##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921440_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5642764 Sequences flagged as poor quality 0 Sequence length 98 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.105593464479465 31.0 31.0 34.0 26.0 34.0 2 30.194524172905336 31.0 31.0 34.0 26.0 34.0 3 30.329599111357485 31.0 31.0 34.0 26.0 34.0 4 33.83200608779669 37.0 35.0 37.0 30.0 37.0 5 33.804404898025155 37.0 35.0 37.0 30.0 37.0 6 33.902379755736725 37.0 35.0 37.0 30.0 37.0 7 33.77882133649396 37.0 35.0 37.0 29.0 37.0 8 33.75397517953967 37.0 35.0 37.0 29.0 37.0 9 35.174263534679106 39.0 35.0 39.0 29.0 39.0 10-11 35.033949054045145 39.0 35.0 39.0 28.5 39.0 12-13 34.85732142971069 38.5 35.0 39.0 27.0 39.0 14-15 35.828389420503854 39.5 36.0 41.0 27.0 41.0 16-17 35.66155469199137 39.0 36.0 41.0 27.0 41.0 18-19 35.458137891288736 39.0 36.0 40.0 27.0 41.0 20-21 35.32989329342854 39.0 35.5 40.0 26.0 41.0 22-23 35.36391163975669 39.0 36.0 40.0 26.5 41.0 24-25 35.21523210965406 39.0 35.0 40.5 25.0 41.0 26-27 34.92593354958669 39.0 35.0 40.0 24.0 41.0 28-29 34.674114317026195 39.0 34.0 40.0 23.5 41.0 30-31 34.40836901915444 38.0 34.0 40.0 22.5 41.0 32-33 34.12256723832505 38.0 34.0 40.0 19.5 41.0 34-35 33.68126967564123 38.0 33.0 40.0 16.5 41.0 36-37 33.27928529351928 38.0 33.0 40.0 15.0 41.0 38-39 33.02785310532214 38.0 32.0 40.0 10.5 41.0 40-41 32.74264748623193 38.0 32.0 40.0 9.0 41.0 42-43 32.43880968617508 37.5 31.0 40.0 8.5 41.0 44-45 32.10360649497303 37.0 31.0 40.0 7.5 41.0 46-47 31.800448414996623 37.0 30.0 40.0 6.5 41.0 48-49 31.574429481722078 37.0 30.0 39.0 2.0 40.0 50-51 30.85976110289213 35.5 29.0 38.5 2.0 39.5 52-53 31.412932740054345 36.5 30.5 39.0 2.0 40.0 54-55 31.824177743389587 37.0 31.0 39.5 2.0 40.5 56-57 31.645988207197746 36.5 31.0 39.5 2.0 41.0 58-59 31.288753437145342 36.0 30.5 39.0 2.0 40.5 60-61 30.805624335875116 35.5 30.0 39.0 2.0 40.0 62-63 30.28899578291773 35.0 29.0 38.5 2.0 40.0 64-65 29.71331673626613 35.0 28.0 38.0 2.0 40.0 66-67 29.196371671755188 34.0 28.0 37.5 2.0 40.0 68-69 28.649432264046485 34.0 26.5 37.0 2.0 39.0 70-71 28.100347365227396 34.0 26.0 36.0 2.0 39.0 72-73 27.517082762986366 33.5 25.5 35.5 2.0 38.5 74-75 26.895828976721337 33.0 24.5 35.0 2.0 37.5 76-77 26.279725769144342 32.5 22.5 35.0 2.0 37.0 78-79 25.685086776622235 32.0 20.5 35.0 2.0 36.0 80-81 25.07224686341658 31.5 18.5 34.5 2.0 36.0 82-83 24.408975282326182 31.0 14.0 34.0 2.0 35.0 84-85 23.75450913772045 31.0 7.0 34.0 2.0 35.0 86-87 23.09309595439398 30.0 2.0 34.0 2.0 35.0 88-89 22.397790692646367 29.5 2.0 34.0 2.0 35.0 90-91 21.61480189495786 29.0 2.0 34.0 2.0 35.0 92-93 20.750067254274676 29.0 2.0 34.0 2.0 35.0 94-95 19.79846029357244 27.0 2.0 33.0 2.0 34.0 96-97 18.873966818389 25.5 2.0 33.0 2.0 34.0 98 18.241935512454535 25.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 204599.0 3 35150.0 4 26971.0 5 22903.0 6 21774.0 7 22594.0 8 25538.0 9 29666.0 10 32951.0 11 36268.0 12 39540.0 13 42740.0 14 37094.0 15 37102.0 16 40302.0 17 45453.0 18 50584.0 19 53014.0 20 55873.0 21 60613.0 22 66837.0 23 73951.0 24 81559.0 25 91823.0 26 103009.0 27 116875.0 28 133342.0 29 155137.0 30 182802.0 31 218682.0 32 267996.0 33 339972.0 34 446649.0 35 605895.0 36 782856.0 37 758739.0 38 286124.0 39 9787.