##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921442_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6221348
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.58031506998162	31.0	30.0	34.0	26.0	34.0
2	29.650553545630302	31.0	30.0	34.0	25.0	34.0
3	29.78310922327444	31.0	30.0	34.0	25.0	34.0
4	33.25707772656344	37.0	35.0	37.0	27.0	37.0
5	33.20845916351247	37.0	35.0	37.0	27.0	37.0
6	33.31075853657439	37.0	35.0	37.0	28.0	37.0
7	33.16901079958877	37.0	35.0	37.0	27.0	37.0
8	33.13587521546777	37.0	35.0	37.0	27.0	37.0
9	34.48189508125892	38.0	35.0	39.0	27.0	39.0
10-11	34.32229735420684	38.0	35.0	39.0	27.0	39.0
12-13	34.12910441595616	38.0	35.0	39.0	25.0	39.0
14-15	34.997819363263396	39.0	35.0	40.0	25.0	41.0
16-17	34.83103187605001	39.0	34.5	40.0	24.5	41.0
18-19	34.61532581041922	38.5	34.0	40.0	23.0	41.0
20-21	34.47199819074579	39.0	34.0	40.0	21.5	41.0
22-23	34.49189203047314	39.0	34.0	40.0	20.5	41.0
24-25	34.32033588219144	39.0	34.0	40.0	18.5	41.0
26-27	33.999112973587074	38.0	34.0	40.0	17.5	41.0
28-29	33.721261935516225	38.0	33.0	40.0	16.0	41.0
30-31	33.43447312383104	38.0	33.0	40.0	14.0	41.0
32-33	33.12111418618602	38.0	32.5	40.0	9.5	41.0
34-35	32.6556977683936	38.0	31.5	40.0	8.5	41.0
36-37	32.23457392192174	37.5	31.0	40.0	8.0	41.0
38-39	31.954641582499484	37.0	30.5	40.0	4.0	41.0
40-41	31.657127603213965	37.0	30.0	40.0	2.0	41.0
42-43	31.3155606309115	37.0	30.0	39.5	2.0	40.5
44-45	30.954552855747664	36.0	29.5	39.0	2.0	40.0
46-47	30.626415448870567	36.0	29.0	39.0	2.0	40.0
48-49	30.362958718914292	36.0	28.0	39.0	2.0	40.0
50-51	29.671666735247733	35.0	27.5	38.5	2.0	39.5
52-53	30.27631294696905	35.5	28.5	38.5	2.0	39.5
54-55	30.681432625212416	36.0	29.0	39.0	2.0	40.0
56-57	30.470300086090667	36.0	29.0	39.0	2.0	40.0
58-59	30.094617758080723	35.0	28.0	39.0	2.0	40.0
60-61	29.564255608270106	35.0	28.0	38.5	2.0	40.0
62-63	29.04519141189337	34.5	26.5	38.0	2.0	40.0
64-65	28.448719393289043	34.0	26.0	37.5	2.0	40.0
66-67	27.902464787374058	34.0	25.5	37.0	2.0	39.0
68-69	27.33044791900405	33.5	24.0	36.0	2.0	39.0
70-71	26.74980558875665	33.0	22.5	36.0	2.0	39.0
72-73	26.154429554495263	32.5	20.5	35.0	2.0	38.0
74-75	25.51277592894659	32.0	18.5	35.0	2.0	37.0
76-77	24.89037134717428	31.5	15.0	35.0	2.0	36.5
78-79	24.26857033234598	31.0	7.5	34.0	2.0	36.0
80-81	23.609890493185723	30.5	4.0	34.0	2.0	35.5
82-83	22.914228475886574	30.0	2.0	34.0	2.0	35.0
84-85	22.228103861092485	29.5	2.0	34.0	2.0	35.0
86-87	21.56389282515622	29.0	2.0	34.0	2.0	35.0
88-89	20.828973801176208	28.5	2.0	34.0	2.0	35.0
90-91	20.00982166565831	27.0	2.0	33.0	2.0	34.5
92-93	19.11835136050901	25.5	2.0	33.0	2.0	34.0
94-95	18.15741106268288	24.5	2.0	32.0	2.0	34.0
96-97	17.232042637704883	21.5	2.0	32.0	2.0	34.0
98	16.602169979882174	18.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	288562.0
3	47972.0
4	35748.0
5	30090.0
6	28630.0
7	29657.0
8	34149.0
9	38770.0
10	43007.0
11	46848.0
12	50113.0
13	52560.0
14	46436.0
15	47888.0
16	52233.0
17	58634.0
18	65055.0
19	67187.0
20	71830.0
21	78049.0
22	85202.0
23	93830.0
24	103356.0
25	115158.0
26	128382.0
27	144347.0
28	163556.0
29	188848.0
30	218917.0
31	258903.0
32	313149.0
33	389740.0
34	496896.0
35	639019.0
36	764981.0
37	667921.0
38	228138.0
