FastQCFastQC Report
Mon 15 Aug 2016
SRR2921444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5348041
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT171780.3212017260151895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68430.12795339452334042No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62450.1167717300596611No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC58570.10951673706316013No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT56660.10594533587158363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG204750.025.5885241
TACATGG212050.024.0357062
GTACCTG55200.023.2107771
ACATGGG211350.022.969753
AGTACAT144050.022.2250142
GAGTACA158450.022.0337371
TACCTGG58000.021.9483662
CATGGGG160250.019.6527394
GTATAGG12150.018.4245971
AGAGTAC163850.017.47200210-11
GTGTAGC28100.017.4585171
CGTATAC3059.289943E-817.13173
GTGTACG8100.016.4645351
TATAGGA13200.015.8333992
ATGGGGG74250.015.4732865
GTATAAG13400.015.2841091
TATACTG16500.015.2494995
CTACACT27050.015.1021524
GTATATA8850.015.0692351
ACCTGGG81650.014.9514933
Produced by FastQC (version 0.11.5)