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.96931312428614 16.382992327479606 12.59371583195793 25.053978716276326 2 16.513107850525287 18.142169422403477 38.565982534686825 26.778740192384415 3 20.987671834818688 21.411005869186894 26.752129083099586 30.849193212894832 4 13.989949321029469 13.949111788091685 33.73963989204564 38.321298998833214 5 16.042551531262802 35.10884388727409 32.237103218586896 16.61150136287621 6 36.50748217307822 31.95743848472805 16.286222448274927 15.248856893918797 7 32.895986360448134 29.155109477403464 18.272368347331984 19.67653581481642 8 28.880173260052093 33.17499967207082 18.07951555820105 19.865311509676037 9 27.20543272773936 16.355255643700747 18.231059664789896 38.20825196377 10-11 27.32797811758131 24.43467626716665 25.44927456084634 22.788071054405698 12-13 27.40133499739057 21.2826013883115 26.191292554360057 25.124771059937874 14-15 24.291994344693133 23.011503194780442 23.647100414487944 29.04940204603848 16-17 25.062362052180553 26.111609617585714 24.12886547459367 24.69716285564006 18-19 25.034912656517356 25.46813015959679 25.411670557869275 24.08528662601658 20-21 25.441060850154116 24.673014065470355 25.452208929371995 24.43371615500353 22-23 25.229661830915102 24.314979546782673 25.34154336014658 25.113815262155644 24-25 25.01805422937171 24.523292084089434 25.434986329862475 25.023667356676377 26-27 25.00875593991523 24.741496893670117 25.44502302974057 24.804724136674082 28-29 24.965611854444553 24.864671372255746 25.361249586583934 24.80846718671577 30-31 25.10812838348676 24.81261012665613 25.219384401689908 24.859877088167202 32-33 25.00250856546576 24.79636298782734 25.178657727782177 25.022470718924723 34-35 25.10339017256789 24.819496544406608 25.300551378660213 24.77656190436529 36-37 25.21905903955198 24.702738241339254 25.266511388073067 24.8116913310357 38-39 25.139448759077652 24.843256652933814 25.231582556428943 24.78571203155959 40-41 25.254006092687508 24.747571723326306 25.223082681659665 24.77533950232652 42-43 25.13819035357499 24.6471330364039 25.187275588552733 25.027401021468375 44-45 25.08354853647164 24.72575132202578 25.236295763467492 24.954404378035086 46-47 25.20148414926024 24.741086408947517 25.221521197753997 24.835908244038244 48-49 25.21256856191879 24.82075308070412 25.0490916300185 24.917586727358586 50-51 24.924402041082523 25.01199232206281 25.15328076431541 24.910324872539256 52-53 25.114563903646918 24.95035913439635 25.05301609668659 24.88206086527014 54-55 25.025816656807127 24.753137133873274 25.22262409200429 24.99842211731531 56-57 25.02451503010816 24.90535483105814 25.23531978514631 24.834810353687395 58-59 25.161077669876963 24.80389440666778 25.163803647643324 24.871224275811937 60-61 25.14541655310581 24.787337896794405 25.22438274198085 24.84286280811893 62-63 24.906465243886302 24.953576802756725 25.202340447353972 24.937617506003004 64-65 25.016887036664855 24.800454896082 25.193426644664573 24.989231422588567 66-67 25.06342132083236 24.846626313803775 25.123267726054127 