FastQCFastQC Report
Mon 15 Aug 2016
SRR2921441_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921441_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338845
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG80650.045.4316861
GTATCAA81900.041.928091
TACCTGG85600.041.5576672
GGTATCA46350.034.9577181
ACCTGGG103550.033.7779733
TCAACGC102650.033.002334
CAACGCA106150.031.82785
TATCAAC107650.031.6401142
ATCAACG107850.031.2836073
AACGCAG113950.029.9622846
TATAACG3650.026.3748262
GTACATG153900.025.9615921
ACGCAGA136800.024.7847757
TACATGG159550.024.3647672
CGCAGAG147350.023.0674488
CCTGGGG110150.022.4317654
ACATGGG166850.022.4193533
CATGGGG128600.019.8908044
GCAGAGT168700.019.7410019
GTGTAGC30100.018.351221
Produced by FastQC (version 0.11.5)