##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4387398
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84767053273945	33.0	31.0	34.0	30.0	34.0
2	31.965231784305868	34.0	31.0	34.0	30.0	34.0
3	32.08851533414566	34.0	31.0	34.0	30.0	34.0
4	35.639252696017095	37.0	35.0	37.0	35.0	37.0
5	35.43590073205121	37.0	35.0	37.0	33.0	37.0
6	35.47600149336805	37.0	35.0	37.0	33.0	37.0
7	35.40344778385731	37.0	35.0	37.0	33.0	37.0
8	35.35373585893051	37.0	35.0	37.0	33.0	37.0
9	36.95970846501731	39.0	37.0	39.0	33.0	39.0
10-11	36.86516666598289	39.0	37.0	39.0	33.0	39.0
12-13	36.73657712384424	39.0	37.0	39.0	33.0	39.0
14-15	38.00182511365506	40.0	38.0	41.0	33.0	41.0
16-17	37.90402603091856	40.0	38.0	41.0	33.0	41.0
18-19	37.80370529411738	40.0	38.0	41.0	33.0	41.0
20-21	37.68805895886355	40.0	38.0	41.0	32.0	41.0
22-23	37.6497934538877	40.0	38.0	41.0	32.5	41.0
24-25	37.703405526464664	40.0	38.0	41.0	32.0	41.0
26-27	37.58223154133725	40.0	38.0	41.0	32.0	41.0
28-29	37.43528111650687	40.0	37.5	41.0	32.0	41.0
30-31	37.277902187127765	40.0	37.0	41.0	31.5	41.0
32-33	37.09126172277965	40.0	37.0	41.0	31.0	41.0
34-35	36.926674192767564	40.0	37.0	41.0	31.0	41.0
36-37	36.80496036603016	40.0	36.5	41.0	30.5	41.0
38-39	36.74439827433025	40.0	36.0	41.0	30.0	41.0
40-41	36.56138570059065	39.5	36.0	41.0	30.0	41.0
42-43	36.2998151751904	39.0	35.5	41.0	30.0	41.0
44-45	36.060819419619556	39.0	35.0	40.5	29.5	41.0
46-47	35.73736859523572	39.0	35.0	40.0	28.0	41.0
48-49	35.36495036465805	39.0	35.0	40.0	27.0	41.0
50-51	35.06347714522366	38.0	34.5	40.0	26.0	41.0
52-53	34.73453069450275	38.0	34.0	40.0	26.0	41.0
54-55	34.391013876561914	38.0	34.0	40.0	24.0	41.0
56-57	33.99767003130329	37.5	33.0	40.0	23.5	41.0
58-59	33.589955025735065	37.0	33.0	40.0	22.5	41.0
60-61	33.14878966986811	36.5	32.0	39.0	21.5	40.5
62-63	32.692504076448046	36.0	32.0	39.0	19.5	40.0
64-65	32.1576858311008	35.0	31.0	39.0	18.0	40.0
66-67	31.609216100294525	35.0	31.0	38.0	14.5	40.0
68-69	31.089801062041786	35.0	30.0	37.5	10.0	39.5
70-71	30.515832618786806	34.0	29.5	37.0	7.5	39.0
72-73	29.946162964928185	34.0	29.0	36.0	7.0	39.0
74-75	29.398339630915636	34.0	28.5	36.0	4.0	38.5
76-77	28.183863875581835	32.0	27.0	34.5	2.0	36.0
78-79	29.092540954798267	34.0	29.0	35.0	2.0	37.0
80-81	29.096224915086346	34.0	29.0	35.0	2.0	37.0
82-83	28.72146030517405	34.0	29.0	35.0	2.0	36.0
84-85	28.183511502717558	33.5	27.5	35.0	2.0	36.0
86-87	27.617775273635992	33.0	26.5	35.0	2.0	35.0
88-89	27.02905412273972	33.0	25.5	35.0	2.0	35.0
90-91	26.497109220544843	32.0	25.0	34.0	2.0	35.0
92-93	26.004961254939715	32.0	24.0	34.0	2.0	35.0
94-95	25.445034049794433	32.0	21.5	34.0	2.0	35.0
96-97	24.617025170727615	31.5	17.5	34.0	2.0	35.0
98-99	23.537496940099803	31.0	2.0	34.0	2.0	35.0
100-101	20.038388128909208	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30160.0
3	15814.0
4	9381.0
5	7167.0
6	6434.0
7	6889.0
8	7843.0
9	9555.0
10	11313.0
11	13392.0
12	16488.0
13	19426.0
14	22110.0
15	25557.0
16	29819.0
17	36642.0
18	36574.0
19	31054.0
20	30417.0
21	31977.0
22	34350.0
23	36867.0
24	41491.0
25	47692.0
26	56428.0
27	68051.0
28	80975.0
29	98122.0
30	119362.0
