##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5338845
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8802703581018	33.0	31.0	34.0	30.0	34.0
2	31.993078090860475	34.0	31.0	34.0	30.0	34.0
3	32.11847056807231	34.0	31.0	34.0	30.0	34.0
4	35.677826571102926	37.0	35.0	37.0	35.0	37.0
5	35.475830821085836	37.0	35.0	37.0	33.0	37.0
6	35.515515059905276	37.0	35.0	37.0	33.0	37.0
7	35.44851348934086	37.0	35.0	37.0	33.0	37.0
8	35.398314429431835	37.0	35.0	37.0	33.0	37.0
9	37.01396594207174	39.0	37.0	39.0	33.0	39.0
10-11	36.91972083849596	39.0	37.0	39.0	33.0	39.0
12-13	36.79062709256402	39.0	37.0	39.0	33.0	39.0
14-15	38.0620945166979	40.0	38.0	41.0	33.0	41.0
16-17	37.95033963338513	40.0	38.0	41.0	33.0	41.0
18-19	37.83851029576622	40.0	38.0	41.0	33.0	41.0
20-21	37.71661595345061	40.0	38.0	41.0	32.0	41.0
22-23	37.67753774458708	40.0	38.0	41.0	32.5	41.0
24-25	37.73024661701173	40.0	38.0	41.0	32.5	41.0
26-27	37.610777799318015	40.0	38.0	41.0	32.0	41.0
28-29	37.46319166411462	40.0	37.5	41.0	32.0	41.0
30-31	37.31427237539205	40.0	37.0	41.0	32.0	41.0
32-33	37.14192582852658	40.0	37.0	41.0	31.0	41.0
34-35	36.982327357321665	40.0	37.0	41.0	31.0	41.0
36-37	36.87223650433755	40.0	36.5	41.0	30.5	41.0
38-39	36.83320774437168	40.0	36.5	41.0	31.0	41.0
40-41	36.65603946171878	39.5	36.0	41.0	30.0	41.0
42-43	36.39792492570959	39.0	36.0	41.0	30.0	41.0
44-45	36.173064211453976	39.0	35.0	40.0	30.0	41.0
46-47	35.871931382162245	39.0	35.0	40.0	28.5	41.0
48-49	35.507025770555245	38.0	35.0	40.0	28.0	41.0
50-51	35.199318672859015	38.0	34.5	40.0	27.0	41.0
52-53	34.854581093850825	38.0	34.0	40.0	26.0	41.0
54-55	34.494469683985955	38.0	34.0	40.0	26.0	41.0
56-57	34.075936649219074	37.0	33.0	40.0	24.5	41.0
58-59	33.64451655742019	37.0	33.0	39.5	23.0	41.0
60-61	33.182754603289666	36.0	32.0	39.0	22.5	40.0
62-63	32.70480328610402	35.5	31.5	39.0	21.5	40.0
64-65	32.14294383897641	35.0	31.0	38.0	19.5	40.0
66-67	31.573085938999913	35.0	30.5	38.0	18.0	40.0
68-69	31.028470296477984	34.5	30.0	37.0	14.0	39.0
70-71	30.438034443779507	34.0	29.0	36.5	9.5	39.0
72-73	29.854626609313435	34.0	29.0	36.0	7.5	38.5
74-75	29.299638311282685	34.0	28.0	35.0	7.0	37.5
76-77	28.110408337383834	31.5	27.0	34.0	4.5	36.0
78-79	29.039237138369817	33.5	29.0	35.0	2.0	36.0
80-81	29.0484321046968	34.0	29.0	35.0	2.0	36.0
82-83	28.674841187560233	34.0	29.0	35.0	2.0	36.0
84-85	28.13432231128643	33.0	27.5	35.0	2.0	35.5
86-87	27.554190091677135	33.0	26.5	35.0	2.0	35.0
88-89	26.964598616367397	32.5	25.5	34.5	2.0	35.0
90-91	26.420857975835595	32.0	24.5	34.0	2.0	35.0
92-93	25.902975081689018	32.0	24.0	34.0	2.0	35.0
94-95	25.31779982374465	32.0	21.5	34.0	2.0	35.0
96-97	24.44132204624783	31.0	15.5	34.0	2.0	35.0
98-99	23.312883966475894	31.0	2.0	34.0	2.0	35.0
100-101	19.753999132771227	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34663.0
3	17978.0
4	11145.0
5	8511.0
6	7562.0
7	7909.0
8	9022.0
9	11037.0
10	12956.0
11	15475.0
12	18619.0
13	21737.0
14	24529.0
15	28371.0
16	32103.0
17	37913.0
18	38983.0
19	35846.0
20	36167.0
21	38551.0
22	41535.0
23	45712.0
24	51705.0
25	60223.0
26	71070.0
27	85688.0
28	103461.0
29	125177.0
30	152762.0
31	189614.0
32	239083.0
33	313513.0
34	436610.0
35	632745.0
36	923584.0
37	1023868.0
38	383644.0
