##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5642764
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91557807485835	33.0	31.0	34.0	30.0	34.0
2	32.02939109273399	34.0	31.0	34.0	30.0	34.0
3	32.16029183570321	34.0	31.0	34.0	30.0	34.0
4	35.698179119311035	37.0	35.0	37.0	35.0	37.0
5	35.500718619456705	37.0	35.0	37.0	33.0	37.0
6	35.53566833558873	37.0	35.0	37.0	33.0	37.0
7	35.4673576637265	37.0	35.0	37.0	33.0	37.0
8	35.41837758942249	37.0	35.0	37.0	33.0	37.0
9	37.04037134992709	39.0	37.0	39.0	34.0	39.0
10-11	36.950967203306746	39.0	37.0	39.0	33.0	39.0
12-13	36.82622957472614	39.0	37.0	39.0	33.0	39.0
14-15	38.118923279442484	40.0	38.0	41.0	33.0	41.0
16-17	38.01439675662495	40.0	38.0	41.0	33.0	41.0
18-19	37.89954524059486	40.0	38.0	41.0	33.0	41.0
20-21	37.78327571381685	40.0	38.0	41.0	33.0	41.0
22-23	37.74870170008882	40.0	38.0	41.0	32.5	41.0
24-25	37.802805770363605	40.0	38.0	41.0	33.0	41.0
26-27	37.69229459179934	40.0	38.0	41.0	32.5	41.0
28-29	37.554959767943515	40.0	38.0	41.0	32.0	41.0
30-31	37.42110276098735	40.0	37.5	41.0	32.0	41.0
32-33	37.26502588093353	40.0	37.0	41.0	31.5	41.0
34-35	37.118491221677886	40.0	37.0	41.0	31.0	41.0
36-37	37.01829449184832	40.0	37.0	41.0	31.0	41.0
38-39	36.98324526774468	40.0	37.0	41.0	31.0	41.0
40-41	36.82315094163073	40.0	36.5	41.0	30.5	41.0
42-43	36.58921097887489	39.5	36.0	41.0	30.0	41.0
44-45	36.38472413519332	39.0	36.0	41.0	30.0	41.0
46-47	36.116836004482906	39.0	35.0	40.0	29.5	41.0
48-49	35.78390474951637	39.0	35.0	40.0	28.5	41.0
50-51	35.50091798983618	38.5	35.0	40.0	28.0	41.0
52-53	35.18065614652677	38.0	34.0	40.0	27.0	41.0
54-55	34.83607607902794	38.0	34.0	40.0	26.5	41.0
56-57	34.43168472046678	37.5	34.0	40.0	26.0	41.0
58-59	34.02168201611834	37.0	33.0	40.0	25.0	41.0
60-61	33.572049708263535	36.5	33.0	39.0	23.5	40.5
62-63	33.11094766678174	36.0	32.0	39.0	22.5	40.0
64-65	32.57530414172912	35.0	31.5	38.5	22.0	40.0
66-67	32.02292723920405	35.0	31.0	38.0	20.0	40.0
68-69	31.4961131636907	35.0	31.0	37.0	18.5	39.0
70-71	30.92011140285151	34.0	30.0	36.5	16.5	39.0
72-73	30.354758235503027	34.0	29.0	36.0	12.5	39.0
74-75	29.819395158117544	34.0	29.0	35.5	8.0	37.5
76-77	28.572941026773403	32.0	27.5	34.5	7.5	36.0
78-79	29.50675493428398	34.0	29.0	35.0	7.0	36.5
80-81	29.530591922681865	34.0	29.0	35.0	7.0	36.0
82-83	29.18167639121537	34.0	29.0	35.0	2.0	36.0
84-85	28.673709373633205	33.5	29.0	35.0	2.0	35.5
86-87	28.138171116140953	33.0	27.5	35.0	2.0	35.0
88-89	27.577141273319246	33.0	26.5	35.0	2.0	35.0
90-91	27.074859501478354	32.5	25.5	34.5	2.0	35.0
92-93	26.606604139389844	32.0	25.0	34.0	2.0	35.0
94-95	26.07175889333667	32.0	24.5	34.0	2.0	35.0
96-97	25.255782715704573	32.0	21.5	34.0	2.0	35.0
98-99	24.18853925133144	31.0	10.0	34.0	2.0	35.0
100-101	20.585286572325195	27.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37404.0
3	19512.0
4	11892.0
5	8878.0
6	7713.0
7	7972.0
8	9155.0
9	11156.0
10	13221.0
11	15493.0
12	17858.0
13	20999.0
14	23425.0
15	25848.0
16	29327.0
17	33194.0
18	34633.0
19	33662.0
20	34626.0
21	36195.0
22	39722.0
23	43462.0
24	49202.0
25	57874.0
26	68671.0
27	82840.0
28	100548.0
29	122450.0
30	150162.0
31	186525.0
32	238813.0
33	315928.0
34	445802.0
35	664004.0
