FastQCFastQC Report
Mon 15 Aug 2016
SRR2921442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221348
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT285180.4583894037112214No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT117580.18899441085758262No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT109490.1759907981357095No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC95820.15401806810999802No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT90400.14530612979695076No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC69110.11108525033481491No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT67880.10910818684310859No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG62220.10001048004387474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG213050.026.5110761
TACATGG221100.024.9642122
GTACCTG65300.024.9423351
ACATGGG225350.023.6086653
TACCTGG69250.022.4985522
GAGTACA161100.021.8164961
CATGGGG170850.021.3808924
AGTACAT154950.020.9990442
GTATAGC12450.017.2134271
AGAGTAC170150.016.65913210-11
ACCTGGG89750.016.4073433
GTATAGG15500.016.2842711
ATGGGGG79750.016.2523355
GTGTAGC30350.015.2207911
TAGACTG24900.014.8723325
CGTATAC3858.0806785E-814.8057873
ATGGGGA79250.014.7373585
ACACCGT13650.014.6089126
TATACTG21450.014.6083265
TACACTG45100.014.3168065
Produced by FastQC (version 0.11.5)