39	9774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06492249791671	17.109551371963967	13.26401252133785	24.56151360878147
2	16.544945582799276	18.44460740103899	38.655046175717786	26.355400840443956
3	20.773250459068407	22.162659414231896	27.13535882262698	29.928731304072713
4	14.19697369525843	14.491391434466047	33.848088018328156	37.46354685194736
5	15.867601504601577	35.05694701939866	32.321643238901764	16.753808237097996
6	35.969206495994406	32.30039822611604	16.45222832880166	15.278166949087895
7	32.444944804218615	29.348593806585704	18.558592704394595	19.647868684801082
8	28.78770264863087	33.48047324274935	18.109646545462184	19.622177563157596
9	27.174920949290932	16.45621473745941	18.40678840582101	37.96207590742865
10-11	27.211924458953003	24.666791470364107	25.491620794120866	22.629663276562027
12-13	27.2107478205928	21.456550361308896	26.40794228056994	24.924759537528363
14-15	24.126612069856606	23.089757489365166	23.83973656784556	28.94389387293267
16-17	24.807778535390014	26.212618347244852	24.383500067360657	24.59610305000447
18-19	24.817212424833176	25.416420264143504	25.762086465362366	24.004280845660954
20-21	25.196832469186216	24.660751177060273	25.886154788426115	24.256261565327396
22-23	25.00712533258579	24.286853053999057	25.821605657392272	24.884415956022877
24-25	24.680583443748873	24.556516440634507	25.900164988597137	24.862735127019484
26-27	24.77391758133005	24.72972260297744	25.858174586276267	24.638185229416244
28-29	24.72969586716009	24.861273027291066	25.77922184371957	24.629809261829276
30-31	24.860328262883783	24.830536246001703	25.658081864266595	24.65105362684792
32-33	24.737463849035425	24.7345510733672	25.711183213495875	24.8168018641015
34-35	24.82625234912781	24.8454153314161	25.771925856304794	24.556406463151298
36-37	24.870451228265196	24.858790082612426	25.66181684770478	24.608941841417597
38-39	24.87458990865003	24.82435562091519	25.652682776046216	24.64837169438857
40-41	24.936045281907525	24.7729712888981	25.68716546132358	24.603817967870796
42-43	24.85501920152668	24.74231255237759	25.649079409392435	24.753588836703294
44-45	24.77140846517641	24.805812288506175	25.670647412889625	24.752131833427796
46-47	24.843956073603106	24.82128636719735	25.63059488588285	24.704162673316695
48-49	24.906041928493806	24.923471315826962	25.410538869641012	24.759947886038216
50-51	24.794141274294006	25.095606283532213	25.32832449537369	24.781927946800096
52-53	24.899942926212603	25.0492675842803	25.285223770642418	24.765565718864675
54-55	24.87722212799244	24.88618514445114	25.408559484037447	24.828033243518977
56-57	24.86046082113735	25.03003571693486	25.398013053501977	24.71149040842581
58-59	24.932786578949955	24.959824604040907	25.36811095798617	24.73927785902297
60-61	24.997838826771872	24.90765491772041	25.390380938742467	24.704125316765243
62-63	24.791208770622593	25.045187402322668	25.392233770868465	24.771370056186274
64-65	24.899261857729073	24.87160411799674	25.41251757643272	24.816616447841465
66-67	24.84247718164173	25.02626824688695	25.32165310211717	24.80960146935415
68-69	24.83864861420357	24.988389222499606	25.372013434391143	24.80094872890568
70-71	24.784512170209368	24.87865476959978	25.42709090750712	24.909742152683727
72-73	24.97246167258779	24.910977051799783	25.21309765758865	24.90346361802377
