##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921438_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4387398
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.8373808348365	31.0	31.0	34.0	26.0	34.0
2	29.92787957691552	31.0	31.0	34.0	26.0	34.0
3	30.054897914435845	31.0	31.0	34.0	26.0	34.0
4	33.53554247870834	37.0	35.0	37.0	28.0	37.0
5	33.50605119480841	37.0	35.0	37.0	28.0	37.0
6	33.59918293257188	37.0	35.0	37.0	29.0	37.0
7	33.470557036311725	37.0	35.0	37.0	28.0	37.0
8	33.44271866833144	37.0	35.0	37.0	28.0	37.0
9	34.834085259645924	39.0	35.0	39.0	28.0	39.0
10-11	34.68631601236086	39.0	35.0	39.0	27.0	39.0
12-13	34.4983628109417	38.0	35.0	39.0	27.0	39.0
14-15	35.43490150654215	39.0	36.0	41.0	27.0	41.0
16-17	35.274930949961686	39.0	36.0	41.0	26.0	41.0
18-19	35.088974718044724	39.0	35.0	40.0	25.0	41.0
20-21	34.96253348795801	39.0	35.0	40.0	25.0	41.0
22-23	34.992848836599734	39.0	35.0	40.0	24.5	41.0
24-25	34.84307737752536	39.0	35.0	40.5	23.5	41.0
26-27	34.543729905515754	39.0	34.0	40.0	21.0	41.0
28-29	34.28097781418508	38.5	34.0	40.0	18.5	41.0
30-31	33.993005307473815	38.0	34.0	40.0	17.5	41.0
32-33	33.678445288072794	38.0	33.0	40.0	16.0	41.0
34-35	33.2332105498521	38.0	33.0	40.0	12.0	41.0
36-37	32.82323361135689	38.0	32.0	40.0	9.0	41.0
38-39	32.55684257502966	38.0	31.5	40.0	8.0	41.0
40-41	32.265043198725074	38.0	31.0	40.0	7.5	41.0
42-43	31.943415550629325	37.0	30.5	40.0	2.0	41.0
44-45	31.59105898302365	37.0	30.0	40.0	2.0	41.0
46-47	31.251669668445853	37.0	30.0	40.0	2.0	41.0
48-49	30.987062377290595	36.5	29.5	39.0	2.0	40.0
50-51	30.27948376235755	35.5	28.5	38.5	2.0	39.5
52-53	30.830365287124625	36.5	30.0	39.0	2.0	40.0
54-55	31.24140321894663	37.0	30.0	39.5	2.0	40.5
56-57	31.082034271793898	36.5	30.0	40.0	2.0	41.0
58-59	30.738674836429247	36.0	29.5	39.0	2.0	41.0
60-61	30.27517824915816	35.5	29.0	39.0	2.0	40.0
62-63	29.777627194979804	35.0	28.0	39.0	2.0	40.0
64-65	29.22401193144547	35.0	27.5	38.0	2.0	40.0
66-67	28.728765660193126	34.0	26.0	38.0	2.0	40.0
68-69	28.204409538409784	34.0	26.0	37.0	2.0	39.5
70-71	27.669503085883704	34.0	25.5	36.5	2.0	39.0
72-73	27.097110861608634	33.5	24.0	36.0	2.0	39.0
74-75	26.48380646114166	33.0	22.0	35.5	2.0	38.0
76-77	25.87532781388878	32.0	20.0	35.0	2.0	37.0
78-79	25.281795269086597	32.0	18.0	35.0	2.0	36.5
80-81	24.673061915057627	31.5	11.0	35.0	2.0	36.0
82-83	24.014487060439922	31.0	7.0	34.0	2.0	35.5
84-85	23.362998752335667	30.5	2.0	34.0	2.0	35.0
86-87	22.71300381228236	30.0	2.0	34.0	2.0	35.0
88-89	22.030383726299732	29.5	2.0	34.0	2.0	35.0
90-91	21.26171138337575	29.0	2.0	34.0	2.0	35.0
92-93	20.425288519527975	28.5	2.0	34.0	2.0	35.0
94-95	19.513437463389465	26.5	2.0	33.0	2.0	34.5
96-97	18.6240484679074	25.0	2.0	33.0	2.0	34.0
98	18.01442198770205	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	187893.0
3	30582.0
4	22869.0
5	19000.0
6	18067.0
7	19069.0
8	21472.0
9	24741.0
10	27431.0
11	29694.0
12	32533.0
13	36204.0
14	32603.0
15	33296.0
16	37392.0
17	43045.0
18	48054.0
19	44831.0
20	45340.0
21	49077.0
22	52823.0
23	57616.0
24	63489.0
25	70315.0
26	79136.0
27	89012.0
28	100568.0
29	116015.0
30	136029.0
31	161558.0
32	196844.0
33	248083.0
34	326178.0
35	439475.0
36	572898.0
37	591161.0
38	268495.0
