FastQCFastQC Report
Mon 15 Aug 2016
SRR2921444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5348041
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT164300.30721529621781135No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72610.13576934058658113No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63150.11808062054872055No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC60530.1131816304325266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG202100.025.3524271
TACATGG205600.024.222992
GTACCTG55350.023.371111
ACATGGG209750.022.9129163
TACCTGG57900.021.7808692
AGTACAT141100.021.2880212
GTATAGG10900.021.1170981
GAGTACA156900.021.066491
CATGGGG161600.020.1480924
GTGTAGC28400.017.3442751
GTATACG3201.2210876E-817.2632261
AGAGTAC159350.016.27632310-11
ATGGGGG81450.015.5394275
TACCGTA6300.015.2776317
ACCTGGG80550.015.143883
GTACCGT9800.014.9706036
TAGACAG24700.014.2958765
TATATCG3851.243261E-714.2934655
GTATCAA290050.014.220861
GTCTTAG21450.014.1646981
Produced by FastQC (version 0.11.5)