FastQCFastQC Report
Mon 15 Aug 2016
SRR2921441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338845
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59500.11144732615387784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84200.044.6986161
GTACCTG84150.043.7061161
TACCTGG88600.040.9080732
GGTATCA43750.040.8349761
TCAACGC107350.034.560784
CAACGCA110500.033.5566755
ACCTGGG107450.032.6272473
TATCAAC116500.032.1713522
ATCAACG115450.032.1356853
AACGCAG120950.031.1685776
ACGCAGA146400.025.4269127
GTACATG161900.025.3946951
TACATGG164250.024.3225352
CGCAGAG155650.023.8548268
ACATGGG168500.022.7511863
CCTGGGG111500.022.5806034
TATCACG4900.020.3582972
GCAGAGT180150.020.1354549
TATAACG5300.019.71812
GAGTACA121250.019.567111
Produced by FastQC (version 0.11.5)