##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6221348
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.839486394266967	33.0	31.0	34.0	30.0	34.0
2	31.94285370308814	34.0	31.0	34.0	30.0	34.0
3	32.07578646942753	34.0	31.0	34.0	30.0	34.0
4	35.63864053256626	37.0	35.0	37.0	35.0	37.0
5	35.4335558788867	37.0	35.0	37.0	33.0	37.0
6	35.47443367578859	37.0	35.0	37.0	33.0	37.0
7	35.39769725146383	37.0	35.0	37.0	33.0	37.0
8	35.34891457606937	37.0	35.0	37.0	33.0	37.0
9	36.94173834995245	39.0	37.0	39.0	33.0	39.0
10-11	36.841952981893954	39.0	37.0	39.0	33.0	39.0
12-13	36.72580427907264	39.0	37.0	39.0	33.0	39.0
14-15	37.978527081269206	40.0	38.0	41.0	33.0	41.0
16-17	37.87982564228845	40.0	38.0	41.0	33.0	41.0
18-19	37.76028040868314	40.0	38.0	41.0	32.5	41.0
20-21	37.62921628881715	40.0	38.0	41.0	32.0	41.0
22-23	37.59757451279047	40.0	38.0	41.0	32.0	41.0
24-25	37.654734954546825	40.0	38.0	41.0	32.0	41.0
26-27	37.53937940780679	40.0	38.0	41.0	32.0	41.0
28-29	37.398139760064865	40.0	37.0	41.0	32.0	41.0
30-31	37.25898294067459	40.0	37.0	41.0	31.5	41.0
32-33	37.09540094847611	40.0	37.0	41.0	31.0	41.0
34-35	36.93634675314739	40.0	37.0	41.0	31.0	41.0
36-37	36.83850581899614	40.0	36.5	41.0	30.5	41.0
38-39	36.79571469077119	40.0	36.0	41.0	30.0	41.0
40-41	36.632206798269436	39.5	36.0	41.0	30.0	41.0
42-43	36.375654842005304	39.0	36.0	41.0	30.0	41.0
44-45	36.159605603158674	39.0	35.0	40.0	29.5	41.0
46-47	35.887379792932336	39.0	35.0	40.0	29.0	41.0
48-49	35.53684940948489	38.0	35.0	40.0	28.0	41.0
50-51	35.22742032755602	38.0	34.5	40.0	27.0	41.0
52-53	34.898127945904974	38.0	34.0	40.0	26.0	41.0
54-55	34.54573695282759	38.0	34.0	40.0	26.0	41.0
56-57	34.11927045392735	37.0	33.0	40.0	24.5	41.0
58-59	33.6967002167376	37.0	33.0	39.5	23.5	41.0
60-61	33.20737210006577	36.0	32.0	39.0	22.5	40.0
62-63	32.744032402624	35.5	31.5	39.0	21.5	40.0
64-65	32.194496353523384	35.0	31.0	38.0	20.0	40.0
66-67	31.61969721031519	35.0	30.5	38.0	18.0	40.0
68-69	31.074069638927128	34.5	30.0	37.0	15.5	39.0
70-71	30.47733296706759	34.0	29.0	36.5	12.0	39.0
72-73	29.89565332143452	34.0	29.0	36.0	8.0	38.5
74-75	29.3463475278991	34.0	28.5	35.0	7.0	37.5
76-77	28.156801227000965	31.5	27.0	34.0	6.0	36.0
78-79	29.08520741807081	33.5	29.0	35.0	4.0	36.0
80-81	29.093497100628355	34.0	29.0	35.0	2.0	36.0
82-83	28.722110465448967	34.0	29.0	35.0	2.0	36.0
84-85	28.189753008511982	33.0	27.5	35.0	2.0	35.0
86-87	27.64888204292703	33.0	26.5	35.0	2.0	35.0
88-89	27.060137047469453	32.5	25.5	34.5	2.0	35.0
90-91	26.525842148679033	32.0	25.0	34.0	2.0	35.0
92-93	26.028585605563293	32.0	24.0	34.0	2.0	35.0
94-95	25.45121081476233	32.0	21.5	34.0	2.0	35.0
96-97	24.58709969286399	31.0	18.0	34.0	2.0	35.0
98-99	23.480672918473616	31.0	2.0	34.0	2.0	35.0
100-101	19.919134566978087	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40436.0
3	21283.0
4	12800.0
5	10092.0
6	8910.0
7	9075.0
8	10706.0
9	13049.0
10	15246.0
11	18287.0
12	21329.0
13	25177.0
14	28190.0
15	31501.0
16	35602.0
17	40495.0
18	42168.0
19	41008.0
20	42291.0
21	45130.0
22	48344.0
23	53478.0
24	60313.0
25	70351.0
26	83570.0
27	100368.0
28	121931.0
29	147784.0
30	180092.0
31	222918.0
32	281636.0
33	371777.0
34	511499.0
35	739583.0
36	1075643.0
37	1184301.0
38	443440.0
