FastQCFastQC Report
Mon 15 Aug 2016
SRR2921438_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921438_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387398
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76330.1739755545314102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49500.11282313571734318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84000.046.418481
GGTATCA43600.041.7058871
TCAACGC107000.035.734844
CAACGCA108750.034.9911655
TATCAAC110500.034.89342
ATCAACG110350.034.7330973
AACGCAG117850.032.591936
GTACCTG43800.030.0592881
ACGCAGA137650.027.661577
CGCAGAG143000.026.3658378
TACCTGG48150.026.1846032
GTACATG133900.025.578741
TACATGG136950.024.5386982
ACATGGG138400.023.4203243
GCAGAGT163800.022.5428499
GTGTAGC22550.022.4560261
GAGTACA99350.020.712211
AGAGTAC139750.020.02982110-11
CATGGGG112550.019.9192494
AGTACAT96850.019.455922
Produced by FastQC (version 0.11.5)