##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3011500
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.124792628258344	31.0	31.0	34.0	28.0	34.0
2	31.2538472521999	31.0	31.0	34.0	28.0	34.0
3	31.380672090320438	33.0	31.0	34.0	28.0	34.0
4	35.03271592229786	37.0	35.0	37.0	32.0	37.0
5	34.70391167192429	37.0	35.0	37.0	32.0	37.0
6	34.73043699153246	37.0	35.0	37.0	32.0	37.0
7	34.62757961148929	37.0	35.0	37.0	32.0	37.0
8	34.555869500249045	37.0	35.0	37.0	32.0	37.0
9	36.053233936576454	39.0	37.0	39.0	32.0	39.0
10-11	35.90823360451603	39.0	36.0	39.0	30.5	39.0
12-13	35.743181471027725	39.0	35.0	39.0	30.0	39.0
14-15	36.820387016436996	40.0	37.0	41.0	31.0	41.0
16-17	36.66895168520671	40.0	36.5	41.0	30.5	41.0
18-19	36.526251867839946	39.0	36.0	41.0	30.0	41.0
20-21	36.36760966295866	39.0	36.0	40.5	30.0	41.0
22-23	36.302725884110906	39.0	36.0	40.0	29.5	41.0
24-25	36.329292213182796	39.0	36.0	41.0	30.0	41.0
26-27	36.167267806740824	39.0	36.0	40.5	29.0	41.0
28-29	35.97380325419226	39.0	36.0	40.0	29.0	41.0
30-31	35.77536344014611	39.0	35.0	40.0	28.0	41.0
32-33	35.547818196911834	38.5	35.0	40.0	27.0	41.0
34-35	35.3435435829321	38.0	35.0	40.0	27.0	41.0
36-37	35.18125983729038	38.0	35.0	40.0	26.5	41.0
38-39	35.09211024406442	38.0	35.0	40.0	26.0	41.0
40-41	34.86035148597045	38.0	34.0	40.0	25.5	41.0
42-43	34.52709247883115	38.0	34.0	40.0	24.0	41.0
44-45	34.24004283579612	38.0	33.0	40.0	24.0	41.0
46-47	33.87179744313465	38.0	33.0	40.0	22.5	41.0
48-49	33.421907189108424	37.5	33.0	40.0	20.5	41.0
50-51	33.03864087663955	37.0	32.0	40.0	18.5	40.5
52-53	32.62689340860037	36.5	31.5	39.0	17.5	40.0
54-55	32.199613149593226	36.0	31.0	39.0	13.5	40.0
56-57	31.70620903204383	35.5	30.5	39.0	10.0	40.0
58-59	31.19923443466711	35.0	29.5	38.5	8.0	40.0
60-61	30.662690353644365	35.0	29.0	38.0	6.5	40.0
62-63	30.129903868504066	34.0	28.0	38.0	2.0	40.0
64-65	29.491868670097958	34.0	28.0	37.0	2.0	39.0
66-67	28.860454590735515	33.5	26.5	36.5	2.0	39.0
68-69	28.25896828822846	33.0	26.0	36.0	2.0	39.0
70-71	27.60425834301843	32.5	25.5	35.0	2.0	38.0
72-73	26.960580275610162	32.0	24.0	35.0	2.0	37.5
74-75	26.35721999003819	31.0	22.5	35.0	2.0	36.5
76-77	25.457714594056117	30.5	22.5	33.5	2.0	35.0
78-79	26.417326913498258	31.5	24.5	34.0	2.0	36.0
80-81	26.403389672920472	32.0	24.5	35.0	2.0	36.0
82-83	25.95476174663789	32.0	23.5	34.0	2.0	35.0
84-85	25.309393491615474	31.0	20.5	34.0	2.0	35.0
86-87	24.621120039847252	31.0	18.5	34.0	2.0	35.0
88-89	23.935114892910512	30.5	14.0	34.0	2.0	35.0
90-91	23.234443964801592	30.0	7.0	34.0	2.0	35.0
92-93	22.57972787647352	29.5	2.0	34.0	2.0	35.0
94-95	21.811280591067572	29.0	2.0	34.0	2.0	35.0
96-97	20.726189440478166	29.0	2.0	33.5	2.0	34.5
98-99	19.39546023576291	27.0	2.0	33.0	2.0	34.0
100-101	16.151606010293875	13.5	2.0	30.0	2.0	33.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43781.0
3	19903.0
4	11614.0
5	8453.0
6	7308.0
7	7602.0
8	8722.0
9	10294.0
10	12092.0
11	13988.0
12	16160.0
13	18820.0
14	20856.0
15	23545.0
16	26465.0
17	29715.0
18	30382.0
19	29167.0
20	30109.0
21	31646.0
22	33728.0
23	37209.0
24	41956.0
25	48067.0
26	56803.0
27	68766.0
28	82817.0
29	99332.0
30	120605.0
31	146775.0
32	183296.0
33	232246.0