39	11545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84114297285606	17.256418230827535	13.224996844028247	24.677441952288156
2	16.291051392720675	19.08608873832488	37.9305899621754	26.692269906779043
3	21.447423518944348	21.98520009291133	27.17468375184959	29.392692636294736
4	14.251638145206632	14.448357094426381	34.366986983612755	36.93301777675423
5	15.804528879571823	35.660546527279266	32.50960648531389	16.025318107835027
6	35.85973358304126	32.75785670434106	15.94997070317369	15.432439009443986
7	31.951232733661872	29.712666965105644	18.97079410557823	19.365306195654256
8	28.23148056374723	33.05207221772413	18.461704808894115	20.25474240963452
9	26.785980601886273	16.79978261705449	18.86102726955791	37.55320951150133
10-11	27.328922332103524	24.44970749879594	25.589955794794722	22.631414374305812
12-13	27.602037783247457	21.458609910948944	26.195517176108456	24.743835129695142
14-15	24.25345482738951	23.138869340330444	24.118286296566417	28.489389535713627
16-17	24.71183537143434	26.23506477051345	24.508424572468304	24.544675285583907
18-19	24.625682246014257	26.052604116333526	25.51688934248231	23.804824295169897
20-21	25.4512833597898	24.674487362715368	25.311926665981744	24.56230261151309
22-23	24.95897105219995	24.45714432734395	25.44844808788862	25.13543653256748
24-25	25.166613217195366	24.629066162834718	25.410058206996815	24.7942624129731
26-27	24.9697430474121	24.55955394628153	25.889043156832297	24.58165984947407
28-29	24.661470041860195	25.389708676410542	25.496125683002564	24.45269559872669
30-31	24.93927784741908	24.82982888492982	25.231208723061414	24.99968454458968
32-33	24.828368425866525	24.91611918221246	25.32954757163164	24.925964820289376
34-35	25.230333501530573	24.785026083967985	25.46291347304903	24.52172694145241
36-37	25.052416116731145	24.68421525166126	25.663300487641692	24.600068143965903
38-39	24.89119113176174	24.959853293508957	25.564614052255585	24.584341522473714
40-41	24.981621969232624	25.04379980104702	25.326851619256235	24.647726610464122
42-43	24.775418108262247	25.060021385230556	25.042090518807235	25.122469987699958
44-45	24.95063250755835	24.614571044377172	25.250547007168866	25.184249440895616
46-47	25.217565011459254	25.00656364728173	25.283024631563546	24.492846709695467
48-49	25.153944026798158	24.93852646932287	25.217235944311795	24.690293559567177
50-51	24.430970690580033	25.172020901795417	25.64962052924488	24.747387878379676
52-53	24.961538715014537	25.344528081520085	24.954064261971702	24.73986894149368
54-55	24.810828727221406	24.809100731994853	25.24977166351448	25.130298877269258
56-57	25.037328481474642	24.820020378963857	25.24359355813848	24.899057581423026
58-59	25.60800414384345	24.73886593227838	24.940633173813577	24.7124967500646
60-61	25.088287448936182	24.950842021024062	25.529879411147487	24.430991118892265
62-63	24.672951779889857	25.112930604447005	25.40947040011652	24.80464721554662
64-65	24.725428363259923	25.401606756559623	25.149658390422314	24.723306489758144
66-67	25.07664233605985	24.916294613694568	24.902425789934032	25.104637260311552
68-69	24.7401034238597	25.06055575145946	25.142242989376555	25.057097835304276
70-71	25.141755862902244	24.819308698228486	25.158474631037684	24.880460807831586
