##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921446_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2804388
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.46510575569429	31.0	31.0	34.0	27.0	34.0
2	30.5664201957789	31.0	31.0	34.0	27.0	34.0
3	30.702877062660374	31.0	31.0	34.0	27.0	34.0
4	34.224304910732755	37.0	35.0	37.0	30.0	37.0
5	34.21425387642509	37.0	35.0	37.0	30.0	37.0
6	34.31685308880226	37.0	35.0	37.0	31.0	37.0
7	34.202828923815105	37.0	35.0	37.0	30.0	37.0
8	34.18307916023033	37.0	35.0	37.0	30.0	37.0
9	35.64196145469172	39.0	37.0	39.0	30.0	39.0
10-11	35.51500380831754	39.0	36.0	39.0	30.0	39.0
12-13	35.37066679075791	39.0	35.0	39.0	30.0	39.0
14-15	36.40311433367994	40.0	36.5	41.0	30.0	41.0
16-17	36.262641082475035	40.0	36.0	41.0	29.5	41.0
18-19	36.08584653764029	39.0	36.0	41.0	28.5	41.0
20-21	35.97170398675219	39.0	36.0	41.0	27.5	41.0
22-23	36.01769138221958	39.0	36.0	41.0	28.5	41.0
24-25	35.89475564722143	39.0	36.0	41.0	27.0	41.0
26-27	35.635568437748276	39.0	36.0	41.0	27.0	41.0
28-29	35.41431766930967	39.0	35.5	40.0	26.5	41.0
30-31	35.163933984883684	39.0	35.0	40.0	25.0	41.0
32-33	34.90015522103218	38.5	35.0	40.0	24.5	41.0
34-35	34.486776437497234	38.0	34.0	40.0	23.0	41.0
36-37	34.11743917032878	38.0	34.0	40.0	21.0	41.0
38-39	33.888733834262595	38.0	33.0	40.0	19.0	41.0
40-41	33.627293726830956	38.0	33.0	40.0	18.0	41.0
42-43	33.33571888055433	38.0	33.0	40.0	15.5	41.0
44-45	33.026223011937006	38.0	32.0	40.0	14.5	41.0
46-47	32.74159620566056	37.0	32.0	40.0	11.0	41.0
48-49	32.535723837072474	37.0	31.0	40.0	9.0	41.0
50-51	31.77720878851286	36.0	30.5	39.0	8.5	40.0
52-53	32.29157966016115	37.0	31.5	39.0	8.5	40.0
54-55	32.73096090840497	37.0	32.0	40.0	8.0	40.5
56-57	32.57789756624262	37.0	32.0	40.0	7.0	41.0
58-59	32.2522109993339	36.5	31.5	39.5	4.0	41.0
60-61	31.78621699279843	36.0	31.0	39.0	2.0	40.5
62-63	31.30431915983095	35.5	31.0	39.0	2.0	40.0
64-65	30.763823158564364	35.0	30.0	38.5	2.0	40.0
66-67	30.2678497055329	35.0	29.0	38.0	2.0	40.0
68-69	29.748422115627367	34.0	29.0	37.0	2.0	39.5
70-71	29.224909142386856	34.0	28.5	36.5	2.0	39.0
72-73	28.68392230319057	34.0	27.5	36.0	2.0	39.0
74-75	28.09329967893173	33.5	26.0	35.5	2.0	37.5
76-77	27.51619925630833	33.0	26.0	35.0	2.0	37.0
78-79	26.955427886583458	33.0	25.0	35.0	2.0	36.5
80-81	26.38281881822344	32.0	24.5	35.0	2.0	36.0
82-83	25.759313261930945	32.0	22.0	34.5	2.0	35.5
84-85	25.148798953639798	31.0	20.0	34.0	2.0	35.0
86-87	24.53095898285116	31.0	17.5	34.0	2.0	35.0
88-89	23.88077683972403	31.0	9.5	34.0	2.0	35.0
90-91	23.14028390508018	30.0	2.0	34.0	2.0	35.0
92-93	22.31764827120926	29.5	2.0	34.0	2.0	35.0
94-95	21.392917278208294	29.0	2.0	34.0	2.0	35.0
96-97	20.47770101712031	29.0	2.0	33.5	2.0	34.5
98	19.847811715069383	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81252.0
3	14424.0
4	10959.0
5	9152.0
6	8791.0
7	9033.0
8	10122.0
9	12089.0
10	13363.0
11	14512.0
12	16259.0
13	18608.0
14	15704.0
15	15644.0
16	17526.0
17	20320.0
18	23084.0
19	22424.0
20	23116.0
21	25368.0
22	27916.0
23	31225.0
24	35176.0
25	39521.0
26	44834.0
27	51960.0
28	59913.0
29	71227.0
30	84428.0
31	102559.0
32	129025.0
33	166832.0
34	226964.0
35	317220.0
36	424678.0
37	428615.0
38	173813.0
39	6732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.152474164861204	16.963966775382712	13.041124474796877	24.842434584959207
2	16.19509807454172	18.499433290011027	38.58747401464348	26.71799462080377
3	20.533781558658134	21.68746701908157	27.774295095864538	30.00445632639576
4	14.041803343611011	14.32951173061019	34.262379773643715	37.36630515213509
5	15.586204337264103	35.59791529289521	32.71906123952009	16.0968191303206
6	35.7276639656481	32.70413837816853	16.15836872703632	15.409828929147057
7	31.60012642847409	30.20463290436599	18.87697696105047	19.318263706109448
