FastQCFastQC Report
Mon 15 Aug 2016
SRR2921442_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921442_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221348
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT271660.4366577789893766No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT118910.19113221121853335No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT108360.1741744715132476No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC96200.1546288682131268No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT86670.1393106445741341No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT68610.11028156598859283No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC65590.1054273125374115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG213800.026.0934431
GTACCTG63150.025.5112151
TACATGG219200.024.7678642
ACATGGG231300.022.976573
TACCTGG68150.022.405562
GAGTACA164500.020.510421
CATGGGG178100.020.1850384
AGTACAT155450.020.1173632
ACCTGGG87750.016.9307163
AGAGTAC173250.016.51812610-11
GTGTAGC29300.016.1810261
GTATAGG13200.016.040591
ATGGGGG89550.014.7982265
GTATATG20000.014.2691521
GTCCTAG23750.014.1480221
GTATAAT13700.014.111241
GTACACG15100.014.0222381
TAGACTG23050.013.7263665
GTCTTAG22950.013.6383771
GTACTAG7850.013.4863571
Produced by FastQC (version 0.11.5)