34	298956.0
35	380010.0
36	420452.0
37	296382.0
38	62745.0
39	733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65977923859597	18.092862873934987	13.798403758026422	24.448954129442615
2	16.797111074215508	19.602424041175496	37.25761248547236	26.34285239913664
3	20.555553895163513	22.572830606471477	27.736517035456345	29.135098462908672
4	14.73874286929336	15.194476837870074	34.03698498606252	36.02979530677405
5	16.09621701460144	35.730291112288825	32.23503760936469	15.93845426374505
6	35.84759593532456	33.05472639460737	15.830325317569383	15.267352352498692
7	31.63142291111051	30.274916578665273	18.984708691569345	19.10895181865488
8	28.309620771858167	33.13320250540796	18.696294405377774	19.860882317356108
9	27.095174632505675	16.156689011562403	18.644018225990315	38.104118129941604
10-11	27.098503862279088	24.76001489640072	25.640454662611646	22.50102657870855
12-13	27.1812734107293	21.828305254306578	26.41672510693739	24.573696228026733
14-15	24.235940619620486	23.363090639838575	23.95861887023907	28.442349870301868
16-17	24.56870830143824	26.459610555716196	24.64643572432076	24.325245418524805
18-19	24.66975249229222	25.697096911190304	26.13552243258151	23.497628163935968
20-21	25.275320400987205	24.71782907706945	25.94187203424298	24.064978487700365
22-23	24.979458201446985	24.461856367359612	25.989432039824266	24.569253391369138
24-25	24.6483667399255	24.822088658964773	25.996389042099665	24.533155559010066
26-27	24.579021642344507	24.888081748233006	26.17746237590517	24.35543423351732
28-29	24.61066645055477	25.143450912818118	25.982833054225708	24.263049582401404
30-31	24.803455529893853	24.919331674085683	25.81107489693349	24.46613789908697
32-33	24.586338227837004	24.939055309983573	25.825970324809212	24.64863613737021
34-35	24.889120905742434	24.87442613603438	25.952839585750294	24.283613372472896
36-37	24.792145127376337	25.020345044651165	25.78697256270848	24.400537265264013
38-39	24.835820082589787	24.963749897875342	25.766416497233834	24.434013522301036
40-41	24.889493284904894	24.92820260234222	25.769096513570844	24.41320759918204
42-43	24.906903827519542	24.882629419785204	25.65160679311497	24.55885995958029
44-45	24.880787258247253	24.832084263984513	25.662874652308275	24.62425382545996
46-47	24.877470945173844	25.018894376326156	25.6541539479407	24.4494807305593
48-49	24.866628777633714	25.09386335474678	25.45570195908881	24.583805908530696
50-51	24.766349165761042	25.223879233979595	25.394272032384922	24.615499567874433
52-53	24.918593600258216	25.215262894687378	25.32130871870345	24.544834786350954
54-55	24.935046261274753	24.95744467475644	25.414993288777815	24.692515775190994
56-57	24.902287712791956	25.09376062631501	25.348094834548274	24.65585682634476
58-59	25.041948049834918	25.037265940175924	25.323339519695242	24.59744649029392
60-61	25.068167259591483	24.979734955466714	25.422560631585313	24.529537153356493
62-63	24.857362856372838	25.05863819243091	25.44066445935213	24.643334491844122
64-65	24.93257038184325	24.98532189548127	25.348538565557327	24.733569157118147
66-67	24.9114489094339	25.083468724681595	25.282046393031642	24.72303597285286