8	28.27679576750223	33.45639124211926	18.51378953566712	19.753023454711396
9	26.972353193542624	16.439655655090192	18.85580458829357	37.73218656307361
10-11	26.982044626217842	24.693440162454824	25.75081237220172	22.573702839125605
12-13	26.998089576978778	21.74257183522218	26.3947033102706	24.86463527752844
14-15	24.088072441716005	23.40639476712348	23.969704324002674	28.53582846715784
16-17	24.50490997633725	26.572574389663284	24.56011711902881	24.362398514970657
18-19	24.47104277272112	25.956798607988052	25.924099742066065	23.648058877224766
20-21	25.000285506681568	25.00660234201131	25.871170106308412	24.121942044998708
22-23	24.800424191175868	24.677082504184266	25.780161602417508	24.742331702222366
24-25	24.61275585375697	24.856562034890423	25.93064735581358	24.60003475553903
26-27	24.57399298388621	24.993464439502304	26.02500340384482	24.407539172766672
28-29	24.53191059565541	25.334556065443646	25.802342106596825	24.33119123230411
30-31	24.714160148811015	25.13606727964807	25.586161017957544	24.563611553583364
32-33	24.606134237976875	25.215309716220464	25.55820723773093	24.620348808071736
34-35	24.848364209442483	25.096770521042842	25.70127301745736	24.353592252057314
36-37	24.775222660268568	25.04491599712796	25.72424466309683	24.455616679506644
38-39	24.725863920912523	25.209742119316214	25.715084970976992	24.34930898879427
40-41	24.841578654808227	25.1075866520827	25.618354282845306	24.432480410263764
42-43	24.72601058832366	25.043287431954163	25.54742759091347	24.68327438880871
44-45	24.71058491707748	25.06486611662207	25.51430460081618	24.71024436548427
46-47	24.91561027548283	25.131748332860493	25.541640000379946	24.41100139127673
48-49	24.798357795855246	25.185630534531718	25.393128153389465	24.622883516223578
50-51	24.568361247770877	25.370684015736032	25.554157593354304	24.506797143138783
52-53	24.74639099884682	25.393043503528865	25.32861322983832	24.531952267785993
54-55	24.662137324146975	25.09866992404433	25.50507704054424	24.734115711264458
56-57	24.696200401176608	25.218651288767752	25.47772584377127	24.60742246628437
58-59	24.863395028675754	25.174774798022483	25.39205608849952	24.569774084802244
60-61	24.816478483817185	25.112378308732662	25.571139533345722	24.500003674104427
62-63	24.583869132483965	25.309030786388842	25.535401819787218	24.57169826133997
64-65	24.634004360448177	25.28862735522378	25.462009043958915	24.615359240369123
66-67	24.739352402867873	25.182398174879523	25.357869836788936	24.720379585463668
68-69	24.535980915668627	25.220616426484405	25.43122662786898	24.812176029977987
70-71	24.638364531991463	25.07429417843215	25.53243862623668	24.7549026633397
72-73	24.72803565373632	25.047140247857307	25.47959279996862	24.74523129843776
74-75	24.505038442885827	25.44411866469623	25.37681764683549	24.674025245582452
76-77	24.73048522063682	25.087778468156248	25.346953989090988	24.83478232211595
78-79	24.618169534947366	25.071462242861298	25.509889371433157	24.800478850758182
80-81	24.684406909216865	25.194542459417864	25.405481109546614	24.715569521818665
82-83	24.764107723975183	25.09823956068641	25.38946967923308	24.748183036105335
84-85	24.698289894551436	25.199531403386686	25.347892873169837	24.75428582889204
86-87	24.64386892972648	25.169281792492367	25.469003506333966	24.717845771447195
88-89	24.583780921658473	25.01674369932669	25.382104742564394	25.017370636450448
90-91	24.676865811040802	24.947744951305335	25.373987829870348	25.00140140778352
92-93	24.798585500448343	25.149344998811756	25.412172855340987	24.63989664539891
94-95	24.87685986338121	24.976801241852225	25.35892477405278	24.787414120713784
96-97	24.66358573477432	24.931101699407836	25.50277670718061	24.902535858637236
