##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921445_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3172092
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60977203687661	31.0	31.0	34.0	27.0	34.0
2	30.72094094370529	31.0	31.0	34.0	27.0	34.0
3	30.849781784387087	31.0	31.0	34.0	27.0	34.0
4	34.383118774613095	37.0	35.0	37.0	31.0	37.0
5	34.38171780641923	37.0	35.0	37.0	31.0	37.0
6	34.479221914118504	37.0	35.0	37.0	32.0	37.0
7	34.372409438313895	37.0	35.0	37.0	31.0	37.0
8	34.35976573188924	37.0	35.0	37.0	31.0	37.0
9	35.84165906915688	39.0	37.0	39.0	31.0	39.0
10-11	35.72563847454613	39.0	37.0	39.0	30.0	39.0
12-13	35.57925889286943	39.0	35.0	39.0	30.0	39.0
14-15	36.63027159994098	40.0	37.0	41.0	30.0	41.0
16-17	36.495619294774556	40.0	36.5	41.0	30.0	41.0
18-19	36.32336105005781	40.0	36.0	41.0	30.0	41.0
20-21	36.21939653704874	39.0	36.0	41.0	29.0	41.0
22-23	36.26378065327235	39.5	36.5	41.0	29.5	41.0
24-25	36.14059712013397	39.0	36.0	41.0	29.0	41.0
26-27	35.88735336175622	39.0	36.0	41.0	27.5	41.0
28-29	35.667505072362346	39.0	36.0	40.0	27.0	41.0
30-31	35.428125981213654	39.0	35.0	40.0	26.5	41.0
32-33	35.16700130387139	38.5	35.0	40.0	25.5	41.0
34-35	34.76281047334062	38.0	34.5	40.0	24.5	41.0
36-37	34.39986592444355	38.0	34.0	40.0	23.5	41.0
38-39	34.180646557539944	38.0	33.5	40.0	22.5	41.0
40-41	33.91850646198155	38.0	33.0	40.0	20.5	41.0
42-43	33.645103294608106	38.0	33.0	40.0	18.5	41.0
44-45	33.33894414159488	38.0	33.0	40.0	17.5	41.0
46-47	33.05798807222489	38.0	32.0	40.0	15.0	41.0
48-49	32.856831706016095	37.0	32.0	40.0	14.0	41.0
50-51	32.10286760283119	36.0	31.0	39.0	13.0	40.0
52-53	32.61273144032393	37.0	32.5	39.0	14.0	40.0
54-55	33.0641477296371	37.5	33.0	40.0	13.5	41.0
56-57	32.92295746781619	37.0	32.5	40.0	11.0	41.0
58-59	32.60349825919299	37.0	32.0	40.0	8.0	41.0
60-61	32.16288351661932	36.0	31.5	39.0	7.0	40.5
62-63	31.6864718929968	35.5	31.0	39.0	2.0	40.0
64-65	31.153122765670098	35.0	30.5	38.5	2.0	40.0
66-67	30.68172597137788	35.0	30.0	38.0	2.0	40.0
68-69	30.17638533182518	34.5	29.0	37.0	2.0	39.5
70-71	29.667773507199662	34.0	29.0	37.0	2.0	39.0
72-73	29.124584974206297	34.0	28.5	36.0	2.0	39.0
74-75	28.53855310627813	34.0	27.5	35.5	2.0	38.0
76-77	27.966052529371787	33.0	26.5	35.0	2.0	37.0
78-79	27.418024445697036	33.0	26.0	35.0	2.0	36.5
80-81	26.853281525252104	32.5	25.0	35.0	2.0	36.0
82-83	26.23903231684327	32.0	24.0	34.5	2.0	35.5
84-85	25.626352577415787	32.0	22.0	34.0	2.0	35.0
86-87	25.022002514428962	31.0	19.5	34.0	2.0	35.0
88-89	24.37706819348241	31.0	17.5	34.0	2.0	35.0
90-91	23.646746847191064	30.5	8.5	34.0	2.0	35.0
92-93	22.829689838756252	30.0	2.0	34.0	2.0	35.0
94-95	21.92695750942911	29.5	2.0	34.0	2.0	35.0
96-97	21.02146848199863	29.0	2.0	33.5	2.0	34.5
98	20.384485380625783	29.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80528.0
3	14536.0
4	11211.0
5	9645.0
6	9045.0
7	9595.0
8	10873.0
9	12517.0
10	14017.0
11	15402.0
12	17557.0
13	20341.0
14	17094.0
15	16948.0
16	18949.0
17	22278.0
18	24978.0
19	23999.0
20	24900.0
21	27140.0
22	29787.0
23	33218.0
24	37980.0
25	42634.0
26	48832.0
27	56104.0
28	66058.0
29	78113.0
30	93361.0
31	114544.0
32	144086.0