68-69	24.952396520784554	25.035659001268872	25.259331394972158	24.752613082974417
70-71	24.90587843610336	24.95571018545348	25.39456500170617	24.743846376736993
72-73	25.092137178977225	24.93871223023375	25.224436718021455	24.74471387276757
74-75	24.872760856854303	25.170294477223198	25.112588369744326	24.844356296178173
76-77	24.89241699460965	24.92970033618284	25.370323159482616	24.807559509724893
78-79	24.88310952100407	24.88319259291011	25.43626872897658	24.797429157109242
80-81	24.83091253090775	25.094400665388505	25.293133663636013	24.781553140067736
82-83	24.96153306412706	25.045134814881244	25.184670355174582	24.808661765817114
84-85	24.94498018157329	25.088871142613794	25.25865085244393	24.70749782336899
86-87	24.87979600517334	25.000236859256237	25.262942862400063	24.85702427317036
88-89	24.835032512912385	24.878051548084535	25.2982630855962	24.988652853406883
90-91	24.89855221838578	24.94902259363867	25.189554826686795	24.962870361288754
92-93	24.977766600633664	25.070038945407664	25.240879821375355	24.711314632583317
94-95	24.91723561473789	24.885537909558757	25.285470296643364	24.91175617905999
96-97	24.84933134949	24.89215300964542	25.347716363536136	24.910799277328447
98-99	24.956890146046305	24.854920916145627	25.21271817488196	24.975470762926108
100-101	25.554636601424612	24.123199038546318	25.397991912723967	24.9241724473051
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	264.0
1	226.5
2	311.5
3	488.0
4	449.0
5	395.0
6	734.5
7	1695.5
8	2544.5
9	2375.5
10	1640.0
11	1145.0
12	1054.0
13	1116.5
14	1147.0
15	1183.0
16	1252.0
17	1302.5
18	1364.5
19	1408.5
20	1473.0
21	1513.0
22	1551.5
23	1754.5
24	1967.5
25	2243.0
26	2697.0
27	3332.5
28	4379.5
29	5950.0
30	8076.5
31	10818.5
32	14169.0
33	17990.0
34	22386.5
35	27794.5
36	34195.0
37	40953.0
38	48126.0
39	54994.5
40	62353.5
41	70315.0
42	78545.0
43	88224.5
44	98218.5
45	108097.0
46	115918.0
47	124345.0
48	134800.5
49	138672.0
50	136125.5
51	133701.0
52	134081.0
53	135034.5
54	134427.5
55	131002.5
56	123285.5
57	114016.0
58	105902.0
59	97546.0
60	85599.5
61	72520.0
62	59599.5
63	46024.5
64	34813.0
65	26757.0
66	20652.5
67	16156.0
68	12793.5
69	10137.0
70	8030.5
71	6357.5
72	4889.5
73	3803.5
74	3026.5
75	2435.0
76	1818.0
77	1193.5
78	794.5
79	487.0
80	288.5
81	170.5
82	98.0
83	52.5
84	26.0
85	15.5
86	7.5
87	3.0
88	2.5
89	2.5
90	1.0
91	1.0
92	1.0
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3012784326747468
2	0.0
3	0.005412585090486468
4	0.054125850904864686
5	5.312966959986718E-4
6	0.058575460733853564
7	0.0690021583928275
8	0.09855553710775361
9	0.08666777353478333
10-11	0.09030383529802424
12-13	0.09065249875477337
14-15	0.06040179312634899
16-17	0.032525319608168686
18-19	0.035480657479661296
20-21	0.025917316951685206
22-23	0.0390004980906525
24-25	0.03126348995517184
26-27	0.01982400796945044
28-29	0.023709115058940727
30-31	0.003154574132492114
32-33	0.005412585090486468
34-35	0.007454756765731363
36-37	0.010775361115723062
38-39	0.01573966461896065
40-41	0.01982400796945044
42-43	0.0032541922629918643
44-45	0.013000166030217501
46-47	0.022696330732193258
48-49	0.0024738502407438156
50-51	0.007388344678731528
52-53	0.002191598870994521
54-55	0.00418396148098954
