##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921443_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3011500
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.032687697160885	31.0	31.0	34.0	28.0	34.0
2	31.15725253196082	33.0	31.0	34.0	28.0	34.0
3	31.288387514527646	33.0	31.0	34.0	28.0	34.0
4	34.838426697658974	37.0	35.0	37.0	32.0	37.0
5	34.8363254192263	37.0	35.0	37.0	32.0	37.0
6	34.932442968620286	37.0	35.0	37.0	32.0	37.0
7	34.83926083347169	37.0	35.0	37.0	32.0	37.0
8	34.82859870496431	37.0	35.0	37.0	32.0	37.0
9	36.374140461564004	39.0	37.0	39.0	32.0	39.0
10-11	36.27029188112236	39.0	37.0	39.0	32.0	39.0
12-13	36.1328102274614	39.0	37.0	39.0	32.0	39.0
14-15	37.245933089822344	40.0	38.0	41.0	32.0	41.0
16-17	37.120527311970775	40.0	37.5	41.0	32.0	41.0
18-19	36.95796164701976	40.0	37.0	41.0	31.0	41.0
20-21	36.851262161713436	40.0	37.0	41.0	31.0	41.0
22-23	36.8990521334883	40.0	37.0	41.0	31.0	41.0
24-25	36.77984177320272	40.0	37.0	41.0	31.0	41.0
26-27	36.54001859538435	40.0	37.0	41.0	30.0	41.0
28-29	36.329509214677074	39.0	36.0	41.0	30.0	41.0
30-31	36.09473966461896	39.0	36.0	40.5	29.5	41.0
32-33	35.844018761414574	39.0	35.5	40.0	28.5	41.0
34-35	35.44311472688029	39.0	35.0	40.0	26.5	41.0
36-37	35.07781554042836	38.0	35.0	40.0	25.0	41.0
38-39	34.864622613315625	38.0	34.5	40.0	25.0	41.0
40-41	34.61275477336875	38.0	34.0	40.0	24.0	41.0
42-43	34.332087829985056	38.0	33.5	40.0	23.5	41.0
44-45	34.0233747302009	38.0	33.0	40.0	22.5	41.0
46-47	33.73958193591234	38.0	33.0	40.0	21.5	41.0
48-49	33.5489734351652	38.0	33.0	40.0	20.0	41.0
50-51	32.761239747634065	37.0	32.0	39.0	19.5	40.0
52-53	33.253933753943215	37.5	33.0	39.0	21.5	40.0
54-55	33.704350157728705	38.0	33.0	40.0	22.0	41.0
56-57	33.55934285239914	37.5	33.0	40.0	20.5	41.0
58-59	33.23371907687199	37.0	33.0	40.0	19.5	41.0
60-61	32.78427428191931	36.0	32.5	39.0	17.5	41.0
62-63	32.30931280092977	36.0	32.0	39.0	13.5	40.0
64-65	31.779716088328076	35.0	31.0	38.5	8.0	40.0
66-67	31.306584094305165	35.0	31.0	38.0	7.0	40.0
68-69	30.796144612319445	35.0	30.0	37.0	4.0	39.5
70-71	30.28669649676241	34.0	30.0	37.0	2.0	39.0
72-73	29.750260501411258	34.0	29.0	36.0	2.0	39.0
74-75	29.173038352980242	34.0	29.0	35.5	2.0	38.0
76-77	28.608613979744312	34.0	28.0	35.0	2.0	37.0
78-79	28.07109496928441	33.0	27.0	35.0	2.0	36.5
80-81	27.512637556035198	33.0	26.0	35.0	2.0	36.0
82-83	26.907229287730367	32.5	25.5	35.0	2.0	35.5
84-85	26.307051303337207	32.0	24.5	34.0	2.0	35.0
86-87	25.710190104599036	32.0	23.5	34.0	2.0	35.0
88-89	25.080758592063756	31.0	20.0	34.0	2.0	35.0
90-91	24.357561514195584	31.0	17.5	34.0	2.0	35.0
92-93	23.545934584094304	30.5	4.5	34.0	2.0	35.0
94-95	22.63110161049311	30.0	2.0	34.0	2.0	35.0
96-97	21.72573169516852	29.0	2.0	34.0	2.0	35.0
98	21.090451270131165	29.0	2.0	34.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59055.0
3	10636.0
4	8436.0
5	7382.0
6	7183.0
7	7479.0
8	8111.0
9	9607.0
10	10909.0
11	12546.0
12	14578.0
13	17957.0
14	14652.0
15	14206.0
16	15995.0
17	18593.0
18	21301.0
19	20645.0
20	21276.0
21	23517.0
22	25765.0
23	29223.0
24	32589.0
25	38074.0
26	43373.0
27	50411.0
28	59039.0
29	70226.0
30	85113.0
31	105872.0
32	134494.0