36	1000898.0
37	1173604.0
38	458692.0
39	11404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32024539004696	16.79540369258505	12.950023246448804	24.93432767091918
2	16.651325485170034	18.36869307311098	38.22619553112624	26.753785910592754
3	20.99971129557396	21.49981329029292	27.024683785356324	30.475791628776793
4	14.236953492087784	14.22579940987989	33.70467501455439	37.832572083477935
5	16.101893160301373	35.02197693529413	32.36445311793893	16.511676786465568
6	36.62639915605095	32.10091922564235	16.10929771458839	15.163383903718309
7	32.89900160039045	29.176018830125933	18.308810802068024	19.616168767415594
8	28.88269002806163	33.30086264063192	18.065141732710906	19.751305598595533
9	27.023912805557394	16.36515280330821	18.2620569330214	38.34887745811299
10-11	27.31014166307973	24.47104399767657	25.500037295862803	22.718777043380896
12-13	27.32757732219359	21.272155566628523	26.33224316447808	25.068023946699807
14-15	24.209543246729723	23.03564511065078	23.72125248075489	29.03355916186461
16-17	25.006743311578933	26.173382750897062	24.24328432203769	24.576589615486313
18-19	24.945770391959226	25.48820386911001	25.55583852390158	24.010187215029184
20-21	25.377423393664944	24.668524187448405	25.584488862409156	24.369563556477498
22-23	25.139616965509028	24.30369695087185	25.525145946267948	25.031540137351183
24-25	24.93909427952909	24.54751603492392	25.578328457182426	24.935061228364564
26-27	24.927008809814865	24.733021477662696	25.60839285908949	24.731576853432948
28-29	24.87173028554979	24.887010091230366	25.55013061397698	24.691129009242864
30-31	25.030974267654184	24.81837674811217	25.364916836904772	24.785732147328876
32-33	24.877575929862008	24.75002556501338	25.38798120045505	24.984417304669563
34-35	24.955390895914572	24.790689900973813	25.53602703556287	24.717892167548747
36-37	25.042670025206775	24.75320508162337	25.443874604892404	24.76025028827745
38-39	25.00944727921903	24.854453408429457	25.3986734105706	24.73742590178091
40-41	25.067540913401835	24.76619805389642	25.410973042040258	24.755287990661483
42-43	25.00813681871257	24.717125369391187	25.34714065588829	24.92759715600796
44-45	24.970265148645012	24.746840564035637	25.361486373014568	24.921407914304783
46-47	25.023733092470472	24.80181061936126	25.41245859061725	24.76199769755102
48-49	25.051197599829226	24.843093124559545	25.246985054846625	24.8587242207646
50-51	24.852787793770876	25.027227058157354	25.302049761048778	24.81793538702299
52-53	25.01256727913821	24.97371128123865	25.184269142604528	24.829452297018616
54-55	24.961205318327288	24.756492039203717	25.285306616221177	24.99699602624782
56-57	24.968217016964008	24.924743868633467	25.295754314506162	24.81128479989636
58-59	25.116380497414536	24.830338253083898	25.214897199885588	24.838384049615982
60-61	25.09127682278499	24.843219728447796	25.289461973174426	24.776041475592788
62-63	24.864982755563243	24.996054122637602	25.25907421076471	24.879888911034445
64-65	24.992667102770977	24.84013397890004	25.289935224154487	24.8772636941745
66-67	25.008478739505293	24.918257505124917	25.154048901717356	24.919214853652434
68-69	24.90677495885579	24.939633582896917	25.238136360984527	24.915455097262768