33	188738.0
34	257616.0
35	363647.0
36	490346.0
37	507413.0
38	209669.0
39	8393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06438064589695	17.54725766194935	13.572957974888824	24.815403717264882
2	16.100637777992954	18.875465654262573	38.56738554548612	26.45651102225836
3	19.769069246106284	22.139033486094853	28.269523934793572	29.822373333005288
4	13.974083143003227	15.020333595237654	34.602785984849085	36.402797276910036
5	15.615235745864805	35.669493650721044	32.99290704075179	15.722363562662359
6	35.1619995772964	33.1798036005514	16.4928219250679	15.165374897084291
7	30.883793513015036	30.52236118128449	19.434462478214716	19.159382827485764
8	27.84182295908602	33.55316906193648	19.083961041274694	19.521046937702806
9	26.976061110426237	16.048013353087192	19.08051862391821	37.89540691256837
10-11	26.535983549080346	24.94863817057298	26.24964686771665	22.26573141263003
12-13	26.506627125767363	22.079526139777666	26.73101521343037	24.6828315210246
14-15	23.874442614848732	23.71507598252311	24.178151999728843	28.23232940289931
16-17	24.270314871602785	26.725746474539058	24.878580243293538	24.125358410564623
18-19	24.228284884238988	26.03880374950775	26.26987945802874	23.463031908224526
20-21	24.843282874549473	25.274125808155866	26.145466242223304	23.737125075071354
22-23	24.69653379379855	24.88651671884452	26.014833215471654	24.402116271885273
24-25	24.386621439483143	25.132286357158574	26.083539897268782	24.3975523060895
26-27	24.363495300508276	25.34669784569096	26.055892865565934	24.233913988234825
28-29	24.42400260998078	25.417811478109858	26.0140494709993	24.14413644091006
30-31	24.580862674615254	25.329468914766984	25.807816095144563	24.2818523154732
32-33	24.454703231439066	25.31298401774681	25.724929782688598	24.507382968125526
34-35	24.63753070328626	25.305806392803614	25.88724802178337	24.16941488212676
36-37	24.62253149747678	25.26042757134754	25.783582610372818	24.333458320802865
38-39	24.56291624853555	25.348717387754583	25.789740687367924	24.298625676341942
40-41	24.718789065880987	25.279324516736327	25.775959560284033	24.22592685709865
42-43	24.695460368959527	25.173934349014843	25.67741683957088	24.453188442454753
44-45	24.608334332921718	25.32009936410333	25.626171479295213	24.445394823679738
46-47	24.723222268397834	25.25116637432381	25.590396049972963	24.43521530730539
48-49	24.64875072379047	25.323755139803446	25.528990828158875	24.49850330824721
50-51	24.555936680603814	25.52758637462724	25.468091150802408	24.448385793966533
52-53	24.686283831506184	25.470165923004394	25.41756670997869	24.42598353551073
54-55	24.637531590847047	25.320046074840224	25.491463377128525	24.550958957184196
56-57	24.5504014786088	25.46730626892293	25.533114121718697	24.449178130749573
58-59	24.623860245571482	25.382643018338253	25.504074243351976	24.489422492738285
60-61	24.725495502404232	25.268768819204446	25.56507523245342	24.440660445937905
62-63	24.59200444711508	25.425648368588256	25.530915563127994	24.451431621168673
64-65	24.633694292591915	25.26103560925109	25.508345848883994	24.596924249273002
66-67	24.638962541962435	25.395415938393	25.46981677229112	24.495804747353446
