FastQCFastQC Report
Mon 15 Aug 2016
SRR2921437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4132682
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52610.12730231844598736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48630.11767176859966483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG54200.032.9543271
TACCTGG54250.031.6901662
GTACATG123600.025.0282691
TACATGG126800.023.8264372
ACCTGGG69500.023.7471053
GTATCAA133150.021.8847981
ACATGGG131450.021.6931573
GAGTACA96200.020.576591
AGTACAT89100.019.9639742
TATAACG3659.094947E-1218.8404542
CATGGGG108800.018.5402984
GTACACG10200.018.5039161
TCAACGC154050.018.4808884
TATCAAC156000.018.367432
CAACGCA156250.018.30875
ATCAACG158650.018.1184233
AGAGTAC120250.017.69355210-11
AACGCAG164900.017.5104456
GTGTAGC23300.017.3862821
GTATAAC9050.016.7859291
Produced by FastQC (version 0.11.5)