FastQCFastQC Report
Mon 15 Aug 2016
SRR2921443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011500
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47240.15686534949360784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75600.032.5758061
TCAACGC92600.025.7490184
TATCAAC96450.025.0162472
CAACGCA95450.024.9179955
ATCAACG96950.024.6912693
GGTATCA49750.023.9371991
GTACATG108950.023.5223881
AACGCAG100800.023.5020486
TACATGG111400.022.597032
GAGTACA83800.022.2827321
AGTACAT83950.021.2729552
ACATGGG116600.020.7745843
ACGCAGA117500.020.1627647
AGAGTAC98800.018.81302510-11
CGCAGAG126800.018.6090628
GTATAAC4101.8189894E-1218.5892771
CATGGGG96150.017.8824584
GCAGAGT140050.016.882389
CGTATAC2002.763792E-416.623743
TACTTTT54600.015.64566414-15
Produced by FastQC (version 0.11.5)