31	146425.0
32	185319.0
33	242401.0
34	335088.0
35	486943.0
36	725893.0
37	872023.0
38	396325.0
39	15630.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86929781943928	17.56762596096139	12.988322711554401	24.574753508044925
2	16.255078750548734	19.00734786312981	38.72842172057333	26.009151665748124
3	20.679632917442895	22.52648825554183	27.826487138639287	28.967391688375987
4	13.81772304208937	14.786891683955602	35.058722323924826	36.33666295003021
5	14.994488728104205	35.9766393184804	33.21455303995193	15.814318913463458
6	34.997646335444635	33.61509647743994	16.400412164979112	14.986845022136306
7	31.12895209416256	30.439265145935497	19.112245598532716	19.31953716136923
8	27.98203998451235	34.247328277715376	18.48310014016004	19.287531597612237
9	26.668201247938523	16.616435365421594	18.88420124246331	37.831162144176574
10-11	26.730985921598162	25.26835748632461	26.012875410170423	21.987781181906804
12-13	26.765015048842432	22.098898585574215	26.863025019805608	24.27306134577774
14-15	23.499659377629218	23.714546821720734	24.580357116384747	28.2054366842653
16-17	23.951137302729222	26.98767625162695	25.058953371175214	24.00223307446861
18-19	23.8348727148689	26.094420923793088	26.899723285262432	23.170983076075576
20-21	24.338727054210377	25.277014933607955	27.03013476075597	23.354123251425698
22-23	23.998752730539632	25.032036811550583	26.975189142599156	23.994021315310633
24-25	23.733283355398182	25.27940732370551	27.1174188494108	23.8698904714855
26-27	23.799900079300514	25.43487902653575	27.01750390432781	23.747716989835922
28-29	23.745121349155063	25.608068065094503	26.935145447886338	23.7116651378641
30-31	23.94037829748694	25.487356976555876	26.86864003889442	23.70362468706276
32-33	23.720825116179835	25.47503352963327	26.9001797060698	23.903961648117097
34-35	23.900556158465157	25.533814338945522	27.018590358597866	23.54703914399145
36-37	23.89642806057349	25.57132281832113	26.83576851776396	23.696480603341424
38-39	23.850789149764918	25.5872275242289	26.82144911671237	23.74053420929381
40-41	23.99608067579618	25.503249138458813	26.824254260914326	23.676415924830682
42-43	23.90112651612869	25.477606323606878	26.786389538942796	23.83487762132164
44-45	23.888372754630762	25.59400936245129	26.725990382236077	23.791627500681876
46-47	23.87778076939474	25.65818676246731	26.629522487017056	23.834509981120895
48-49	23.94906138324185	25.748604403114868	26.432228347378288	23.87010586626499
50-51	23.858841973813522	25.976647732796398	26.278030544698943	23.88647974869114
52-53	23.989911307395364	25.972753555271506	26.2401396944992	23.79719544283393
54-55	23.977454543879727	25.776176765930998	26.319823314301942	23.926545375887333
56-57	23.90208183514218	26.017092770275585	26.248331808075015	23.83249358650722
58-59	24.033235525699034	25.907053078809355	26.307043642596838	23.75266775289477
60-61	24.04873135878848	25.799262369264635	26.33044949750459	23.821556774442296
62-63	23.880695173183508	25.89921087599216	26.43652050498283	23.7835734458415
64-65	23.92509907451434	25.80856337509363	26.357422280129345	23.908915270262693