74-75	24.73416498669689	25.12404290915326	25.224339798815908	24.917452305333942
76-77	24.868278373660765	24.970542472245384	25.27117224285948	24.89000691123437
78-79	24.81396071381877	24.911199096878015	25.420746968857006	24.85409322044621
80-81	24.736902901705726	25.106114322883023	25.332119304473512	24.82486347093774
82-83	24.96402939027627	25.003187447088614	25.22257561275567	24.810207549879447
84-85	24.87193953400931	25.056528327485587	25.194328273736954	24.87720386476815
86-87	24.82361936945067	25.087394498621446	25.308376023017033	24.78061010891085
88-89	24.794075916233076	24.889669993316698	25.340583319690264	24.97567077075996
90-91	24.79943465103055	24.979022597454428	25.2999665411836	24.921576210331423
92-93	24.883849887129376	25.091750718395385	25.300916495227497	24.723482899247742
94-95	24.96442513900023	24.925705603589694	25.232558390024106	24.877310867385965
96-97	24.860813796751614	24.915866384241962	25.296673317683588	24.926646501322836
98-99	24.815780967590957	24.935819665459565	25.289201378309507	24.95919798863997
100-101	25.418114423507582	24.310766590408306	25.38256090600403	24.888558080080085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	224.0
1	191.5
2	467.5
3	744.0
4	507.5
5	334.0
6	614.5
7	1513.5
8	2289.5
9	2187.5
10	1582.0
11	1084.0
12	1005.0
13	1071.5
14	1167.0
15	1306.0
16	1460.0
17	1659.0
18	1873.5
19	2000.5
20	2160.0
21	2354.0
22	2601.5
23	2941.0
24	3421.0
25	4128.0
26	4933.5
27	6192.5
28	7983.0
29	10372.0
30	13922.5
31	18286.5
32	23839.5
33	31125.0
34	38919.5
35	47750.5
36	59031.0
37	71691.0
38	84297.0
39	96054.5
40	108959.0
41	122680.5
42	136899.5
43	152227.0
44	168237.5
45	184210.5
46	199084.5
47	213175.5
48	227518.0
49	239185.0
50	243431.5
51	246512.0
52	250701.0
53	252879.5
54	252020.0
55	244912.0
56	231212.5
57	214625.0
58	197154.5
59	176830.5
60	155022.0
61	132015.0
62	107894.5
63	83260.0
64	61213.0
65	45684.0
66	34435.0
67	26216.5
68	19973.5
69	15023.5
70	11311.0
71	8421.0
72	6133.0
73	4517.5
74	3390.0
75	2483.5
76	1732.0
77	1068.0
78	636.0
79	355.5
80	185.0
81	104.0
82	62.5
83	29.0
84	17.5
85	14.0
86	8.5
87	3.5
88	1.0
89	1.0
90	1.0
91	1.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.313363658244433
2	0.0
3	0.005918883204138723
4	0.06048124641191119
5	4.3080479017465386E-4
6	0.06432102823738094
7	0.07524099313615586
8	0.10487287044295161
9	0.09228587831263128
10-11	0.09619121738877978
12-13	0.09576041259860513
14-15	0.06423674034365111
16-17	0.035625683083138765
18-19	0.03837908761164634
20-21	0.02812406054118447
22-23	0.042181408151013934
24-25	0.03389309860091462
26-27	0.021249914541441078
28-29	0.02420935614351044
30-31	0.003118652068003473
32-33	0.005516174378540677
34-35	0.00800734990433324
36-37	0.011322673724372968
38-39	0.016061526416294162
40-41	0.020304017067361946
42-43	0.003877243111571885
44-45	0.014525613686106265
46-47	0.02482746736419581
48-49	0.0030062682096970413
50-51	0.007763851544669307
52-53	0.0026597513132522108
54-55	0.004504719653782794
56-57	0.0037648592532654535
58-59	0.0012643184059473538
60-61	0.005797134024306756
62-63	0.014937687833229846
64-65	0.007033356465677501
66-67	0.03893164158165296
68-69	0.06153016242277122
70-71	0.012483973593539427
72-73	0.03255385762276297
74-75	0.05033860319975575
76-77	0.3062741098496023
78-79	0.07525035845768138