70-71	24.9151996234009	24.792833273721683	25.30301800366319	24.988949099214224
72-73	25.07864379365074	24.81728669028802	25.13204800404755	24.972021512013686
74-75	24.861089969549948	25.08706949415605	25.11881655879373	24.933023977500277
76-77	25.006562138810146	24.82648442779794	25.147173684814494	25.01977974857742
78-79	24.92607392554239	24.81611371163076	25.312717093829583	24.945095268997267
80-81	24.87682617903617	25.01326691852317	25.23282367270319	24.87708322973747
82-83	25.06943528567559	24.869274824045277	25.159327124751652	24.90196276552748
84-85	24.960094014257233	24.992079068400955	25.045387491973827	25.002439425367985
86-87	24.94712837830345	24.955112682934836	25.20741670928659	24.890342229475127
88-89	24.85189942879948	24.838964569889217	25.176838763242195	25.132297238069107
90-91	24.86360045814169	24.927739939909937	25.174706667675768	25.0339529342726
92-93	25.00235595371001	25.041008665565656	25.20018514294367	24.756450237780665
94-95	25.07417629793235	24.882729581424314	25.07478775060033	24.968306370043003
96-97	24.930291768801727	24.848332647165318	25.235974746417643	24.985400837615312
98-99	24.932045356561932	24.897324749775464	25.124205039450604	25.046424854212002
100-101	25.42663405960174	24.32633614218793	25.224962251890197	25.022067546320137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	313.0
1	260.0
2	513.0
3	801.5
4	558.5
5	381.0
6	825.5
7	1872.5
8	2679.5
9	2493.5
10	1684.5
11	1092.5
12	988.0
13	1006.0
14	1026.5
15	1108.5
16	1173.5
17	1271.5
18	1385.0
19	1451.0
20	1504.5
21	1570.0
22	1680.5
23	1843.0
24	2028.5
25	2338.5
26	2766.5
27	3354.5
28	4265.0
29	5663.0
30	7874.5
31	10927.5
32	15774.0
33	22612.0
34	30775.5
35	41014.0
36	53849.5
37	67988.0
38	82350.5
39	97067.5
40	114294.0
41	131548.5
42	148730.0
43	167200.0
44	184906.5
45	203007.0
46	222086.0
47	241602.5
48	260578.5
49	270673.5
50	270585.0
51	271362.5
52	271921.0
53	270274.5
54	266739.5
55	258609.0
56	244207.0
57	224730.0
58	206064.0
59	185540.5
60	162009.5
61	137888.0
62	112646.5
63	86181.5
64	62527.0
65	46224.0
66	35155.5
67	27231.0
68	20770.5
69	15445.0
70	11430.5
71	8566.0
72	6363.0
73	4793.0
74	3668.5
75	2718.0
76	1888.5
77	1147.0
78	664.5
79	376.0
80	202.0
81	95.0
82	59.5
83	39.5
84	16.5
85	8.0
86	4.5
87	2.5
88	3.0
89	2.5
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3234053382349501
2	0.0
3	0.005582370625459438
4	0.06332003252306848
5	5.316543452818512E-4
6	0.06310737078495574
7	0.07336829964889546
8	0.1043105825442992
9	0.09139138195395023
10-11	0.09586613936007247
12-13	0.09589272207733657
14-15	0.06386054777410502
16-17	0.03525754399794143
18-19	0.03835886101208557
20-21	0.0275219732740905
22-23	0.041327264439909236
24-25	0.03320181386285161
26-27	0.020530718633634155
28-29	0.0235877311190048
30-31	0.0031367606371629224
32-33	0.005086159903196377
34-35	0.007407717210927127
36-37	0.011164741250918877
38-39	0.016445841080718598
40-41	0.020973763921369033
42-43	0.0035886668306524955
44-45	0.014452137285911658
46-47	0.024447238977210458
48-49	0.002932959804804879
50-51	0.007071002792248622
52-53	0.002383583648013633
54-55	0.0042177911392360205
56-57	0.0035532232076337057
58-59	0.0011696395596200726