39	7587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.636470231307065	16.757147846349863	12.818564500193673	24.7878174221494
2	16.12345638909745	18.727652788393303	38.37670015696416	26.772190665545086
3	21.393395589812272	21.76180110219313	26.96757941432079	29.87722389367381
4	13.968608069812163	14.001028579258016	34.49138556830612	37.538977782623704
5	15.755079458770057	35.66129864949845	32.426254766641634	16.157367125089863
6	35.80372364721852	32.52785658682827	16.139771871924037	15.528647894029168
7	31.867502804752405	29.719971782832005	18.983136752764835	19.42938865965075
8	28.241499834170945	32.88562971875296	18.547839069775367	20.32503137730073
9	26.934565863830716	16.723927122004635	18.84225853754675	37.49924847661789
10-11	27.364829400544803	24.382648521476746	25.540284523994206	22.712237553984245
12-13	27.67484212964667	21.410913988286655	26.105454164911123	24.80878971715555
14-15	24.35787378068601	23.080984087146607	24.045174138566864	28.515967993600523
16-17	24.80035926089482	26.131769076973754	24.433494535610926	24.634377126520505
18-19	24.736130773574143	25.948262317153837	25.428363833090202	23.88724307618182
20-21	25.502184431699494	24.653659114120813	25.22366717815565	24.620489276024045
22-23	25.055182656826418	24.38982932090118	25.342754305389537	25.212233716882864
24-25	25.25875819549061	24.57235796104767	25.334981103506333	24.83390273995539
26-27	25.094233041611663	24.51268791009159	25.751360862419638	24.641718185877114
28-29	24.79324457931606	25.325443729869395	25.350338031640135	24.530973659174407
30-31	25.097774079216524	24.76664044991073	25.10193134211966	25.033654128753085
32-33	24.961598622783956	24.91236639709263	25.20941583198723	24.91661914813618
34-35	25.401439055997855	24.694302246935763	25.31782272595851	24.586435971107875
36-37	25.292599582326286	24.54790280322575	25.495251855698843	24.664245758749125
38-39	25.04928923226127	24.88137575279257	25.411386004565017	24.65794901038114
40-41	25.156978764688564	24.96975404970105	25.186754047357805	24.68651313825258
42-43	24.95488971133212	24.91986294647311	24.934532827696348	25.190714514498424
44-45	25.123727209389067	24.53407540312608	25.13719961107729	25.204997776407563
46-47	25.494886480604357	24.873378500580802	25.110892998462848	24.520842020351992
48-49	25.372238068592797	24.850205565843293	25.02990155079444	24.747654814769472
50-51	24.555193541248364	25.09122599007862	25.55023931173847	24.80334115693455
52-53	25.03045819651427	25.286522859307908	24.89531295781763	24.78770598636019
54-55	24.80969416770379	24.783804526420766	25.26450825923191	25.141993046643535
56-57	25.108445568421978	24.70233554256304	25.25740400229875	24.931814886716232
58-59	25.596754085455682	24.68032574141122	24.95302862600999	24.769891547123112
60-61	25.189141350161727	24.82645839439498	25.498317545541116	24.486082709902174
62-63	24.80622891340779	25.009440476774163	25.36142991539475	24.822900694423296
64-65	24.803214524303513	25.271778493448643	25.155279779095167	24.769727203152673
66-67	25.11810165906366	24.811473711532315	24.893429717431985	25.176994911972038
68-69	24.766389034549093	24.917102920322844	25.180640191941915	25.135867853186145
70-71	25.15629871846273	24.686292458279034	25.119088005717323	25.038320817540914
