FastQCFastQC Report
Mon 15 Aug 2016
SRR2921440_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921440_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5642764
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG71950.032.437931
GTATCAA109700.032.3536871
TACCTGG74200.031.0126172
TCAACGC134700.025.829084
CAACGCA138000.025.2114285
TATCAAC138100.025.1602022
GTACATG171350.024.7160871
ATCAACG140900.024.4975453
ACCTGGG92750.024.1181773
AACGCAG146300.023.9942686
TACATGG176650.023.1467382
ACATGGG184650.021.1509023
GAGTACA132600.020.795021
GGTATCA74450.020.7754571
AGTACAT124050.020.3237822
ACGCAGA172600.020.0687057
GTATAGG13050.019.0484331
CGCAGAG181750.019.0035698
CATGGGG148400.018.2861634
GTGTAGC33050.018.2463441
Produced by FastQC (version 0.11.5)