FastQCFastQC Report
Mon 15 Aug 2016
SRR2921445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172092
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.12770751920183904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.046.116931
GTATCAA50850.043.2695431
TCAACGC66400.032.1210864
TATCAAC67400.031.9963932
CAACGCA68150.031.2804535
ATCAACG68550.030.9057523
AACGCAG74600.028.8954946
ACGCAGA86400.024.5648277
GTACATG94000.024.065621
CGCAGAG92550.023.0350888
TACATGG96050.022.9964432
ACATGGG100000.020.8058783
GTACCTG29200.019.8979471
GAGTACA72600.019.417191
GCAGAGT109200.019.3048579
AGTACAT70300.019.2572652
CATGGGG77200.018.9515784
AGAGTAC89900.018.03573610-11
TACCTGG32950.016.8669092
GTATAGG7100.016.7692221
Produced by FastQC (version 0.11.5)