##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921446_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2804388 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00344888082534 33.0 31.0 34.0 30.0 34.0 2 32.11869327639399 34.0 31.0 34.0 30.0 34.0 3 32.24356579760005 34.0 31.0 34.0 30.0 34.0 4 35.773870805323654 37.0 35.0 37.0 35.0 37.0 5 35.58959530564244 37.0 35.0 37.0 33.0 37.0 6 35.6298058613858 37.0 35.0 37.0 35.0 37.0 7 35.559141958958605 37.0 35.0 37.0 33.0 37.0 8 35.513600471832 37.0 35.0 37.0 33.0 37.0 9 37.146345655451384 39.0 37.0 39.0 34.0 39.0 10-11 37.058937458012224 39.0 37.0 39.0 33.5 39.0 12-13 36.95179803935832 39.0 37.0 39.0 33.0 39.0 14-15 38.27264540427359 40.0 38.0 41.0 34.0 41.0 16-17 38.18527250865429 40.0 38.0 41.0 33.0 41.0 18-19 38.07922958592035 40.0 38.0 41.0 33.0 41.0 20-21 37.9619653200627 40.0 38.0 41.0 33.0 41.0 22-23 37.93626951762738 40.0 38.0 41.0 33.0 41.0 24-25 37.99682176646028 40.0 38.0 41.0 33.0 41.0 26-27 37.902605844840295 40.0 38.0 41.0 33.0 41.0 28-29 37.77525649089926 40.0 38.0 41.0 33.0 41.0 30-31 37.64439995464251 40.0 38.0 41.0 32.5 41.0 32-33 37.49457564359854 40.0 37.5 41.0 32.0 41.0 34-35 37.36062681055546 40.0 37.0 41.0 32.0 41.0 36-37 37.28075572994892 40.0 37.0 41.0 31.5 41.0 38-39 37.26234850527102 40.0 37.0 41.0 32.0 41.0 40-41 37.10976048963268 40.0 37.0 41.0 31.0 41.0 42-43 36.889305081893085 40.0 36.5 41.0 31.0 41.0 44-45 36.70018877559025 39.5 36.0 41.0 30.5 41.0 46-47 36.45201912146251 39.0 36.0 41.0 30.0 41.0 48-49 36.14233087575613 39.0 35.0 40.0 29.5 41.0 50-51 35.877304246060106 39.0 35.0 40.0 29.0 41.0 52-53 35.58444123994255 38.0 35.0 40.0 28.0 41.0 54-55 35.268177406264755 38.0 34.5 40.0 28.0 41.0 56-57 34.89633121379781 38.0 34.0 40.0 27.0 41.0 58-59 34.52141643738313 37.5 34.0 40.0 26.0 41.0 60-61 34.098167051064266 37.0 33.0 39.5 26.0 41.0 62-63 33.6683743476295 36.0 33.0 39.0 25.5 40.5 64-65 33.16972330504909 35.5 32.5 39.0 24.0 40.0 66-67 32.65081846734475 35.0 32.0 38.0 22.5 40.0 68-69 32.15647674287581 35.0 31.0 37.5 22.0 39.5 70-71 31.614209588687444 35.0 31.0 37.0 20.0 39.0 72-73 31.081329509326096 34.0 30.0 36.0 18.5 39.0 74-75 30.576250682858436 34.0 30.0 36.0 17.5 38.0 76-77 29.255841738019132 32.5 28.5 34.5 16.5 36.0 78-79 30.193138217678865 34.0 30.0 35.0 17.5 37.0 80-81 30.24049293464385 34.0 30.0 35.0 17.0 36.5 82-83 29.923052017053273 34.0 30.0 35.0 12.5 36.0 84-85 29.463499522890555 34.0 29.5 35.0 7.0 36.0 86-87 28.985294117647058 34.0 29.0 35.0 4.0 35.0 88-89 28.468171308677686 33.0 29.0 35.0 2.0 35.0 90-91 28.033185315298738 33.0 27.5 35.0 2.0 35.0 92-93 27.63033628014383 33.0 27.0 35.0 2.0 35.0 94-95 27.16715340387992 33.0 26.5 34.0 2.0 35.0 96-97 26.452208289295207 32.0 25.0 34.0 2.0 35.0 98-99 25.50344371035677 32.0 23.0 34.0 2.0 35.0 100-101 21.906174716194762 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16146.0 3 8665.0 4 5080.0 5 3924.0 6 3395.0 7 3417.0 8 3854.0 9 4769.0 10 