24.966684639309737 68-69 24.94008973663721 24.89044000250209 25.20119636968843 24.968273891172274 70-71 24.9773103480398 24.69448119802431 25.25306832401687 25.07514012991902 72-73 25.106473763302933 24.715322756789625 25.150077452867812 25.028126027039633 74-75 24.85833327250096 25.01375850236276 25.13181188303733 24.99609634209895 76-77 25.080395815054814 24.79664430832888 25.01906723937537 25.103892637240943 78-79 24.9916778155893 24.724922758821716 25.250704970705645 25.03269445488334 80-81 24.98270625055309 24.859375331074034 25.174758123432767 24.983160294940113 82-83 25.16055152074258 24.73589586501918 25.105449312736194 24.99810330150204 84-85 25.07540233196004 24.819778123135972 25.01375058315914 25.09106896174485 86-87 25.05282769953595 24.803710980096476 25.195096179438814 24.94836514092876 88-89 24.986583381379685 24.609724878066157 25.162844848503735 25.240846892050424 90-91 24.984540382408532 24.693135716123496 25.129085358633752 25.193238542834223 92-93 25.112751339208693 24.768697511271572 25.18453011209881 24.934021037420926 94-95 25.221283887705297 24.59553595809199 25.126544281840225 25.056635872362488 96-97 25.084142155974654 24.56846792235267 25.210409919306326 25.136980002366354 98 25.08806139584968 24.69803451807306 25.011341510140774 25.202562575936483 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 831.0 1 616.0 2 737.0 3 1042.0 4 801.5 5 658.0 6 1088.5 7 2114.0 8 3048.0 9 2924.0 10 2214.5 11 1896.5 12 2074.0 13 2468.0 14 2920.5 15 3394.5 16 3764.0 17 3916.5 18 3965.5 19 3880.5 20 3502.0 21 3119.0 22 2882.5 23 2717.5 24 2731.5 25 2937.5 26 3353.5 27 4056.0 28 5030.0 29 6677.5 30 9373.0 31 13453.5 32 19672.5 33 27548.0 34 37356.5 35 49545.0 36 63218.5 37 78134.0 38 94219.5 39 110582.0 40 127671.5 41 145458.5 42 163997.0 43 183362.5 44 201752.5 45 220341.0 46 241065.0 47 259839.5 48 269974.0 49 271442.5 50 270591.0 51 271939.5 52 274193.5 53 274538.0 54 269938.0 55 259480.0 56 244443.0 57 227245.5 58 208570.5 59 186759.0 60 163429.5 61 137925.0 62 111800.0 63 86533.0 64 63928.5 65 47910.0 66 36275.5 67 27607.5 68 21067.5 69 15799.0 70 11789.5 71 8954.0 72 6837.0 73 5029.5 74 3806.0 75 2900.0 76 1988.0 77 1203.0 78 729.5 79 416.0 80 241.5 81 154.5 82 105.0 83 69.0 84 37.5 85 26.0 86 22.5 87 20.0 88 19.0 89 16.0 90 12.5 91 10.5 92 7.5 93 6.0 94 8.0 95 8.5 96 7.5 97 3.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4971145346500403 2 0.05341353988931666 3 0.06170734767571353 4 0.059261737687417014 5 0.070072042708148 6 0.07019609538871376 7 0.047778003829329035 8 0.02307379858523234 9 0.014354667322609982 10-11 0.017269905315905467 12-13 0.01294578330761308 14-15 0.017429401619490022 16-17 0.01615343119081358 18-19 0.05907565866656837 20-21 0.0889900764944272 22-23 0.10629542543335146 24-25 0.0743607210934216 26-27 0.0914888519172519 28-29 0.0936331911098887 30-31 0.06235419379580645 32-33 0.03703858605463564 34-35 0.42103479784020736 36-37 0.6581969403646865 38-39 0.6038352835596172 40-41 0.5980225293845356 42-43 0.5691448375299765 44-45 0.553017989056427 46-47 0.5433507408780519 48-49 0.5324606877055287 50-51 0.546540666949743 52-53 0.6333864042515335 54-55 0.6022225987122624 