##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921441_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5338845
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.725734124141084	31.0	31.0	34.0	26.0	34.0
2	29.80445283577253	31.0	31.0	34.0	26.0	34.0
3	29.93262849923532	31.0	31.0	34.0	26.0	34.0
4	33.41769914653825	37.0	35.0	37.0	28.0	37.0
5	33.37556250462413	37.0	35.0	37.0	28.0	37.0
6	33.468613904318254	37.0	35.0	37.0	28.0	37.0
7	33.33300273748348	37.0	35.0	37.0	27.0	37.0
8	33.298551653026074	37.0	35.0	37.0	27.0	37.0
9	34.66966225840982	39.0	35.0	39.0	27.0	39.0
10-11	34.51232008046684	38.0	35.0	39.0	27.0	39.0
12-13	34.31636355803549	38.0	35.0	39.0	26.5	39.0
14-15	35.21721908015685	39.0	35.5	40.5	25.5	41.0
16-17	35.029078012191775	39.0	35.0	40.0	25.0	41.0
18-19	34.80999841726066	39.0	34.5	40.0	24.0	41.0
20-21	34.669115885552024	39.0	34.0	40.0	23.5	41.0
22-23	34.68895875418747	39.0	34.5	40.0	23.0	41.0
24-25	34.51874272431584	39.0	34.0	40.0	21.5	41.0
26-27	34.19907564276543	38.0	34.0	40.0	18.5	41.0
28-29	33.91526603600592	38.0	33.5	40.0	17.5	41.0
30-31	33.614350763133224	38.0	33.0	40.0	16.0	41.0
32-33	33.298352827250085	38.0	33.0	40.0	12.5	41.0
34-35	32.833372105764454	38.0	32.0	40.0	8.5	41.0
36-37	32.40362016503569	38.0	31.0	40.0	8.0	41.0
38-39	32.12398561861227	37.5	31.0	40.0	7.5	41.0
40-41	31.807576826073806	37.0	30.0	40.0	2.0	41.0
42-43	31.473816246772476	37.0	30.0	40.0	2.0	41.0
44-45	31.10616594038598	36.5	30.0	39.0	2.0	40.0
46-47	30.771307745401863	36.0	29.0	39.0	2.0	40.0
48-49	30.49982880192251	36.0	28.0	39.0	2.0	40.0
50-51	29.816099455968473	35.0	27.5	38.5	2.0	39.5
52-53	30.414102113846724	35.5	29.5	38.5	2.0	40.0
54-55	30.805971797270757	36.0	30.0	39.0	2.0	40.0
56-57	30.614640526181226	36.0	29.0	39.0	2.0	40.0
58-59	30.234856883839107	35.5	28.5	39.0	2.0	40.0
60-61	29.730830076542773	35.0	28.0	39.0	2.0	40.0
62-63	29.189242804389337	35.0	27.0	38.0	2.0	40.0
64-65	28.589687938121447	34.0	26.0	38.0	2.0	40.0
66-67	28.052807957526394	34.0	26.0	37.0	2.0	39.5
68-69	27.488165886067115	33.5	24.5	36.5	2.0	39.0
70-71	26.922763069540323	33.0	22.5	36.0	2.0	39.0
72-73	26.31620912388354	33.0	21.0	35.0	2.0	38.0
74-75	25.668914156526363	32.0	19.0	35.0	2.0	37.0
76-77	25.025909442960042	31.5	16.0	35.0	2.0	36.5
78-79	24.405212831614328	31.0	8.5	34.5	2.0	36.0
80-81	23.762199595605416	31.0	4.5	34.0	2.0	35.5
82-83	23.066041437801623	30.0	2.0	34.0	2.0	35.0
84-85	22.379454825903355	29.5	2.0	34.0	2.0	35.0
86-87	21.68218856325666	29.0	2.0	34.0	2.0	35.0
88-89	20.954615464580822	29.0	2.0	34.0	2.0	35.0
90-91	20.14295404717687	27.0	2.0	33.5	2.0	35.0
92-93	19.258733864721677	25.5	2.0	33.0	2.0	34.0
94-95	18.296483228113946	24.5	2.0	32.5	2.0	34.0
96-97	17.369385700465173	22.0	2.0	32.0	2.0	34.0
98	16.741138954212005	18.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	233675.0
3	39191.0
4	30029.0
5	25603.0
6	24131.0
7	25482.0
8	29090.0
9	33229.0
10	36616.0
11	40017.0
12	43471.0
13	45770.0
14	41108.0
15	41782.0
16	46135.0
17	52200.0
18	57848.0
19	58544.0
20	61911.0
21	66552.0
22	72836.0
23	79176.0
24	87870.0
25	97663.0
26	108521.0
27	121515.0
28	137414.0
29	157022.0
30	181712.0
31	214622.0
32	258724.0
