##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921444_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5348041
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.71253567427774	31.0	31.0	34.0	26.0	34.0
2	29.787281735499036	31.0	30.0	34.0	26.0	34.0
3	29.919838684856753	31.0	31.0	34.0	26.0	34.0
4	33.40748266514785	37.0	35.0	37.0	28.0	37.0
5	33.366884434879985	37.0	35.0	37.0	28.0	37.0
6	33.46873817908277	37.0	35.0	37.0	28.0	37.0
7	33.32875196730915	37.0	35.0	37.0	27.0	37.0
8	33.29824397382144	37.0	35.0	37.0	27.0	37.0
9	34.65534089959296	38.0	35.0	39.0	27.0	39.0
10-11	34.5021405034105	38.0	35.0	39.0	27.0	39.0
12-13	34.31056025935478	38.0	35.0	39.0	27.0	39.0
14-15	35.19808589724724	39.0	35.5	40.0	26.0	41.0
16-17	35.028553633003185	39.0	35.0	40.0	25.0	41.0
18-19	34.81609724009221	39.0	34.5	40.0	24.5	41.0
20-21	34.67813204872588	39.0	34.0	40.0	24.0	41.0
22-23	34.70074004668251	39.0	34.5	40.0	23.5	41.0
24-25	34.53082998054802	39.0	34.0	40.0	22.0	41.0
26-27	34.216163731729054	38.0	34.0	40.0	18.5	41.0
28-29	33.94417516619637	38.0	33.5	40.0	17.5	41.0
30-31	33.65787285475186	38.0	33.0	40.0	16.0	41.0
32-33	33.3503268767012	38.0	33.0	40.0	15.5	41.0
34-35	32.89361740121289	38.0	32.0	40.0	9.0	41.0
36-37	32.47258977633118	38.0	31.0	40.0	8.5	41.0
38-39	32.19122375838181	37.0	31.0	40.0	8.0	41.0
40-41	31.89305579370091	37.0	30.0	40.0	4.5	41.0
42-43	31.55227671216432	37.0	30.0	40.0	2.0	40.5
44-45	31.192394373939916	36.5	30.0	39.0	2.0	40.0
46-47	30.85641807906858	36.0	29.0	39.0	2.0	40.0
48-49	30.601281198106	36.0	28.5	39.0	2.0	40.0
50-51	29.901173158545344	35.0	28.0	38.5	2.0	39.5
52-53	30.504386279013193	35.5	29.5	38.5	2.0	39.5
54-55	30.91288043603256	36.0	30.0	39.0	2.0	40.0
56-57	30.70587585248505	36.0	29.5	39.0	2.0	40.0
58-59	30.329769723156573	35.0	29.0	39.0	2.0	40.0
60-61	29.805838530407677	35.0	28.0	38.5	2.0	40.0
62-63	29.281941929764564	34.5	27.5	38.0	2.0	40.0
64-65	28.68552148347404	34.0	26.0	37.5	2.0	40.0
66-67	28.138755948206082	34.0	26.0	37.0	2.0	39.0
68-69	27.57002264941499	33.5	25.0	36.0	2.0	39.0
70-71	26.992755665111765	33.0	23.5	36.0	2.0	39.0
72-73	26.397864002912467	32.5	22.0	35.0	2.0	37.5
74-75	25.754381744642572	32.0	20.0	35.0	2.0	37.0
76-77	25.13526392187345	31.5	18.0	35.0	2.0	36.5
78-79	24.516522218135577	31.0	13.0	34.0	2.0	36.0
80-81	23.86658180070048	31.0	7.0	34.0	2.0	35.0
82-83	23.187558584535907	30.0	2.0	34.0	2.0	35.0
84-85	22.505838586502982	29.5	2.0	34.0	2.0	35.0
86-87	21.827957751258825	29.0	2.0	34.0	2.0	35.0
88-89	21.101749687408905	29.0	2.0	34.0	2.0	35.0
90-91	20.286422261908612	27.5	2.0	33.0	2.0	34.5
92-93	19.390841337977776	26.0	2.0	33.0	2.0	34.0
94-95	18.42033110815717	25.0	2.0	32.0	2.0	34.0
96-97	17.48717240574633	23.5	2.0	32.0	2.0	34.0
98	16.853546373335583	19.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	228703.0
3	38885.0
4	29372.0
5	24957.0
6	23502.0
7	24624.0
8	28139.0
9	32280.0
10	35855.0
11	38896.0
12	41386.0
13	43729.0
14	38495.0
15	39733.0
16	43449.0
17	49395.0
18	55306.0
19	56592.0
20	60483.0
21	65876.0
22	71881.0
23	78652.0
24	87152.0
25	97644.0
26	109288.0
27	123910.0
28	141007.0
29	162324.0
30	189903.0
31	225527.0
32	273726.0
33	342320.0
34	437584.0
35	564913.0
36	675105.0
37	577439.0
38	184859.0
39	5150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76813723888225	16.542481840880395	12.947301083593366	24.742079836643992