98	24.719271759550278	25.168315164930416	25.146215796571198	24.966197278948112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	456.0
1	358.0
2	393.5
3	546.0
4	468.5
5	423.5
6	711.5
7	1381.5
8	2010.5
9	2004.0
10	1597.0
11	1357.0
12	1404.5
13	1573.5
14	1764.0
15	2021.5
16	2209.0
17	2278.0
18	2270.5
19	2171.0
20	2069.5
21	1919.0
22	1767.5
23	1748.0
24	1850.0
25	2080.0
26	2447.0
27	3122.0
28	4203.0
29	5715.5
30	7897.0
31	10825.5
32	14778.5
33	19210.0
34	24399.0
35	30421.5
36	37009.0
37	44403.5
38	51900.5
39	59903.0
40	67846.5
41	75480.0
42	84197.0
43	92999.0
44	101483.0
45	110222.0
46	120676.5
47	131809.5
48	136015.0
49	133062.0
50	130877.0
51	130036.5
52	130036.5
53	131000.0
54	128892.0
55	123594.5
56	115926.0
57	107340.5
58	98412.5
59	87668.0
60	75769.5
61	63338.5
62	51054.5
63	39023.0
64	28491.5
65	21361.5
66	16402.5
67	12412.0
68	9259.5
69	6964.5
70	5201.5
71	3888.5
72	2970.5
73	2131.5
74	1601.5
75	1171.5
76	742.5
77	419.5
78	238.5
79	133.0
80	77.0
81	49.0
82	30.5
83	20.0
84	14.5
85	13.0
86	12.0
87	10.5
88	9.0
89	6.5
90	2.5
91	2.0
92	2.5
93	5.0
94	6.5
95	4.5
96	4.5
97	4.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4751482319850178
2	0.04874503813309713
3	0.0581588567630442
4	0.055270526047037714
5	0.06718043295007681
6	0.06650292327595182
7	0.04357456956740651
8	0.019933047780834892
9	0.012088198922545668
10-11	0.014566457993687035
12-13	0.010590545958690453
14-15	0.015101334052206754
16-17	0.014370336772229805
18-19	0.0548604544021726
20-21	0.08386856597589207
22-23	0.10059235740560865
24-25	0.06978349643487278
26-27	0.08591892420021766
28-29	0.08793362402064193
30-31	0.05842629479230406
32-33	0.03303751121456803
34-35	0.3987322724245005
36-37	0.6246995779471314
38-39	0.5734941099448436
40-41	0.5683236413791529
42-43	0.541419375635611
44-45	0.5276017441238516
46-47	0.5172073193866183
48-49	0.5076152087371648
50-51	0.521076256209911
52-53	0.6018603702483394
54-55	0.5723530410200015
56-57	0.5292955183091641
58-59	0.5072764539001023
60-61	0.5203452589299341
62-63	0.5327722126895422
64-65	0.5510828030928674
66-67	0.5772560715564322
68-69	0.5304009288301048
70-71	0.47981948289608994
72-73	0.4629708870527188
74-75	0.44408976218697266
76-77	0.4579787105065348
78-79	0.45300436316230136
80-81	0.44861837948243966
82-83	0.4679987220028042
84-85	0.43578135407796637
86-87	0.43852705117836766
88-89	0.4649677576711924
90-91	0.4476021149712522
92-93	0.4438223241577129
94-95	0.45469813734761383
96-97	0.4490106219253541
98	0.44405410378307136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2804388.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.496157522535896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6985486532817	44.865703837722506
2	13.097436710943672	15.322994419696109
3	4.299271050311463	7.544725097732931
4	1.9205965291134033	4.493900684170134
5	1.050522828245814	3.0725774421043535
6	0.6342577774383297	2.226098571535563
7	0.41669881272580034	1.7062695572063473
8	0.3115457533955254	1.4579383572881421
9	0.24079751652766318	1.2677156512053867
>10	1.2483105963581629	12.962821485358111
>50	0.06330500587798062	2.503280936668455
>100	0.017546310421691004	1.6180803632714722
>500	6.118186098129842E-4	0.26423046589146654
>1k	5.506367488293711E-4	0.6936631301490184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4177	0.14894515309579132	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3771	0.13446784111185756	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.565840390131465E-5
5	0.0	0.0	0.0	3.565840390131465E-5	3.565840390131465E-5
6	0.0	0.0	0.0	3.565840390131465E-5	3.565840390131465E-5
7	0.0	0.0	0.0	3.565840390131465E-5	3.565840390131465E-5
8	0.0	0.0	0.0	3.565840390131465E-5	7.13168078026293E-5
9	0.0	0.0	0.0	7.13168078026293E-5	7.13168078026293E-5
10-11	0.0	0.0	0.0	8.914600975328663E-5	8.914600975328663E-5
