##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921444_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5348041 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.887456173204356 33.0 31.0 34.0 30.0 34.0 2 31.99049315440925 34.0 31.0 34.0 30.0 34.0 3 32.122576098425576 34.0 31.0 34.0 30.0 34.0 4 35.679391201376355 37.0 35.0 37.0 35.0 37.0 5 35.48083101831119 37.0 35.0 37.0 33.0 37.0 6 35.52404721654153 37.0 35.0 37.0 33.0 37.0 7 35.44768822826901 37.0 35.0 37.0 33.0 37.0 8 35.39844346743041 37.0 35.0 37.0 33.0 37.0 9 37.0002546726923 39.0 37.0 39.0 33.0 39.0 10-11 36.90514115355511 39.0 37.0 39.0 33.0 39.0 12-13 36.78755491964254 39.0 37.0 39.0 33.0 39.0 14-15 38.058052378431654 40.0 38.0 41.0 33.0 41.0 16-17 37.959800513870405 40.0 38.0 41.0 33.0 41.0 18-19 37.840962980650296 40.0 38.0 41.0 33.0 41.0 20-21 37.712102618510215 40.0 38.0 41.0 32.0 41.0 22-23 37.681571064993705 40.0 38.0 41.0 32.5 41.0 24-25 37.74079677025662 40.0 38.0 41.0 32.5 41.0 26-27 37.6306783175372 40.0 38.0 41.0 32.0 41.0 28-29 37.490354131540876 40.0 37.5 41.0 32.0 41.0 30-31 37.35222355251203 40.0 37.0 41.0 32.0 41.0 32-33 37.19058660919016 40.0 37.0 41.0 31.0 41.0 34-35 37.0424304899682 40.0 37.0 41.0 31.0 41.0 36-37 36.94858416380877 40.0 37.0 41.0 31.0 41.0 38-39 36.91345896562872 40.0 37.0 41.0 31.0 41.0 40-41 36.74715592120555 39.5 36.0 41.0 30.0 41.0 42-43 36.49664690304357 39.0 36.0 41.0 30.0 41.0 44-45 36.28627949935313 39.0 35.5 40.5 30.0 41.0 46-47 36.0139442648252 39.0 35.0 40.0 29.0 41.0 48-49 35.671361719179046 39.0 35.0 40.0 28.0 41.0 50-51 35.36659086944172 38.0 34.5 40.0 27.5 41.0 52-53 35.035904735958454 38.0 34.0 40.0 27.0 41.0 54-55 34.68470314644185 38.0 34.0 40.0 26.0 41.0 56-57 34.26599188375706 37.0 33.0 40.0 25.5 41.0 58-59 33.846538573657156 37.0 33.0 39.5 25.0 41.0 60-61 33.36949613886655 36.0 32.5 39.0 23.0 40.0 62-63 32.90169989347501 35.5 32.0 39.0 22.5 40.0 64-65 32.35945031087084 35.0 31.0 38.0 21.5 40.0 66-67 31.79471127839147 35.0 31.0 38.0 19.5 40.0 68-69 31.25623849929348 34.5 30.0 37.0 18.0 39.0 70-71 30.675132726170197 34.0 29.5 36.0 14.5 39.0 72-73 30.101529887298916 34.0 29.0 36.0 11.5 38.5 74-75 29.559709714267335 34.0 29.0 35.0 7.5 37.5 76-77 28.354264299768833 31.5 27.0 34.0 7.5 36.0 78-79 29.29379580298655 33.5 29.0 35.0 7.0 36.0 80-81 29.313914384725173 34.0 29.0 35.0 6.0 36.0 82-83 28.960755816943063 34.0 29.0 35.0 2.0 36.0 84-85 28.442409285942276 33.0 28.5 35.0 2.0 35.0 86-87 27.906453690239097 33.0 27.0 35.0 2.0 35.0 88-89 27.337641484049954 33.0 26.0 35.0 2.0 35.0 90-91 26.82573207647436 32.0 25.0 34.0 2.0 35.0 92-93 26.341421746766713 32.0 25.0 34.0 2.0 35.0 94-95 25.790929613292043 32.0 23.5 34.0 2.0 35.0 96-97 24.943670121451948 31.5 19.0 34.0 2.0 35.0 98-99 23.849760968549045 31.0 5.0 34.0 2.0 35.0 100-101 20.251119148114235 25.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32353.0 3 17259.0 4 10678.0 5 8189.0 6 7319.0 7 7221.0 8 8775.0 9 10522.0 10 12833.0 11 14850.0 12 17290.0 13 20346.0 14 22660.0 15 25299.0 16 28529.0 17 33126.0 18 34259.0 19 33296.0 20 34042.0 21 36064.0 22 39317.0 23 43506.0 24 49597.0 25 57780.0 26 69190.0 27 83384.0 28 101523.0 29 123224.0 30 151352.0 31 188245.0 32 239281.0 33 317999.0 34 442335.0 35 646585.0 36 952821.0 37 1043828.0 38 375099.0 39 8065.