39	14508.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.94655867107718	16.904905028687757	12.52098483000885	24.627551470226216
2	16.095879711840023	18.65557877252205	38.99867349356461	26.249868022073326
3	20.637262045588635	22.37197367919922	27.628927155749345	29.361837119462802
4	13.557380884942674	14.428859360696855	35.1427902909991	36.87096946336137
5	14.984827214992832	36.00266393279043	33.10134918616625	15.911159666050482
6	34.925296404734816	33.518031064707834	16.583999720832278	14.97267280972507
7	31.04188025144934	30.437541728841882	19.17647788508196	19.34410013462682
8	27.94019652565906	34.11919887855947	18.589623027556588	19.350981568224878
9	26.749775693162835	16.573950134584102	18.858164221782932	37.81810995047013
10-11	26.718052673601594	25.21678721007037	26.01493252665851	22.050227589669532
12-13	26.76932791115407	22.142995541290926	26.78897723231789	24.298699315237112
14-15	23.597748473629345	23.69631886106978	24.464536430808216	28.24139623449266
16-17	23.981081191056102	26.96471678149553	24.947139057873315	24.10706296957505
18-19	23.92430946244607	26.03557542382564	26.806041030531958	23.23407408319633
20-21	24.354773989254085	25.284998536597275	26.888970049632505	23.471257424516136
22-23	24.090664492755856	25.02907617118361	26.800729063245903	24.07953027281463
24-25	23.810220030317865	25.25408203157966	26.964742140780533	23.97095579732194
26-27	23.884996466878484	25.449892694622527	26.85093649107138	23.81417434742761
28-29	23.854571726736324	25.585855805677703	26.752668341971635	23.806904125614338
30-31	24.0101605789742	25.490151807743565	26.697860866525065	23.801826746757172
32-33	23.80103722916718	25.550432338421437	26.642473316862432	24.006057115548955
34-35	23.99127609930611	25.58597832731721	26.770583539575572	23.652162033801112
36-37	24.067900746862804	25.49247982738824	26.620542987182894	23.819076438566057
38-39	24.01965840765981	25.587620429523373	26.586987766217156	23.805733396599663
40-41	24.177826412243416	25.470717243250974	26.636702833838832	23.714753510666778
42-43	24.008476861013232	25.41729211358838	26.622572873517704	23.951658151880682
44-45	23.998667444601633	25.622410025875542	26.55754727323904	23.821375256283787
46-47	24.126507863070128	25.591630319724416	26.42312067589562	23.85874114130983
48-49	24.11972401653775	25.655411471990064	26.23031784096166	23.994546670510523
50-51	23.910359706016347	25.965115702309877	26.160748927700133	23.963775663973646
52-53	24.026254317201452	25.938290889809256	26.15551383654108	23.879940956448216
54-55	23.97282160334798	25.728485603124255	26.282547497323215	24.016145296204545
56-57	23.926818166872938	25.966529546389534	26.22577382337364	23.88087846336389
58-59	24.00798695916906	25.874943819143688	26.261452075267	23.855617146420254
60-61	24.06292863739387	25.716536814904707	26.263302579310306	23.957231968391117
62-63	23.943067682024974	25.839646862444305	26.334845983495654	23.882439472035067
64-65	23.947571030411492	25.741420437494007	26.336005177536066	23.975003354558435
66-67	23.938124322609397	25.89621871624509	26.24232978354677	23.92332717759875
68-69	23.951510155094592	25.798549218191585	26.256408047362985	23.993532579350834
70-71	23.875921929460052	25.641292821319706	26.323701827249845	24.159083421970394
