845982	8.904455195749607E-4
88-89	2.5971327654269685E-4	7.791398296280906E-4	0.0	1.1573565640675552	8.904455195749607E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4650	0.0	41.374557	1
GGTATCA	2730	0.0	38.02759	1
TCAACGC	5960	0.0	32.197086	4
CAACGCA	6015	0.0	31.962637	5
TATCAAC	6080	0.0	31.56103	2
ATCAACG	6220	0.0	30.774862	3
AACGCAG	6465	0.0	30.0338	6
GTACATG	7560	0.0	26.204536	1
TACATGG	7600	0.0	25.373817	2
ACGCAGA	7625	0.0	25.093021	7
CGCAGAG	8195	0.0	23.290184	8
ACATGGG	7955	0.0	22.749207	3
GTACCTG	2605	0.0	21.754253	1
TACCTGG	2705	0.0	20.193144	2
GCAGAGT	9360	0.0	19.884108	9
AGAGTAC	7660	0.0	19.214306	10-11
CATGGGG	6160	0.0	19.122843	4
GAGTACG	420	1.8189894E-12	18.141602	1
GAGTACA	5770	0.0	17.662123	1
GAGTACT	3950	0.0	17.606123	12-13
>>END_MODULE