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.059077198794995 17.07007626088902 13.307687359424285 24.5631591808917 2 16.612587674627026 18.7038955011751 37.6721494842691 27.01136733992877 3 21.734210817635656 21.337499102419876 26.71989441465808 30.208395665286385 4 14.38462354440719 14.104987335269167 33.570245524969415 37.94014359535423 5 16.201864841789867 35.357555159728456 32.00956467204462 16.431015326437066 6 36.82969857660081 31.959434517032726 15.604572794079264 15.6062941122872 7 32.77926339360478 29.410234738111953 18.071367316896733 19.739134551386538 8 28.922167591692066 32.74793937583434 17.92801048786386 20.401882544609734 9 26.952075342740073 16.602816781209683 18.3591970597736 38.085910816276645 10-11 27.6989344594888 24.278020129454976 25.057140012307656 22.96590539874857 12-13 27.844279913304387 21.181049478842795 25.776083617978195 25.198586989874627 14-15 24.493773732645174 22.931299166743845 23.574348021729957 29.00057907888102 16-17 25.037868386733773 26.022212628606617 24.14691849159432 24.793000493065286 18-19 25.0342219633362 25.58045462928981 25.211878753052293 24.173444654321692 20-21 25.764050351077582 24.35028529671251 25.114452545843207 24.771211806366697 22-23 25.38453717813925 24.05732003731147 25.137801861439318 25.42034092310996 24-25 25.339856564238023 24.320670319586192 25.17235522778124 25.167117888394543 26-27 25.219386282287253 24.327449648670555 25.52442258880816 24.92874148023403 28-29 24.956022164959784 24.97714723632353 25.2507164429083 24.81611415580839 30-31 25.249535186429366 24.574711710871096 24.968403148708738 25.2073499539908 32-33 25.13315420493901 24.556416000981347 25.041928872102588 25.26850092197705 34-35 25.408645246875754 24.468711156705737 25.23942943472494 24.883214161693573 36-37 25.28559030099115 24.4465428552354 25.314922131796695 24.95294471197676 38-39 25.19015066274195 24.643026559143795 25.253399409759403 24.91342336835485 40-41 25.266616357282658 24.672367150029505 25.12285121420483 24.938165278483012 42-43 25.150071949454844 24.638567469571658 24.915660055257703 25.29570052571579 44-45 25.25087039530169 24.338397649797102 25.00583006360426 25.404901891296944 46-47 25.417494823455083 24.678639006806613 25.13903401959297 24.76483215014533 48-49 25.388555112390122 24.66552229009946 24.92812118424276 25.017801413267666 50-51 24.91563574538619 24.911175837434204 25.164838945898417 25.008349471281182 52-53 25.300299275512046 24.966094262904328 24.728730729639494 25.004875731944132 54-55 25.14230995202918 24.541431166337624 24.984479697761746 25.33177918387145 56-57 25.306381640746505 24.631560878178572 24.967835292012353 25.09422218906257 58-59 25.66303704450956 24.58757601343534 24.73442570104201 25.01496124101309 60-61 25.41031083291283 24.658264806779755 25.142693335480033 24.78873102482738 62-63 25.054930135076987 24.819276162107343 25.091303992503057 25.034489710312613 64-65 25.09676902396405 24.8994956949834 24.91491366358329 25.088821617469257 66-67 25.309162793860263 24.71586091810601 24.647687553679862 25.327288734353864 68-69 25.02553191369937 24.795597350424895 24.885984581408987 25.292886154466743 70-71 25.272706718850618 24.62680922035346 24.945676520522113 25.154807540273804 72-73 25.419776786795634 24.500481499108687 24.99472768529626 25.085014028799417 74-75 24.991263392078757 