2	16.390435733890445	18.328666608800283	38.10989799552832	27.170999661780947
3	21.645405201941976	21.132559553085503	26.561337465831958	30.660697779140563
4	14.084298885861354	13.731246673532402	33.65813687882164	38.52631756178461
5	16.141060656576258	35.37568116615571	31.952534744478733	16.530723432789298
6	36.67680753492706	31.82681567381579	15.787947334841364	15.708429456415782
7	32.70058535999556	29.424417516018224	18.08020327946006	19.794793844526158
8	28.877763418981168	32.57792556325029	18.01759461688507	20.526716400883473
9	27.074831611202804	16.498380722311172	18.380606367974544	38.04618129851148
10-11	27.714866567414216	24.200078656415275	25.034984899875766	23.05006987629474
12-13	27.856971014605225	21.139163349776208	25.741946685962898	25.261918949655666
14-15	24.576371268957644	22.84863386215335	23.576742018025044	28.998252850863963
16-17	25.12069615537676	25.89010141918033	24.088485953316358	24.900716472126554
18-19	25.052372121521095	25.483855729362766	25.211235392749987	24.25253675636615
20-21	25.81205362197202	24.319025844428165	25.043130187762983	24.825790345836833
22-23	25.41276226832364	24.043338961980616	25.0243406865103	25.519558083185444
24-25	25.45884934188941	24.20186742137162	25.139507432834023	25.199775803904945
26-27	25.348352677500824	24.258823941112563	25.404942823648057	24.987880557738556
28-29	25.04978330391217	24.898220488994525	25.147834096449646	24.904162110643664
30-31	25.301036781826813	24.513787525766137	24.93883290297789	25.24634278942916
32-33	25.233678247301857	24.54984182039161	24.927054455158505	25.28942547714803
34-35	25.513342995288763	24.41870613128773	25.124246590819943	24.943704282603562
36-37	25.473034487992553	24.32949105221626	25.168177971429422	25.029296488361762
38-39	25.315628526925256	24.560394777817162	25.134704046822293	24.989272648435293
40-41	25.39128716768706	24.607514431796957	25.010524776296222	24.99067362421976
42-43	25.280980332809712	24.56516139871905	24.77299010509083	25.380868163380406
44-45	25.39594520764169	24.336824837423563	24.844150701064645	25.4230792538701
46-47	25.643483628807722	24.578917172505932	24.91665900231678	24.86094019636957
48-49	25.545100141176746	24.56827617924947	24.751564531223977	25.135059148349807
50-51	24.95824386933192	24.85802727672613	25.112096585232578	25.071632268709365
52-53	25.32663731422839	24.921261882175976	24.675934244445504	25.076166559150124
54-55	25.181239401441648	24.518647681995827	24.934398325229214	25.36571459133331
56-57	25.35489591420468	24.556329018242742	24.932203133439963	25.156571934112616
58-59	25.69691387011695	24.474919799212227	24.74417989475147	25.083986435919357
60-61	25.41983218174442	24.512712823795717	25.162187924353454	24.905267070106408
62-63	25.123744421635298	24.723628062416047	25.040552841592856	25.112074674355796
64-65	25.125730760855884	24.823911150231	24.907861660310466	25.142496428602644
66-67	25.348897194778758	24.596234675792992	24.65636683682649	25.39850129260176
68-69	25.08667190954439	24.658522354108474	24.89550418537613	25.359301550971015
70-71	25.301339803487476	24.45636749904539	24.944183463279227	25.298109234187915
72-73	25.40361619604675	24.343149799282273	25.029091464616393	25.22414254005458
74-75	25.012528170781923	25.032956270910407	24.793339162601857	25.16117639570582