72-73	25.15409965539818	24.625041322632967	25.416908385310883	24.803950636657966
74-75	24.60084747493542	25.594682222629984	25.04512935097199	24.759340951462608
76-77	24.891301299000258	24.884845147472685	25.020134165438034	25.20371938808902
78-79	24.85001331436696	24.96034039741395	25.295736981778177	24.89390930644091
80-81	25.144693323956034	24.996074702925558	25.115815357563008	24.7434166155554
82-83	25.059187804249433	24.749562231323544	25.44243536852558	24.748814595901447
84-85	24.823146612511263	25.15863831653517	25.21572721058294	24.80248786037063
86-87	24.85978414812986	25.248822489378707	25.15139996997185	24.73999339251958
88-89	24.629467667262897	25.103989129053634	24.942503359810345	25.324039843873123
90-91	24.892207213224513	24.78637795313609	25.046657900539326	25.274756933100072
92-93	25.263132164668257	25.161779816385405	25.095050491641306	24.48003752730503
94-95	25.168908600473895	24.912302558183647	25.088975408386677	24.82981343295578
96-97	24.546620614946864	24.915718617487144	25.69941391034028	24.83824685722571
98-99	24.855870215798216	25.270823817047468	24.948207157865227	24.92509880928909
100-101	25.297286305499807	24.33869620815788	25.191095114739053	25.172922371603264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	164.5
2	256.0
3	363.0
4	287.5
5	251.0
6	515.0
7	1267.0
8	1866.0
9	1758.5
10	1310.0
11	958.5
12	883.0
13	938.0
14	1024.0
15	1094.5
16	1172.0
17	1324.5
18	1463.0
19	1569.0
20	1689.0
21	1781.0
22	1909.0
23	2165.5
24	2440.5
25	2859.0
26	3578.5
27	4553.5
28	6103.0
29	8599.5
30	12408.5
31	17257.0
32	23414.5
33	31026.0
34	39897.0
35	50468.0
36	63285.0
37	77573.0
38	92226.5
39	107476.0
40	124814.5
41	142668.0
42	160736.5
43	179002.5
44	196531.0
45	215074.0
46	235340.0
47	271936.5
48	325936.0
49	333878.5
50	300764.5
51	288057.5
52	287750.5
53	287417.5
54	285938.0
55	278251.0
56	262068.0
57	242316.0
58	222803.5
59	200263.0
60	175573.5
61	149968.5
62	122454.5
63	93682.5
64	68890.0
65	51313.5
66	38882.0
67	29830.0
68	22022.5
69	16012.5
70	11933.0
71	9094.0
72	6630.5
73	4762.5
74	3512.0
75	2404.0
76	1560.0
77	903.5
78	496.5
79	273.5
80	152.5
81	82.5
82	50.0
83	22.5
84	10.0
85	6.0
86	5.0
87	3.5
88	1.5
89	1.0
90	1.5
91	1.5
92	1.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3025228616049126
2	0.0
3	0.005834748353572249
4	0.05368611432763446
5	4.8221060773324367E-4
6	0.06350713703846819
7	0.07321564394083083
8	0.10229294358714543
9	0.08983583622070329
10-11	0.09489101075844013
12-13	0.09432843171608468
14-15	0.0630811843349705
16-17	0.03471916375679355
18-19	0.03804641695015293
20-21	0.027277046710777148
22-23	0.04127722802196566
24-25	0.03340915827245157
26-27	0.02073505613252948
28-29	0.02374887243086225
30-31	0.0029495215506350073
32-33	0.005127506128896824
34-35	0.007667148662958575
36-37	0.01116317556902459
38-39	0.01666841334064579
40-41	0.02066272454136949
42-43	0.003729095366470418
44-45	0.014602944570855063
46-47	0.02383727770894668
48-49	0.0028048583683150338
50-51	0.0071849380552253305
52-53	0.0025235688471373086
54-55	0.004275600721901428
56-57	0.0036487269318482107
58-59	0.0011331949281731227
60-61	0.005360574589301225
62-63	0.015028897274352759
64-65	0.006919722220972047
66-67	0.03799015904591738