80-81	0.03361213895514854
82-83	0.02812406054118447
84-85	0.01937685023633389
86-87	0.11748795853784855
88-89	0.5215828517216738
90-91	0.1295598579842644
92-93	0.04958001215618734
94-95	0.008513077266712183
96-97	0.005862691274985507
98-99	0.010610909288432235
100-101	0.00775448622314377
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5338845.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.69483696903932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17699985557917	34.874345667993026
2	15.785989053076738	15.68964304775376
3	5.831066493867807	8.693216963051654
4	2.5877439560464532	5.143900560533814
5	1.48603411168369	3.6924111455276076
6	0.9182670023856726	2.7379877386562668
7	0.5870273441507726	2.042055971475855
8	0.43585424864591793	1.7327764662977616
9	0.30689944624969806	1.3726186152540472
>10	1.714725291080623	15.42784447215248
>50	0.12143575230951205	4.1205856398339815
>100	0.04701335581766725	3.900197600060136
>500	7.175084039437101E-4	0.25207221079902736
>1k	1.888172518814362E-4	0.20868331428470555
>5k	3.776345037310227E-5	0.11166058632594361
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5950	0.11144732615387784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8730643051071907E-5
2	0.0	0.0	0.0	0.0	1.8730643051071907E-5
3	0.0	0.0	0.0	0.0	1.8730643051071907E-5
4	0.0	0.0	0.0	0.0	1.8730643051071907E-5
5	0.0	0.0	0.0	0.0	1.8730643051071907E-5
6	0.0	0.0	0.0	0.0	3.746128610214381E-5
7	0.0	0.0	0.0	0.0	3.746128610214381E-5
8	0.0	0.0	0.0	0.0	3.746128610214381E-5
9	0.0	0.0	0.0	0.0	9.365321525535954E-5
10-11	0.0	0.0	9.365321525535953E-6	4.682660762767977E-5	1.3111450135750336E-4
12-13	0.0	9.365321525535953E-6	1.8730643051071907E-5	7.492257220428763E-5	3.1842093186822243E-4
14-15	0.0	1.8730643051071907E-5	1.8730643051071907E-5	7.492257220428763E-5	3.3715157491929435E-4
16-17	0.0	2.8095964576607858E-5	1.8730643051071907E-5	7.492257220428763E-5	3.558822179703663E-4
18-19	0.0	3.746128610214381E-5	1.8730643051071907E-5	8.428789372982357E-5	4.3080479017465386E-4
20-21	0.0	3.746128610214381E-5	1.8730643051071907E-5	1.6857578745964715E-4	5.150926839044775E-4
22-23	9.365321525535953E-6	3.746128610214381E-5	1.8730643051071907E-5	1.9667175203625503E-4	6.181112206853729E-4
24-25	1.8730643051071907E-5	3.746128610214381E-5	1.8730643051071907E-5	2.06037073561791E-4	6.181112206853729E-4
26-27	1.8730643051071907E-5	3.746128610214381E-5	2.8095964576607858E-5	2.434983596639348E-4	6.555725067875168E-4
28-29	1.8730643051071907E-5	3.746128610214381E-5	3.746128610214381E-5	3.839781825469741E-4	6.649378283130527E-4
30-31	1.8730643051071907E-5	3.746128610214381E-5	3.746128610214381E-5	0.0011238385830643145	6.836684713641247E-4
32-33	1.8730643051071907E-5	3.746128610214381E-5	3.746128610214381E-5	0.002594194062573459	7.492257220428763E-4
34-35	1.8730643051071907E-5	4.682660762767977E-5	6.555725067875168E-5	0.004111376149710284	8.24148294247164E-4
36-37	1.8730643051071907E-5	7.492257220428763E-5	7.492257220428763E-5	0.007117644359407325	8.428789372982358E-4
38-39	1.8730643051071907E-5	9.365321525535954E-5	7.492257220428763E-5	0.011603633370139046	8.428789372982358E-4
40-41	3.746128610214381E-5	9.365321525535954E-5	7.492257220428763E-5	0.016614080386300783	8.428789372982358E-4
