##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3172092
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03615216708721	33.0	31.0	34.0	30.0	34.0
2	32.15749858453034	34.0	31.0	34.0	30.0	34.0
3	32.27565720035863	34.0	31.0	34.0	30.0	34.0
4	35.81231250543805	37.0	35.0	37.0	35.0	37.0
5	35.633757785083155	37.0	35.0	37.0	35.0	37.0
6	35.66839202646077	37.0	35.0	37.0	35.0	37.0
7	35.603085913018916	37.0	35.0	37.0	33.0	37.0
8	35.55917735046777	37.0	35.0	37.0	33.0	37.0
9	37.195560532292255	39.0	37.0	39.0	34.0	39.0
10-11	37.11330929241649	39.0	37.0	39.0	34.0	39.0
12-13	37.00810080539909	39.0	37.0	39.0	33.0	39.0
14-15	38.34012065223833	40.0	38.0	41.0	34.0	41.0
16-17	38.249046843534174	40.0	38.0	41.0	33.5	41.0
18-19	38.148254212046815	40.0	38.0	41.0	33.5	41.0
20-21	38.04411773050718	40.0	38.0	41.0	33.0	41.0
22-23	38.013165128880246	40.0	38.0	41.0	33.5	41.0
24-25	38.07230922053963	40.0	38.0	41.0	33.0	41.0
26-27	37.97850755904936	40.0	38.0	41.0	33.0	41.0
28-29	37.855016815401314	40.0	38.0	41.0	33.0	41.0
30-31	37.72319939018162	40.0	38.0	41.0	33.0	41.0
32-33	37.5728468783377	40.0	38.0	41.0	32.5	41.0
34-35	37.4465836741179	40.0	37.0	41.0	32.0	41.0
36-37	37.36595108212498	40.0	37.0	41.0	32.0	41.0
38-39	37.34606436383308	40.0	37.0	41.0	32.0	41.0
40-41	37.19593662478894	40.0	37.0	41.0	31.5	41.0
42-43	36.977539743487895	40.0	37.0	41.0	31.0	41.0
44-45	36.79096224195263	40.0	36.0	41.0	31.0	41.0
46-47	36.543164574041356	39.0	36.0	41.0	30.0	41.0
48-49	36.2393187208946	39.0	35.0	40.0	30.0	41.0
50-51	35.9825194225136	39.0	35.0	40.0	29.0	41.0
52-53	35.69045128577608	38.5	35.0	40.0	28.5	41.0
54-55	35.383360886128145	38.0	34.5	40.0	28.0	41.0
56-57	35.03087725702785	38.0	34.0	40.0	27.5	41.0
58-59	34.66547502405352	37.5	34.0	40.0	26.5	41.0
60-61	34.263717288149266	37.0	33.5	39.5	26.0	41.0
62-63	33.84065626091551	36.0	33.0	39.0	26.0	40.5
64-65	33.35494793341429	36.0	32.5	39.0	25.0	40.0
66-67	32.845936214964766	35.0	32.0	38.5	23.5	40.0
68-69	32.36613881312395	35.0	31.5	38.0	22.0	40.0
70-71	31.840963629049853	35.0	31.0	37.0	21.5	39.0
72-73	31.314158290490944	34.0	30.5	36.5	20.0	39.0
74-75	30.806731015367777	34.0	30.0	36.0	18.0	38.5
76-77	29.462378770855324	32.5	28.5	34.5	18.0	36.0
78-79	30.40665340097324	34.0	30.0	35.0	18.0	37.0
80-81	30.46102020370153	34.0	31.0	35.0	18.0	37.0
82-83	30.15193332980254	34.0	30.0	35.0	17.5	36.0
84-85	29.69256219554792	34.0	30.0	35.0	11.0	36.0
86-87	29.209539004543373	34.0	29.0	35.0	7.0	35.0
88-89	28.70034617533161	33.5	29.0	35.0	2.0	35.0
90-91	28.278360621318676	33.0	28.5	35.0	2.0	35.0
92-93	27.888591030777164	33.0	27.0	35.0	2.0	35.0
94-95	27.447821816012905	33.0	27.0	34.5	2.0	35.0
96-97	26.74560037981244	32.5	25.5	34.0	2.0	35.0
98-99	25.807982082486888	32.0	24.5	34.0	2.0	35.0
100-101	22.198626017152087	28.5	9.5	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16356.0
3	8954.0
4	5318.0
5	4179.0
6	3562.0
7	3604.0
8	4244.0
9	5076.0
10	6066.0
11	7242.0
12	8624.0
13	9935.0
14	11369.0
15	12458.0
16	14490.0
17	17003.0
