##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921439_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4190038
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.795486580312637	31.0	31.0	34.0	28.0	34.0
2	30.90380206575692	33.0	31.0	34.0	27.0	34.0
3	31.04350843596168	33.0	31.0	34.0	28.0	34.0
4	34.54882748080089	37.0	35.0	37.0	32.0	37.0
5	34.5558467488839	37.0	35.0	37.0	32.0	37.0
6	34.6529057731696	37.0	35.0	37.0	32.0	37.0
7	34.55418876869374	37.0	35.0	37.0	32.0	37.0
8	34.53887625840147	37.0	35.0	37.0	32.0	37.0
9	36.051287363026304	39.0	37.0	39.0	32.0	39.0
10-11	35.94540252379572	39.0	37.0	39.0	31.5	39.0
12-13	35.808636699714896	39.0	37.0	39.0	31.0	39.0
14-15	36.91743547433221	40.0	37.0	41.0	31.0	41.0
16-17	36.792698300110885	40.0	37.0	41.0	31.0	41.0
18-19	36.62670589145015	40.0	37.0	41.0	30.0	41.0
20-21	36.519892779015365	40.0	37.0	41.0	30.0	41.0
22-23	36.57238550103842	40.0	37.0	41.0	30.0	41.0
24-25	36.463985410156184	40.0	37.0	41.0	30.0	41.0
26-27	36.230831200098905	40.0	36.5	41.0	29.0	41.0
28-29	36.0269337652785	39.5	36.0	41.0	28.5	41.0
30-31	35.7929685840558	39.0	36.0	40.5	27.5	41.0
32-33	35.54311476411431	39.0	35.0	40.0	27.0	41.0
34-35	35.151710437948296	39.0	35.0	40.0	25.5	41.0
36-37	34.801254427764135	38.0	35.0	40.0	24.0	41.0
38-39	34.59760913862834	38.0	34.0	40.0	24.0	41.0
40-41	34.3576437731591	38.0	34.0	40.0	23.0	41.0
42-43	34.08639682981395	38.0	33.5	40.0	21.5	41.0
44-45	33.790638056265834	38.0	33.0	40.0	19.5	41.0
46-47	33.52602267568933	38.0	33.0	40.0	18.0	41.0
48-49	33.338211849152685	38.0	33.0	40.0	16.5	41.0
50-51	32.54051991891243	37.0	32.0	39.0	15.0	40.5
52-53	32.981967705304825	37.5	32.5	39.0	18.0	40.0
54-55	33.41642152171412	38.0	33.0	40.0	17.5	41.0
56-57	33.27851286790239	37.5	33.0	40.0	15.0	41.0
58-59	32.96392276155968	37.0	33.0	40.0	12.5	41.0
60-61	32.53186868472315	36.5	32.0	39.5	8.0	41.0
62-63	32.064029490901994	36.0	31.5	39.0	7.0	40.5
64-65	31.546588598957815	35.0	31.0	39.0	2.0	40.0
66-67	31.077645119208945	35.0	31.0	38.0	2.0	40.0
68-69	30.575943463997223	35.0	30.0	37.5	2.0	40.0
70-71	30.083362370460602	34.5	29.5	37.0	2.0	39.0
72-73	29.555358089831167	34.0	29.0	36.0	2.0	39.0
74-75	28.981057809022257	34.0	28.5	36.0	2.0	38.5
76-77	28.42927188249844	34.0	27.5	35.0	2.0	37.0
78-79	27.89918671381978	33.0	26.5	35.0	2.0	37.0
80-81	27.362178934892714	33.0	26.0	35.0	2.0	36.0
82-83	26.771268064871965	32.5	25.0	35.0	2.0	36.0
84-85	26.197718254583847	32.0	24.5	34.5	2.0	35.0
86-87	25.616659443184048	32.0	22.5	34.0	2.0	35.0
88-89	25.01058737414792	31.0	19.5	34.0	2.0	35.0
90-91	24.31687099257811	31.0	16.5	34.0	2.0	35.0
92-93	23.550889156613856	31.0	4.5	34.0	2.0	35.0
94-95	22.69169563617323	30.0	2.0	34.0	2.0	35.0
96-97	21.819075268529787	29.5	2.0	34.0	2.0	35.0
98	21.203083599719143	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103655.0
3	18343.0
4	14126.0
5	12348.0
6	11632.0
7	11902.0
8	13387.0
9	15358.0
10	17224.0
11	19170.0
12	21820.0
13	26413.0
14	21465.0
15	20593.0
16	23325.0
17	26719.0
18	30999.0
19	29674.0
20	30238.0
21	32735.0
22	36008.0
23	39987.0
24	44514.0
25	51037.0
26	58245.0
27	67467.0