33	321632.0
34	413788.0
35	539929.0
36	667617.0
37	614333.0
38	223775.0
39	8312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.841334838303254	16.532873786740343	12.805024145205882	24.820767229750523
2	16.285409623471736	18.169850003373238	39.01662631275159	26.52811406040344
3	20.770358592199557	22.020266991724192	26.83456307557194	30.374811340504316
4	13.922573385599277	14.150937031231376	33.953054356732316	37.97343522643703
5	15.854102662096542	35.05482705434873	32.232831155764394	16.85823912779034
6	35.90987028254145	32.119634403990936	16.65636419252344	15.314131120944175
7	32.49806845113111	29.2640100772226	18.5963697187856	19.64155175286069
8	28.80190703289572	33.322379834371105	18.18112840773095	19.69458472500223
9	27.315910440116976	16.415633958512586	18.379498211833354	37.88895738953709
10-11	27.277240367954185	24.564773913982602	25.443632790286774	22.714352927776435
12-13	27.27980659436341	21.450015880790012	26.314005414361237	24.95617211048534
14-15	24.228743585254726	23.079838317555403	23.738053682895327	28.953364414294548
16-17	24.924436393235172	26.149789647066978	24.18748223251832	24.73829172717953
18-19	24.91438972316845	25.373017544859184	25.63370908398503	24.078883647987332
20-21	25.34212927617658	24.66872036559778	25.67402052105463	24.315129837171014
22-23	25.14308498061919	24.322149845622906	25.572475859067694	24.96228931469021
24-25	24.829234969476772	24.59737147016173	25.65553687505922	24.91785668530228
26-27	24.90169720367222	24.77806940984952	25.590906453532064	24.729326932946194
28-29	24.884583423750914	24.82520075046201	25.57230695827137	24.71790886751571
30-31	25.029616950360285	24.832826582517857	25.42170770183148	24.715848765290378
32-33	24.88435886553925	24.79364285265932	25.448197812060553	24.873800469740875
34-35	25.009746973642265	24.848096730602677	25.511831312000016	24.630324983755045
36-37	25.084795829287472	24.77274830856049	25.4175227308244	24.72493313132764
38-39	25.052285976524747	24.84886029712735	25.38257715673	24.716276569617904
40-41	25.12519624172949	24.740371226332535	25.442790861187742	24.69164167075023
42-43	25.03847510687425	24.648101803691915	25.4202596700078	24.893163419426035
44-45	24.975083079157987	24.808976550835276	25.399214503780637	24.816725866226104
46-47	25.150869913406908	24.740142591345286	25.355645139878895	24.753342355368904
48-49	25.140839548021855	24.83969713860581	25.182769220457352	24.83669409291499
50-51	24.927678253744183	25.068475571044036	25.15083173892031	24.853014436291474
52-53	24.996211905622552	25.00049942537812	25.180066406613673	24.823222262385656
54-55	24.92435993498506	24.888713629982686	25.322206234975848	24.864720200056407
56-57	24.923319858871253	24.94938366812334	25.33632012534734	24.79097634765807
58-59	24.99135558460301	24.87223069221692	25.30228153258213	24.834132190597945
60-61	25.030390101474836	24.88250463207193	25.303253715708333	24.783851550744902
62-63	24.891718128236132	24.98354714434077	25.305956258615442	24.818778468807658
64-65	24.944229802410657	24.812854561986153	25.312332884836025	24.930582750767165
66-67	24.93384500668639	24.92334084953666	25.278937186553357	24.863876957223585
