FastQCFastQC Report
Mon 15 Aug 2016
SRR2921445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172092
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42710.13464300531006035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.045.4880181
GTATCAA54100.039.651561
TCAACGC67750.031.1263584
TATCAAC69300.030.4963232
CAACGCA69750.030.299575
ATCAACG70200.030.0400373
AACGCAG74350.028.3556866
GTACATG91500.024.1485881
ACGCAGA86950.023.9804427
TACATGG93750.022.640682
CGCAGAG92600.022.4621168
ACATGGG97450.020.840193
GAGTACA72450.019.3790781
GTACCTG29500.019.3495711
GCAGAGT109850.018.8514239
GTATAGG5750.018.41331
CATGGGG75650.018.3617254
AGAGTAC91300.017.88949210-11
AGTACAT70050.017.5390642
TACCTGG31950.017.3617822
Produced by FastQC (version 0.11.5)