76-77	25.27558031176867	24.475854476657254	24.67688682013328	25.5716783914408
78-79	25.22604906133319	24.487694577010092	24.964959074245453	25.321297287411266
80-81	25.34987611502812	24.550432886228336	24.873103690561994	25.226587308181553
82-83	25.457717098352767	24.36855614197949	24.95394611681582	25.219780642851923
84-85	25.302661744939105	24.637687825633193	24.78136702506989	25.278283404357822
86-87	25.261942763203965	24.649439691160342	24.915226615904334	25.173390929731355
88-89	25.09024094487213	24.527508534884433	24.69995273160434	25.682297788639097
90-91	25.283009515000565	24.26363167543492	24.798223198110087	25.655135611454426
92-93	25.50775661629085	24.59058639018408	24.823650537837814	25.07800645568725
94-95	25.551395477747768	24.342877122246826	24.81316266227621	25.2925647377292
96-97	25.16384503706886	24.305489315711323	25.16097220980877	25.369693437411044
98	25.376654066313048	24.68698607464719	24.531166770965527	25.405193088074235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	486.0
1	352.5
2	282.0
3	351.0
4	345.5
5	371.5
6	615.5
7	1266.0
8	1782.0
9	1810.0
10	1599.0
11	1518.0
12	1792.0
13	2183.5
14	2524.0
15	2971.0
16	3444.5
17	3649.0
18	3687.5
19	3490.0
20	3222.5
21	2971.0
22	2659.0
23	2529.0
24	2610.0
25	2792.5
26	3147.0
27	3798.0
28	4806.5
29	6254.0
30	8356.0
31	11438.0
32	15767.5
33	21266.0
34	28354.5
35	37914.5
36	49292.0
37	62413.0
38	76574.0
39	91520.5
40	107727.5
41	124059.5
42	141474.5
43	161007.5
44	180153.0
45	201248.0
46	233300.5
47	265426.5
48	275122.0
49	266305.5
50	260049.0
51	259862.5
52	262093.5
53	265728.0
54	263865.0
55	254176.5
56	239330.5
57	223083.0
58	205303.5
59	186010.0
60	165480.5
61	140602.0
62	113585.0
63	87353.5
64	64273.5
65	47965.5
66	36468.5
67	27505.0
68	20570.0
69	15520.0
70	11639.5
71	8502.5
72	6350.0
73	4585.5
74	3450.0
75	2574.5
76	1645.5
77	1018.0
78	637.0
79	371.0
80	210.0
81	119.5
82	93.5
83	65.5
84	40.0
85	30.5
86	21.5
87	18.0
88	18.5
89	16.5
90	11.0
91	13.5
92	12.0
93	7.5
94	9.0
95	6.5
96	4.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.44350819300001626
2	0.04474535629027526
3	0.051757269624522324
4	0.0496630448420272
5	0.05914315166992923
6	0.062284488843671916
7	0.039266714671783554
8	0.017782212215650555
9	0.011125569157005341
10-11	0.013724651699566253
12-13	0.010050409112420791
14-15	0.014182763370737062
16-17	0.013631159521776293
18-19	0.053823446753680455
20-21	0.08107641657945405
22-23	0.0960164665902898
24-25	0.06754809845324672
26-27	0.0814223376372769
28-29	0.08193654461512169
30-31	0.05323444603360371
32-33	0.029674417230533573
34-35	0.37260372536410996
36-37	0.5848403181651001
38-39	0.5365422591188063
40-41	0.5319798408426563
42-43	0.506213396643743
44-45	0.49202128405522694
46-47	0.48205501790281713
48-49	0.472705800123821
50-51	0.48743081812573985
52-53	0.565711818589274
54-55	0.5352707655008628
56-57	0.4944894775488819
58-59	0.4731919594483288
60-61	0.48567316518328857
62-63	0.4971727030514538
64-65	0.5169089017829145
66-67	0.5408335500793655
68-69	0.4961816859668802
70-71	0.44693000670712885
72-73	0.43054082794054865
74-75	0.4121883134403793
76-77	0.4259223143577246
78-79	0.420387577432559
80-81	0.4176202089699761
82-83	0.4356735485012176
84-85	0.4037272713503879
86-87	0.4072893233241854
88-89	0.4324948144563589