18	17242.0
19	15163.0
20	15327.0
21	16176.0
22	17541.0
23	19815.0
24	22439.0
25	27006.0
26	32508.0
27	39261.0
28	48149.0
29	59036.0
30	72882.0
31	91892.0
32	119610.0
33	161926.0
34	233052.0
35	359899.0
36	576019.0
37	739669.0
38	338265.0
39	10735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54521918391729	17.888606635838435	13.905054402667972	24.661119777576303
2	16.25179849764761	19.14660734934548	38.164057032393764	26.437537120613147
3	19.71621488271882	22.303396663269133	28.545099210033587	29.435289243978463
4	14.19779294960198	15.305229568822702	34.59285269970008	35.90412478187523
5	15.641921116544644	35.71151600594186	33.0560277320313	15.590535145482196
6	35.237492559084885	33.27369034373479	16.42600741394327	15.062809683237049
7	30.98255395377041	30.530864844093593	19.492481523243203	18.994099678892802
8	27.8797692662609	33.592348092609086	19.105057825450015	19.42282481568
9	26.901232140868032	16.009816231239963	19.1668636245017	37.9220880033903
10-11	26.59757361746844	24.959094832142863	26.312523371564083	22.130808178824612
12-13	26.508447095462362	22.0446841748925	26.83504003481302	24.61182869483212
14-15	23.841208036097488	23.691064730392686	24.246684867671522	28.221042365838304
16-17	24.238594448537203	26.71959991321109	25.006953835363454	24.034851802888255
18-19	24.25343038290259	26.02913852396833	26.328824456895134	23.388606636233945
20-21	24.83134049070662	25.21428918302387	26.211393732683902	23.74297659358561
22-23	24.668618262177997	24.885160841053143	26.121938765694686	24.324282131074177
24-25	24.397321069566477	25.100797327755224	26.19683522408149	24.305046378596813
26-27	24.301784842648544	25.360049215292307	26.132112536782188	24.206053405276958
28-29	24.395699860545818	25.35297516706516	26.122188350648827	24.12913662174019
30-31	24.577957810440488	25.264949714960544	25.914205377840034	24.24288709675894
32-33	24.408836532286564	25.26230933096421	25.84127987974487	24.487574257004347
34-35	24.56900132525245	25.282071322132822	25.98792155426087	24.161005798353862
36-37	24.575098096121035	25.27869765743736	25.915252455142042	24.230951791299564
38-39	24.52928793612192	25.326171658540652	25.91213690105434	24.232403504283084
40-41	24.701305703664232	25.19640734422839	25.89704458815628	24.2052423639511
42-43	24.627930496584476	25.19263944251996	25.77880181135616	24.400628249539405
44-45	24.556720845667883	25.25252652643879	25.783276007701993	24.40747662019134
46-47	24.580201415029265	25.266699513214164	25.7837409589878	24.369358112768765
48-49	24.556172724495596	25.345325095026467	25.66181201767585	24.436690162802083
50-51	24.553035255424525	25.45037756160292	25.602669595331328	24.39391758764122
52-53	24.688833634876485	25.411433797485955	25.499264348204942	24.400468219432618
54-55	24.684401799837797	25.253719909109613	25.558626744883473	24.503251546169118
56-57	24.558729878498603	25.421424473042077	25.590687835358356	24.429157813100964
58-59	24.600199839000194	25.376290153996457	25.55478651290087	24.46872349410248
