FastQCFastQC Report
Mon 15 Aug 2016
SRR2921438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387398
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.18161105967591726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52490.1196381089657241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.047.5155751
GTATCAA81600.044.125611
TCAACGC106100.034.0244144
TATCAAC109900.032.7603952
CAACGCA110300.032.7138985
ATCAACG110150.032.6001663
GTACCTG46250.032.026371
AACGCAG117150.031.1657986
TACCTGG48800.029.7837372
ACGCAGA140550.025.7755377
GTACATG132000.025.6539821
CGCAGAG147900.024.4303978
TACATGG135250.023.84572
ACATGGG134600.022.9377523
GAGTACA102800.022.470241
ACCTGGG65500.021.6826533
AGTACAT98450.021.3247592
GCAGAGT169700.021.2075989
GTGGTAT29250.020.0280341
AGAGTAC146350.019.8382310-11
Produced by FastQC (version 0.11.5)