66-67	23.890636112136544	25.980091155574414	26.275153117332305	23.854119614956737
68-69	23.98201763183423	25.877968349088924	26.288289190270024	23.85172482880682
70-71	23.921551821509205	25.731315752311357	26.35319415059095	23.993938275588487
72-73	24.052378542654353	25.77512352101673	26.187663938019075	23.98483399830985
74-75	23.81430937825526	26.00595000282769	26.145783734773815	24.033956884143237
76-77	23.92837813509969	25.86740353594381	26.209293354174466	23.994924974782037
78-79	23.910264758899636	25.82980498616298	26.256730622069487	24.003199632867904
80-81	23.86489425226923	25.998921102053668	26.133110744131784	24.003073901545317
82-83	23.99591173990896	25.949040836547567	26.134565131053677	23.920482292489794
84-85	24.012920777881984	25.96716620563911	26.083680718774403	23.9362322977045
86-87	23.919747680888843	25.982339807246476	26.214265184939578	23.883647326925104
88-89	23.91030368213109	25.851388347420173	26.168922276075683	24.06938569437305
90-91	23.920238902163767	25.891478441065246	26.16387209559738	24.024410561173607
92-93	23.969319151085497	25.996071229572433	26.240054508867782	23.794555110474292
94-95	23.919441314625693	25.898304837482815	26.16797579583193	24.014278052059566
96-97	23.933449977388438	25.897647121037505	26.194856963631857	23.9740459379422
98-99	23.94146642358029	25.899481139150538	26.15076233474161	24.00829010252756
100-101	24.42229998641457	25.275595092102854	26.369917657689037	23.932187263793544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	368.0
1	294.5
2	470.5
3	748.0
4	557.5
5	379.5
6	763.5
7	1654.0
8	2488.0
9	2542.0
10	1935.5
11	1464.0
12	1409.5
13	1526.0
14	1703.0
15	1888.0
16	2173.5
17	2478.5
18	2792.0
19	3121.5
20	3443.5
21	3959.5
22	4733.0
23	5752.5
24	6766.5
25	8330.5
26	10602.5
27	13306.5
28	16608.0
29	20659.0
30	25911.0
31	31595.5
32	37624.0
33	44368.5
34	51370.0
35	58973.0
36	68139.5
37	77948.0
38	87387.0
39	96470.5
40	106129.0
41	116398.5
42	126317.5
43	137738.0
44	150303.0
45	161978.5
46	169972.0
47	176261.0
48	184310.0
49	188784.0
50	187140.0
51	185093.0
52	184760.5
53	183381.0
54	181620.0
55	175327.0
56	163169.5
57	150777.5
58	139180.5
59	124838.5
60	107048.5
61	89648.5
62	73011.0
63	55664.0
64	40519.0
65	30341.5
66	23045.0
67	17634.0
68	13454.0
69	10156.0
70	7721.0
71	5858.5
72	4357.5
73	3300.5
74	2563.0
75	1811.0
76	1303.0
77	848.5
78	491.0
79	277.0
80	152.0
81	86.5
82	50.0
83	26.5
84	14.5
85	5.5
86	1.5
87	1.0
88	1.0
89	1.5
90	1.5
91	1.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30822368975871345
2	0.0
3	0.006040026457595139
4	0.059465769916474405
5	5.926063694244288E-4
6	0.06258834963228775
7	0.07373390788800105
8	0.1037289072019452
9	0.09137534365471288
10-11	0.09479422655523843
12-13	0.09505634091094539
14-15	0.06329491876506303
16-17	0.034564906124313316
18-19	0.03769888211646174
20-21	0.027749932875932387
22-23	0.04143686075436968
24-25	0.03333410828012412
26-27	0.020957752180221625
28-29	0.024331049975406836
30-31	0.003111183439478251
32-33	0.005333457324819859
34-35	0.007726675355187744
36-37	0.011293709848069402
38-39	0.01701464056828216
40-41	0.02114009260158299
42-43	0.0035328456638764024
44-45	0.014119986379170524