68-69	24.55678226140244	25.323959365316	25.50536646833584	24.61389190494572
70-71	24.499840988078542	25.222870095495846	25.58584173055652	24.691447185869098
72-73	24.65009410052644	25.269391953039204	25.415740638838457	24.664773307595897
74-75	24.520779329957858	25.43797057445686	25.397820798059133	24.64342929752615
76-77	24.719822033926487	25.226158884662336	25.41218150474692	24.641837576664262
78-79	24.592507773330045	25.25055313430104	25.493134231373695	24.66380486099522
80-81	24.56990934443573	25.29028684954419	25.50722815759483	24.632575648425252
82-83	24.72059771359979	25.23610669023062	25.39935990929145	24.64393568687814
84-85	24.689057886595712	25.253801300679722	25.309430284171654	24.747710528552915
86-87	24.64451997650661	25.16203949232744	25.523622414974977	24.669818116190967
88-89	24.631379588010386	25.0926489695509	25.468438859122735	24.80753258331598
90-91	24.70035192736756	25.08943844247599	25.424519907613526	24.78568972254292
92-93	24.655370694983432	25.17419865137345	25.45891995886233	24.711510694780785
94-95	24.719712232402923	25.075431641895406	25.42306004194025	24.781796083761424
96-97	24.760558229081646	25.009099840168968	25.437437510340725	24.792904420408657
98	24.703563820220733	25.070420216174867	25.32154554642795	24.90447041717645
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	623.0
1	470.0
2	511.0
3	705.0
4	564.0
5	520.0
6	864.5
7	1778.5
8	2570.5
9	2543.0
10	2016.0
11	1588.0
12	1697.5
13	1924.0
14	2151.0
15	2333.0
16	2442.5
17	2591.0
18	2632.0
19	2572.0
20	2415.5
21	2256.5
22	2145.5
23	2081.5
24	2236.5
25	2591.5
26	3134.5
27	4028.5
28	5493.5
29	7722.5
30	10763.5
31	14569.0
32	19056.0
33	24537.5
34	31095.0
35	38216.5
36	45972.5
37	54347.5
38	63202.5
39	71706.0
40	80158.0
41	89216.0
42	98471.5
43	108143.5
44	117121.5
45	125528.5
46	134131.5
47	140958.5
48	144005.0
49	144579.5
50	144689.0
51	144697.5
52	145536.0
53	145467.0
54	142996.5
55	138288.5
56	129531.5
57	119233.0
58	108953.5
59	96525.0
60	83065.5
61	69372.5
62	55966.5
63	43062.5
64	31532.5
65	23561.0
66	17984.0
67	13629.5
68	10300.0
69	7908.0
70	6061.0
71	4495.0
72	3400.0
73	2430.5
74	1790.5
75	1367.5
76	922.5
77	529.0
78	287.5
79	174.0
80	110.0
81	70.0
82	44.5
83	26.0
84	19.0
85	15.5
86	14.5
87	13.5
88	10.0
89	7.0
90	5.0
91	3.5
92	1.5
93	1.5
94	4.0
95	4.5
96	1.5
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47073035712709466
2	0.04895822693667145
3	0.0564611619082927
4	0.0543174662021152
5	0.06453154574331388
6	0.06374342232192509
7	0.04331526323952773
8	0.019955285029564087
9	0.012326250310520627
10-11	0.015005869943242505
12-13	0.011191352583720775
14-15	0.014958582537959175
16-17	0.014186221584998167
18-19	0.05367120499657639
20-21	0.08220127285085048
22-23	0.09845237779988728
24-25	0.06839335050811893
26-27	0.08365141994620585
28-29	0.08574782824710002
30-31	0.05676064880842044
32-33	0.03289627160876797
34-35	0.39776589077492075
36-37	0.623437151255386
38-39	0.5730287772233592
40-41	0.5663454906099823
42-43	0.5411570660623967
44-45	0.5241493626288267
46-47	0.5127688604239726
48-49	0.5032798544304516
50-51	0.5162681284149387
52-53	0.5990683750660447
54-55	0.5703806825274929