28	78363.0
29	92067.0
30	111372.0
31	136818.0
32	173222.0
33	228431.0
34	317863.0
35	466091.0
36	666451.0
37	756161.0
38	349257.0
39	15558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38887019205974	16.877327825191497	12.968032739893578	24.76576924285518
2	16.203826194599312	18.52191288716365	38.739886010768515	26.53437490746852
3	20.472252339171508	21.859492092907736	27.579870945555456	30.0883846223653
4	13.965540957486441	14.365520515519655	34.30536955063972	37.36356897635418
5	15.70745163601624	35.59625029854311	32.57291616909482	16.12338189634583
6	35.61531520816526	32.76329760937507	16.285377059955014	15.336010122504662
7	31.77926096219042	30.129924025533995	18.77322417028137	19.31759084199422
8	28.404731587538713	33.46259928890595	18.531510098499943	19.601159025055395
9	27.1164140827649	16.373671568443747	18.727021055116527	37.782893293674825
10-11	27.03869673505846	24.81450335068391	25.666520420863275	22.480279493394352
12-13	26.962913951229666	21.817660598361655	26.455308340691058	24.764117109717617
14-15	24.08272177473256	23.44652896333895	23.983194312361793	28.487554949566707
16-17	24.59170160058701	26.557835629400156	24.517035865809824	24.333426904203005
18-19	24.54643264765558	25.753840169156216	26.069187222253465	23.630539960934744
20-21	24.997339545956343	24.987509899815244	26.101424988154857	23.913725566073555
22-23	24.913417265705785	24.588242236701525	25.945875756643268	24.55246474094943
24-25	24.565773625490188	24.83566117314146	26.08512109949799	24.51344410187037
26-27	24.52653801019418	25.094581319032415	25.984335482252753	24.39454518852065
28-29	24.564934598800058	25.18542513138946	25.942497874226593	24.307142395583885
30-31	24.692096426163264	25.16053852632087	25.74057757450613	24.406787473009743
32-33	24.581111357289927	25.200503096123068	25.696579939567343	24.52180560701966
34-35	24.697249944932747	25.110523993383783	25.838574209925717	24.353651851757753
36-37	24.74110711157177	25.03648358322846	25.789424493999007	24.43298481120076
38-39	24.67049566548145	25.18190665986931	25.69892475700353	24.44867291764571
40-41	24.79214567517373	25.09546153558937	25.742336874379525	24.370055914857375
42-43	24.737943806779242	25.001770366111714	25.71821052593676	24.542075301172282
44-45	24.60851907893845	25.14037799184139	25.708466193057827	24.542636736162336
46-47	24.725646372071722	25.05483352794101	25.72094734961382	24.498572750373445
48-49	24.788297564480157	25.215649735111	25.464497579549484	24.531555120859355
50-51	24.614115987735037	25.44496371760921	25.43217221443119	24.508748080224564
52-53	24.722652809825814	25.298670734333378	25.44676458002586	24.531911875814945
54-55	24.65351904206013	25.205208767436304	25.60477860553248	24.536493584971083
56-57	24.63238409043069	25.33714576203331	25.530011355200315	24.50045879233568
58-59	24.71636208229981	25.247500043792577	25.474525558427334	24.56161231548028
60-61	24.806596792320654	25.21727036946462	25.5735618565076	24.40257098170712
62-63	24.542157863654186	25.400927271263146	25.513796919360665	24.543117945722003
64-65	24.67279356514817	25.181127846731993	25.588767528830413	24.55731105928942