25.204028792267785 24.720494024309843 25.08421379134361 76-77 25.20585737385792 24.60758599260374 24.7382140781672 25.44834255537114 78-79 25.141272802459458 24.639090090882384 25.009374526361878 25.210262580296277 80-81 25.27671207971328 24.72610297177785 24.936287248228247 25.060897700280627 82-83 25.325352847256493 24.58791351381075 25.031826649059024 25.054906989873732 84-85 25.179514566093687 24.87868173296394 24.83152435590158 25.11027934504079 86-87 25.159838294359304 24.90775121892319 24.89464703410177 25.037763452615735 88-89 24.99851994577457 24.787995153315766 24.708166196839507 25.505318704070156 90-91 25.171590374425467 24.582213315963507 24.795204624996934 25.450991684614095 92-93 25.405522502900098 24.90644018878644 24.83826148706831 24.849775821245153 94-95 25.36908661523044 24.70835009321016 24.774211500389054 25.14835179117034 96-97 24.95940327488976 24.657695370103383 25.222029118192758 25.160872236814104 98-99 25.13581413727436 24.955719743164693 24.707724124299073 25.20074199526188 100-101 25.658435988900095 24.092560506640766 24.935459552019026 25.31354395244011 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 71.0 1 69.0 2 126.0 3 222.0 4 203.5 5 169.5 6 370.5 7 961.0 8 1483.5 9 1433.5 10 1142.5 11 932.0 12 850.0 13 888.5 14 950.5 15 1011.0 16 1082.0 17 1182.0 18 1294.5 19 1349.0 20 1391.0 21 1467.0 22 1607.5 23 1718.0 24 1800.0 25 2072.5 26 2421.5 27 2792.5 28 3494.0 29 4568.0 30 6163.0 31 8581.0 32 11936.0 33 16464.0 34 22301.0 35 30188.5 36 40482.5 37 53027.0 38 66561.5 39 80605.5 40 96727.5 41 112011.0 42 127666.0 43 146101.5 44 165683.5 45 185769.5 46 206111.5 47 234434.0 48 273207.0 49 282385.5 50 263556.0 51 257351.0 52 259999.0 53 261910.5 54 260715.0 55 252809.5 56 237433.0 57 218703.5 58 201690.0 59 184039.5 60 162589.5 61 140495.0 62 114691.0 63 85695.5 64 62129.0 65 46150.0 66 34280.5 67 26003.0 68 19702.5 69 14534.0 70 11013.5 71 8247.0 72 5804.5 73 4233.0 74 3152.0 75 2240.0 76 1535.5 77 898.5 78 485.0 79 232.0 80 110.5 81 52.0 82 31.5 83 16.5 84 7.0 85 4.0 86 0.5 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2982400471499751 2 0.0 3 0.006151785298579423 4 0.05388889127813343 5 5.048577600657886E-4 6 0.06170483734137416 7 0.07161500818711002 8 0.10256091903558705 9 0.08935982353164458 10-11 0.09395963867891065 12-13 0.093108859861022 14-15 0.0625649653770418 16-17 0.03539613851127918 18-19 0.03850007881390588 20-21 0.02808505020810424 22-23 0.04172555894765952 24-25 0.033937660537755786 26-27 0.0212507720116581 28-29 0.02456039510542271 30-31 0.0037022902404824495 32-33 0.005497340054049698 34-35 0.008077724161052617 36-37 0.011368648819259239 38-39 0.016819242784413956 40-41 0.021054438438299183 42-43 0.003543353538239516 44-45 0.014173414152958063 46-47 0.0248315224210136 48-49 0.0028234637692568175 50-51 0.007423278916522891 52-53 0.0023560028803070133 54-55 0.003870576160504379 56-57 0.003300273875985618 58-59 0.0010377631734685654 60-61 0.005544086142944678 62-63 0.014846557833045781 64-65 0.007189548472047989 66-67 0.039481746680700466 68-69 0.06094755070127548 70-71 0.01263079321942371 72-73 0.03211456307085155 74-75 0.05080364941106473 76-77 0.2869087952018319 78-79 0.07051180048918847 80-81 0.033283215293226066 82-83 0.028038304119209257 84-85 0.019661404989228765 86-87 