5758.0 11 6651.0 12 7720.0 13 9142.0 14 10084.0 15 11549.0 16 13065.0 17 15236.0 18 15526.0 19 14389.0 20 14347.0 21 15117.0 22 16190.0 23 18499.0 24 21227.0 25 24821.0 26 29615.0 27 35511.0 28 44039.0 29 53772.0 30 66640.0 31 83504.0 32 107939.0 33 145424.0 34 208515.0 35 320176.0 36 505965.0 37 640935.0 38 284901.0 39 8881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4754858526894 17.33899137521825 13.383668109555583 24.80185466253677 2 16.286548081078653 18.772010149808086 38.17485312303433 26.766588646078933 3 20.55006677471868 21.767310164556445 28.02708789122051 29.655535169504372 4 14.246629400101545 14.592083108698034 34.246622264102605 36.914665227097814 5 15.637284407752478 35.53075060049181 32.8632690146229 15.968695977132816 6 35.8304179429862 32.84310769609531 16.01438635864921 15.312088002269277 7 31.66127890931189 30.243535672496673 18.828864866909488 19.266320551281947 8 28.32267540955519 33.57754643469694 18.47150559607926 19.62827255966861 9 26.853464875134154 16.476702689333145 18.79663664718309 37.87319578834961 10-11 26.99994271358775 24.738057433822146 25.793662195411628 22.468337657178477 12-13 26.998370658286174 21.652273850760864 26.509425375971045 24.83993011498191 14-15 23.98826317128441 23.414177606684575 23.998503548986648 28.599055673044365 16-17 24.458320291285773 26.585712336573113 24.706765723804658 24.24920164833646 18-19 24.496996883179424 25.987541635639662 25.93451555511957 23.580945926061347 20-21 25.022707334955168 24.97755126025932 25.894797403133353 24.104944001652157 22-23 24.82641072002206 24.60279451584693 25.88514970442243 24.685645059708577 24-25 24.63675373733266 24.908827274770196 25.93899702868231 24.51542195921483 26-27 24.54449773666573 25.031831481817 26.069270297607417 24.354400483909856 28-29 24.511581498492628 25.32566137636905 25.90927937639937 24.25347774873895 30-31 24.72630414945054 25.079832785305705 25.629132271558774 24.56473079368498 32-33 24.555998159215715 25.117415969641684 25.675125109677687 24.651460761464918 34-35 24.808931706112418 25.014696875275817 25.847867504348436 24.328503914263326 36-37 24.70447138648233 25.04254952283017 25.841473093455956 24.41150599723154 38-39 24.63828202081674 25.17099565594267 25.84151510790777 24.349207215332825 40-41 24.774454184565677 25.125244778577148 25.716921923547233 24.383379113309942 42-43 24.638245771250546 25.104995513118933 25.559906236399172 24.69685247923135 44-45 24.600702784497372 25.048957039417775 25.60077803443594 24.749562141648916 46-47 24.803554301974426 25.15414446832954 25.668963987244002 24.37333724245203 48-49 24.72978230318798 25.230047430511505 25.505177203828534 24.534993062471987 50-51 24.48691975689245 25.419434696138744 25.587130120594498 24.50651542637431 52-53 24.727531743463427 25.380239577009377 25.338785640356342 24.55344303917085 54-55 24.643410507172085 25.09652227402805 25.527061361671024 24.73300585712885 56-57 24.66894876825175 25.210548208965093 