72-73	25.172518285872776	24.472782762249267	25.44995048534692	24.904748466531032
74-75	24.687451828285543	25.421437431853416	25.09100872679788	24.80010201306316
76-77	24.96923851315552	24.723219264262077	24.985438540470366	25.32210368211204
78-79	24.987160555806888	24.742664284552593	25.270608992816936	24.999566166823588
80-81	25.20289782198455	24.78802620562933	25.107553703000768	24.901522269385353
82-83	25.178600043026915	24.524784673699777	25.383353265908355	24.913262017364953
84-85	24.948033454205284	24.909329897464627	25.197258212925405	24.945378435404685
86-87	24.995024700566063	24.965092201619633	25.177646032099844	24.862237065714467
88-89	24.75954026952284	24.828324349265554	24.940480904123582	25.471654477088023
90-91	25.00843018971599	24.461884323753505	25.06847864494527	25.46120684158523
92-93	25.35924995442112	24.79431395088231	25.09532422422889	24.751111870467685
94-95	25.341768345024455	24.534403158593214	25.11525861038461	25.008569885997716
96-97	24.79225288908919	24.537298596681985	25.5974303280435	25.07301818618532
98	25.077380641087476	24.98456906698381	24.710621582516428	25.227428709412287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	607.0
1	456.0
2	429.5
3	580.0
4	537.5
5	517.0
6	794.5
7	1499.5
8	2230.0
9	2262.5
10	1925.5
11	1870.0
12	2055.5
13	2430.5
14	2935.0
15	3452.0
16	3922.0
17	4039.0
18	3979.5
19	3883.5
20	3653.0
21	3437.0
22	3140.0
23	3031.5
24	3217.0
25	3624.0
26	4400.5
27	5714.5
28	7894.0
29	10995.0
30	15146.5
31	20847.5
32	28305.0
33	36713.5
34	46909.5
35	59358.0
36	72878.5
37	87626.5
38	103562.0
39	119713.0
40	137014.0
41	155707.5
42	174902.0
43	193136.5
44	211844.5
45	234798.0
46	274362.5
47	315268.5
48	323690.5
49	306744.5
50	295347.0
51	293566.0
52	292261.5
53	292079.5
54	288380.5
55	279067.0
56	263738.5
57	246256.0
58	226606.5
59	202908.5
60	178148.5
61	150892.0
62	122484.5
63	95053.0
64	71108.0
65	53893.0
66	41125.0
67	31211.0
68	23017.0
69	16780.0
70	12422.0
71	9463.5
72	7166.5
73	5052.5
74	3649.0
75	2625.5
76	1743.0
77	1093.0
78	659.0
79	403.0
80	245.0
81	139.5
82	84.5
83	55.0
84	43.5
85	33.0
86	28.5
87	22.5
88	17.5
89	13.0
90	11.0
91	11.5
92	9.5
93	8.5
94	8.5
95	9.5
96	8.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.44972568645894745
2	0.04389723899064961
3	0.051435798158212656
4	0.04921762936263974
5	0.05879754676960684
6	0.060887126069784234
7	0.039203722408712706
8	0.017472097686867864
9	0.010447896500886946
10-11	0.012585696861837659
12-13	0.009306664729251603
14-15	0.013534044390379706
16-17	0.012842875852628723
18-19	0.05161260871438152
20-21	0.07806989739201214
22-23	0.09408732641221805
24-25	0.06521094785245898
26-27	0.08000677666640735
28-29	0.08142929795922041
30-31	0.0532762353110612
32-33	0.029647915532132262
34-35	0.3760599792842323
36-37	0.5885541204253484
38-39	0.5406786439208995
40-41	0.5357279483481715
42-43	0.5110709126060783
44-45	0.4969180312691076
46-47	0.48795695080873147
48-49	0.4786663597664043
50-51	0.49310856746801496
52-53	0.5691772908379342
54-55	0.5391918278803887
56-57	0.4987745421088806
58-59	0.4791726809045242
60-61	0.4909145092028287
62-63	0.5022625321714844
64-65	0.5214062932984941
66-67	0.5446086603739254
68-69	0.5005667582009558
70-71	0.45390484505930223