56-57 0.5566509604158529 58-59 0.5334088046212814 60-61 0.5470457385777608 62-63 0.5602662099637695 64-65 0.579352600959388 66-67 0.6067948260816862 68-69 0.557439581028021 70-71 0.5036627440027618 72-73 0.4858168798128009 74-75 0.46496716857199766 76-77 0.48045603183120894 78-79 0.4740673187820721 80-81 0.47092169723915445 82-83 0.491673938516656 84-85 0.4559733492309797 86-87 0.4593316325120101 88-89 0.48781944451336257 90-91 0.4699647194176471 92-93 0.4643291833576595 94-95 0.4757331690639552 96-97 0.4700621893809488 98 0.46480767226841313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 5642764.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.209597501839745 #Duplication Level Percentage of deduplicated Percentage of total 1 74.25530766096419 39.510950330151815 2 13.84599006742144 14.734791170039314 3 4.846610132148004 7.736585231398008 4 2.2371376280315944 4.761487709751265 5 1.2228614050833064 3.253398158250847 6 0.794405619328889 2.536200195461394 7 0.5115943483456428 1.9055210551781352 8 0.36873993769675256 1.5696402934158133 9 0.2733573317455036 1.3090710236719614 >10 1.4951949081882063 14.520220572372097 >50 0.10521234968073807 3.812851093623104 >100 0.04241726035198236 3.6993918348900383 >500 9.036157305701364E-4 0.32427921376575886 >1k 2.342683727990149E-4 0.23504262216135552 >5k 3.346691038263225E-5 0.09056949586923757 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.772181150939504E-5 2 0.0 0.0 0.0 0.0 1.772181150939504E-5 3 0.0 0.0 0.0 0.0 1.772181150939504E-5 4 0.0 0.0 0.0 0.0 1.772181150939504E-5 5 0.0 1.772181150939504E-5 0.0 0.0 1.772181150939504E-5 6 0.0 1.772181150939504E-5 0.0 0.0 1.772181150939504E-5 7 0.0 3.544362301879008E-5 0.0 0.0 1.772181150939504E-5 8 0.0 3.544362301879008E-5 0.0 0.0 1.772181150939504E-5 9 0.0 3.544362301879008E-5 0.0 1.772181150939504E-5 1.772181150939504E-5 10-11 0.0 3.544362301879008E-5 0.0 7.088724603758016E-5 1.772181150939504E-5 12-13 1.772181150939504E-5 3.544362301879008E-5 0.0 1.240526805657653E-4 1.5063539782985785E-4 14-15 3.544362301879008E-5 4.43045287734876E-5 0.0 1.329135863204628E-4 1.683572093392529E-4 16-17 7.974815179227769E-5 5.3165434528185125E-5 0.0 1.5063539782985785E-4 2.126617381127405E-4 18-19 1.0633086905637025E-4 5.3165434528185125E-5 0.0 1.5949630358455538E-4 2.126617381127405E-4 20-21 1.0633086905637025E-4 5.3165434528185125E-5 0.0 1.8607902084864791E-4 2.3924445537683306E-4 22-23 1.0633086905637025E-4 5.3165434528185125E-5 0.0 2.9240988990501815E-4 3.1899260716911076E-4 24-25 1.329135863204628E-4 5.3165434528185125E-5 0.0 3.189926071691107E-4 3.3671441867850577E-4 26-27 1.4177449207516032E-4 5.3165434528185125E-5 0.0 4.341843819801785E-4 3.3671441867850577E-4 28-29 1.4177449207516032E-4 5.3165434528185125E-5 0.0 7.886206121680793E-4 3.3671441867850577E-4 30-31 1.4177449207516032E-4 5.3165434528185125E-5 0.0 0.002436749082541818 3.3671441867850577E-4 32-33 1.4177449207516032E-4 5.3165434528185125E-5 0.0 0.005803893269326876 3.721580416972959E-4 34-35 1.4177449207516032E-4 5.3165434528185125E-5 0.0 0.009702691801393784 3.721580416972959E-4 36-37 1.4177449207516032E-4 5.3165434528185125E-5 0.0 0.015559750505248847 