25.535372366553844 24.58513065622931 58-59 24.88212980092399 25.217358281188268 25.37914230575576 24.521369612131984 60-61 24.814418786187513 25.179117765105257 25.61046604407163 24.3959974046356 62-63 24.532703655934228 25.364807930739765 25.535226680832267 24.56726173249374 64-65 24.641718346891352 25.308454314776473 25.437368845512427 24.612458492819748 66-67 24.740671800851924 25.27159944650967 25.308377516363745 24.679351236274663 68-69 24.4953657228154 25.2804623911989 25.48398416711907 24.740187718866636 70-71 24.65530654861835 25.135255279908574 25.498979244125326 24.71045892734775 72-73 24.762821480025355 25.13787333523811 25.41322763609073 24.68607754864581 74-75 24.505249593379045 25.485171312378725 25.293928352656224 24.715650741586007 76-77 24.641794610788295 25.126739969712595 25.41242799231155 24.819037427187556 78-79 24.603964476792047 25.162980041301243 25.509369501241284 24.723685980665422 80-81 24.655708700739094 25.254704933938303 25.431398567474243 24.658187797848356 82-83 24.691908685017527 25.225153084836688 25.416193687206682 24.666744542939103 84-85 24.60595084309436 25.30162227632267 25.35399691422971 24.738429966353262 86-87 24.5890858928296 25.30848903562636 25.483654115281578 24.61877095626246 88-89 24.507738180160118 25.1893052736628 25.323138115779837 24.979818430397245 90-91 24.612083516992808 25.115671995513978 25.35091679774999 24.921327689743222 92-93 24.69817386312839 25.361690119339098 25.425103843317622 24.515032174214895 94-95 24.73591368791143 25.138263847915383 25.371971073582205 24.75385139059098 96-97 24.51280813227427 25.102666182636685 25.556943536095776 24.827582148993272 98-99 24.58268279827196 25.258460640875402 25.313647649051283 24.845208911801354 100-101 24.993095120795715 24.690082810407976 25.496148334457548 24.820673734338765 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 171.0 2 264.0 3 387.5 4 318.5 5 285.0 6 542.5 7 1156.0 8 1730.5 9 1715.5 10 1231.0 11 867.5 12 744.5 13 715.0 14 762.5 15 809.5 16 824.5 17 895.0 18 964.5 19 964.0 20 1016.0 21 1120.5 22 1189.0 23 1257.5 24 1401.0 25 1601.0 26 1905.5 27 2367.0 28 3039.0 29 4219.0 30 6111.0 31 8628.0 32 11806.0 33 15726.5 34 20246.0 35 25463.0 36 31784.0 37 39033.0 38 46219.5 39 53504.5 40 61538.0 41 68973.5 42 76529.5 43 85398.5 44 94575.5 45 103471.0 46 111098.0 47 120521.5 48 133702.0 49 137078.5 50 131409.5 51 128878.5 52 128287.5 53 128935.0 54 128303.5 55 123791.0 56 116047.5 57 106560.5 58 97385.0 59 87220.5 60 75536.0 61 63654.0 62 51510.5 63 39118.5 64 28442.0 65 21141.5 66 16040.5 67 12189.5 68 9125.5 69 6864.5 70 5232.5 71 3960.0 72 2788.5 73 1988.5 74 1468.5 75 1059.5 76 699.0 77 365.5 78 199.0 79 94.5 80 50.5 81 30.5 82 16.0 83 9.5 84 6.5 85 3.