12-13	0.0	0.0	0.0	1.0697521170394396E-4	2.3177962535854524E-4
14-15	0.0	0.0	0.0	1.0697521170394396E-4	3.922424429144612E-4
16-17	0.0	0.0	0.0	1.426336156052586E-4	4.635592507170905E-4
18-19	0.0	0.0	0.0	1.961212214572306E-4	4.635592507170905E-4
20-21	0.0	3.565840390131465E-5	0.0	2.1395042340788793E-4	5.170468565690625E-4
22-23	0.0	3.565840390131465E-5	0.0	3.7441324096380385E-4	5.527052604703772E-4
24-25	0.0	5.348760585197198E-5	0.0	4.635592507170905E-4	5.705344624210344E-4
26-27	0.0	7.13168078026293E-5	0.0	6.418512702236638E-4	5.705344624210344E-4
28-29	0.0	1.0697521170394396E-4	0.0	0.0016224573775098167	5.705344624210344E-4
30-31	0.0	1.7829201950657326E-4	0.0	0.006044099461272833	6.061928663223491E-4
32-33	0.0	1.7829201950657326E-4	0.0	0.014780408417094923	6.953388760756357E-4
34-35	0.0	1.7829201950657326E-4	0.0	0.025477929587489317	7.13168078026293E-4
36-37	0.0	1.7829201950657326E-4	0.0	0.04314666872059073	7.13168078026293E-4
38-39	0.0	1.961212214572306E-4	0.0	0.06919513277050109	7.13168078026293E-4
40-41	0.0	2.1395042340788793E-4	0.0	0.09461957475213845	7.13168078026293E-4
42-43	0.0	2.1395042340788793E-4	0.0	0.11977657870451593	7.309972799769504E-4
44-45	0.0	2.1395042340788793E-4	0.0	0.14994358840502814	7.66655683878265E-4
46-47	0.0	2.1395042340788793E-4	0.0	0.18289195360984287	8.023140877795798E-4
48-49	0.0	2.1395042340788793E-4	0.0	0.21293415889670045	8.558016936315517E-4
50-51	0.0	2.1395042340788793E-4	0.0	0.24244148812503835	8.914600975328664E-4
52-53	0.0	2.1395042340788793E-4	0.0	0.2731968614899222	0.0010697521170394395
54-55	0.0	2.1395042340788793E-4	0.0	0.30375611363334887	0.0010697521170394395
56-57	0.0	2.1395042340788793E-4	0.0	0.3396819555639234	0.0010697521170394395
58-59	0.0	2.1395042340788793E-4	0.0	0.3727729543843434	0.0010697521170394395
60-61	0.0	2.1395042340788793E-4	0.0	0.4078073362173851	0.0011054105209407543
62-63	0.0	2.1395042340788793E-4	0.0	0.4424138172036109	0.0011410689248420689
64-65	0.0	2.1395042340788793E-4	0.0	0.4757365956493894	0.0011767273287433837
66-67	0.0	2.1395042340788793E-4	0.0	0.5133027241594244	0.0011767273287433837
68-69	1.7829201950657326E-5	2.1395042340788793E-4	0.0	0.550993657083114	0.0011767273287433837
70-71	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.5900574385570042	0.0012302149345953554
72-73	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.6275700794611873	0.0012837025404473276
74-75	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.6671865661955478	0.0012837025404473276
76-77	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.7066247609104019	0.0013193609443486421
78-79	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.7493435287841768	0.001355019348249957
80-81	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.792365393091113	0.001372848550200614
82-83	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.8331942655581182	0.0014976529638552154
84-85	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.8779099040503668	0.0014976529638552154
86	3.565840390131465E-5	2.1395042340788793E-4	0.0	0.9111791948902933	0.0015333113677565302
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8395	0.0	24.731834	1
GTACCTG	3115	0.0	24.3852	1
TACATGG	8605	0.0	23.337006	2
GTATAGG	555	0.0	22.396042	1
TACCTGG	3200	0.0	22.350979	2
ACATGGG	8680	0.0	21.97331	3
GTATCAA	10020	0.0	19.526379	1
GAGTACA	6860	0.0	19.192978	1
GTATAAG	675	0.0	19.096542	1
AGTACAT	6265	0.0	19.027168	2
GTATACG	170	1.0121207E-4	18.956127	1
CATGGGG	6935	0.0	18.841372	4
ACCTGGG	3940	0.0	17.454882	3
ATACCGT	350	2.1100277E-9	17.025063	6
TCAACGC	11310	0.0	16.985	4
TATCAAC	11515	0.0	16.961628	2
CAACGCA	11385	0.0	16.83284	5
AGAGTAC	7885	0.0	16.791172	10-11
ATCAACG	11615	0.0	16.578756	3
AACGCAG	11750	0.0	16.384184	6
>>END_MODULE