72-73	24.001035868669987	25.711489967941652	26.164556576541052	24.122917586847308
74-75	23.837599813193656	25.915431211310942	26.1618965422512	24.085072433244203
76-77	24.0352333098611	25.747660914080058	26.124884274934026	24.092221501124826
78-79	23.955892901337805	25.7107717048058	26.185962663780103	24.147372730076288
80-81	23.93513114535853	25.820009773529534	26.078198996524776	24.166660084587164
82-83	24.10259146339718	25.793924258690886	26.029115278978203	24.07436899893373
84-85	24.09909757818576	25.794281705084497	25.998641417092394	24.10797929963735
86-87	24.003969836385604	25.759218046159248	26.14018911090041	24.09662300655474
88-89	24.03563314257779	25.593821787347164	26.099765700982246	24.270779369092793
90-91	24.015456858855202	25.646948045325253	26.085521191081973	24.25207390473757
92-93	24.060819427273348	25.74329367673414	26.160901852349344	24.034985043643168
94-95	24.108708926384534	25.582435480890904	26.112996272440398	24.195859320284168
96-97	24.120357280628753	25.602192768077803	26.075854024558176	24.201595926735266
98	24.132056323995982	25.620280264738987	25.935854524043737	24.311808887221293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	776.0
1	597.0
2	637.0
3	900.0
4	776.0
5	678.5
6	1075.0
7	1968.0
8	2817.5
9	2941.5
10	2474.5
11	2160.0
12	2330.5
13	2771.0
14	3142.5
15	3501.5
16	4026.5
17	4299.0
18	4526.0
19	4803.5
20	4916.5
21	5191.0
22	5790.0
23	6556.0
24	7806.5
25	9723.5
26	12142.5
27	15288.0
28	19107.0
29	23710.0
30	28992.5
31	34950.5
32	42039.5
33	49262.0
34	57184.5
35	66075.0
36	74962.5
37	84207.5
38	93671.5
39	103548.5
40	113959.5
41	124842.5
42	135483.5
43	146953.0
44	157837.0
45	166731.5
46	175939.0
47	184800.5
48	188896.0
49	188967.5
50	189011.0
51	188116.5
52	187779.5
53	187648.5
54	184471.0
55	177407.5
56	165847.5
57	154316.0
58	142328.5
59	126716.5
60	109300.5
61	90782.0
62	73183.5
63	56271.5
64	41592.5
65	31144.0
66	23469.5
67	17731.0
68	13442.5
69	10275.5
70	7815.0
71	5875.5
72	4379.5
73	3237.0
74	2492.5
75	1865.5
76	1293.5
77	843.5
78	548.0
79	327.0
80	181.5
81	116.5
82	64.5
83	33.0
84	25.5
85	20.0
86	14.5
87	17.5
88	17.0
89	14.0
90	9.5
91	7.5
92	5.0
93	4.0
94	6.0
95	5.0
96	2.5
97	2.5
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4684553350300109
2	0.05002965311102389
3	0.058736408231028964
4	0.05552265830453494
5	0.06616678040150449
6	0.06694172719229029
7	0.044263137285470794
8	0.020444919745142794
9	0.012535903968593685
10-11	0.015168443801998359
12-13	0.010894840176341422
14-15	0.015612898579066682
16-17	0.014872140617286145
18-19	0.05736885507081874
20-21	0.08613305654057371
22-23	0.10287418647681383
24-25	0.07257148770182235
26-27	0.088446500636596
28-29	0.0892898250853923
30-31	0.05945437364013932
32-33	0.033778563057192436
34-35	0.3950975042610677
36-37	0.61750723321659
38-39	0.5672610508551994
40-41	0.5628392956371863
42-43	0.536046649973401
44-45	0.5208440173423975
46-47	0.5106557462988314
48-49	0.501242422046051
50-51	0.5150547089641742
52-53	0.5974042017614996
54-55	0.5672838434078695
56-57	0.5238070491895197
58-59	0.5021655204291928
60-61	0.513949270159671
62-63	0.5263484188122436
64-65	0.5454827667788517
66-67	0.5714092954411704
68-69	0.5256304534031333
70-71	0.4742788322372395
72-73	0.4569678884842451
