FastQCFastQC Report
Mon 15 Aug 2016
SRR2921439_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921439_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4190038
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64660.1543184095227776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78500.040.9299161
GGTATCA46400.034.7220341
TCAACGC95050.032.9337924
CAACGCA98700.031.7158765
ATCAACG100550.031.132343
TATCAAC102900.030.7776762
AACGCAG106600.029.5733576
GTACATG145900.026.5651281
GTACCTG40400.025.0666541
TACATGG151200.024.9471662
ACGCAGA127800.024.374667
TCGCGTA1752.115994E-723.5543779
ACATGGG156650.023.377043
CGCAGAG135700.022.8497358
TACCTGG43000.022.170122
CATGGGG112650.021.482064
GAGTACA110750.021.0310941
AGTACAT105650.020.3892612
GTATAGG10250.019.7598631
GCAGAGT153400.019.6158549
Produced by FastQC (version 0.11.5)