33	178468.0
34	246901.0
35	354223.0
36	487337.0
37	514640.0
38	222023.0
39	9665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58787175407912	17.153954418248713	13.264203556989864	24.993970270682304
2	16.442288047332955	18.730435042597332	38.09601799236919	26.731258917700522
3	20.359196290161073	21.82537072142918	27.37331815696116	30.44211483144858
4	14.10484028507755	14.394841441228337	34.06252782403119	37.43779044966292
5	16.06795645534493	35.50941425235539	32.23761766984794	16.185011622451732
6	35.894630340627224	32.57355225536991	16.073159345360423	15.458658058642442
7	31.581636398728225	30.134097115850643	18.977862863965193	19.306403621455935
8	28.188854304081495	32.910743298806096	18.87619746020865	20.02420493690376
9	27.070134660913464	16.08787613414351	18.733970223761812	38.10801898118121
10-11	26.980243478561206	24.5542918268929	25.690551680827017	22.774913013718876
12-13	27.01050551992685	21.692340313822292	26.40970382002151	24.887450346229354
14-15	24.213887912409433	23.278963953544558	23.840054491704944	28.66709364234106
16-17	24.597700996735114	26.390283252372615	24.51167019966916	24.500345551223113
18-19	24.642424224302147	25.59674592380809	26.028888464731143	23.73194138715862
20-21	25.205140927521803	24.707953961228025	25.786374940221577	24.300530171028594
22-23	24.930751835552083	24.44015225206315	25.787456372451505	24.84163953993326
24-25	24.615780288661547	24.73907417769738	25.8431866544209	24.80195887922017
26-27	24.586226309499594	24.862197292666163	25.953186627371377	24.598389770462862
28-29	24.600000631444576	25.051450270333053	25.829506308380584	24.519042789841787
30-31	24.78959210171343	24.871854845461662	25.686309167046527	24.65224388577838
32-33	24.61225282243673	24.92352345333357	25.586289742767427	24.877933981462274
34-35	24.81646757353506	24.956643094622144	25.735458956490753	24.491430375352046
36-37	24.785526093350857	24.912917479915727	25.650872094771444	24.650684331961976
38-39	24.81300586590318	24.930597748440192	25.59188721197043	24.6645091736862
40-41	24.864903670770158	24.95894978224811	25.572628624711268	24.603517922270463
42-43	24.90143910589162	24.839581921545197	25.464162557876357	24.794816414686824
44-45	24.809262793206774	24.88891707393596	25.441457107596694	24.860363025260572
46-47	24.954565941418224	24.927616715595224	25.507000040548988	24.610817302437564
48-49	24.85849283270619	25.019054496527477	25.29085637781696	24.831596292949367
50-51	24.754470659049762	25.178937334761127	25.256751775272296	24.809840230916812
52-53	24.86116935559968	25.175013206259305	25.242201042104544	24.72161639603647
54-55	24.813654868982393	24.92822348192414	25.366496010300825	24.891625638792643
56-57	24.80932923275752	25.026602118423153	25.34676211831634	24.817306530502982
58-59	24.903529758818973	25.0184581146377	25.3287713494688	24.74924077707453
60-61	24.96410534705173	24.871123010358218	25.39914653829364	24.76562510429641
62-63	24.744838737080464	25.079078921038573	25.35452207258621	24.82156026929475
64-65	24.78111529666512	24.973529853134295	25.340581443504785	24.9047734066958
66-67	24.757292576652535	25.123424138235034	25.284316318434275	24.834966966678156