66-67	24.694915602231685	25.26683665928367	25.46380861026173	24.574439128222917
68-69	24.570772700346875	25.294408212131366	25.544256759675914	24.59056232784584
70-71	24.52877373822909	25.134378197231953	25.617916613192055	24.7189314513469
72-73	24.662610412523346	25.242638925265343	25.46172509764079	24.63302556457052
74-75	24.524712207569046	25.413642607559545	25.39930289325318	24.662342291618234
76-77	24.672226156816762	25.21757055348913	25.370584043858752	24.73961924583536
78-79	24.633792847672716	25.2050331982493	25.526853512462665	24.634320441615316
80-81	24.6205069132436	25.369127388022576	25.411213683633598	24.599152015100227
82-83	24.788444157892616	25.22597138191538	25.3863652499621	24.5992192102299
84-85	24.697913148748977	25.323492574893358	25.28295896994531	24.695635306412353
86-87	24.705043828756434	25.285011302621935	25.44709048151987	24.562854387101762
88-89	24.65091474218809	25.10518878576314	25.408657758114423	24.835238713934352
90-91	24.601713992811902	25.14341725443411	25.520867367890958	24.734001384863028
92-93	24.745287559069617	25.218036343267176	25.441126290707288	24.59554980695592
94-95	24.82942892444175	25.122871129085794	25.350255263061968	24.69744468341049
96-97	24.793356125300104	25.043653694637925	25.416312303261613	24.74667787680036
98	24.659631941855015	25.237988473154584	25.26920951066659	24.833170074323814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1062.0
1	777.0
2	702.0
3	978.0
4	832.0
5	741.0
6	1310.5
7	2627.0
8	3792.5
9	3695.5
10	2796.0
11	2216.5
12	2242.5
13	2544.0
14	2923.5
15	3214.5
16	3462.0
17	3575.5
18	3612.5
19	3547.5
20	3315.5
21	3062.0
22	2846.0
23	2769.5
24	2862.0
25	3191.0
26	3748.5
27	4714.0
28	6167.5
29	8330.5
30	11564.5
31	16037.0
32	22024.0
33	28550.0
34	35855.5
35	45088.0
36	55744.0
37	67234.0
38	79263.0
39	90719.0
40	102508.0
41	115753.0
42	129583.0
43	143035.5
44	155282.5
45	167402.5
46	179467.5
47	189928.5
48	195230.5
49	196313.0
50	196755.0
51	196366.0
52	196159.0
53	195753.0
54	191646.0
55	183180.0
56	170793.5
57	157542.0
58	144130.5
59	128275.5
60	111858.0
61	94560.0
62	76688.5
63	58679.5
64	42896.5
65	32261.5
66	24661.0
67	18654.0
68	13967.0
69	10353.0
70	7716.5
71	5795.0
72	4388.0
73	3188.5
74	2319.0
75	1668.0
76	1096.5
77	665.5
78	391.0
79	228.0
80	128.5
81	89.0
82	65.0
83	32.5
84	21.0
85	18.0
86	11.0
87	12.0
88	11.0
89	9.0
90	9.5
91	6.5
92	7.5
93	8.0
94	6.5
95	4.5
96	2.0
97	3.5
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5054608096633013
2	0.05427158417179033
3	0.06343617886043039
4	0.06143142377229037
5	0.07250530902106377
6	0.06997549902888708
7	0.0489733028674203
8	0.02386613200166681
9	0.01443900986100842
10-11	0.01775640220924011
12-13	0.013484364580941747
14-15	0.017708669945236774
16-17	0.016706292401166768
18-19	0.05798276769804952
20-21	0.0888416763762047
22-23	0.10610882287941065
24-25	0.07419980439318212
26-27	0.09129988797237638
28-29	0.09342397372052473
30-31	0.06318558447441287
32-33	0.03756529177062356
34-35	0.42641618047378094
36-37	0.6656741537904907
38-39	0.6128106714067988
40-41	0.6062594181723412