56-57	0.003586252697991034
58-59	0.0012286236094969285
60-61	0.0052133488294869666
62-63	0.0149593225967126
64-65	0.007039681221982401
66-67	0.03622779345840943
68-69	0.05781172173335548
70-71	0.012203220986219492
72-73	0.030134484476174665
74-75	0.047152581769882115
76-77	0.2928274946040179
78-79	0.06837124356632908
80-81	0.030815208367922963
82-83	0.026481819691183794
84-85	0.017914660468205213
86-87	0.11238585422546903
88-89	0.5031379711107422
90-91	0.12601693508218498
92-93	0.04612319442138469
94-95	0.008002656483479994
96-97	0.005761248547235597
98-99	0.009878797941225302
100-101	0.007521168852731197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3011500.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.17462591299363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67215182492579	39.911612653206255
2	14.24961932856631	15.439355930552864
3	5.060604633556864	8.22469088749516
4	2.311915752745517	5.0098868418938185
5	1.2635305037097142	3.4225646184065095
6	0.7618242521809778	2.4762926324010355
7	0.5006939227447044	1.8987434173122584
8	0.35464079048098157	1.5370025726236425
9	0.2765167123059386	1.3482170503078597
>10	1.4325604616710428	13.79487145147632
>50	0.08112105934216923	2.9746684793611746
>100	0.03328708365529766	3.061937342188263
>500	0.0011655923274195214	0.3911034615764083
>1k	3.680817873128722E-4	0.5090526611984906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4724	0.15686534949360784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.961813049975096E-5
2	0.0	0.0	0.0	3.3206043499916987E-5	9.961813049975096E-5
3	0.0	0.0	0.0	3.3206043499916987E-5	9.961813049975096E-5
4	0.0	0.0	0.0	6.641208699983397E-5	9.961813049975096E-5
5	0.0	0.0	0.0	6.641208699983397E-5	9.961813049975096E-5
6	0.0	0.0	0.0	6.641208699983397E-5	1.3282417399966795E-4
7	0.0	0.0	0.0	1.3282417399966795E-4	1.3282417399966795E-4
8	0.0	0.0	0.0	1.3282417399966795E-4	1.3282417399966795E-4
9	0.0	0.0	0.0	1.3282417399966795E-4	1.3282417399966795E-4
10-11	0.0	0.0	0.0	1.3282417399966795E-4	1.3282417399966795E-4
12-13	0.0	0.0	0.0	1.6603021749958492E-4	5.312966959986718E-4
14-15	0.0	3.3206043499916987E-5	0.0	1.6603021749958492E-4	6.309148264984227E-4
16-17	1.6603021749958493E-5	3.3206043499916987E-5	0.0	1.992362609995019E-4	6.973269134982567E-4
18-19	3.3206043499916987E-5	3.3206043499916987E-5	0.0	2.324423044994189E-4	8.633571309978416E-4
20-21	3.3206043499916987E-5	3.3206043499916987E-5	0.0	2.324423044994189E-4	9.629752614975925E-4
22-23	3.3206043499916987E-5	3.3206043499916987E-5	0.0	2.822513697492944E-4	0.001045990370247385
24-25	3.3206043499916987E-5	3.3206043499916987E-5	0.0	3.3206043499916984E-4	0.0010957994354972606
26-27	3.3206043499916987E-5	3.3206043499916987E-5	0.0	3.984725219990038E-4	0.001112402457247219
28-29	3.3206043499916987E-5	3.3206043499916987E-5	0.0	7.803420222480492E-4	0.0011290054789971774
30-31	3.3206043499916987E-5	3.3206043499916987E-5	0.0	0.0031545741324921135	0.0011290054789971774
32-33	3.3206043499916987E-5	3.3206043499916987E-5	0.0	0.008284907853229289	0.0011954175659970115
34-35	3.3206043499916987E-5	3.3206043499916987E-5	0.0	0.01552382533621119	0.0013282417399966794
