FastQCFastQC Report
Mon 15 Aug 2016
SRR2921446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804388
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42700.15226138465861358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.11842155935626597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG32750.026.6175881
GTACATG85500.025.7397771
TACCTGG33400.024.7464082
TACATGG89100.023.7775172
ACATGGG90250.022.1068533
CATGGGG69800.019.8728474
GTATCAA101050.019.5632361
GAGTACA67300.019.4646471
TACCGTA2001.2903665E-518.992217
ACCTGGG44450.018.5952633
AGTACAT63750.018.4791012
TCAACGC110850.017.1846754
CAACGCA111550.017.109955
GTATAGT5050.016.9789181
GTATAGA7600.016.9230671
TATCAAC116150.016.8086172
AGAGTAC84050.016.69258710-11
AACGCAG116900.016.5714746
ATCAACG117000.016.5246933
GGTATCA81350.016.2785661
Produced by FastQC (version 0.11.5)