46-47	0.024319653699071752
48-49	0.002678124938745015
50-51	0.006530066340003801
52-53	0.0025071807937187373
54-55	0.003931715335604383
56-57	0.0037151860852377653
58-59	0.0010142685938225801
60-61	0.005549986575186477
62-63	0.014473270945558165
64-65	0.00674659559037042
66-67	0.0389524725133211
68-69	0.06059400127364784
70-71	0.012023071533514854
72-73	0.031989347672584066
74-75	0.04991569034767304
76-77	0.2971123203320054
78-79	0.07168257814768571
80-81	0.03284406839771546
82-83	0.026895212150800995
84-85	0.018689893189539678
86-87	0.11782610102844557
88-89	0.5238298417421898
90-91	0.1296440395879289
92-93	0.0468728845662053
94-95	0.008775132778015579
96-97	0.005971648799584628
98-99	0.010940425281681761
100-101	0.007886223223878937
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4387398.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.93255716044353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.38371778976746	36.55206447281624
2	16.061754110669234	16.682559268986378
3	5.837207637829255	9.094233579268359
4	2.608983027433917	5.419646408113556
5	1.3743931483520722	3.5687875368857966
6	0.8607473111809211	2.682048536316075
7	0.5543082028497412	2.0150649700297842
8	0.3928263387034967	1.6320381031077673
9	0.29471718411763914	1.3774875309318828
>10	1.5084380866557767	13.97169130874013
>50	0.0861097920194279	3.052156700328359
>100	0.03552385652179316	3.086125754197746
>500	9.221998644881415E-4	0.3118693943734063
>1k	2.634855260695966E-4	0.2528022402272491
>5k	8.78285086898647E-5	0.30142419567735945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7968	0.18161105967591726	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5249	0.1196381089657241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3675531602102203E-4
2	0.0	0.0	0.0	1.3675531602102203E-4	1.3675531602102203E-4
3	0.0	0.0	0.0	1.3675531602102203E-4	1.3675531602102203E-4
4	0.0	0.0	0.0	1.5954786869119237E-4	1.3675531602102203E-4
5	0.0	2.2792552670170338E-5	0.0	1.823404213613627E-4	1.3675531602102203E-4
6	0.0	2.2792552670170338E-5	0.0	1.823404213613627E-4	2.2792552670170338E-4
7	0.0	2.2792552670170338E-5	0.0	2.2792552670170338E-4	2.2792552670170338E-4
8	0.0	4.5585105340340676E-5	0.0	2.2792552670170338E-4	2.2792552670170338E-4
9	0.0	4.5585105340340676E-5	0.0	3.4188829005255504E-4	2.2792552670170338E-4
10-11	0.0	4.5585105340340676E-5	0.0	3.646808427227254E-4	2.507180793718737E-4
12-13	0.0	4.5585105340340676E-5	0.0	3.874733953928957E-4	5.584175404191732E-4
14-15	0.0	5.6981381675425845E-5	0.0	3.874733953928957E-4	6.72380303770025E-4
16-17	0.0	6.837765801051101E-5	0.0	4.786436060735771E-4	7.293616854454508E-4
18-19	0.0	7.977393434559618E-5	0.0	6.153989220945991E-4	7.749467907857915E-4
20-21	0.0	1.0256648701576652E-4	0.0	6.381914747647695E-4	8.319281724612174E-4
22-23	0.0	1.1396276335085169E-4	0.0	6.609840274349398E-4	8.433244487963025E-4
24-25	0.0	1.2535903968593685E-4	0.0	7.293616854454508E-4	8.433244487963025E-4
26-27	0.0	1.3675531602102203E-4	0.0	7.977393434559618E-4	8.547207251313877E-4
28-29	0.0	1.3675531602102203E-4	0.0	0.001276382949529539	8.661170014664728E-4
