##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921437_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4132682
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.539574542633574	31.0	31.0	34.0	27.0	34.0
2	30.644090689774824	31.0	31.0	34.0	27.0	34.0
3	30.778430810790667	33.0	31.0	34.0	27.0	34.0
4	34.27740919819139	37.0	35.0	37.0	30.0	37.0
5	34.27446752496321	37.0	35.0	37.0	30.0	37.0
6	34.36834554412849	37.0	35.0	37.0	31.0	37.0
7	34.266279863778536	37.0	35.0	37.0	30.0	37.0
8	34.24717749877682	37.0	35.0	37.0	30.0	37.0
9	35.7284303510408	39.0	37.0	39.0	30.0	39.0
10-11	35.60900959715749	39.0	37.0	39.0	30.0	39.0
12-13	35.46070408998321	39.0	36.0	39.0	30.0	39.0
14-15	36.51814330258171	40.0	37.0	41.0	30.0	41.0
16-17	36.38291477544123	40.0	36.5	41.0	29.5	41.0
18-19	36.209485994809185	40.0	36.0	41.0	29.0	41.0
20-21	36.096368532589736	40.0	36.0	41.0	28.5	41.0
22-23	36.14001839483416	40.0	36.5	41.0	28.5	41.0
24-25	36.01605071476586	40.0	36.0	41.0	27.5	41.0
26-27	35.759546705988996	39.0	36.0	41.0	27.0	41.0
28-29	35.53563714798284	39.0	36.0	40.5	27.0	41.0
30-31	35.294121589805364	39.0	35.0	40.0	25.5	41.0
32-33	35.03842105441454	39.0	35.0	40.0	25.0	41.0
34-35	34.624054427608996	38.0	34.5	40.0	23.5	41.0
36-37	34.252865693513314	38.0	34.0	40.0	21.5	41.0
38-39	34.02937825847718	38.0	33.5	40.0	19.5	41.0
40-41	33.774333471580924	38.0	33.0	40.0	18.0	41.0
42-43	33.49471819994861	38.0	33.0	40.0	16.0	41.0
44-45	33.183640067152524	38.0	32.5	40.0	15.0	41.0
46-47	32.9006322044619	38.0	32.0	40.0	11.0	41.0
48-49	32.69329965383255	37.5	32.0	40.0	9.0	41.0
50-51	31.925298631736	36.0	30.5	39.0	8.5	40.0
52-53	32.394485348739636	37.0	31.5	39.0	8.5	40.0
54-55	32.82960980302864	38.0	33.0	40.0	8.0	41.0
56-57	32.67670788606527	37.0	32.0	40.0	7.0	41.0
58-59	32.347429586888126	37.0	32.0	40.0	2.0	41.0
60-61	31.894406102380973	36.0	31.0	39.0	2.0	41.0
62-63	31.42012934941522	35.5	31.0	39.0	2.0	40.0
64-65	30.885251272660227	35.0	30.0	38.5	2.0	40.0
66-67	30.399138138380838	35.0	29.5	38.0	2.0	40.0
68-69	29.88931957503626	34.5	29.0	37.0	2.0	39.5
70-71	29.364557931144958	34.0	29.0	36.5	2.0	39.0
72-73	28.815445514559308	34.0	28.0	36.0	2.0	39.0
74-75	28.22569846893615	34.0	26.5	35.5	2.0	38.0
76-77	27.651795734585917	33.0	26.0	35.0	2.0	37.0
78-79	27.096108531941244	33.0	25.5	35.0	2.0	36.5
80-81	26.525322659715897	32.5	24.5	35.0	2.0	36.0
82-83	25.901089655579597	32.0	22.5	34.5	2.0	35.5
84-85	25.296961150168343	31.5	20.0	34.0	2.0	35.0
86-87	24.690661415516608	31.0	18.0	34.0	2.0	35.0
88-89	24.05299040671409	31.0	11.0	34.0	2.0	35.0
90-91	23.322724927782975	30.5	4.5	34.0	2.0	35.0
92-93	22.50845189637141	30.0	2.0	34.0	2.0	35.0
94-95	21.610636264779146	29.0	2.0	34.0	2.0	35.0
96-97	20.71749943499161	29.0	2.0	33.5	2.0	34.5
98	20.095347040977263	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	119779.0
3	20832.0
4	15960.0
5	13775.0
6	12843.0
7	13414.0
8	15089.0
9	17800.0
10	19589.0
11	21710.0
12	24332.0
13	28539.0
14	23913.0
15	23591.0
16	26025.0
17	30233.0
18	33881.0
19	32608.0
20	34011.0
21	36573.0
22	39935.0
23	44111.0
24	49774.0
25	55911.0
26	63096.0
27	73108.0
28	83885.0
29	98505.0