3.721580416972959E-4 38-39 1.4177449207516032E-4 5.3165434528185125E-5 0.0 0.023880141008909818 4.0760166471608594E-4 40-41 1.4177449207516032E-4 5.3165434528185125E-5 0.0 0.033219535674361006 4.0760166471608594E-4 42-43 1.4177449207516032E-4 7.974815179227769E-5 0.0 0.042505764905284 4.5190619348957356E-4 44-45 1.4177449207516032E-4 1.0633086905637025E-4 0.0 0.05311226909365694 4.962107222630612E-4 46-47 1.5949630358455535E-4 1.1519177481106777E-4 0.0 0.06440992393089627 5.493761567912463E-4 48-49 1.772181150939504E-4 1.240526805657653E-4 0.0 0.07584935326021078 6.025415913194314E-4 50-51 1.772181150939504E-4 1.240526805657653E-4 0.0 0.08746600070461923 6.202634028288264E-4 52-53 1.772181150939504E-4 1.240526805657653E-4 0.0 0.09922442264110283 7.177333661304991E-4 54-55 1.772181150939504E-4 1.4177449207516032E-4 0.0 0.11170057794371693 7.265942718851967E-4 56-57 1.772181150939504E-4 1.5949630358455535E-4 0.0 0.12452230857076425 7.265942718851967E-4 58-59 1.772181150939504E-4 1.9493992660334544E-4 0.0 0.1379643026006404 7.354551776398942E-4 60-61 2.126617381127405E-4 1.9493992660334544E-4 0.0 0.15106072130608333 7.620378949039868E-4 62-63 2.126617381127405E-4 1.9493992660334544E-4 0.0 0.16462676801652523 8.152033294321719E-4 64-65 2.126617381127405E-4 1.9493992660334544E-4 0.0 0.17842319827658926 8.32925140941567E-4 66-67 2.126617381127405E-4 1.9493992660334544E-4 0.0 0.19367281708042372 8.68368763960357E-4 68-69 2.3038354962213553E-4 1.9493992660334544E-4 0.0 0.2094186466065212 8.86090575469752E-4 70-71 2.3038354962213553E-4 1.9493992660334544E-4 0.0 0.22629867206921997 9.835605387714247E-4 72-73 2.3038354962213553E-4 1.9493992660334544E-4 8.86090575469752E-6 0.24246096416578827 0.0011164741250918875 74-75 2.3038354962213553E-4 1.9493992660334544E-4 1.772181150939504E-5 0.25936757234575114 0.0011785004653747703 76-77 2.3038354962213553E-4 1.9493992660334544E-4 1.772181150939504E-5 0.27603493607033713 0.0012050831826388628 78-79 2.481053611315306E-4 1.9493992660334544E-4 1.772181150939504E-5 0.29287951791001715 0.0012050831826388628 80-81 2.658271726409256E-4 2.126617381127405E-4 1.772181150939504E-5 0.3109380438380907 0.0013025531459405356 82-83 2.658271726409256E-4 2.126617381127405E-4 1.772181150939504E-5 0.32884593436833437 0.0013291358632046282 84-85 2.8354898415032065E-4 2.126617381127405E-4 1.772181150939504E-5 0.3477019418143307 0.0013291358632046282 86 3.012707956597157E-4 2.126617381127405E-4 1.772181150939504E-5 0.3619857218909031 0.0013291358632046282 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 7195 0.0 32.43793 1 GTATCAA 10970 0.0 32.353687 1 TACCTGG 7420 0.0 31.012617 2 TCAACGC 13470 0.0 25.82908 4 CAACGCA 13800 0.0 25.211428 5 TATCAAC 13810 0.0 25.160202 2 GTACATG 17135 0.0 24.716087 1 ATCAACG 14090 0.0 24.497545 3 ACCTGGG 9275 0.0 24.118177 3 AACGCAG 14630 0.0 23.994268 6 TACATGG 17665 0.0 23.146738 2 ACATGGG 18465 0.0 21.150902 3 GAGTACA 13260 0.0 20.79502 1 GGTATCA 7445 0.0 20.775457 1 AGTACAT 12405 0.0 20.323782 2 ACGCAGA 17260 0.0 20.068705 7 GTATAGG 1305 0.0 19.048433 1 CGCAGAG 18175 0.0 19.003569 8 CATGGGG 14840 0.0 18.286163 4 GTGTAGC 3305 0.0 18.246344 1 >>END_MODULE