60-61	0.0055469270024406485
62-63	0.014168588301761336
64-65	0.006787453808098301
66-67	0.038722158148028166
68-69	0.061193415141941085
70-71	0.012228049941482579
72-73	0.03230686238162716
74-75	0.05090590356073726
76-77	0.31354315012997175
78-79	0.07728481999247178
80-81	0.03284737763266371
82-83	0.027291589724468365
84-85	0.018900311974769813
86-87	0.11885132888775785
88-89	0.5320796687580768
90-91	0.13095532614867464
92-93	0.04957676769753263
94-95	0.008205198728849904
96-97	0.005617814248478228
98-99	0.010305233392713217
100-101	0.007593796231775775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5642764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.012746924880574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.46574077369685	33.35241031716865
2	16.050134949600395	15.412221348906893
3	5.839160373594985	8.410623878136214
4	2.7381579402271035	5.258659368979048
5	1.5016536270037644	3.6049257781080377
6	0.9567010043642027	2.7560305923190516
7	0.6245275523209972	2.0989698322042205
8	0.45874592535060127	1.7620521613342857
9	0.3480347855417856	1.5039095471345554
>10	1.8207981233483703	16.00080233249561
>50	0.13668894065935072	4.489042517696914
>100	0.05799195039102493	4.573094369223416
>500	0.001294268187969362	0.40105938494246146
>1k	3.6978571260687116E-4	0.37619857135052753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.3165434528185125E-5
2	0.0	0.0	0.0	0.0	5.3165434528185125E-5
3	0.0	0.0	0.0	1.772181150939504E-5	5.3165434528185125E-5
4	0.0	0.0	0.0	3.544362301879008E-5	5.3165434528185125E-5
5	0.0	1.772181150939504E-5	0.0	3.544362301879008E-5	5.3165434528185125E-5
6	0.0	1.772181150939504E-5	0.0	3.544362301879008E-5	1.0633086905637025E-4
7	0.0	1.772181150939504E-5	0.0	3.544362301879008E-5	1.0633086905637025E-4
8	0.0	1.772181150939504E-5	0.0	3.544362301879008E-5	1.0633086905637025E-4
9	0.0	1.772181150939504E-5	0.0	1.240526805657653E-4	1.0633086905637025E-4
10-11	8.86090575469752E-5	1.772181150939504E-5	0.0	1.5063539782985785E-4	1.240526805657653E-4
12-13	8.86090575469752E-5	1.772181150939504E-5	0.0	1.5949630358455538E-4	2.481053611315306E-4
14-15	9.746996330167273E-5	1.772181150939504E-5	0.0	1.683572093392529E-4	3.101317014144132E-4
16-17	1.0633086905637025E-4	1.772181150939504E-5	0.0	1.772181150939504E-4	3.544362301879008E-4
18-19	1.0633086905637025E-4	1.772181150939504E-5	0.0	1.772181150939504E-4	3.721580416972959E-4
20-21	1.0633086905637025E-4	1.772181150939504E-5	0.0	2.658271726409256E-4	4.341843819801785E-4
22-23	1.0633086905637025E-4	1.772181150939504E-5	0.0	3.4557532443320327E-4	5.582370625459437E-4
24-25	1.329135863204628E-4	1.772181150939504E-5	0.0	3.544362301879008E-4	6.114024970741289E-4
26-27	1.5063539782985785E-4	1.772181150939504E-5	0.0	4.1646257047078344E-4	6.468461200929191E-4
28-29	1.5949630358455538E-4	1.772181150939504E-5	0.0	7.443160833945917E-4	6.911506488664066E-4
30-31	1.5949630358455538E-4	3.544362301879008E-5	0.0	0.0023038354962213554	7.088724603758016E-4
32-33	1.5949630358455538E-4	3.544362301879008E-5	0.0	0.0056355360599876225	7.354551776398942E-4
34-35	1.683572093392529E-4	6.202634028288265E-5	0.0	0.009525473686299836	7.620378949039868E-4