42-43	0.5783479767963918
44-45	0.5613194916132025
46-47	0.5514866452285159
48-49	0.5401382994617233
50-51	0.5539209906926859
52-53	0.6396004045786696
54-55	0.6100064008966029
56-57	0.5647920138194451
58-59	0.5409258818177782
60-61	0.5542909157387117
62-63	0.5662836470695493
64-65	0.5840519823447902
66-67	0.6143023046569029
68-69	0.5659614542875268
70-71	0.5105681619116581
72-73	0.49340841300245963
74-75	0.47258521283100535
76-77	0.4885635882061212
78-79	0.48124861874761043
80-81	0.4781460215873937
82-83	0.4994226782668797
84-85	0.46358768106637693
86-87	0.4664754830385786
88-89	0.4951864398365838
90-91	0.4761412664992537
92-93	0.47128450863691446
94-95	0.48262092133770623
96-97	0.47735843923133875
98	0.47190502806895784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4190038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.27562948095766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91222571374611	36.87344640849042
2	14.65515271935049	15.029043616485296
3	5.539068557950036	8.520576811412056
4	2.4811367106031277	5.088873866579522
5	1.388371800838342	3.559481902079839
6	0.8650773392184063	2.661443107088144
7	0.607145994912	2.1792255153168587
8	0.424898031538271	1.7429531225875718
9	0.3115537195731235	1.4377601779421103
>10	1.670413722764462	15.492752086110158
>50	0.10675370156977355	3.733996671936241
>100	0.03722147438989358	3.036788513158694
>500	7.4705797524098E-4	0.2529384256126338
>1k	1.8676445663139764E-4	0.23591621720746397
>5k	4.669111415784858E-5	0.1548035579930434
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6466	0.1543184095227776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.386613200166681E-5
2	0.0	0.0	0.0	0.0	2.386613200166681E-5
3	0.0	0.0	0.0	0.0	2.386613200166681E-5
4	0.0	0.0	0.0	0.0	2.386613200166681E-5
5	0.0	0.0	0.0	0.0	2.386613200166681E-5
6	0.0	0.0	0.0	0.0	4.773226400333362E-5
7	0.0	0.0	0.0	0.0	4.773226400333362E-5
8	0.0	0.0	0.0	0.0	4.773226400333362E-5
9	0.0	0.0	0.0	0.0	4.773226400333362E-5
10-11	0.0	0.0	0.0	0.0	4.773226400333362E-5
12-13	2.386613200166681E-5	0.0	0.0	0.0	9.546452800666724E-5
14-15	2.386613200166681E-5	1.1933066000833406E-5	0.0	4.773226400333362E-5	1.1933066000833405E-4
16-17	2.386613200166681E-5	2.386613200166681E-5	0.0	7.159839600500043E-5	1.4319679201000085E-4
18-19	2.386613200166681E-5	2.386613200166681E-5	0.0	7.159839600500043E-5	1.4319679201000085E-4
20-21	2.386613200166681E-5	2.386613200166681E-5	0.0	8.353146200583384E-5	1.5512985801083425E-4
22-23	2.386613200166681E-5	2.386613200166681E-5	0.0	1.3126372600916745E-4	2.505943860175015E-4
24-25	2.386613200166681E-5	2.386613200166681E-5	0.0	3.3412584802333537E-4	2.625274520183349E-4
26-27	2.386613200166681E-5	2.386613200166681E-5	0.0	6.085863660425038E-4	2.863935840200017E-4
28-29	2.386613200166681E-5	2.386613200166681E-5	0.0	0.001264904996088341	2.9832665002083513E-4
30-31	4.773226400333362E-5	2.386613200166681E-5	0.0	0.004689694938327528	3.22192782022502E-4
32-33	7.159839600500043E-5	2.386613200166681E-5	0.0	0.011944999066834237	4.057242440283358E-4
34-35	7.159839600500043E-5	2.386613200166681E-5	0.0	0.020978330029465128	4.295903760300026E-4