30	117868.0
31	143818.0
32	178699.0
33	233283.0
34	316396.0
35	450844.0
36	625048.0
37	676290.0
38	303736.0
39	13878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.29806613402385	16.796803754018043	13.13227826152934	24.77285185042876
2	16.10618813427275	18.641729681361998	38.705678077180636	26.546404107184618
3	20.667866474161563	22.101267594277825	27.16607584258713	30.06479008897348
4	13.939247348145608	14.51139563166444	34.361703956420754	37.1876530637692
5	15.755342428535766	35.348908389255165	32.38587672829388	16.50987245391519
6	35.377227250702774	32.69095398962491	16.571667814046496	15.36015094562582
7	31.67350816186019	29.832574619747472	18.973795088261582	19.52012213013076
8	28.242313818438898	33.230099815259116	18.63513445810753	19.892451908194456
9	26.987268115449243	16.72908498337515	18.726511452984667	37.55713544819094
10-11	26.897068472178088	24.73131876762035	25.7701767764835	22.601435983718062
12-13	27.057464454832935	21.772075804843837	26.394175701338074	24.776284038985157
14-15	24.001812224675202	23.377819318682906	24.10310851711715	28.51725993952475
16-17	24.477706325801087	26.535394986992344	24.572515353072568	24.414383334133998
18-19	24.48304745265382	25.83614484610335	26.010131978173888	23.67067572306895
20-21	24.98794183064021	24.960719370463654	25.895776970549488	24.15556182834665
22-23	24.733917183797594	24.73574605653857	25.76339086086857	24.76694589879527
24-25	24.546309250589804	24.973692638237	25.89121447325645	24.588783637916745
26-27	24.596688007988675	25.04796438809938	25.979314551565214	24.376033052346727
28-29	24.53782324700899	25.358483153680517	25.77595812583008	24.327735473480416
30-31	24.745142248917627	25.082644297924794	25.697624895007298	24.47458855815028
32-33	24.531111436822727	25.156207430638457	25.676295721347607	24.63638541119121
34-35	24.696671972469602	25.198165725485023	25.768014063597466	24.337148238447913
36-37	24.737793653509716	25.15110531671253	25.69768950048076	24.413411529296997
38-39	24.66703783507707	25.207443298020397	25.71477712002364	24.410741746878887
40-41	24.778718408296395	25.150054734650745	25.64382287151342	24.42740398553944
42-43	24.71395201604616	25.04271874595795	25.608128407263358	24.63520083073253
44-45	24.741888259999058	25.07102840418371	25.544847139136596	24.642236196680642
46-47	24.921433741461843	25.212050435195156	25.46912881898193	24.397387004361075
48-49	24.812212069149016	25.207048699274758	25.37896641376899	24.60177281780724
50-51	24.570981586733105	25.39984623170352	25.44587939894114	24.583292782622234
52-53	24.76673480596858	25.474593748481844	25.271198601936888	24.487472843612693
54-55	24.637253912706267	25.13460454471926	25.425716731544224	24.802424811030253
56-57	24.732637806153605	25.241441435084162	25.439877283669947	24.586043475092286
58-59	24.890339433844712	25.22178425300787	25.396148871258216	24.491727441889203
60-61	24.866706146723722	25.097065953754804	25.466120953010297	24.57010694651117
62-63	24.60052928174416	25.271615511403184	25.565919132144383	24.561936074708274
64-65	24.654517965552557	25.23395181797295	25.442652659493685	24.668877556980807
