FastQCFastQC Report
Mon 15 Aug 2016
SRR2921437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4132682
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53110.12851218651713342No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52980.12819762081863545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG54400.036.347161
TACCTGG56600.033.8206062
ACCTGGG75250.025.1866073
GTATCAA139050.023.060321
GTACATG118650.022.6883031
TACATGG122200.021.6898122
GAGTACA96000.020.7456151
ACATGGG123200.020.3961523
TCAACGC158450.019.7566014
AGTACAT89950.019.7498232
CAACGCA159750.019.5540055
TATCAAC164200.019.2661022
ATCAACG166200.018.9203993
AACGCAG168050.018.7021376
AGAGTAC125900.018.53446610-11
GTGTAGC22550.017.9594751
CCTGGGG84400.017.335144
GTATATA9800.017.0162281
CATGGGG101550.016.6996524
ACGCAGA190700.016.4565337
Produced by FastQC (version 0.11.5)