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3151846320837202 2 0.0 3 0.006525487913940581 4 0.06051231142053096 5 7.131680780262931E-4 6 0.061796013960978294 7 0.0729927527859911 8 0.102446594408477 9 0.09060800431324054 10-11 0.09543971804186867 12-13 0.09424516151117464 14-15 0.06265181565460985 16-17 0.03407160492770615 18-19 0.03745915329833104 20-21 0.02776006743717346 22-23 0.040918018476758564 24-25 0.03273441478140685 26-27 0.019968706184736208 28-29 0.023908959815831476 30-31 0.0031735979472170043 32-33 0.005295272979345227 34-35 0.007862678060239882 36-37 0.010786667180147683 38-39 0.015778843726331735 40-41 0.020628386656910528 42-43 0.003619327995983437 44-45 0.014227703156624548 46-47 0.024497323480203167 48-49 0.002674380292598599 50-51 0.007024705568558986 52-53 0.002513917475042683 54-55 0.004029399640848556 56-57 0.003619327995983437 58-59 0.0011410689248420689 60-61 0.0056161986144570575 62-63 0.014709091609292296 64-65 0.007292143597818847 66-67 0.03876068504072903 68-69 0.06202779358633684 70-71 0.01207036972059501 72-73 0.03241348914629502 74-75 0.05024269109695235 76-77 0.30331038358458245 78-79 0.07313538640159635 80-81 0.03380416689844629 82-83 0.02836626030349581 84-85 0.019612122145723058 86-87 0.11824326733675938 88-89 0.5246777550039438 90-91 0.1315260227899991 92-93 0.04874503813309713 94-95 0.008504529330463544 96-97 0.005491394200802456 98-99 0.010198303515775992 100-101 0.007541752425128049 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2804388.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.30178582453791 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98596069139306 39.63268005662168 2 14.907557676772477 16.1901400826209 3 5.05662488147714 8.237512839270032 4 2.2915261474661803 4.977358482841479 5 1.2468949776656348 3.385431201144564 6 0.7729631338175416 2.518396712569426 7 0.5132536745993513 1.9509413778225733 8 0.36124979526091316 1.569320720913302 9 0.29390700211117227 1.43637075728756 >10 1.466369175073284 14.241624832916171 >50 0.07917193589267371 2.914099118252647 >100 0.023140548851672393 1.9612020778455794 >500 7.887769250776651E-4 0.3053564356414195 >1k 5.915826937673414E-4 0.6795653042525931 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4270 0.15226138465861358 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3321 0.11842155935626597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.13168078026293E-5 2 0.0 0.0 0.0 0.0 7.13168078026293E-5 3 0.0 0.0 0.0 0.0 7.13168078026293E-5 4 0.0 0.0 0.0 0.0 7.13168078026293E-5 5 0.0 0.0 0.0 3.565840390131465E-5 7.13168078026293E-5 6 0.0 0.0 0.0 3.565840390131465E-5 7.13168078026293E-5 7 0.0 0.0 0.0 3.565840390131465E-5 1.0697521170394396E-4 8 0.0 0.0 0.0 3.565840390131465E-5 1.0697521170394396E-4 9 0.0 0.0 0.0 7.13168078026293E-5 1.0697521170394396E-4 10-11 0.0 0.0 0.0 1.0697521170394396E-4 1.0697521170394396E-4 12-13 0.0 0.0 0.0 1.426336156052586E-4 3.7441324096380385E-4 14-15 0.0 0.0 0.0 1.426336156052586E-4 4.100716448651185E-4 16-17 0.0 0.0 0.0 1.6046281755591592E-4 4.2790084681577586E-4 18-19 0.0 0.0 0.0 1.961212214572306E-4 4.2790084681577586E-4 20-21 0.0 0.0 0.0 2.1395042340788793E-4 