66-67	24.672719933859895	25.308023285367476	25.326257582205276	24.692999198567353
68-69	24.65930972693557	25.281189847904965	25.365698579764214	24.693801845395253
70-71	24.69720427408795	25.11482671538442	25.439473929970173	24.748495080557454
72-73	24.782245072361274	25.070044000215436	25.437221062572803	24.710489864850487
74-75	24.57208245607659	25.392478822765916	25.35188018491099	24.6835585362465
76-77	24.73894964219093	25.172497719319743	25.27717073104312	24.811381907446204
78-79	24.720029798005218	25.060047016467436	25.46082240646609	24.75910077906126
80-81	24.719442174983673	25.211250296150954	25.322259040095286	24.74704848877009
82-83	24.812977344263697	25.161672100723287	25.277008007127822	24.74834254788519
84-85	24.790398812589114	25.210919911629677	25.274874997705904	24.723806278075305
86-87	24.730205334196793	25.19281986657215	25.39124298317518	24.685731816055874
88-89	24.716464597451644	25.025231042918367	25.28202172234436	24.976282637285628
90-91	24.744627274887694	25.022375844906193	25.258429916471208	24.974566963734905
92-93	24.84943383621727	25.2320787733191	25.31677603893935	24.601711351524283
94-95	24.883433384192504	25.015891717743642	25.317193094126257	24.783481803937597
96-97	24.758887432340178	25.07562194521864	25.40558209326796	24.75990852917322
98	24.832476958592565	25.20515555745717	25.183276187660812	24.779091296289455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	671.0
1	488.5
2	547.0
3	786.5
4	631.0
5	544.5
6	890.5
7	1789.5
8	2558.5
9	2545.5
10	2074.5
11	1737.0
12	1836.0
13	2131.0
14	2493.0
15	2829.0
16	3131.0
17	3281.5
18	3240.0
19	3195.0
20	3108.0
21	2940.0
22	2846.5
23	2965.0
24	3253.5
25	3773.5
26	4619.0
27	5919.5
28	7714.0
29	10252.0
30	13617.5
31	17887.0
32	23388.0
33	29825.5
34	37459.5
35	46499.5
36	56335.5
37	66634.5
38	77149.5
39	87933.5
40	98812.5
41	110097.0
42	122609.0
43	136525.5
44	148912.5
45	160191.5
46	175881.5
47	191725.0
48	196586.0
49	192725.0
50	190385.0
51	188818.0
52	188341.0
53	189885.0
54	188148.5
55	181192.5
56	169189.0
57	157630.0
58	145732.0
59	131095.0
60	114546.0
61	95212.5
62	76150.5
63	57758.5
64	42435.0
65	32160.0
66	24581.5
67	18755.5
68	14309.0
69	10842.0
70	8171.5
71	6149.0
72	4676.0
73	3423.0
74	2621.0
75	1983.0
76	1329.0
77	869.0
78	538.5
79	306.0
80	191.5
81	120.5
82	75.5
83	44.0
84	29.0
85	21.0
86	14.0
87	16.5
88	14.0
89	8.5
90	6.0
91	5.5
92	8.5
93	7.0
94	6.0
95	6.5
96	4.5
97	2.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.49865922420355596
2	0.05236309011920104
3	0.06230820566402157
4	0.05950131173896273
5	0.07159999245042323
6	0.07218072912457334
7	0.048539907014379526
8	0.022963295990352028
9	0.014881377275096416
10-11	0.01793024481438446
12-13	0.013187561975491945
14-15	0.018002836898653223
16-17	0.016732475423949872
18-19	0.06028772598520767
20-21	0.09046183567959015
22-23	0.10782344250053597
24-25	0.07584662938014587
26-27	0.09285737446045933
28-29	0.0951319264342139
30-31	0.06371165262655099
32-33	0.037663193054776535
34-35	0.4204654507653867
36-37	0.6577931716013958
38-39	0.6033128123576893
40-41	0.5972150772791132
42-43	0.569545394492003
44-45	0.5536961227599897
