FastQCFastQC Report
Mon 15 Aug 2016
SRR2921446_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921446_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804388
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41770.14894515309579132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37710.13446784111185756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG83950.024.7318341
GTACCTG31150.024.38521
TACATGG86050.023.3370062
GTATAGG5550.022.3960421
TACCTGG32000.022.3509792
ACATGGG86800.021.973313
GTATCAA100200.019.5263791
GAGTACA68600.019.1929781
GTATAAG6750.019.0965421
AGTACAT62650.019.0271682
GTATACG1701.0121207E-418.9561271
CATGGGG69350.018.8413724
ACCTGGG39400.017.4548823
ATACCGT3502.1100277E-917.0250636
TCAACGC113100.016.9854
TATCAAC115150.016.9616282
CAACGCA113850.016.832845
AGAGTAC78850.016.79117210-11
ATCAACG116150.016.5787563
AACGCAG117500.016.3841846
Produced by FastQC (version 0.11.5)