68-69	24.816456107095405	25.056680770728306	25.25403082265682	24.87283229951947
70-71	24.731036077204326	24.89724266211439	25.39713015073189	24.974591109949394
72-73	24.868974385274097	25.01627344389102	25.213289270367657	24.90146290046723
74-75	24.693847060428208	25.139340815311765	25.25344471038487	24.913367413875154
76-77	24.86449571861338	24.9344721457631	25.248265390102738	24.952766745520783
78-79	24.79493666833203	24.856053779055223	25.353712151582442	24.995297401030303
80-81	24.800791348395567	25.067531253475785	25.213474467358125	24.918202930770523
82-83	24.89780424014206	25.059089155532533	25.16600087297337	24.877105731352035
84-85	24.94239073329873	25.080459566973058	25.083560738699106	24.89358896102911
86-87	24.85584908730822	25.017382144061205	25.235771902251823	24.89099686637875
88-89	24.762566485136734	24.882415268194592	25.20672080487147	25.148297441797208
90-91	24.85877034569124	24.891887069636834	25.184601557119677	25.064741027552245
92-93	24.857722218211123	25.042390283408373	25.217754079496807	24.882133418883697
94-95	24.883442089587582	24.87842257016071	25.27458105356544	24.963554286686268
96-97	24.89263487051317	24.898604695217102	25.231113925933485	24.977646508336242
98	24.973671274777182	24.933019322849866	25.111401021667923	24.98190838070503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	474.0
1	362.0
2	417.5
3	616.5
4	538.5
5	492.5
6	876.5
7	1948.0
8	2790.5
9	2550.0
10	1888.5
11	1491.0
12	1538.5
13	1709.0
14	1913.5
15	2130.0
16	2346.5
17	2462.5
18	2465.0
19	2397.0
20	2246.5
21	2133.5
22	2074.0
23	2078.0
24	2181.5
25	2462.5
26	2916.0
27	3687.0
28	4945.0
29	6876.5
30	9448.0
31	12692.5
32	16615.0
33	20948.0
34	26362.0
35	32537.5
36	39013.0
37	46327.0
38	54067.5
39	61571.5
40	68905.5
41	77261.0
42	86688.0
43	96566.0
44	105632.5
45	114248.5
46	124598.5
47	134885.5
48	139618.0
49	138077.5
50	136376.0
51	135827.5
52	136244.5
53	138462.5
54	137657.0
55	133133.0
56	125454.0
57	116922.0
58	108953.5
59	99154.0
60	87126.0
61	72579.5
62	58107.5
63	44871.5
64	33532.0
65	25567.0
66	19952.0
67	15596.5
68	12268.0
69	9702.0
70	7574.0
71	5891.0
72	4760.5
73	3699.5
74	2935.0
75	2335.5
76	1645.5
77	1043.0
78	645.0
79	398.5
80	245.0
81	153.5
82	76.0
83	44.0
84	24.0
85	14.5
86	14.0
87	11.5
88	7.0
89	6.0
90	7.5
91	4.5
92	5.0
93	6.5
94	4.5
95	2.0
96	1.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46000332060435
2	0.04433006807238917
3	0.05173501577287066
4	0.05020753777187448
5	0.060036526647849905
6	0.06325751286734185
7	0.0404781670263988
8	0.01806408766395484
9	0.010194255354474513
10-11	0.013049975095467375
12-13	0.009081852897227296
14-15	0.013265814378216834
16-17	0.012253030051469369
18-19	0.05357795118711606
20-21	0.08205213348829486
22-23	0.09750954673750623
24-25	0.06720903204383197
26-27	0.08261663622779346
28-29	0.08389506890254025
30-31	0.05419226299186451
32-33	0.031097459737672258
34-35	0.38816204549227956
36-37	0.6097293707454756
38-39	0.5585422546903537
40-41	0.5542918811223643
42-43	0.5276440312136809
44-45	0.5122862360949693
46-47	0.5022248049144944
48-49	0.4925120371907687
50-51	0.5070396812219824
52-53	0.5862360949692844
54-55	0.5574630582766064
56-57	0.5146272621617134