46-47	0.5434001454745369
48-49	0.5322088658164359
50-51	0.5469087628808604
52-53	0.6326037183601352
54-55	0.6004696223904961
56-57	0.5575435032262342
58-59	0.5333824378454476
60-61	0.5466183945437854
62-63	0.5601810156213326
64-65	0.5790428588504994
66-67	0.605853535307096
68-69	0.5576886873947717
70-71	0.5041278278851361
72-73	0.4860886949443485
74-75	0.4656419245419803
76-77	0.4808983609191319
78-79	0.47559913876751225
80-81	0.4716670675362876
82-83	0.49231951551075065
84-85	0.45630174303273274
86-87	0.4597014723126532
88-89	0.4885084310866406
90-91	0.47014263376664356
92-93	0.46478291821146656
94-95	0.476458145098026
96-97	0.47083225856719685
98	0.46502489182569573
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4132682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.73242217717998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40220098384918	42.02347298320458
2	13.798499576299484	15.380476075959237
3	4.559017158305003	7.622552069389853
4	2.0600338957406046	4.5924271510686445
5	1.0825366688128528	3.0166195324277987
6	0.6605791862267172	2.2089406852947238
7	0.45260258753108296	1.7657246940736455
8	0.3196077714009025	1.4250012197458184
9	0.2536677101237493	1.2723764322001776
>10	1.2947432960880756	13.007883466458187
>50	0.08067175096230683	3.05527142023146
>100	0.033704892845521486	3.237203874341691
>500	0.0013939734876907185	0.5360819453079277
>1k	6.969866604831083E-4	0.7282420408737122
>5k	4.356166628019193E-5	0.1277264094224724
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5261	0.12730231844598736	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4863	0.11767176859966483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.4197361422921E-5	0.0	0.0	0.0
4	0.0	2.4197361422921E-5	0.0	0.0	0.0
5	0.0	2.4197361422921E-5	0.0	0.0	2.4197361422921E-5
6	0.0	2.4197361422921E-5	0.0	0.0	2.4197361422921E-5
7	0.0	2.4197361422921E-5	0.0	2.4197361422921E-5	2.4197361422921E-5
8	0.0	2.4197361422921E-5	0.0	2.4197361422921E-5	2.4197361422921E-5
9	0.0	2.4197361422921E-5	0.0	2.4197361422921E-5	2.4197361422921E-5
10-11	2.4197361422921E-5	3.62960421343815E-5	0.0	3.62960421343815E-5	3.62960421343815E-5
12-13	2.4197361422921E-5	4.8394722845842E-5	0.0	7.2592084268763E-5	1.93578891383368E-4
14-15	2.4197361422921E-5	4.8394722845842E-5	0.0	7.2592084268763E-5	2.540722949406705E-4
16-17	2.4197361422921E-5	4.8394722845842E-5	0.0	9.6789445691684E-5	2.90368337075052E-4
18-19	2.4197361422921E-5	4.8394722845842E-5	0.0	1.93578891383368E-4	3.14565698497973E-4
20-21	2.4197361422921E-5	4.8394722845842E-5	0.0	2.4197361422920998E-4	3.62960421343815E-4
22-23	2.4197361422921E-5	4.8394722845842E-5	0.0	3.14565698497973E-4	3.87157782766736E-4
24-25	2.4197361422921E-5	4.8394722845842E-5	0.0	4.3555250561257803E-4	4.11355144189657E-4
26-27	3.62960421343815E-5	4.8394722845842E-5	0.0	5.80736674150104E-4	4.5974986703549897E-4
28-29	4.8394722845842E-5	4.8394722845842E-5	0.0	0.001016289179762682	4.8394722845841997E-4
30-31	4.8394722845842E-5	4.8394722845842E-5	0.0	0.0026980057986556913	4.960459091698805E-4
32-33	4.8394722845842E-5	4.8394722845842E-5	0.0	0.006255017927825079	5.928353548615645E-4