68-69	24.903247567205984	24.904735341841647	25.28306324887795	24.908953842074418
70-71	24.84725721781936	24.762198035432164	25.323138779825182	25.06740596692329
72-73	24.99073621191708	24.826763869476284	25.173471371460305	25.00902854714633
74-75	24.80816426905518	24.98562260990295	25.192188521475583	25.01402459956628
76-77	24.95369054396992	24.80066962998258	25.20196963499765	25.043670191049856
78-79	24.92391037941551	24.740828623941947	25.3083547809986	25.026906215643947
80-81	24.856314417895224	24.953182053859198	25.197769071356806	24.992734456888773
82-83	25.03338961552609	24.813878712580365	25.168227859977133	24.98450381191641
84-85	25.013179169349222	24.85723977584312	25.107465260988942	25.022115793818717
86-87	24.973791393793622	24.828562225590435	25.254842477433883	24.94280390318206
88-89	24.955216369603722	24.613019279097646	25.23586390368346	25.195900447615173
90-91	24.934225040913834	24.721035615146985	25.19956402480185	25.145175319137326
92-93	24.98509814900948	24.801092275572856	25.234007980769086	24.979801594648578
94-95	25.121587054599882	24.61753154968959	25.178745272215657	25.08213612349487
96-97	25.083076810757415	24.56707434618378	25.2170872965793	25.1327615464795
98	25.02424832086846	24.663124734115165	25.101184409350413	25.211442535665963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	644.0
1	489.0
2	640.0
3	916.5
4	692.5
5	537.5
6	872.5
7	1779.5
8	2539.0
9	2474.5
10	2038.0
11	1805.0
12	1993.5
13	2334.0
14	2729.0
15	3209.5
16	3582.5
17	3863.5
18	3989.0
19	3947.0
20	3805.0
21	3619.5
22	3518.0
23	3592.0
24	3890.5
25	4467.5
26	5498.0
27	7068.5
28	9151.0
29	11974.0
30	15571.0
31	20322.5
32	27058.5
33	35021.5
34	44111.0
35	54696.5
36	66525.0
37	79711.5
38	93304.5
39	106246.0
40	120117.0
41	135048.0
42	150430.5
43	166546.5
44	182119.5
45	197335.5
46	213268.5
47	229121.0
48	238979.5
49	243901.5
50	246565.0
51	248260.0
52	252510.5
53	255791.5
54	253962.0
55	246372.5
56	233364.0
57	218044.0
58	200768.5
59	179850.0
60	157184.0
61	133133.0
62	108605.5
63	84008.0
64	62566.0
65	47181.5
66	35837.5
67	27005.0
68	20273.5
69	15232.5
70	11313.0
71	8486.0
72	6467.0
73	4840.5
74	3619.5
75	2635.5
76	1782.0
77	1125.5
78	687.5
79	414.0
80	248.0
81	146.5
82	93.5
83	58.5
84	43.5
85	38.0
86	21.0
87	15.0
88	14.0
89	12.5
90	12.5
91	10.0
92	9.0
93	6.5
94	4.5
95	4.5
96	3.5
97	4.5
98	4.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4829134391427359
2	0.05104100231417095
3	0.05945106104410224
4	0.057259575807126824
5	0.06769254398657387
6	0.06662489733266277
7	0.04596499804733046
8	0.021146896004660184
9	0.01303652756354605
10-11	0.015799297413579154
12-13	0.011884593015905125
14-15	0.015930411914936656
16-17	0.014844034617974487
18-19	0.056884962946105386
20-21	0.08555221213577094
22-23	0.10256900134766977
24-25	0.07135438470305844
26-27	0.08781861994495063
28-29	0.08986026003751747
30-31	0.0602752093383494
32-33	0.03483899607499375
34-35	0.4085153249438783
36-37	0.637666012030692
38-39	0.585435613882778
40-41	0.5802378604361056
42-43	0.5538276537340941
44-45	0.5375413596011872
46-47	0.5271552180293678
48-49	0.516413194239578
50-51	0.5314726312526399
52-53	0.6133723679934517
54-55	0.5833096858964814