56-57	0.5262615333981485
58-59	0.5039103531675626
60-61	0.5171981140521775
62-63	0.5292406399309982
64-65	0.5469576544438182
66-67	0.5746838364082757
68-69	0.5282948918253317
70-71	0.47590044677140514
72-73	0.4591449428326795
74-75	0.43874830868713766
76-77	0.4543216274937801
78-79	0.44699207967486443
80-81	0.4443439849789981
82-83	0.46426774507170665
84-85	0.43083554953639425
86-87	0.43368855632182174
88-89	0.4605635649911793
90-91	0.44305146256792044
92-93	0.43870102128185434
94-95	0.44973474918129736
96-97	0.44354009908918157
98	0.43844882178700995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3172092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.31275445781949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.11984974176488	45.90997806841178
2	13.679510719328897	16.500979422359865
3	4.523100207040627	8.184018965260615
4	1.9273645383211497	4.649786566018884
5	1.0288731550144323	3.1027086983313747
6	0.629176009257338	2.2768402894253152
7	0.4287288362118491	1.810047191920237
8	0.3007193390835448	1.4509769327090876
9	0.2048896579821064	1.1121713669539257
>10	1.0966370896772115	11.573132341341772
>50	0.04942991583431902	1.9809536390749474
>100	0.011406152734110877	1.0751670603005403
>500	1.573188748284811E-4	0.0651216036056821
>1k	1.573188747252502E-4	0.3081178542860686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4271	0.13464300531006035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.260997474222059E-4
2	0.0	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
3	0.0	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
4	0.0	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
5	0.0	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
6	0.0	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
7	0.0	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
8	0.0	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
9	3.1524936855551475E-5	0.0	0.0	3.1524936855551475E-5	1.260997474222059E-4
10-11	3.1524936855551475E-5	0.0	0.0	4.728740528332721E-5	1.260997474222059E-4
12-13	3.1524936855551475E-5	0.0	0.0	6.304987371110295E-5	1.7338715270553314E-4
14-15	3.1524936855551475E-5	3.1524936855551475E-5	0.0	9.457481056665444E-5	1.8914962113330888E-4
16-17	3.1524936855551475E-5	6.304987371110295E-5	0.0	1.1033727899443016E-4	2.521994948444118E-4
18-19	3.1524936855551475E-5	6.304987371110295E-5	0.0	1.576246842777574E-4	2.8372443169996333E-4
20-21	3.1524936855551475E-5	6.304987371110295E-5	0.0	2.8372443169996333E-4	2.8372443169996333E-4
22-23	3.1524936855551475E-5	6.304987371110295E-5	0.0	3.467743054110663E-4	2.9948690012773907E-4
24-25	3.1524936855551475E-5	6.304987371110295E-5	0.0	4.5711158440549644E-4	3.152493685555148E-4
26-27	3.1524936855551475E-5	6.304987371110295E-5	0.0	6.620236739665811E-4	3.467743054110663E-4
28-29	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.001513196969066471	3.467743054110663E-4
30-31	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.005122802239027115	3.467743054110663E-4
32-33	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.01335081075832605	3.467743054110663E-4
34-35	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.023580652767952508	3.7829924226661776E-4