74-75	0.438483128268737
76-77	0.4518965455151322
78-79	0.44616421851858434
80-81	0.44394194463324277
82-83	0.46290534845482445
84-85	0.43022082792580024
86-87	0.4320556284157489
88-89	0.45848359323681137
90-91	0.4414803489448643
92-93	0.43735489691156354
94-95	0.44812437804821903
96-97	0.4422438994593151
98	0.4369560272398355
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4387398.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.50295883263236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36623691269918	43.33781618561358
2	13.694028918306817	15.748943622845477
3	4.789838192359092	8.262896051705852
4	2.095293986002331	4.819424152774168
5	1.1231804846562192	3.2293100585401318
6	0.6638377507676624	2.290358091236409
7	0.4334302130866999	1.7446463789960502
8	0.3150771929672842	1.4494296685039283
9	0.24141868660079516	1.2494059917330327
>10	1.186158554619276	12.0641071216683
>50	0.06383319205344519	2.5060349074800166
>100	0.026868548730609083	2.595236926252113
>500	4.7842041006219434E-4	0.1825785859803852
>1k	2.790783979343153E-4	0.3448220937013512
>5k	3.9868342561985484E-5	0.1749901629692545
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7633	0.1739755545314102	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4950	0.11282313571734318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.837765801051101E-5
2	0.0	0.0	0.0	2.2792552670170338E-5	6.837765801051101E-5
3	0.0	2.2792552670170338E-5	0.0	2.2792552670170338E-5	6.837765801051101E-5
4	0.0	2.2792552670170338E-5	0.0	4.5585105340340676E-5	6.837765801051101E-5
5	0.0	4.5585105340340676E-5	0.0	4.5585105340340676E-5	6.837765801051101E-5
6	0.0	4.5585105340340676E-5	0.0	4.5585105340340676E-5	1.1396276335085169E-4
7	0.0	4.5585105340340676E-5	0.0	6.837765801051101E-5	1.1396276335085169E-4
8	2.2792552670170338E-5	4.5585105340340676E-5	0.0	6.837765801051101E-5	1.1396276335085169E-4
9	2.2792552670170338E-5	4.5585105340340676E-5	0.0	9.117021068068135E-5	1.1396276335085169E-4
10-11	9.117021068068135E-5	4.5585105340340676E-5	0.0	2.0513297403153304E-4	1.1396276335085169E-4
12-13	9.117021068068135E-5	4.5585105340340676E-5	0.0	2.2792552670170338E-4	2.0513297403153304E-4
14-15	9.117021068068135E-5	4.5585105340340676E-5	0.0	3.0769946104729955E-4	2.3932180303678854E-4
16-17	9.117021068068135E-5	4.5585105340340676E-5	0.0	3.7607711905781054E-4	2.7351063204204406E-4
18-19	9.117021068068135E-5	9.117021068068135E-5	0.0	5.242287114139177E-4	2.7351063204204406E-4
20-21	9.117021068068135E-5	9.117021068068135E-5	0.0	5.242287114139177E-4	2.9630318471221437E-4
22-23	9.117021068068135E-5	9.117021068068135E-5	0.0	6.267951984296842E-4	3.532845663876402E-4
24-25	9.117021068068135E-5	1.1396276335085169E-4	0.0	6.837765801051101E-4	3.646808427227254E-4
26-27	9.117021068068135E-5	1.1396276335085169E-4	0.0	7.179654091103657E-4	4.2166222439815127E-4
28-29	9.117021068068135E-5	1.2535903968593685E-4	0.0	0.001219401567854113	4.330585007332364E-4
30-31	9.117021068068135E-5	1.3675531602102203E-4	0.0	0.0029288430181168886	4.444547770683216E-4
32-33	9.117021068068135E-5	1.5954786869119237E-4	0.0	0.006621236550684483	4.5585105340340676E-4
34-35	9.117021068068135E-5	1.5954786869119237E-4	0.0	0.011612805585451788	4.5585105340340676E-4