30-31	0.0	1.3675531602102203E-4	0.0	0.002906050465446718	8.661170014664728E-4
32-33	0.0	1.3675531602102203E-4	0.0	0.0065414626163388875	9.117021068068135E-4
34-35	0.0	1.3675531602102203E-4	0.0	0.011476050269430764	9.800797648173245E-4
36-37	0.0	1.7094414502627752E-4	0.0	0.018336608623152038	9.800797648173245E-4
38-39	0.0	2.165292503666182E-4	0.0	0.0291972599704882	0.0010028723174874948
40-41	0.0	2.2792552670170338E-4	0.0	0.040525158647562856	0.0010142685938225801
42-43	0.0	2.2792552670170338E-4	0.0	0.050895770112490364	0.0010484574228278355
44-45	0.0	2.2792552670170338E-4	0.0	0.06274789750097895	0.001071249975498006
46-47	0.0	2.2792552670170338E-4	0.0	0.07418975894140445	0.0010940425281681762
48-49	0.0	2.2792552670170338E-4	0.0	0.08596211239554744	0.0011396276335085167
50-51	0.0	2.507180793718737E-4	0.0	0.09878292327251825	0.001208005291519028
52-53	0.0	2.507180793718737E-4	0.0	0.11070342831901733	0.001208005291519028
54-55	0.0	2.621143557069589E-4	0.0	0.12260114081284625	0.001219401567854113
56-57	0.0	2.7351063204204406E-4	0.0	0.13512564850510483	0.0012535903968593685
58-59	0.0	2.7351063204204406E-4	0.0	0.14889235031788772	0.001276382949529539
60-61	0.0	2.7351063204204406E-4	0.0	0.16224878618260755	0.001276382949529539
62-63	0.0	3.1909573738238473E-4	0.0	0.17521774865193446	0.0012991755021997092
64-65	0.0	3.1909573738238473E-4	0.0	0.18876792121435074	0.0012991755021997092
66-67	0.0	3.4188829005255504E-4	0.0	0.20273975600116514	0.0013333643312049646
68-69	0.0	3.4188829005255504E-4	0.0	0.21698510142002161	0.0013675531602102202
70-71	0.0	3.4188829005255504E-4	0.0	0.2314241835365745	0.0014017419892154758
72-73	1.1396276335085169E-5	3.4188829005255504E-4	0.0	0.24614817256150456	0.0015043084762312421
74-75	2.2792552670170338E-5	3.646808427227254E-4	0.0	0.2611228796658065	0.0015840824105768385
76-77	2.2792552670170338E-5	3.874733953928957E-4	0.0	0.27606339794110313	0.0015954786869119236
78-79	2.2792552670170338E-5	3.874733953928957E-4	0.0	0.2924398470346205	0.0015954786869119236
80-81	2.2792552670170338E-5	4.102659480630661E-4	0.0	0.3099103386563061	0.001606874963247009
82-83	2.2792552670170338E-5	4.102659480630661E-4	0.0	0.3258765218017604	0.0017322340029329456
84-85	2.2792552670170338E-5	4.330585007332364E-4	0.0	0.34308489906773904	0.0017322340029329456
86-87	2.2792552670170338E-5	4.330585007332364E-4	0.0	0.360874486426807	0.0017892153846083714
88-89	2.2792552670170338E-5	4.444547770683216E-4	0.0	0.37853871474618894	0.0018689893189539678
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4250	0.0	47.515575	1
GTATCAA	8160	0.0	44.12561	1
TCAACGC	10610	0.0	34.024414	4
TATCAAC	10990	0.0	32.760395	2
CAACGCA	11030	0.0	32.713898	5
ATCAACG	11015	0.0	32.600166	3
GTACCTG	4625	0.0	32.02637	1
AACGCAG	11715	0.0	31.165798	6
TACCTGG	4880	0.0	29.783737	2
ACGCAGA	14055	0.0	25.775537	7
GTACATG	13200	0.0	25.653982	1
CGCAGAG	14790	0.0	24.430397	8
TACATGG	13525	0.0	23.8457	2
ACATGGG	13460	0.0	22.937752	3
GAGTACA	10280	0.0	22.47024	1
ACCTGGG	6550	0.0	21.682653	3
AGTACAT	9845	0.0	21.324759	2
GCAGAGT	16970	0.0	21.207598	9
GTGGTAT	2925	0.0	20.028034	1
AGAGTAC	14635	0.0	19.83823	10-11
>>END_MODULE