FastQCFastQC Report
Mon 15 Aug 2016
SRR2921440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5642764
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG73500.034.9937361
TACCTGG74300.032.730812
GTATCAA114350.032.489451
TCAACGC139100.026.055074
GTACATG177050.025.5032961
CAACGCA142400.025.4383965
TATCAAC145350.025.1623552
ATCAACG148200.024.5509193
ACCTGGG97100.024.3122023
TACATGG181650.023.847072
AACGCAG153850.023.7313966
GGTATCA75850.023.6738931
ACATGGG186850.022.3197123
ACGCAGA177050.020.434557
GAGTACA133150.020.1038421
AGAGTAC149100.019.12142210-11
CGCAGAG190150.019.0269188
CATGGGG148250.018.9680144
AGTACAT127400.018.8649942
TAACGCA4600.017.55444
Produced by FastQC (version 0.11.5)