56-57	0.539442519870871
58-59	0.517199881247723
60-61	0.5303862539556777
62-63	0.5427484783693852
64-65	0.5620316753904637
66-67	0.588563631272307
68-69	0.5413343148190292
70-71	0.4883734215921234
72-73	0.470963288876152
74-75	0.4509683274191328
76-77	0.4657468047864285
78-79	0.45988411351144304
80-81	0.4565875203344544
82-83	0.47653565518384594
84-85	0.4427736710842889
86-87	0.44573311268635823
88-89	0.4727707959305805
90-91	0.4555760656096965
92-93	0.4508278475962498
94-95	0.461682255244346
96-97	0.45581956396936035
98	0.45035958151997296
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5338845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.91016972325983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45968056951502	42.18963547904556
2	13.447469536609477	15.037006082804044
3	4.6539453897623995	7.80608629873195
4	2.0773588348778067	4.645819401365259
5	1.151595985025477	3.219296348769951
6	0.7064798374882924	2.369964457201887
7	0.4688679794843745	1.8350141817541303
8	0.3336495008280771	1.4923520175502971
9	0.24996510025333726	1.257803206205017
>10	1.33172810698458	13.485254161553934
>50	0.08693775158620068	3.3109566535088035
>100	0.031579419973999566	2.8764547377840044
>500	6.070814089587149E-4	0.23504822315841642
>1k	1.011796515243478E-4	0.13901064606260063
>5k	3.372655050796721E-5	0.10029810450425403
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	1.8730643051071907E-5	0.0	0.0	0.0
8	0.0	1.8730643051071907E-5	0.0	0.0	0.0
9	0.0	1.8730643051071907E-5	0.0	0.0	0.0
10-11	0.0	1.8730643051071907E-5	0.0	3.746128610214381E-5	0.0
12-13	1.8730643051071907E-5	3.746128610214381E-5	0.0	5.619192915321572E-5	7.492257220428763E-5
14-15	1.8730643051071907E-5	3.746128610214381E-5	0.0	5.619192915321572E-5	1.030185367808955E-4
16-17	1.8730643051071907E-5	3.746128610214381E-5	0.0	5.619192915321572E-5	1.1238385830643145E-4
18-19	1.8730643051071907E-5	3.746128610214381E-5	0.0	5.619192915321572E-5	1.4984514440857528E-4
20-21	1.8730643051071907E-5	3.746128610214381E-5	0.0	1.030185367808955E-4	2.06037073561791E-4
22-23	1.8730643051071907E-5	3.746128610214381E-5	0.0	1.217491798319674E-4	2.1540239508732696E-4
24-25	1.8730643051071907E-5	4.682660762767977E-5	0.0	1.6857578745964718E-4	2.247677166128629E-4
26-27	1.8730643051071907E-5	5.619192915321572E-5	0.0	2.1540239508732693E-4	2.247677166128629E-4
28-29	1.8730643051071907E-5	5.619192915321572E-5	0.0	3.6524753949590224E-4	2.247677166128629E-4
30-31	1.8730643051071907E-5	5.619192915321572E-5	0.0	0.001198761155268602	2.3413303813839883E-4
32-33	1.8730643051071907E-5	5.619192915321572E-5	0.0	0.0027534045285075703	2.622290027150067E-4
34-35	1.8730643051071907E-5	5.619192915321572E-5	9.365321525535953E-6	0.004336143866323147	2.622290027150067E-4
36-37	1.8730643051071907E-5	7.492257220428763E-5	1.8730643051071907E-5	0.0072487588607648284	2.622290027150067E-4
38-39	2.8095964576607858E-5	9.365321525535954E-5	1.8730643051071907E-5	0.011772209157598693	2.622290027150067E-4
40-41	5.619192915321572E-5	9.365321525535954E-5	1.8730643051071907E-5	0.016660906993928463	2.622290027150067E-4
42-43	5.619192915321572E-5	9.365321525535954E-5	1.8730643051071907E-5	0.021521508865681622	2.622290027150067E-4