0.11660344413963918 88-89 0.5102803063776062 90-91 0.13047768332366935 92-93 0.04730704196172019 94-95 0.008376899129980492 96-97 0.005964800942999502 98-99 0.010611362179160557 100-101 0.007956184329925668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5348041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.1728724915498 #Duplication Level Percentage of deduplicated Percentage of total 1 67.25961183499227 29.03790645583255 2 16.188626206794883 13.978189900786342 3 6.310850221372581 8.17372595761762 4 3.158598694605988 5.454631147768 5 1.8142424167429259 3.916302826340175 6 1.097630807240199 2.8432724930266793 7 0.7574398476360317 2.2890597773406514 8 0.5331655236551667 1.841462973572391 9 0.392922783749616 1.526724471766227 >10 2.2323562730230977 17.69957996784355 >50 0.17171626739548004 5.082330057554149 >100 0.07962472276720015 5.818048713658719 >500 0.0019981436583146432 0.5670083485753992 >1k 9.99067729547039E-4 0.9880746423514577 >5k 1.7375090948642207E-4 0.4615378268848738 >10k+ 4.343772737160552E-5 0.3221444390812386 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17178 0.3212017260151895 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6843 0.12795339452334042 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6245 0.1167717300596611 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5857 0.10951673706316013 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5666 0.10594533587158363 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.869843555799217E-5 2 0.0 0.0 0.0 0.0 1.869843555799217E-5 3 0.0 0.0 0.0 0.0 1.869843555799217E-5 4 0.0 0.0 0.0 5.609530667397651E-5 3.739687111598434E-5 5 0.0 1.869843555799217E-5 0.0 5.609530667397651E-5 5.609530667397651E-5 6 1.869843555799217E-5 1.869843555799217E-5 0.0 5.609530667397651E-5 7.479374223196868E-5 7 1.869843555799217E-5 1.869843555799217E-5 0.0 7.479374223196868E-5 7.479374223196868E-5 8 1.869843555799217E-5 1.869843555799217E-5 0.0 7.479374223196868E-5 9.349217778996085E-5 9 1.869843555799217E-5 1.869843555799217E-5 0.0 1.1219061334795302E-4 9.349217778996085E-5 10-11 1.869843555799217E-5 1.869843555799217E-5 0.0 1.5893670224293344E-4 1.1219061334795302E-4 12-13 1.869843555799217E-5 1.869843555799217E-5 0.0 2.0568279113791386E-4 3.2722262226486296E-4 14-15 1.869843555799217E-5 1.869843555799217E-5 0.0 2.2438122669590603E-4 3.9266714671783555E-4 16-17 1.869843555799217E-5 1.869843555799217E-5 0.0 2.991749689278747E-4 4.955085422867925E-4 18-19 1.869843555799217E-5 5.609530667397651E-5 0.0 4.113655822758277E-4 5.048577600657886E-4 20-21 1.869843555799217E-5 5.609530667397651E-5 0.0 4.39413235612816E-4 5.048577600657886E-4 22-23 1.869843555799217E-5 5.609530667397651E-5 0.0 4.861593245077964E-4 5.796515022977572E-4 24-25 1.869843555799217E-5 5.609530667397651E-5 0.0 5.422546311817729E-4 6.544452445297259E-4 26-27 1.869843555799217E-5 5.609530667397651E-5 0.0 6.263975911927377E-4 6.544452445297259E-4 28-29 1.869843555799217E-5 5.609530667397651E-5 0.0 0.0010845092623635458 6.544452445297259E-4 30-31 1.869843555799217E-5 5.609530667397651E-5 0.0 0.002561685671444927 6.544452445297259E-4 32-33 1.869843555799217E-5 5.609530667397651E-5 0.0 0.005787165805198577 7.292389867616946E-4 34-35 1.869843555799217E-5 5.609530667397651E-5 0.0 0.010517870001370595 8.694772534466359E-4 