36-37	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.03980023278013374	3.7829924226661776E-4
38-39	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.06375918479035286	3.7829924226661776E-4
40-41	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.0908233430808438	3.7829924226661776E-4
42-43	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.11583838047572391	4.0982417912216923E-4
44-45	3.1524936855551475E-5	6.304987371110295E-5	0.0	0.14499894706710903	4.0982417912216923E-4
46-47	3.1524936855551475E-5	7.881234213887869E-5	0.0	0.17575152296969948	4.413491159777207E-4
48-49	3.1524936855551475E-5	9.457481056665444E-5	0.0	0.20450226538196242	4.728740528332722E-4
50-51	3.1524936855551475E-5	9.457481056665444E-5	0.0	0.23293775842566988	5.043989896888236E-4
52-53	3.1524936855551475E-5	9.457481056665444E-5	0.0	0.26039597842685525	5.989738002554781E-4
54-55	3.1524936855551475E-5	1.4186221584998164E-4	0.0	0.28976145710780143	6.304987371110296E-4
56-57	3.1524936855551475E-5	1.576246842777574E-4	0.0	0.3228626408061305	6.620236739665811E-4
58-59	3.1524936855551475E-5	1.576246842777574E-4	0.0	0.35378860386142646	6.935486108221326E-4
60-61	3.1524936855551475E-5	1.576246842777574E-4	0.0	0.3851874409695557	6.935486108221326E-4
62-63	3.1524936855551475E-5	1.576246842777574E-4	0.0	0.4172483017516516	7.88123421388787E-4
64-65	3.1524936855551475E-5	1.8914962113330888E-4	0.0	0.45138980836621384	8.354108266721142E-4
66-67	3.1524936855551475E-5	2.2067455798886035E-4	0.0	0.4879587351186535	8.826982319554414E-4
68-69	3.1524936855551475E-5	2.2067455798886035E-4	0.0	0.52433851224996	8.826982319554414E-4
70-71	3.1524936855551475E-5	2.2067455798886035E-4	0.0	0.5607813392549774	9.142231688109929E-4
72-73	3.1524936855551475E-5	2.2067455798886035E-4	0.0	0.5979807647445282	9.457481056665443E-4
74-75	3.1524936855551475E-5	2.2067455798886035E-4	0.0	0.636441187708301	9.930355109498716E-4
76-77	3.1524936855551475E-5	2.2067455798886035E-4	0.0	0.6769034441624013	0.0010087979793776472
78-79	3.1524936855551475E-5	2.3643702641663606E-4	0.0	0.7190207598014181	0.0010245604478054228
80-81	3.1524936855551475E-5	2.521994948444118E-4	0.0	0.7635654955783124	0.0010560853846609745
82-83	3.1524936855551475E-5	2.521994948444118E-4	0.0	0.8063763598281513	0.0010876103215165262
84-85	3.1524936855551475E-5	2.521994948444118E-4	0.0	0.8518195563054287	0.0011033727899443018
86	3.1524936855551475E-5	2.521994948444118E-4	0.0	0.8864812243781076	0.0011033727899443018
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2955	0.0	45.488018	1
GTATCAA	5410	0.0	39.65156	1
TCAACGC	6775	0.0	31.126358	4
TATCAAC	6930	0.0	30.496323	2
CAACGCA	6975	0.0	30.29957	5
ATCAACG	7020	0.0	30.040037	3
AACGCAG	7435	0.0	28.355686	6
GTACATG	9150	0.0	24.148588	1
ACGCAGA	8695	0.0	23.980442	7
TACATGG	9375	0.0	22.64068	2
CGCAGAG	9260	0.0	22.462116	8
ACATGGG	9745	0.0	20.84019	3
GAGTACA	7245	0.0	19.379078	1
GTACCTG	2950	0.0	19.349571	1
GCAGAGT	10985	0.0	18.851423	9
GTATAGG	575	0.0	18.4133	1
CATGGGG	7565	0.0	18.361725	4
AGAGTAC	9130	0.0	17.889492	10-11
AGTACAT	7005	0.0	17.539064	2
TACCTGG	3195	0.0	17.361782	2
>>END_MODULE