44-45	5.619192915321572E-5	9.365321525535954E-5	1.8730643051071907E-5	0.027093875173375515	2.8095964576607864E-4
46-47	5.619192915321572E-5	9.365321525535954E-5	1.8730643051071907E-5	0.03301275837751424	2.996902888171505E-4
48-49	5.619192915321572E-5	9.365321525535954E-5	1.8730643051071907E-5	0.03903466011843386	3.1842093186822243E-4
50-51	5.619192915321572E-5	1.030185367808955E-4	1.8730643051071907E-5	0.04568403840156439	3.1842093186822243E-4
52-53	9.365321525535954E-5	1.1238385830643145E-4	1.8730643051071907E-5	0.05176213207163721	3.558822179703662E-4
54-55	9.365321525535954E-5	1.4047982288303932E-4	1.8730643051071907E-5	0.05842087567629328	3.9334350407251007E-4
56-57	9.365321525535954E-5	1.5921046593411121E-4	1.8730643051071907E-5	0.06559471196485382	4.0270882559804603E-4
58-59	9.365321525535954E-5	1.9667175203625503E-4	1.8730643051071907E-5	0.07341475543867634	4.3080479017465386E-4
60-61	9.365321525535954E-5	2.06037073561791E-4	1.8730643051071907E-5	0.08163750773809691	4.5890075475126175E-4
62-63	9.365321525535954E-5	2.06037073561791E-4	1.8730643051071907E-5	0.0892889754244598	5.057273623789415E-4
64-65	9.365321525535954E-5	2.06037073561791E-4	1.8730643051071907E-5	0.09743680515167608	5.150926839044775E-4
66-67	9.365321525535954E-5	2.06037073561791E-4	1.8730643051071907E-5	0.10546288569906037	5.431886484810853E-4
68-69	9.365321525535954E-5	2.06037073561791E-4	1.8730643051071907E-5	0.11435994114831953	5.900152561087651E-4
70-71	9.365321525535954E-5	2.247677166128629E-4	1.8730643051071907E-5	0.12421225939318337	6.649378283130527E-4
72-73	1.030185367808955E-4	2.434983596639348E-4	1.8730643051071907E-5	0.1332872559514277	7.585910435684123E-4
74-75	1.1238385830643145E-4	2.434983596639348E-4	1.8730643051071907E-5	0.1419876396486506	8.054176511960921E-4
76-77	1.1238385830643145E-4	2.622290027150067E-4	1.8730643051071907E-5	0.1520178990024996	8.428789372982358E-4
78-79	1.1238385830643145E-4	2.8095964576607864E-4	1.8730643051071907E-5	0.1617016414599038	8.616095803493077E-4
80-81	1.3111450135750336E-4	2.8095964576607864E-4	1.8730643051071907E-5	0.1720222257810444	9.178015095025235E-4
82-83	1.3111450135750336E-4	2.8095964576607864E-4	1.8730643051071907E-5	0.18361649382965792	0.0010489160108600269
84-85	1.3111450135750336E-4	2.8095964576607864E-4	1.8730643051071907E-5	0.1949672635186075	0.0010489160108600269
86	1.3111450135750336E-4	2.8095964576607864E-4	1.8730643051071907E-5	0.203957972183122	0.0010676466539110987
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	8065	0.0	45.431686	1
GTATCAA	8190	0.0	41.92809	1
TACCTGG	8560	0.0	41.557667	2
GGTATCA	4635	0.0	34.957718	1
ACCTGGG	10355	0.0	33.777973	3
TCAACGC	10265	0.0	33.00233	4
CAACGCA	10615	0.0	31.8278	5
TATCAAC	10765	0.0	31.640114	2
ATCAACG	10785	0.0	31.283607	3
AACGCAG	11395	0.0	29.962284	6
TATAACG	365	0.0	26.374826	2
GTACATG	15390	0.0	25.961592	1
ACGCAGA	13680	0.0	24.784775	7
TACATGG	15955	0.0	24.364767	2
CGCAGAG	14735	0.0	23.067448	8
CCTGGGG	11015	0.0	22.431765	4
ACATGGG	16685	0.0	22.419353	3
CATGGGG	12860	0.0	19.890804	4
GCAGAGT	16870	0.0	19.741001	9
GTGTAGC	3010	0.0	18.35122	1
>>END_MODULE