68-69	0.059962889071628854
70-71	0.012762507418006516
72-73	0.032083079101185144
74-75	0.05000524002193737
76-77	0.2887155645368174
78-79	0.07039471188559135
80-81	0.03332878983782936
82-83	0.02795214156160369
84-85	0.019674192795516344
86-87	0.1153929984305652
88-89	0.5085473437589411
90-91	0.1292002954986604
92-93	0.047296823775168986
94-95	0.00848690669610509
96-97	0.005971374692430001
98-99	0.01048808071819805
100-101	0.007924327653749638
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6221348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.96941419989446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.81721001341452	27.62309748534435
2	16.46060400608903	13.81683815023985
3	6.753507808145262	8.503222995068095
4	3.3123480451315572	5.56069228321348
5	1.9314425154031607	4.053075546612064
6	1.216280648752605	3.0627951798488655
7	0.8273228232881529	2.4305577973324604
8	0.5932309561774259	1.991804457280786
9	0.43851574073972044	1.656382388065097
>10	2.4011416529132905	18.271472748028064
>50	0.16866810007053626	4.8612190605136805
>100	0.07700179915735697	5.3444459254930905
>500	0.0015357293410540299	0.41667508067593656
>1k	8.446306545412027E-4	0.8728425338785121
>5k	2.303538146541547E-4	0.7094886918298883
>10k+	1.1517690732707735E-4	0.8253896765758091
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	28518	0.4583894037112214	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	11758	0.18899441085758262	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	10949	0.1759907981357095	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	9582	0.15401806810999802	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	9040	0.14530612979695076	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	6911	0.11108525033481491	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	6788	0.10910818684310859	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	6222	0.10001048004387474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6073686924441455E-5
2	0.0	0.0	0.0	0.0	1.6073686924441455E-5
3	0.0	1.6073686924441455E-5	0.0	0.0	1.6073686924441455E-5
4	0.0	1.6073686924441455E-5	0.0	0.0	1.6073686924441455E-5
5	0.0	1.6073686924441455E-5	0.0	0.0	1.6073686924441455E-5
6	0.0	1.6073686924441455E-5	0.0	0.0	1.6073686924441455E-5
7	0.0	1.6073686924441455E-5	0.0	0.0	1.6073686924441455E-5
8	0.0	4.8221060773324366E-5	0.0	0.0	1.6073686924441455E-5
9	0.0	4.8221060773324366E-5	0.0	1.6073686924441455E-5	3.214737384888291E-5
10-11	0.0	4.8221060773324366E-5	0.0	4.8221060773324366E-5	3.214737384888291E-5
12-13	0.0	4.8221060773324366E-5	0.0	8.036843462220728E-5	5.62579042355451E-5
14-15	0.0	5.62579042355451E-5	0.0	8.840527808442801E-5	7.233159115998655E-5
16-17	0.0	6.429474769776582E-5	0.0	1.7681055616885602E-4	9.644212154664873E-5
18-19	0.0	6.429474769776582E-5	0.0	2.089579300177389E-4	9.644212154664873E-5
20-21	0.0	6.429474769776582E-5	0.0	2.571789907910633E-4	1.2858949539553164E-4
22-23	0.0	6.429474769776582E-5	0.0	4.018421731110364E-4	1.3662633885775237E-4
24-25	0.0	6.429474769776582E-5	0.0	4.982842946576851E-4	1.446631823199731E-4
26-27	0.0	6.429474769776582E-5	0.0	6.349106335154374E-4	2.250316169421804E-4
28-29	0.0	6.429474769776582E-5	0.0	0.0012055265193331092	2.250316169421804E-4