60-61	24.7210051192936	25.31436775557533	25.589752837554013	24.374874287577057
62-63	24.532571490549905	25.41562513400143	25.58810861871959	24.46369475672908
64-65	24.68082188433749	25.23987915598352	25.52952016286986	24.549778796809125
66-67	24.634081997233213	25.377137475040023	25.486951115111474	24.50182941261529
68-69	24.545894167836984	25.389138935470317	25.51788979191666	24.547077104776037
70-71	24.537225772908865	25.21375848247321	25.627276588137484	24.621739156480444
72-73	24.739961062946556	25.269113317169218	25.40884773347844	24.58207788640579
74-75	24.53527283554578	25.45632926454426	25.36345558835687	24.6449423115531
76-77	24.666877788864415	25.27420457235027	25.44658589951416	24.61233173927115
78-79	24.524185736385245	25.292984416281346	25.51177135235092	24.671058494982493
80-81	24.553147245468402	25.375597877143914	25.49485006516849	24.576404812219195
82-83	24.665566340878577	25.295820296659098	25.413788927913945	24.62482443454838
84-85	24.636957769962947	25.333056069076004	25.3494365218828	24.680549639078244
86-87	24.592133229219073	25.310259652294565	25.474558392886564	24.6230487255998
88-89	24.558338239057594	25.21491033815032	25.437169358806656	24.789582063985435
90-91	24.579867313311585	25.240791157756306	25.479471254610992	24.699870274321118
92-93	24.635796743215927	25.25975768880551	25.505030513623655	24.599415054354907
94-95	24.646223701065214	25.225065884283897	25.429047851899632	24.699662562751254
96-97	24.638668766974163	25.127631827634804	25.516355805709264	24.717343599681772
98-99	24.50649424146727	25.21376461072306	25.440514866849647	24.839226280960027
100-101	25.037698712332425	24.760306180066642	25.539849028598056	24.66214607900288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	308.0
1	273.5
2	370.5
3	523.0
4	443.5
5	389.5
6	681.5
7	1540.0
8	2289.0
9	2212.0
10	1581.5
11	1045.5
12	891.5
13	903.0
14	988.5
15	1006.5
16	1011.0
17	1078.5
18	1128.0
19	1223.0
20	1322.5
21	1360.0
22	1434.5
23	1519.0
24	1671.0
25	1998.0
26	2464.5
27	3093.5
28	4102.0
29	5748.5
30	8348.0
31	11627.0
32	15552.0
33	20416.0
34	25978.5
35	32405.0
36	39862.5
37	48019.0
38	56636.0
39	64876.5
40	73266.0
41	81894.0
42	90681.0
43	99936.0
44	109519.0
45	118946.5
46	126960.5
47	133637.0
48	139878.0
49	143870.0
50	143865.0
51	144051.0
52	144277.5
53	143553.0
54	142180.5
55	137433.0
56	129382.5
57	118623.0
58	107987.0
59	96617.0
60	83071.5
61	69810.0
62	56454.0
63	42883.0
64	31385.5
65	23289.0
66	17719.0
67	13670.5
68	10454.0
69	7901.5
70	5928.5
71	4406.5
72	3177.0
73	2373.5
74	1794.0
75	1239.0
76	797.5
77	473.5
78	260.5
79	130.0
80	65.0
81	35.5
82	23.0
83	11.0
84	5.0
85	3.5
86	2.5
87	1.5
88	1.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31017385372177103
2	0.0
3	0.0058321133182770235
4	0.05951908078328119
5	6.304987371110295E-4
6	0.06680134119691358
7	0.0767316963064123
8	0.10775223417227495
9	0.09630868209370977
10-11	0.10015472439008705
12-13	0.09969761280568155
14-15	0.06684862860219691
