FastQCFastQC Report
Mon 15 Aug 2016
SRR2921443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011500
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.14906192927112735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73350.032.382121
TCAACGC87000.026.9849414
TATCAAC88850.026.4230672
CAACGCA90100.025.9547085
GTACATG107900.025.8100951
ATCAACG91400.025.6858833
GGTATCA48700.025.1424941
AACGCAG93850.024.961096
TACATGG111450.024.3152162
ACATGGG113950.022.7757573
ACGCAGA110100.021.2283137
TCGCGTA2208.264033E-820.826849
GAGTACA81250.019.8856331
AGTACAT78150.019.8316332
CGCAGAG117950.019.7328688
CATGGGG91150.019.518474
GCAGAGT129150.017.8451279
AGAGTAC95050.017.59460810-11
GTATATA6000.016.8782391
GTACTTT46050.016.13094314-15
Produced by FastQC (version 0.11.5)