90-91	0.41683487467654046
92-93	0.4121041704803684
94-95	0.42170581713939737
96-97	0.4165450489253915
98	0.4113655822758277
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5348041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.91129672256857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8124267812685	36.8407393489276
2	13.361542319117573	13.337838067212685
3	5.0324740099102785	7.535319106717392
4	2.376806696847691	4.745180171942128
5	1.3903649167660448	3.4697457956679707
6	0.8574575608982945	2.5678091249402812
7	0.5670223826943175	1.9810575673695734
8	0.39755502738146736	1.5873989548148237
9	0.3148581183408131	1.4143479273016142
>10	1.7070820633752375	15.63910607344497
>50	0.12762318692531813	4.376410046673847
>100	0.05260128683686041	4.427759538381316
>500	0.001168194321553055	0.37443772604258
>1k	8.290376649453973E-4	0.9297714096618621
>5k	1.5073412089910846E-4	0.46405813793305206
>10k+	3.7683530224777114E-5	0.3090210029684288
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	16430	0.30721529621781135	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	7261	0.13576934058658113	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	6315	0.11808062054872055	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	6053	0.1131816304325266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.349217778996085E-5
2	0.0	0.0	0.0	0.0	9.349217778996085E-5
3	0.0	0.0	0.0	0.0	9.349217778996085E-5
4	0.0	0.0	0.0	1.869843555799217E-5	9.349217778996085E-5
5	0.0	0.0	0.0	1.869843555799217E-5	1.3088904890594518E-4
6	0.0	0.0	0.0	1.869843555799217E-5	2.430796622538982E-4
7	0.0	0.0	0.0	1.869843555799217E-5	2.430796622538982E-4
8	0.0	0.0	0.0	1.869843555799217E-5	2.430796622538982E-4
9	0.0	0.0	0.0	3.739687111598434E-5	2.430796622538982E-4
10-11	0.0	0.0	0.0	6.544452445297259E-5	2.430796622538982E-4
12-13	0.0	3.739687111598434E-5	0.0	2.991749689278747E-4	4.768101067288003E-4
14-15	0.0	3.739687111598434E-5	0.0	3.3657184004385905E-4	5.796515022977572E-4
16-17	5.609530667397651E-5	3.739687111598434E-5	1.869843555799217E-5	3.646194933808473E-4	6.918421156457103E-4
18-19	5.609530667397651E-5	5.609530667397651E-5	1.869843555799217E-5	4.113655822758277E-4	7.105405512037024E-4
20-21	6.544452445297259E-5	5.609530667397651E-5	1.869843555799217E-5	4.955085422867925E-4	7.946835112146672E-4
22-23	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	5.796515022977572E-4	8.414296001096476E-4
24-25	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	6.63794462308722E-4	8.601280356676398E-4
26-27	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	7.853342934356711E-4	9.629694312365967E-4
28-29	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	0.0012621444001644714	9.723186490155928E-4
30-31	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	0.002776717680361837	9.816678667945889E-4
32-33	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	0.005974150160778498	0.001093858480142542
34-35	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	0.010751600445845498	0.0011219061334795302
36-37	7.479374223196868E-5	5.609530667397651E-5	1.869843555799217E-5	0.016800544348855966	0.0011219061334795302
38-39	7.479374223196868E-5	6.544452445297259E-5	1.869843555799217E-5	0.026420889443442935	0.0011873506579325027