16-17	0.03719942548955074
18-19	0.040115482148689256
20-21	0.028687692538551846
22-23	0.04265323956556115
24-25	0.035465553962495414
26-27	0.022697954535997066
28-29	0.025204187016013405
30-31	0.002364370264166361
32-33	0.0053277143285882
34-35	0.007676322124326784
36-37	0.010418991630759763
38-39	0.015368406717081347
40-41	0.019608510724153018
42-43	0.0032785934329773534
44-45	0.014028596900720407
46-47	0.024573688278902377
48-49	0.0025535198852996697
50-51	0.007108873260926859
52-53	0.002364370264166361
54-55	0.004397728691349432
56-57	0.0035307929278217656
58-59	0.001213710068938732
60-61	0.005359239265443751
62-63	0.015258069438086917
64-65	0.007392597692626821
66-67	0.039894807590700396
68-69	0.06355427270079178
70-71	0.01276759942649835
72-73	0.03393659452500117
74-75	0.05222105790122102
76-77	0.3034748046399663
78-79	0.07381563964727379
80-81	0.034078456740851144
82-83	0.028687692538551846
84-85	0.019829185282141882
86-87	0.11910121144027348
88-89	0.5284998039148928
90-91	0.13409131891508821
92-93	0.04857992769440483
94-95	0.007660559655899009
96-97	0.005532626418149284
98-99	0.010103742262204249
100-101	0.0073453102873434935
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3172092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.65600989118105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72663020679228	41.20400680348289
2	15.481262198740275	17.542130885195924
3	5.135960119466512	8.729490219876183
4	2.2909175093347454	5.191769802749968
5	1.2091085678027866	3.425163348847321
6	0.724798582079388	2.4638517381242284
7	0.5039983239292927	1.9988173817973673
8	0.31577579953681173	1.4312477457562567
9	0.2637253796870695	1.3447464948095476
>10	1.2723458004686186	12.725249976894476
>50	0.06019998690075438	2.2997472084699644
>100	0.014831824807185198	1.2544301438376415
>500	2.7856278485008476E-4	0.09308374515430844
>1k	1.6713766942396297E-4	0.29626450500385715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4051	0.12770751920183904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.304987371110295E-5	0.0
5	0.0	0.0	0.0	6.304987371110295E-5	0.0
6	0.0	0.0	0.0	6.304987371110295E-5	3.1524936855551475E-5
7	0.0	0.0	0.0	6.304987371110295E-5	3.1524936855551475E-5
8	0.0	0.0	0.0	6.304987371110295E-5	3.1524936855551475E-5
9	0.0	0.0	0.0	6.304987371110295E-5	6.304987371110295E-5
10-11	0.0	0.0	0.0	7.881234213887869E-5	7.881234213887869E-5
12-13	0.0	0.0	0.0	1.260997474222059E-4	9.457481056665444E-5
14-15	0.0	0.0	0.0	1.260997474222059E-4	1.2609974742220593E-4
16-17	0.0	0.0	0.0	1.260997474222059E-4	1.8914962113330888E-4
18-19	0.0	3.1524936855551475E-5	0.0	1.576246842777574E-4	2.2067455798886035E-4
20-21	0.0	3.1524936855551475E-5	0.0	1.8914962113330888E-4	2.2067455798886035E-4
22-23	0.0	3.1524936855551475E-5	0.0	2.2067455798886033E-4	2.2067455798886035E-4
24-25	0.0	3.1524936855551475E-5	0.0	3.62536773838842E-4	2.2067455798886035E-4
26-27	0.0	3.1524936855551475E-5	0.0	5.832113318277024E-4	2.3643702641663606E-4