58-59	0.4931263489955172
60-61	0.5057944545907355
62-63	0.5180308816204549
64-65	0.5360949692844097
66-67	0.5627760252365931
68-69	0.5165034036194587
70-71	0.46573136310808566
72-73	0.4488626930101278
74-75	0.42892246388842764
76-77	0.4432176656151419
78-79	0.43692512037190767
80-81	0.4341192096961647
82-83	0.4549061929271127
84-85	0.4195085505562013
86-87	0.42281255188444294
88-89	0.4501245226631247
90-91	0.4321766561514196
92-93	0.4275776191266811
94-95	0.4384858044164038
96-97	0.43456749128341354
98	0.42749460401793127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3011500.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.27103372798086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73887875566852	40.01885176012377
2	14.1920166557129	15.404308291805219
3	5.067507450695501	8.250566033204697
4	2.2850480478802457	4.960476787062626
5	1.2369713629529249	3.356585727968232
6	0.7763722768904252	2.528071561475374
7	0.5263492179861256	1.999586131341496
8	0.3754975890787837	1.6302913857336132
9	0.27017000553846166	1.3196164934579968
>10	1.41527488262804	13.60827875933447
>50	0.08259095798845993	3.0534507975807488
>100	0.03185040177168231	3.010667797630136
>500	0.001165646206633172	0.4244801329256471
>1k	3.067490014165359E-4	0.43476834035605355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4489	0.14906192927112735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.961813049975096E-5
2	0.0	0.0	0.0	0.0	9.961813049975096E-5
3	0.0	0.0	0.0	0.0	9.961813049975096E-5
4	0.0	0.0	0.0	3.3206043499916987E-5	9.961813049975096E-5
5	6.641208699983397E-5	0.0	0.0	3.3206043499916987E-5	1.3282417399966795E-4
6	6.641208699983397E-5	0.0	0.0	3.3206043499916987E-5	1.6603021749958492E-4
7	6.641208699983397E-5	0.0	0.0	3.3206043499916987E-5	1.6603021749958492E-4
8	6.641208699983397E-5	0.0	0.0	3.3206043499916987E-5	1.6603021749958492E-4
9	6.641208699983397E-5	0.0	0.0	9.961813049975096E-5	1.6603021749958492E-4
10-11	1.3282417399966795E-4	0.0	0.0	1.9923626099950192E-4	1.6603021749958492E-4
12-13	1.3282417399966795E-4	0.0	0.0	2.656483479993359E-4	5.811057612485472E-4
14-15	1.3282417399966795E-4	1.6603021749958493E-5	0.0	2.656483479993359E-4	7.139299352482152E-4
16-17	1.4942719574962643E-4	3.3206043499916987E-5	0.0	3.9847252199900384E-4	8.965631744977585E-4
18-19	1.6603021749958492E-4	3.3206043499916987E-5	0.0	5.312966959986718E-4	9.297692179976756E-4
20-21	1.6603021749958492E-4	3.3206043499916987E-5	0.0	5.312966959986718E-4	9.961813049975095E-4
22-23	1.6603021749958492E-4	3.3206043499916987E-5	0.0	6.143118047484642E-4	0.0010625933919973436
24-25	1.6603021749958492E-4	3.3206043499916987E-5	0.0	6.973269134982567E-4	0.0010957994354972606
26-27	1.6603021749958492E-4	6.641208699983397E-5	0.0	8.301510874979246E-4	0.0011622115224970944
28-29	1.6603021749958492E-4	6.641208699983397E-5	0.0	0.0012286236094969285	0.001278432674746804
30-31	1.6603021749958492E-4	6.641208699983397E-5	0.0	0.0037190768719907023	0.0012950356964967623
32-33	1.6603021749958492E-4	6.641208699983397E-5	0.0	0.009297692179976755	0.0013614477834965964
34-35	1.6603021749958492E-4	9.961813049975096E-5	0.0	0.016984891250207537	0.0013946538269965134