36-37 1.869843555799217E-5 5.609530667397651E-5 0.0 0.01650136937992809 8.78826471225632E-4 38-39 1.869843555799217E-5 5.609530667397651E-5 0.0 0.025850587158924174 8.975249067836241E-4 40-41 1.869843555799217E-5 5.609530667397651E-5 0.0 0.03592904392468195 9.162233423416163E-4 42-43 1.869843555799217E-5 5.609530667397651E-5 0.0 0.04606359599711371 9.349217778996085E-4 44-45 1.869843555799217E-5 5.609530667397651E-5 0.0 0.057422895598593954 0.0010097155201315771 46-47 1.869843555799217E-5 5.609530667397651E-5 0.0 0.07063334032031543 0.0011219061334795302 48-49 1.869843555799217E-5 5.609530667397651E-5 0.0 0.08252554533519843 0.0012901920535014597 50-51 1.869843555799217E-5 6.544452445297259E-5 0.0 0.09493195732792625 0.001327588924617444 52-53 1.869843555799217E-5 7.479374223196868E-5 0.0 0.10688960686726223 0.0014678271913023853 54-55 1.869843555799217E-5 8.414296001096476E-5 0.0 0.11945495556223298 0.0015613193690923461 56-57 5.609530667397651E-5 9.349217778996085E-5 0.0 0.13450719618641666 0.0015987162402083305 58-59 5.609530667397651E-5 9.349217778996085E-5 0.0 0.14794202213483404 0.0016454623291033109 60-61 5.609530667397651E-5 9.349217778996085E-5 0.0 0.16120856216322949 0.0016735099824402991 62-63 5.609530667397651E-5 9.349217778996085E-5 0.0 0.17486776933834275 0.001776351378009256 64-65 6.544452445297259E-5 9.349217778996085E-5 0.0 0.1884428335534451 0.0018043990313462443 66-67 7.479374223196868E-5 9.349217778996085E-5 0.0 0.2032426452975959 0.0018324466846832326 68-69 7.479374223196868E-5 9.349217778996085E-5 0.0 0.21900542647298327 0.0018511451202412248 70-71 7.479374223196868E-5 1.0284139556895693E-4 0.0 0.2354413513284584 0.0019072404269152013 72-73 7.479374223196868E-5 1.1219061334795302E-4 0.0 0.25164354573945863 0.002140970871390103 74-75 7.479374223196868E-5 1.1219061334795302E-4 0.0 0.26851888383054656 0.0022625107025170525 76-77 7.479374223196868E-5 1.1219061334795302E-4 9.349217778996085E-6 0.2857401429794574 0.0022625107025170525 78-79 7.479374223196868E-5 1.3088904890594518E-4 1.869843555799217E-5 0.30528935735533813 0.002299907573633037 80-81 7.479374223196868E-5 1.3088904890594518E-4 1.869843555799217E-5 0.3249788099979039 0.002337304444749021 82-83 7.479374223196868E-5 1.3088904890594518E-4 1.869843555799217E-5 0.3443597384537628 0.002449495058096974 84-85 7.479374223196868E-5 1.3088904890594518E-4 1.869843555799217E-5 0.3638715559585276 0.0024588442758759705 86-87 8.414296001096476E-5 1.3088904890594518E-4 1.869843555799217E-5 0.38406586636115914 0.002552336453665931 88-89 9.349217778996085E-5 1.3088904890594518E-4 1.869843555799217E-5 0.40559711490618716 0.0026177809781189037 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 20475 0.0 25.588524 1 TACATGG 21205 0.0 24.035706 2 GTACCTG 5520 0.0 23.210777 1 ACATGGG 21135 0.0 22.96975 3 AGTACAT 14405 0.0 22.225014 2 GAGTACA 15845 0.0 22.033737 1 TACCTGG 5800 0.0 21.948366 2 CATGGGG 16025 0.0 19.652739 4 GTATAGG 1215 0.0 18.424597 1 AGAGTAC 16385 0.0 17.472002 10-11 GTGTAGC 2810 0.0 17.458517 1 CGTATAC 305 9.289943E-8 17.1317 3 GTGTACG 810 0.0 16.464535 1 TATAGGA 1320 0.0 15.833399 2 ATGGGGG 7425 0.0 15.473286 5 GTATAAG 1340 0.0 15.284109 1 TATACTG 1650 0.0 15.249499 5 CTACACT 2705 0.0 15.102152 4 GTATATA 885 0.0 15.069235 1 ACCTGGG 8165 0.0 14.951493 3 >>END_MODULE