72-73	0.43755790545714535
74-75	0.4189285023117177
76-77	0.43260720988441737
78-79	0.4270858984258717
80-81	0.424658771700281
82-83	0.4428381116118243
84-85	0.41044159561561255
86-87	0.4137527751220475
88-89	0.4392536794276739
90-91	0.42355772414595677
92-93	0.4186311391036155
94-95	0.4290308145437291
96-97	0.4229951451036013
98	0.41791586003547787
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6221348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.00450602791727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12221422055694	35.83317987545941
2	13.290357823841822	13.025748401832683
3	5.242684242944072	7.707454547574589
4	2.5631252656914922	5.024187501315431
5	1.4639901350870885	3.5871056699843322
6	0.9404073972152288	2.7650519979319004
7	0.613355071268225	2.104001360105215
8	0.4592948436567263	1.8006013547653892
9	0.32426862564903325	1.4301561438254249
>10	1.8079153438817583	16.082474423572847
>50	0.1227291762651633	4.117309326149365
>100	0.04774356712710308	3.8854306456791505
>500	9.901566786598987E-4	0.3314374820923941
>1k	5.940836593950157E-4	0.7263799414773221
>5k	2.310325341138269E-4	0.7725236917147741
>10k+	9.90139431916401E-5	0.8069576365196637
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	27166	0.4366577789893766	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	11891	0.19113221121853335	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	10836	0.1741744715132476	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	9620	0.1546288682131268	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	8667	0.1393106445741341	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	6861	0.11028156598859283	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	6559	0.1054273125374115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6073686924441455E-5
2	0.0	0.0	0.0	0.0	1.6073686924441455E-5
3	0.0	0.0	0.0	0.0	1.6073686924441455E-5
4	0.0	0.0	0.0	0.0	1.6073686924441455E-5
5	0.0	0.0	0.0	0.0	1.6073686924441455E-5
6	0.0	0.0	0.0	0.0	1.6073686924441455E-5
7	0.0	0.0	0.0	0.0	1.6073686924441455E-5
8	0.0	0.0	0.0	1.6073686924441455E-5	1.6073686924441455E-5
9	0.0	0.0	0.0	4.8221060773324366E-5	1.6073686924441455E-5
10-11	0.0	0.0	0.0	7.233159115998655E-5	1.6073686924441455E-5
12-13	0.0	0.0	0.0	8.036843462220728E-5	3.214737384888291E-5
14-15	0.0	1.6073686924441455E-5	0.0	8.840527808442801E-5	6.429474769776582E-5
16-17	0.0	1.6073686924441455E-5	0.0	1.6073686924441457E-4	6.429474769776582E-5
18-19	0.0	1.6073686924441455E-5	0.0	1.6073686924441457E-4	8.036843462220728E-5
20-21	0.0	1.6073686924441455E-5	0.0	2.1699477347995964E-4	8.036843462220728E-5
22-23	8.036843462220728E-6	1.6073686924441455E-5	0.0	3.375474254132706E-4	9.644212154664875E-5
24-25	1.6073686924441455E-5	3.214737384888291E-5	0.0	4.259527034976986E-4	1.125158084710902E-4
26-27	1.6073686924441455E-5	4.8221060773324366E-5	0.0	5.786527292798924E-4	1.2858949539553164E-4
28-29	1.6073686924441455E-5	4.8221060773324366E-5	0.0	0.0011573054585597847	1.3662633885775237E-4
30-31	1.6073686924441455E-5	4.8221060773324366E-5	0.0	0.0025557162209861916	1.446631823199731E-4
32-33	1.6073686924441455E-5	4.8221060773324366E-5	0.0	0.0064696589870876856	1.5270002578219384E-4