34-35	4.8394722845842E-5	4.8394722845842E-5	0.0	0.010537950899682095	6.77526119841788E-4
36-37	4.8394722845842E-5	4.8394722845842E-5	0.0	0.01626062687620291	6.896248005532485E-4
38-39	4.8394722845842E-5	4.8394722845842E-5	0.0	0.025794387276833787	7.138221619761695E-4
40-41	4.8394722845842E-5	4.8394722845842E-5	0.0	0.03714294978418373	7.2592084268763E-4
42-43	4.8394722845842E-5	4.8394722845842E-5	0.0	0.047572012557462684	7.380195233990905E-4
44-45	4.8394722845842E-5	4.8394722845842E-5	0.0	0.05922304208259914	7.864142462449325E-4
46-47	4.8394722845842E-5	4.8394722845842E-5	0.0	0.07082567688488976	8.95302372648077E-4
48-49	4.8394722845842E-5	4.8394722845842E-5	0.0	0.08202905522370219	9.436970954939189E-4
50-51	4.8394722845842E-5	4.8394722845842E-5	0.0	0.09417613065800853	9.678944569168399E-4
52-53	4.8394722845842E-5	4.8394722845842E-5	0.0	0.10623851532733464	9.92091818339761E-4
54-55	4.8394722845842E-5	4.8394722845842E-5	0.0	0.11859126833373582	0.001040486541185603
56-57	4.8394722845842E-5	7.2592084268763E-5	0.0	0.1325047511519154	0.001088881264031445
58-59	4.8394722845842E-5	8.46907649802235E-5	0.0	0.1465634181386325	0.001113078625454366
60-61	4.8394722845842E-5	9.6789445691684E-5	0.0	0.16018653261973703	0.001113078625454366
62-63	4.8394722845842E-5	1.088881264031445E-4	0.0	0.17515260065981364	0.001137275986877287
64-65	4.8394722845842E-5	1.2098680711460499E-4	0.0	0.1891023795201276	0.001137275986877287
66-67	4.8394722845842E-5	1.2098680711460499E-4	0.0	0.20357240165103435	0.0011735720290116684
68-69	4.8394722845842E-5	1.2098680711460499E-4	0.0	0.21953056150945077	0.00120986807114605
70-71	4.8394722845842E-5	1.2098680711460499E-4	0.0	0.23665019471616738	0.0012219667518575104
72-73	4.8394722845842E-5	1.2098680711460499E-4	0.0	0.2529229202730817	0.001306657516837734
74-75	4.8394722845842E-5	1.2098680711460499E-4	0.0	0.27056279675039113	0.0013671509203950364
76-77	4.8394722845842E-5	1.2098680711460499E-4	0.0	0.2884204494805068	0.001379249601106497
78-79	7.2592084268763E-5	1.2098680711460499E-4	0.0	0.30840747001583957	0.001403446962529418
80-81	8.46907649802235E-5	1.2098680711460499E-4	0.0	0.327886345961291	0.001476039046798181
82-83	9.6789445691684E-5	1.2098680711460499E-4	0.0	0.34724423509962776	0.001597025853912786
84-85	9.6789445691684E-5	1.2098680711460499E-4	0.0	0.36713446618926887	0.001669617938181549
86	9.6789445691684E-5	1.2098680711460499E-4	0.0	0.3821005342293455	0.001669617938181549
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5420	0.0	32.954327	1
TACCTGG	5425	0.0	31.690166	2
GTACATG	12360	0.0	25.028269	1
TACATGG	12680	0.0	23.826437	2
ACCTGGG	6950	0.0	23.747105	3
GTATCAA	13315	0.0	21.884798	1
ACATGGG	13145	0.0	21.693157	3
GAGTACA	9620	0.0	20.57659	1
AGTACAT	8910	0.0	19.963974	2
TATAACG	365	9.094947E-12	18.840454	2
CATGGGG	10880	0.0	18.540298	4
GTACACG	1020	0.0	18.503916	1
TCAACGC	15405	0.0	18.480888	4
TATCAAC	15600	0.0	18.36743	2
CAACGCA	15625	0.0	18.3087	5
ATCAACG	15865	0.0	18.118423	3
AGAGTAC	12025	0.0	17.693552	10-11
AACGCAG	16490	0.0	17.510445	6
GTGTAGC	2330	0.0	17.386282	1
GTATAAC	905	0.0	16.785929	1
>>END_MODULE