36-37	9.117021068068135E-5	1.5954786869119237E-4	0.0	0.01854174159718357	4.5585105340340676E-4
38-39	9.117021068068135E-5	1.823404213613627E-4	0.0	0.029527751984205673	4.900398824086623E-4
40-41	9.117021068068135E-5	1.937366976964479E-4	0.0	0.04108357618798203	5.242287114139177E-4
42-43	9.117021068068135E-5	2.2792552670170338E-4	0.0	0.05151116903458496	5.470212640840881E-4
44-45	9.117021068068135E-5	2.2792552670170338E-4	0.0	0.06359122194977523	5.698138167542584E-4
46-47	9.117021068068135E-5	2.2792552670170338E-4	0.0	0.07527240519323754	5.926063694244287E-4
48-49	9.117021068068135E-5	2.507180793718737E-4	0.0	0.08754619480612427	6.495877510998547E-4
50-51	9.117021068068135E-5	2.7351063204204406E-4	0.0	0.10087983811817391	6.609840274349398E-4
52-53	9.117021068068135E-5	2.7351063204204406E-4	0.0	0.11315362773106064	6.951728564401952E-4
54-55	9.117021068068135E-5	2.7351063204204406E-4	0.0	0.1257123242523245	7.179654091103657E-4
56-57	9.117021068068135E-5	2.7351063204204406E-4	0.0	0.13874966437966194	7.40757961780536E-4
58-59	9.117021068068135E-5	2.7351063204204406E-4	0.0	0.15286965075883246	7.863430671208767E-4
60-61	9.117021068068135E-5	2.7351063204204406E-4	0.0	0.166590767466275	8.433244487963025E-4
62-63	9.117021068068135E-5	2.849069083771292E-4	0.0	0.18017512885769651	8.661170014664728E-4
64-65	9.117021068068135E-5	2.9630318471221437E-4	0.0	0.19453443703990383	8.775132778015579E-4
66-67	9.117021068068135E-5	2.9630318471221437E-4	0.0	0.20928121861750404	8.889095541366432E-4
68-69	9.117021068068135E-5	2.9630318471221437E-4	0.0	0.22426732199814103	8.889095541366432E-4
70-71	9.117021068068135E-5	3.190957373823847E-4	1.1396276335085169E-5	0.23939018069479906	9.230983831418987E-4
72-73	9.117021068068135E-5	3.4188829005255504E-4	2.2792552670170338E-5	0.25483213512883945	0.001071249975498006
74-75	9.117021068068135E-5	3.646808427227254E-4	2.2792552670170338E-5	0.27060458157659734	0.0011738164625137723
76-77	9.117021068068135E-5	4.444547770683216E-4	2.2792552670170338E-5	0.28647959451137095	0.0011852127388488575
78-79	9.117021068068135E-5	4.5585105340340676E-4	2.2792552670170338E-5	0.30377914198803024	0.0011852127388488575
80-81	9.117021068068135E-5	4.672473297384919E-4	2.2792552670170338E-5	0.3220359766768367	0.0011966090151839426
82-83	9.117021068068135E-5	4.7864360607357707E-4	2.2792552670170338E-5	0.33868593640239614	0.001276382949529539
84-85	9.117021068068135E-5	4.900398824086623E-4	2.2792552670170338E-5	0.356464127485129	0.0013105717785347943
86	9.117021068068135E-5	5.014361587437474E-4	2.2792552670170338E-5	0.3705385287589592	0.0013219680548698797
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8400	0.0	46.41848	1
GGTATCA	4360	0.0	41.705887	1
TCAACGC	10700	0.0	35.73484	4
CAACGCA	10875	0.0	34.991165	5
TATCAAC	11050	0.0	34.8934	2
ATCAACG	11035	0.0	34.733097	3
AACGCAG	11785	0.0	32.59193	6
GTACCTG	4380	0.0	30.059288	1
ACGCAGA	13765	0.0	27.66157	7
CGCAGAG	14300	0.0	26.365837	8
TACCTGG	4815	0.0	26.184603	2
GTACATG	13390	0.0	25.57874	1
TACATGG	13695	0.0	24.538698	2
ACATGGG	13840	0.0	23.420324	3
GCAGAGT	16380	0.0	22.542849	9
GTGTAGC	2255	0.0	22.456026	1
GAGTACA	9935	0.0	20.71221	1
AGAGTAC	13975	0.0	20.029821	10-11
CATGGGG	11255	0.0	19.919249	4
AGTACAT	9685	0.0	19.45592	2
>>END_MODULE