34-35	1.6073686924441455E-5	4.8221060773324366E-5	0.0	0.011122991351713487	1.9288424309329746E-4
36-37	1.6073686924441455E-5	4.8221060773324366E-5	0.0	0.017496208217254523	2.250316169421804E-4
38-39	1.6073686924441455E-5	5.62579042355451E-5	0.0	0.02758244676234154	2.250316169421804E-4
40-41	3.214737384888291E-5	6.429474769776582E-5	0.0	0.039187648721788274	2.250316169421804E-4
42-43	3.214737384888291E-5	8.036843462220728E-5	0.0	0.0505999664381417	2.330684604044011E-4
44-45	3.214737384888291E-5	1.125158084710902E-4	0.0	0.06272756322263277	2.4914214732884253E-4
46-47	3.214737384888291E-5	1.125158084710902E-4	0.0	0.07633393920417247	2.571789907910633E-4
48-49	3.214737384888291E-5	1.125158084710902E-4	0.0	0.08883123078792571	2.571789907910633E-4
50-51	3.214737384888291E-5	1.125158084710902E-4	0.0	0.10207594881366547	2.571789907910633E-4
52-53	3.214737384888291E-5	1.125158084710902E-4	0.0	0.1148464930751342	2.8128952117772543E-4
54-55	3.214737384888291E-5	1.125158084710902E-4	0.0	0.12847697958706056	3.214737384888291E-4
56-57	3.214737384888291E-5	1.125158084710902E-4	0.0	0.14498465605846192	3.4558426887549133E-4
58-59	3.214737384888291E-5	1.125158084710902E-4	0.0	0.15986085330703248	3.5362111233771203E-4
60-61	3.214737384888291E-5	1.125158084710902E-4	0.0	0.1752915927544963	3.6165795579993273E-4
62-63	3.214737384888291E-5	1.125158084710902E-4	0.0	0.19073840588888452	4.018421731110364E-4
64-65	4.0184217311103635E-5	1.125158084710902E-4	0.0	0.20549405048552177	4.0987901657325713E-4
66-67	5.62579042355451E-5	1.2858949539553164E-4	0.0	0.22216246382616756	4.179158600354778E-4
68-69	6.429474769776582E-5	1.2858949539553164E-4	0.0	0.2399319247211376	4.179158600354778E-4
70-71	6.429474769776582E-5	1.3662633885775237E-4	0.0	0.25708254866951663	4.179158600354778E-4
72-73	6.429474769776582E-5	1.446631823199731E-4	0.0	0.27420906208750906	5.143579815821266E-4
74-75	6.429474769776582E-5	1.446631823199731E-4	0.0	0.292123186164799	5.384685119687888E-4
76-77	6.429474769776582E-5	1.6073686924441457E-4	0.0	0.309964978650929	5.62579042355451E-4
78-79	6.429474769776582E-5	1.6073686924441457E-4	1.6073686924441455E-5	0.3307000347834585	5.786527292798924E-4
80-81	6.429474769776582E-5	1.6073686924441457E-4	2.4110530386662183E-5	0.3518128225587124	6.18836946590996E-4
82-83	6.429474769776582E-5	1.6073686924441457E-4	3.214737384888291E-5	0.37139057323268204	6.590211639020997E-4
84-85	6.429474769776582E-5	1.6073686924441457E-4	3.214737384888291E-5	0.3913219450189894	6.911685377509826E-4
86	6.429474769776582E-5	1.6073686924441457E-4	3.214737384888291E-5	0.40664820550144437	6.911685377509826E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	21380	0.0	26.093443	1
GTACCTG	6315	0.0	25.511215	1
TACATGG	21920	0.0	24.767864	2
ACATGGG	23130	0.0	22.97657	3
TACCTGG	6815	0.0	22.40556	2
GAGTACA	16450	0.0	20.51042	1
CATGGGG	17810	0.0	20.185038	4
AGTACAT	15545	0.0	20.117363	2
ACCTGGG	8775	0.0	16.930716	3
AGAGTAC	17325	0.0	16.518126	10-11
GTGTAGC	2930	0.0	16.181026	1
GTATAGG	1320	0.0	16.04059	1
ATGGGGG	8955	0.0	14.798226	5
GTATATG	2000	0.0	14.269152	1
GTCCTAG	2375	0.0	14.148022	1
GTATAAT	1370	0.0	14.11124	1
GTACACG	1510	0.0	14.022238	1
TAGACTG	2305	0.0	13.726366	5
GTCTTAG	2295	0.0	13.638377	1
GTACTAG	785	0.0	13.486357	1
>>END_MODULE