FastQCFastQC Report
Mon 15 Aug 2016
SRR2921439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4190038
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.14176482408990085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77800.040.416051
GGTATCA47100.037.223431
TCAACGC98750.031.1223454
CAACGCA102850.029.866695
TATCAAC104950.029.7349552
ATCAACG104550.029.3951023
AACGCAG111300.027.685166
GTACATG144100.025.5236511
TACATGG148800.024.1325532
ACGCAGA130400.023.4127087
ACATGGG148550.022.8627343
GTACCTG43000.022.1590421
CGCAGAG141550.021.5013858
GAGTACA110650.021.0976521
TACCTGG44450.020.410142
CATGGGG114050.020.3249594
AGTACAT106950.020.1187862
GCAGAGT160250.018.9914239
AGAGTAC130650.018.36967510-11
GTGTAGC21150.017.7953361
Produced by FastQC (version 0.11.5)