36-37	1.6603021749958492E-4	1.1622115224970946E-4	0.0	0.027610825170180972	0.001411256848746472
38-39	1.6603021749958492E-4	1.3282417399966795E-4	0.0	0.043898389506890255	0.0015440810227461397
40-41	1.9923626099950192E-4	1.3282417399966795E-4	0.0	0.061630416735845926	0.0015606840444960983
42-43	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.07972771044330068	0.0015606840444960983
44-45	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.09898721567325253	0.0016270961314959324
46-47	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.11932591731695169	0.0017101112402457246
48-49	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.14120869998339697	0.001793126348995517
50-51	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.16282583430184294	0.0018263323924954341
52-53	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.18426033538103936	0.0019591565664951022
54-55	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.20692346006973267	0.0019591565664951022
56-57	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.22988543914992526	0.002058774696994853
58-59	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.25397642370911505	0.002158392827494604
60-61	1.9923626099950192E-4	1.6603021749958492E-4	0.0	0.27854889589905363	0.002241407936244396
62-63	1.9923626099950192E-4	1.9923626099950192E-4	0.0	0.30459903702473845	0.002457247218993857
64-65	1.9923626099950192E-4	1.9923626099950192E-4	0.0	0.33046654491117383	0.002523659305993691
66-67	1.9923626099950192E-4	2.324423044994189E-4	0.0	0.35942221484310144	0.0025402623277436497
68-69	1.9923626099950192E-4	2.324423044994189E-4	0.0	0.3902540262327744	0.002556865349493608
70-71	1.9923626099950192E-4	2.324423044994189E-4	0.0	0.4182135148597045	0.002573468371243566
72-73	1.9923626099950192E-4	2.324423044994189E-4	0.0	0.4461065913996347	0.002673086501743317
74-75	1.9923626099950192E-4	2.324423044994189E-4	0.0	0.47587580939731033	0.0028391167192429023
76-77	2.158392827494604E-4	2.656483479993359E-4	0.0	0.5071392993524821	0.0029221318279926945
78-79	2.324423044994189E-4	2.656483479993359E-4	0.0	0.5393491615474015	0.0029885439149925285
80-81	2.490453262493774E-4	2.656483479993359E-4	0.0	0.5746803918313133	0.003005146936742487
82-83	2.656483479993359E-4	2.656483479993359E-4	0.0	0.6089988377884775	0.0031545741324921135
84-85	2.656483479993359E-4	2.656483479993359E-4	0.0	0.6436659472023909	0.0031877801759920307
86	2.656483479993359E-4	2.656483479993359E-4	0.0	0.67199070230782	0.0031877801759920307
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7335	0.0	32.38212	1
TCAACGC	8700	0.0	26.984941	4
TATCAAC	8885	0.0	26.423067	2
CAACGCA	9010	0.0	25.954708	5
GTACATG	10790	0.0	25.810095	1
ATCAACG	9140	0.0	25.685883	3
GGTATCA	4870	0.0	25.142494	1
AACGCAG	9385	0.0	24.96109	6
TACATGG	11145	0.0	24.315216	2
ACATGGG	11395	0.0	22.775757	3
ACGCAGA	11010	0.0	21.228313	7
TCGCGTA	220	8.264033E-8	20.82684	9
GAGTACA	8125	0.0	19.885633	1
AGTACAT	7815	0.0	19.831633	2
CGCAGAG	11795	0.0	19.732868	8
CATGGGG	9115	0.0	19.51847	4
GCAGAGT	12915	0.0	17.845127	9
AGAGTAC	9505	0.0	17.594608	10-11
GTATATA	600	0.0	16.878239	1
GTACTTT	4605	0.0	16.130943	14-15
>>END_MODULE