42-43	3.746128610214381E-5	9.365321525535954E-5	7.492257220428763E-5	0.02134356775669644	8.428789372982358E-4
44-45	5.619192915321572E-5	9.365321525535954E-5	7.492257220428763E-5	0.026953395350492473	8.616095803493077E-4
46-47	6.555725067875168E-5	9.365321525535954E-5	7.492257220428763E-5	0.032535126979711906	9.365321525535954E-4
48-49	7.492257220428763E-5	1.1238385830643145E-4	7.492257220428763E-5	0.03825733843181437	0.001011454724757883
50-51	7.492257220428763E-5	1.3111450135750336E-4	7.492257220428763E-5	0.04471941028443418	0.001030185367808955
52-53	7.492257220428763E-5	1.3111450135750336E-4	7.492257220428763E-5	0.050638293488572905	0.0011332039045898505
54-55	7.492257220428763E-5	1.3111450135750336E-4	7.492257220428763E-5	0.05692242423220753	0.0011612998691664584
56-57	7.492257220428763E-5	1.3111450135750336E-4	7.492257220428763E-5	0.06348751462160823	0.0011893958337430661
58-59	7.492257220428763E-5	1.3111450135750336E-4	7.492257220428763E-5	0.07070817751779646	0.001217491798319674
60-61	7.492257220428763E-5	1.3111450135750336E-4	7.492257220428763E-5	0.07845329841941469	0.001217491798319674
62-63	7.492257220428763E-5	1.3111450135750336E-4	7.492257220428763E-5	0.08543046295593898	0.0013111450135750336
64-65	8.428789372982357E-5	1.3111450135750336E-4	7.492257220428763E-5	0.09303510403467416	0.0013111450135750336
66-67	9.365321525535954E-5	1.3111450135750336E-4	7.492257220428763E-5	0.10042434271832204	0.0013205103351005695
68-69	9.365321525535954E-5	1.4984514440857525E-4	7.492257220428763E-5	0.10893741998503421	0.0013486062996771774
70-71	9.365321525535954E-5	1.5921046593411121E-4	7.492257220428763E-5	0.11828401086751911	0.0013486062996771774
72-73	9.365321525535954E-5	1.6857578745964718E-4	7.492257220428763E-5	0.1266753389543993	0.0014235288718814649
74-75	9.365321525535954E-5	1.6857578745964718E-4	7.492257220428763E-5	0.1349823791475497	0.0014703554795091446
76-77	9.365321525535954E-5	1.6857578745964718E-4	7.492257220428763E-5	0.14445071920986655	0.0015171820871368246
78-79	9.365321525535954E-5	1.6857578745964718E-4	7.492257220428763E-5	0.15343206255285552	0.0015265474086623605
80-81	9.365321525535954E-5	1.6857578745964718E-4	7.492257220428763E-5	0.1630596130811065	0.0015452780517134323
82-83	9.365321525535954E-5	1.6857578745964718E-4	7.492257220428763E-5	0.17388592476462605	0.001629565945443256
84-85	9.365321525535954E-5	1.6857578745964718E-4	7.492257220428763E-5	0.18456239130373706	0.0016576619100198637
86-87	9.365321525535954E-5	2.06037073561791E-4	7.492257220428763E-5	0.1960817367801463	0.0017700457683262954
88-89	9.365321525535954E-5	2.247677166128629E-4	7.492257220428763E-5	0.20762917822113208	0.001863698983581655
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8420	0.0	44.698616	1
GTACCTG	8415	0.0	43.706116	1
TACCTGG	8860	0.0	40.908073	2
GGTATCA	4375	0.0	40.834976	1
TCAACGC	10735	0.0	34.56078	4
CAACGCA	11050	0.0	33.556675	5
ACCTGGG	10745	0.0	32.627247	3
TATCAAC	11650	0.0	32.171352	2
ATCAACG	11545	0.0	32.135685	3
AACGCAG	12095	0.0	31.168577	6
ACGCAGA	14640	0.0	25.426912	7
GTACATG	16190	0.0	25.394695	1
TACATGG	16425	0.0	24.322535	2
CGCAGAG	15565	0.0	23.854826	8
ACATGGG	16850	0.0	22.751186	3
CCTGGGG	11150	0.0	22.580603	4
TATCACG	490	0.0	20.358297	2
GCAGAGT	18015	0.0	20.135454	9
TATAACG	530	0.0	19.7181	2
GAGTACA	12125	0.0	19.56711	1
>>END_MODULE