36-37	1.772181150939504E-4	8.86090575469752E-5	0.0	0.015382532390154895	7.708988006586842E-4
38-39	1.772181150939504E-4	8.86090575469752E-5	0.0	0.02368520108230647	7.797597064133818E-4
40-41	1.772181150939504E-4	8.86090575469752E-5	0.0	0.03293598669021068	7.797597064133818E-4
42-43	1.772181150939504E-4	8.86090575469752E-5	0.0	0.041929806031228666	7.886206121680793E-4
44-45	1.8607902084864791E-4	8.86090575469752E-5	0.0	0.05229706576422477	9.03812386979147E-4
46-47	1.9493992660334544E-4	8.86090575469752E-5	0.0	0.06343522429787954	9.924214445261224E-4
48-49	1.9493992660334544E-4	8.86090575469752E-5	0.0	0.07467085279483601	0.0010455868790543075
50-51	1.9493992660334544E-4	8.86090575469752E-5	0.0	0.08601281216084883	0.0010455868790543075
52-53	1.9493992660334544E-4	8.86090575469752E-5	0.0	0.09739907605563515	0.0011253350308465851
54-55	1.9493992660334544E-4	9.746996330167273E-5	0.0	0.10963598690287242	0.0012050831826388628
56-57	1.9493992660334544E-4	1.0633086905637025E-4	0.0	0.12202353314793957	0.0012139440883935605
58-59	1.9493992660334544E-4	1.0633086905637025E-4	0.0	0.1350845082303637	0.001222804994148258
60-61	1.9493992660334544E-4	1.240526805657653E-4	0.0	0.1475783853444872	0.001258248617167048
62-63	1.9493992660334544E-4	1.329135863204628E-4	0.0	0.16064822133266604	0.001258248617167048
64-65	1.9493992660334544E-4	1.4177449207516032E-4	0.0	0.1740459108337687	0.0012759704286764428
66-67	1.9493992660334544E-4	1.4177449207516032E-4	0.0	0.18893223250166052	0.0013025531459405354
68-69	1.9493992660334544E-4	1.4177449207516032E-4	0.0	0.2040312159076651	0.001311414051695233
70-71	1.9493992660334544E-4	1.6835720933925288E-4	0.0	0.22027325615602567	0.0014000231092422084
72-73	1.9493992660334544E-4	1.9493992660334544E-4	0.0	0.23594819843608558	0.0015063539782985786
74-75	1.9493992660334544E-4	2.126617381127405E-4	0.0	0.2521990995902008	0.0015949630358455535
76-77	2.3038354962213553E-4	2.126617381127405E-4	0.0	0.26822847810044864	0.0016126848473549487
78-79	2.3038354962213553E-4	2.126617381127405E-4	0.0	0.28427557842220585	0.0016392675646190412
80-81	2.3038354962213553E-4	2.126617381127405E-4	0.0	0.3016340927956583	0.0016747111876378312
82-83	2.3038354962213553E-4	2.126617381127405E-4	0.0	0.3189748853576013	0.0017899029624488991
84-85	2.3038354962213553E-4	2.126617381127405E-4	0.0	0.3370068285684108	0.0017899029624488991
86-87	2.3038354962213553E-4	2.126617381127405E-4	0.0	0.3560843586582746	0.0018164856797129917
88-89	2.3038354962213553E-4	2.126617381127405E-4	0.0	0.37574670852794834	0.0018430683969770842
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	7350	0.0	34.993736	1
TACCTGG	7430	0.0	32.73081	2
GTATCAA	11435	0.0	32.48945	1
TCAACGC	13910	0.0	26.05507	4
GTACATG	17705	0.0	25.503296	1
CAACGCA	14240	0.0	25.438396	5
TATCAAC	14535	0.0	25.162355	2
ATCAACG	14820	0.0	24.550919	3
ACCTGGG	9710	0.0	24.312202	3
TACATGG	18165	0.0	23.84707	2
AACGCAG	15385	0.0	23.731396	6
GGTATCA	7585	0.0	23.673893	1
ACATGGG	18685	0.0	22.319712	3
ACGCAGA	17705	0.0	20.43455	7
GAGTACA	13315	0.0	20.103842	1
AGAGTAC	14910	0.0	19.121422	10-11
CGCAGAG	19015	0.0	19.026918	8
CATGGGG	14825	0.0	18.968014	4
AGTACAT	12740	0.0	18.864994	2
TAACGCA	460	0.0	17.5544	4
>>END_MODULE