40-41	7.479374223196868E-5	7.479374223196868E-5	1.869843555799217E-5	0.03669567978255964	0.0011966998757114988
42-43	9.349217778996085E-5	9.349217778996085E-5	1.869843555799217E-5	0.04709200995280328	0.0012527951823854753
44-45	9.349217778996085E-5	9.349217778996085E-5	1.869843555799217E-5	0.058498055643178504	0.0013088904890594518
46-47	9.349217778996085E-5	9.349217778996085E-5	1.869843555799217E-5	0.07182069097824792	0.0013743350135124244
48-49	9.349217778996085E-5	9.349217778996085E-5	1.869843555799217E-5	0.0840588170509538	0.0014117318846284088
50-51	1.1219061334795302E-4	9.349217778996085E-5	1.869843555799217E-5	0.09665221339926153	0.001439779537965397
52-53	1.1219061334795302E-4	9.349217778996085E-5	1.869843555799217E-5	0.10903057773865234	0.0015239224979763618
54-55	1.1219061334795302E-4	9.349217778996085E-5	1.869843555799217E-5	0.1220259904514569	0.0015613193690923461
56-57	1.1219061334795302E-4	1.1219061334795302E-4	1.869843555799217E-5	0.13758308883570639	0.0016174146757663226
58-59	1.1219061334795302E-4	1.3088904890594518E-4	1.869843555799217E-5	0.15149472489085256	0.0016267638935453187
60-61	1.1219061334795302E-4	1.6828592002192952E-4	1.869843555799217E-5	0.16517263050152384	0.0016267638935453187
62-63	1.1219061334795302E-4	1.6828592002192952E-4	1.869843555799217E-5	0.17936474309003989	0.0016454623291033109
64-65	1.1219061334795302E-4	1.6828592002192952E-4	1.869843555799217E-5	0.193397918976313	0.0017389545068932717
66-67	1.1219061334795302E-4	2.2438122669590603E-4	1.869843555799217E-5	0.20860909630273963	0.00176700216023026
68-69	1.1219061334795302E-4	2.2438122669590603E-4	1.869843555799217E-5	0.22485803680263483	0.001776351378009256
70-71	1.1219061334795302E-4	2.2438122669590603E-4	1.869843555799217E-5	0.2419203592493027	0.001879192773578213
72-73	1.1219061334795302E-4	2.2438122669590603E-4	1.869843555799217E-5	0.25866480829148464	0.0020474786936001427
74-75	1.1219061334795302E-4	2.2438122669590603E-4	1.869843555799217E-5	0.2760356549248594	0.002140970871390103
76-77	1.1219061334795302E-4	2.2438122669590603E-4	1.869843555799217E-5	0.29387396244718395	0.002225113831401068
78-79	1.1219061334795302E-4	2.430796622538982E-4	1.869843555799217E-5	0.31405892363203647	0.0022625107025170525
80-81	1.1219061334795302E-4	2.6177809781189037E-4	1.869843555799217E-5	0.3345804566569329	0.002309256791412033
82-83	1.1219061334795302E-4	2.6177809781189037E-4	1.869843555799217E-5	0.35444754443729953	0.0023560028803070133
84-85	1.215398311269491E-4	2.7112731559088645E-4	1.869843555799217E-5	0.37459510875103613	0.0023747013158650055
86	1.3088904890594518E-4	2.8047653336988254E-4	1.869843555799217E-5	0.3901615563530646	0.0023747013158650055
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	20210	0.0	25.352427	1
TACATGG	20560	0.0	24.22299	2
GTACCTG	5535	0.0	23.37111	1
ACATGGG	20975	0.0	22.912916	3
TACCTGG	5790	0.0	21.780869	2
AGTACAT	14110	0.0	21.288021	2
GTATAGG	1090	0.0	21.117098	1
GAGTACA	15690	0.0	21.06649	1
CATGGGG	16160	0.0	20.148092	4
GTGTAGC	2840	0.0	17.344275	1
GTATACG	320	1.2210876E-8	17.263226	1
AGAGTAC	15935	0.0	16.276323	10-11
ATGGGGG	8145	0.0	15.539427	5
TACCGTA	630	0.0	15.277631	7
ACCTGGG	8055	0.0	15.14388	3
GTACCGT	980	0.0	14.970603	6
TAGACAG	2470	0.0	14.295876	5
TATATCG	385	1.243261E-7	14.293465	5
GTATCAA	29005	0.0	14.22086	1
GTCTTAG	2145	0.0	14.164698	1
>>END_MODULE