36-37	7.159839600500043E-5	3.579919800250022E-5	0.0	0.034426895412404374	4.295903760300026E-4
38-39	7.159839600500043E-5	4.773226400333362E-5	0.0	0.055094965725847836	4.295903760300026E-4
40-41	7.159839600500043E-5	4.773226400333362E-5	0.0	0.0778393895234363	4.295903760300026E-4
42-43	7.159839600500043E-5	4.773226400333362E-5	0.0	0.09924730992893144	4.295903760300026E-4
44-45	7.159839600500043E-5	4.773226400333362E-5	0.0	0.12258838702656158	4.4152344203083596E-4
46-47	7.159839600500043E-5	4.773226400333362E-5	0.0	0.1461800585102092	4.534565080316694E-4
48-49	7.159839600500043E-5	4.773226400333362E-5	0.0	0.16936600574982852	4.773226400333362E-4
50-51	7.159839600500043E-5	4.773226400333362E-5	0.0	0.19298154336547785	4.773226400333362E-4
52-53	7.159839600500043E-5	4.773226400333362E-5	0.0	0.21699087215915464	5.250549040366698E-4
54-55	7.159839600500043E-5	5.9665330004167024E-5	0.0	0.24227703901492065	5.727871680400035E-4
56-57	7.159839600500043E-5	7.159839600500043E-5	0.0	0.26867298100876413	6.682516960466707E-4
58-59	7.159839600500043E-5	7.159839600500043E-5	0.0	0.29407847852453844	6.801847620475041E-4
60-61	7.159839600500043E-5	7.159839600500043E-5	0.0	0.31889925580627193	6.921178280483375E-4
62-63	7.159839600500043E-5	7.159839600500043E-5	0.0	0.34447181624605794	7.159839600500043E-4
64-65	7.159839600500043E-5	7.159839600500043E-5	0.0	0.37077229371189474	7.63716224053338E-4
66-67	7.159839600500043E-5	7.159839600500043E-5	0.0	0.39939971904789406	7.875823560550047E-4
68-69	9.546452800666724E-5	7.159839600500043E-5	0.0	0.42768108546986927	8.114484880566716E-4
70-71	9.546452800666724E-5	7.159839600500043E-5	0.0	0.4572154238219319	8.711138180608387E-4
72-73	9.546452800666724E-5	7.159839600500043E-5	0.0	0.4872748170780313	0.0010859090060758398
74-75	9.546452800666724E-5	7.159839600500043E-5	0.0	0.5175132063241432	0.0011694404680816738
76-77	9.546452800666724E-5	7.159839600500043E-5	0.0	0.5483124496722942	0.0011694404680816738
78-79	1.1933066000833405E-4	7.159839600500043E-5	0.0	0.5819517627286435	0.0011694404680816738
80-81	1.1933066000833405E-4	7.159839600500043E-5	0.0	0.6159729338970195	0.0011694404680816738
82-83	1.1933066000833405E-4	7.159839600500043E-5	0.0	0.6508055535534523	0.0011694404680816738
84-85	1.1933066000833405E-4	7.159839600500043E-5	0.0	0.6864854208959441	0.0011694404680816738
86	1.4319679201000085E-4	7.159839600500043E-5	0.0	0.7131677564738076	0.0011694404680816738
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7850	0.0	40.929916	1
GGTATCA	4640	0.0	34.722034	1
TCAACGC	9505	0.0	32.933792	4
CAACGCA	9870	0.0	31.715876	5
ATCAACG	10055	0.0	31.13234	3
TATCAAC	10290	0.0	30.777676	2
AACGCAG	10660	0.0	29.573357	6
GTACATG	14590	0.0	26.565128	1
GTACCTG	4040	0.0	25.066654	1
TACATGG	15120	0.0	24.947166	2
ACGCAGA	12780	0.0	24.37466	7
TCGCGTA	175	2.115994E-7	23.554377	9
ACATGGG	15665	0.0	23.37704	3
CGCAGAG	13570	0.0	22.849735	8
TACCTGG	4300	0.0	22.17012	2
CATGGGG	11265	0.0	21.48206	4
GAGTACA	11075	0.0	21.031094	1
AGTACAT	10565	0.0	20.389261	2
GTATAGG	1025	0.0	19.759863	1
GCAGAGT	15340	0.0	19.615854	9
>>END_MODULE