28-29	0.0	3.1524936855551475E-5	0.0	0.001465909563783144	2.8372443169996333E-4
30-31	0.0	3.1524936855551475E-5	0.0	0.005233139518021545	2.8372443169996333E-4
32-33	0.0	3.1524936855551475E-5	0.0	0.013413860632037153	2.8372443169996333E-4
34-35	0.0	3.1524936855551475E-5	0.0	0.023627940173235832	2.8372443169996333E-4
36-37	0.0	4.728740528332721E-5	0.0	0.03998938240126705	2.8372443169996333E-4
38-39	0.0	6.304987371110295E-5	0.0	0.06424782131161391	3.152493685555148E-4
40-41	0.0	6.304987371110295E-5	0.0	0.09139079194424374	3.152493685555148E-4
42-43	0.0	6.304987371110295E-5	0.0	0.11673684117610712	3.152493685555148E-4
44-45	0.0	6.304987371110295E-5	0.0	0.14597622010963113	3.467743054110663E-4
46-47	0.0	6.304987371110295E-5	0.0	0.1767130335437938	3.7829924226661776E-4
48-49	0.0	6.304987371110295E-5	0.0	0.2054322510192012	3.7829924226661776E-4
50-51	0.0	6.304987371110295E-5	0.0	0.23399384381033084	4.0982417912216923E-4
52-53	0.0	6.304987371110295E-5	0.0	0.261325964064094	4.413491159777207E-4
54-55	0.0	6.304987371110295E-5	0.0	0.29058110546604576	4.5711158440549644E-4
56-57	0.0	6.304987371110295E-5	0.0	0.3233039899221082	5.043989896888236E-4
58-59	0.0	6.304987371110295E-5	0.0	0.3540723282931264	5.043989896888236E-4
60-61	0.0	6.304987371110295E-5	0.0	0.38485642913257245	5.043989896888236E-4
62-63	0.0	6.304987371110295E-5	0.0	0.4166650904198239	5.359239265443752E-4
64-65	0.0	6.304987371110295E-5	0.0	0.4505859224763973	5.674488633999267E-4
66-67	0.0	6.304987371110295E-5	0.0	0.48690264973399255	5.832113318277024E-4
68-69	0.0	9.457481056665444E-5	0.0	0.5231720895863046	6.147362686832539E-4
70-71	0.0	9.457481056665444E-5	0.0	0.5592523798174832	6.462612055388053E-4
72-73	0.0	9.457481056665444E-5	0.0	0.5959946937226285	6.935486108221326E-4
74-75	0.0	9.457481056665444E-5	0.0	0.6339507176967125	7.723609529610112E-4
76-77	0.0	9.457481056665444E-5	0.0	0.6741765371243962	7.88123421388787E-4
78-79	0.0	9.457481056665444E-5	0.0	0.7153323421893186	7.88123421388787E-4
80-81	0.0	9.457481056665444E-5	0.0	0.7592938666343851	9.142231688109929E-4
82-83	0.0	9.457481056665444E-5	0.0	0.8013796573365464	9.457481056665444E-4
84-85	0.0	9.457481056665444E-5	0.0	0.8459401555818684	9.772730425220957E-4
86-87	0.0	9.457481056665444E-5	0.0	0.8916355515539902	0.0010087979793776472
88-89	0.0	9.457481056665444E-5	0.0	0.938891431900462	0.0010718478530887502
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2685	0.0	46.11693	1
GTATCAA	5085	0.0	43.269543	1
TCAACGC	6640	0.0	32.121086	4
TATCAAC	6740	0.0	31.996393	2
CAACGCA	6815	0.0	31.280453	5
ATCAACG	6855	0.0	30.905752	3
AACGCAG	7460	0.0	28.895494	6
ACGCAGA	8640	0.0	24.564827	7
GTACATG	9400	0.0	24.06562	1
CGCAGAG	9255	0.0	23.035088	8
TACATGG	9605	0.0	22.996443	2
ACATGGG	10000	0.0	20.805878	3
GTACCTG	2920	0.0	19.897947	1
GAGTACA	7260	0.0	19.41719	1
GCAGAGT	10920	0.0	19.304857	9
AGTACAT	7030	0.0	19.257265	2
CATGGGG	7720	0.0	18.951578	4
AGAGTAC	8990	0.0	18.035736	10-11
TACCTGG	3295	0.0	16.866909	2
GTATAGG	710	0.0	16.769222	1
>>END_MODULE