36-37	3.3206043499916987E-5	6.641208699983397E-5	0.0	0.025684874647185787	0.0013282417399966794
38-39	3.3206043499916987E-5	1.1622115224970946E-4	0.0	0.041158890918147104	0.0014444628922463888
40-41	3.3206043499916987E-5	1.3282417399966795E-4	0.0	0.057961148929105095	0.0014942719574962643
42-43	3.3206043499916987E-5	1.6603021749958492E-4	0.0	0.07458077370081355	0.0015606840444960983
44-45	3.3206043499916987E-5	1.6603021749958492E-4	0.0	0.09239581603851901	0.0016104931097459738
46-47	3.3206043499916987E-5	1.6603021749958492E-4	0.0	0.11150589407272124	0.0016603021749958492
48-49	3.3206043499916987E-5	1.6603021749958492E-4	0.0	0.13201062593392	0.0017765233272455587
50-51	3.3206043499916987E-5	1.6603021749958492E-4	0.0	0.1521999003818695	0.0018263323924954341
52-53	3.3206043499916987E-5	1.6603021749958492E-4	0.0	0.17220654159056947	0.0020421716752448945
54-55	4.980906524987548E-5	1.8263323924954343E-4	0.0	0.1936410426697659	0.002125186783994687
56-57	6.641208699983397E-5	1.9923626099950192E-4	0.0	0.21500913166196248	0.0021417898057446453
58-59	6.641208699983397E-5	1.9923626099950192E-4	0.0	0.23729038685040676	0.002158392827494604
60-61	6.641208699983397E-5	2.324423044994189E-4	0.0	0.26046820521334885	0.0021749958492445626
62-63	6.641208699983397E-5	2.324423044994189E-4	0.0	0.28475842603353807	0.002291217001494272
64-65	6.641208699983397E-5	2.324423044994189E-4	0.0	0.30833471691847913	0.002324423044994189
66-67	6.641208699983397E-5	2.324423044994189E-4	0.0	0.33504897891416235	0.0023410260667441475
68-69	6.641208699983397E-5	2.324423044994189E-4	0.0	0.36378880956334053	0.002357629088494106
70-71	6.641208699983397E-5	2.324423044994189E-4	0.0	0.3898721567325253	0.002457247218993857
72-73	6.641208699983397E-5	2.324423044994189E-4	0.0	0.4159721069234601	0.0026564834799933587
74-75	6.641208699983397E-5	2.324423044994189E-4	0.0	0.4440976257678898	0.0028391167192429023
76-77	6.641208699983397E-5	2.324423044994189E-4	0.0	0.4734849742653163	0.002905528806242736
78-79	6.641208699983397E-5	2.324423044994189E-4	0.0	0.5041175493939898	0.0029719408932425704
80-81	6.641208699983397E-5	2.324423044994189E-4	0.0	0.5368919143284078	0.0029885439149925285
82-83	6.641208699983397E-5	2.324423044994189E-4	0.0	0.5692512037190769	0.003021749958492446
84-85	6.641208699983397E-5	2.324423044994189E-4	0.0	0.6017101112402458	0.003021749958492446
86-87	6.641208699983397E-5	2.324423044994189E-4	0.0	0.6374896231114062	0.003171177154242072
88-89	6.641208699983397E-5	2.324423044994189E-4	0.0	0.6725219990038187	0.003337207371741657
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7560	0.0	32.575806	1
TCAACGC	9260	0.0	25.749018	4
TATCAAC	9645	0.0	25.016247	2
CAACGCA	9545	0.0	24.917995	5
ATCAACG	9695	0.0	24.691269	3
GGTATCA	4975	0.0	23.937199	1
GTACATG	10895	0.0	23.522388	1
AACGCAG	10080	0.0	23.502048	6
TACATGG	11140	0.0	22.59703	2
GAGTACA	8380	0.0	22.282732	1
AGTACAT	8395	0.0	21.272955	2
ACATGGG	11660	0.0	20.774584	3
ACGCAGA	11750	0.0	20.162764	7
AGAGTAC	9880	0.0	18.813025	10-11
CGCAGAG	12680	0.0	18.609062	8
GTATAAC	410	1.8189894E-12	18.589277	1
CATGGGG	9615	0.0	17.882458	4
GCAGAGT	14005	0.0	16.88238	9
CGTATAC	200	2.763792E-4	16.62374	3
TACTTTT	5460	0.0	15.645664	14-15
>>END_MODULE