30-31	0.0	6.429474769776582E-5	0.0	0.0026039372817595156	2.250316169421804E-4
32-33	0.0	6.429474769776582E-5	0.0	0.006582174795558776	2.250316169421804E-4
34-35	0.0	6.429474769776582E-5	0.0	0.011195322942873474	2.4110530386662184E-4
36-37	0.0	7.233159115998655E-5	0.0	0.01755246612149007	2.4110530386662184E-4
38-39	0.0	1.0447896500886947E-4	0.0	0.02749404148425711	2.571789907910633E-4
40-41	0.0	1.2858949539553164E-4	0.0	0.03894654341792164	2.571789907910633E-4
42-43	0.0	1.2858949539553164E-4	0.0	0.05031064007350176	2.571789907910633E-4
44-45	0.0	1.2858949539553164E-4	0.0	0.06226142630182398	2.571789907910633E-4
46-47	0.0	1.3662633885775237E-4	0.0	0.07564277066642149	2.6521583425328404E-4
48-49	0.0	1.446631823199731E-4	0.0	0.08769803585975258	2.893263646399462E-4
50-51	0.0	1.446631823199731E-4	0.0	0.1002274748173547	3.2147373848882913E-4
52-53	0.0	1.446631823199731E-4	0.0	0.11233096107145911	3.4558426887549133E-4
54-55	0.0	1.446631823199731E-4	0.0	0.12553549487988777	3.696947992621535E-4
56-57	0.0	1.446631823199731E-4	0.0	0.1413600396570004	3.696947992621535E-4
58-59	0.0	1.446631823199731E-4	0.0	0.15558525258513106	3.7773164272437423E-4
60-61	8.036843462220728E-6	1.7681055616885602E-4	0.0	0.1703408971817683	3.8576848618659493E-4
62-63	1.6073686924441455E-5	1.7681055616885602E-4	0.0	0.18513672599571668	3.8576848618659493E-4
64-65	1.6073686924441455E-5	1.7681055616885602E-4	0.0	0.1992494231153763	4.018421731110364E-4
66-67	1.6073686924441455E-5	2.089579300177389E-4	0.0	0.21494537839709338	4.018421731110364E-4
68-69	1.6073686924441455E-5	2.089579300177389E-4	0.0	0.2319433023196902	4.018421731110364E-4
70-71	1.6073686924441455E-5	2.3306846040440108E-4	0.0	0.24840275773031822	4.179158600354778E-4
72-73	1.6073686924441455E-5	2.6521583425328404E-4	0.0	0.26466129205439076	5.304316685065681E-4
74-75	1.6073686924441455E-5	2.7325267771550473E-4	0.0	0.2818440633766187	5.304316685065681E-4
76-77	1.6073686924441455E-5	2.893263646399462E-4	0.0	0.2989143188903755	5.304316685065681E-4
78-79	1.6073686924441455E-5	2.893263646399462E-4	0.0	0.3190707222936251	5.304316685065681E-4
80-81	1.6073686924441455E-5	2.893263646399462E-4	0.0	0.3394682310007413	5.384685119687888E-4
82-83	2.4110530386662183E-5	2.893263646399462E-4	0.0	0.3579770814942357	5.62579042355451E-4
84-85	3.214737384888291E-5	2.893263646399462E-4	0.0	0.37693599522161436	5.62579042355451E-4
86-87	3.214737384888291E-5	2.893263646399462E-4	0.0	0.39616012478324636	5.706158858176716E-4
88-89	3.214737384888291E-5	2.893263646399462E-4	0.0	0.41625223343879814	5.866895727421131E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	21305	0.0	26.511076	1
TACATGG	22110	0.0	24.964212	2
GTACCTG	6530	0.0	24.942335	1
ACATGGG	22535	0.0	23.608665	3
TACCTGG	6925	0.0	22.498552	2
GAGTACA	16110	0.0	21.816496	1
CATGGGG	17085	0.0	21.380892	4
AGTACAT	15495	0.0	20.999044	2
GTATAGC	1245	0.0	17.213427	1
AGAGTAC	17015	0.0	16.659132	10-11
ACCTGGG	8975	0.0	16.407343	3
GTATAGG	1550	0.0	16.284271	1
ATGGGGG	7975	0.0	16.252335	5
GTGTAGC	3035	0.0	15.220791	1
TAGACTG	2490	0.0	14.872332	5
CGTATAC	385	8.0806785E-8	14.805787	3
ATGGGGA	7925	0.0	14.737358	5
ACACCGT	1365	0.0	14.608912	6
TATACTG	2145	0.0	14.608326	5
TACACTG	4510	0.0	14.316806	5
>>END_MODULE