4.635592507170905E-4 22-23 0.0 0.0 0.0 3.0309643316117456E-4 5.527052604703772E-4 24-25 0.0 0.0 0.0 4.2790084681577586E-4 5.705344624210344E-4 26-27 0.0 0.0 0.0 6.418512702236638E-4 5.705344624210344E-4 28-29 0.0 0.0 0.0 0.0016046281755591595 5.705344624210344E-4 30-31 0.0 0.0 0.0 0.006008441057371519 5.705344624210344E-4 32-33 0.0 0.0 0.0 0.01453079958978572 5.705344624210344E-4 34-35 0.0 0.0 0.0 0.025317466769933406 6.061928663223491E-4 36-37 0.0 0.0 0.0 0.04312883951864008 6.061928663223491E-4 38-39 0.0 0.0 0.0 0.06939125399195832 6.061928663223491E-4 40-41 0.0 0.0 0.0 0.09497615879115158 6.061928663223491E-4 42-43 0.0 0.0 0.0 0.12043625917669025 6.418512702236638E-4 44-45 0.0 0.0 0.0 0.15081721930061032 6.418512702236638E-4 46-47 0.0 0.0 0.0 0.18374775530347442 6.418512702236638E-4 48-49 0.0 0.0 0.0 0.21373647298448004 7.488264819276077E-4 50-51 0.0 0.0 0.0 0.24276241376015018 7.488264819276077E-4 52-53 0.0 0.0 0.0 0.27366042074063934 8.023140877795798E-4 54-55 0.0 0.0 0.0 0.30397006405675675 9.092892994835237E-4 56-57 0.0 0.0 0.0 0.33936102992881156 9.449477033848382E-4 58-59 0.0 0.0 0.0 0.3720241279024158 9.627769053354956E-4 60-61 0.0 3.565840390131465E-5 0.0 0.4063988292632831 9.984353092368104E-4 62-63 0.0 3.565840390131465E-5 0.0 0.4400960209500254 0.001034093713138125 64-65 0.0 3.565840390131465E-5 0.0 0.47299089854898824 0.0010697521170394395 66-67 0.0 3.565840390131465E-5 0.0 0.509736883769293 0.0010697521170394395 68-69 0.0 3.565840390131465E-5 0.0 0.5465898442013017 0.0010697521170394395 70-71 0.0 7.13168078026293E-5 0.0 0.5852078956264255 0.0010697521170394395 72-73 0.0 1.0697521170394396E-4 0.0 0.6220608560584342 0.0012658733384966702 74-75 0.0 1.0697521170394396E-4 0.0 0.6613742463596335 0.001372848550200614 76-77 0.0 1.0697521170394396E-4 0.0 0.7001705898042638 0.001426336156052586 78-79 0.0 1.0697521170394396E-4 0.0 0.7421940188019631 0.001426336156052586 80-81 0.0 1.0697521170394396E-4 0.0 0.7850019326854915 0.0015154821658058728 82-83 0.0 1.0697521170394396E-4 0.0 0.8250998078725198 0.001747261791164418 84-85 0.0 1.0697521170394396E-4 0.0 0.869155765892594 0.001747261791164418 86-87 0.0 1.0697521170394396E-4 0.0 0.9125877018443953 0.0017650909931150754 88-89 0.0 1.0697521170394396E-4 0.0 0.9579986792127195 0.0018185785989670473 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 3275 0.0 26.617588 1 GTACATG 8550 0.0 25.739777 1 TACCTGG 3340 0.0 24.746408 2 TACATGG 8910 0.0 23.777517 2 ACATGGG 9025 0.0 22.106853 3 CATGGGG 6980 0.0 19.872847 4 GTATCAA 10105 0.0 19.563236 1 GAGTACA 6730 0.0 19.464647 1 TACCGTA 200 1.2903665E-5 18.99221 7 ACCTGGG 4445 0.0 18.595263 3 AGTACAT 6375 0.0 18.479101 2 TCAACGC 11085 0.0 17.184675 4 CAACGCA 11155 0.0 17.10995 5 GTATAGT 505 0.0 16.978918 1 GTATAGA 760 0.0 16.923067 1 TATCAAC 11615 0.0 16.808617 2 AGAGTAC 8405 0.0 16.692587 10-11 AACGCAG 11690 0.0 16.571474 6 ATCAACG 11700 0.0 16.524693 3 GGTATCA 8135 0.0 16.278566 1 >>END_MODULE