##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4132682
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.942027961502966	33.0	31.0	34.0	30.0	34.0
2	32.06134732844192	34.0	31.0	34.0	30.0	34.0
3	32.18767376730172	34.0	31.0	34.0	30.0	34.0
4	35.71948119889215	37.0	35.0	37.0	35.0	37.0
5	35.522611466355265	37.0	35.0	37.0	33.0	37.0
6	35.55644518499125	37.0	35.0	37.0	33.0	37.0
7	35.49262851581612	37.0	35.0	37.0	33.0	37.0
8	35.44540107368532	37.0	35.0	37.0	33.0	37.0
9	37.07149981537413	39.0	37.0	39.0	34.0	39.0
10-11	36.982678560799016	39.0	37.0	39.0	33.0	39.0
12-13	36.864638750332105	39.0	37.0	39.0	33.0	39.0
14-15	38.163509556263946	40.0	38.0	41.0	33.0	41.0
16-17	38.06520112120894	40.0	38.0	41.0	33.0	41.0
18-19	37.96614583459362	40.0	38.0	41.0	33.0	41.0
20-21	37.852994617054975	40.0	38.0	41.0	33.0	41.0
22-23	37.81614578619889	40.0	38.0	41.0	33.0	41.0
24-25	37.87081101328387	40.0	38.0	41.0	33.0	41.0
26-27	37.75793927526966	40.0	38.0	41.0	33.0	41.0
28-29	37.62438532652645	40.0	38.0	41.0	32.0	41.0
30-31	37.48509805496769	40.0	37.5	41.0	32.0	41.0
32-33	37.32474952101323	40.0	37.0	41.0	32.0	41.0
34-35	37.17424653046133	40.0	37.0	41.0	31.0	41.0
36-37	37.06947727891959	40.0	37.0	41.0	31.0	41.0
38-39	37.035491963814295	40.0	37.0	41.0	31.0	41.0
40-41	36.87463298652062	40.0	37.0	41.0	31.0	41.0
42-43	36.637507434639296	40.0	36.0	41.0	30.0	41.0
44-45	36.425208254591084	39.0	36.0	41.0	30.0	41.0
46-47	36.143357146763286	39.0	35.0	40.5	29.5	41.0
48-49	35.79867734802726	39.0	35.0	40.0	28.5	41.0
50-51	35.520995203599014	38.5	35.0	40.0	28.0	41.0
52-53	35.20885698439899	38.0	34.5	40.0	27.0	41.0
54-55	34.878012148043325	38.0	34.0	40.0	26.5	41.0
56-57	34.48894712924924	38.0	34.0	40.0	26.0	41.0
58-59	34.093050469404616	37.0	33.0	40.0	25.0	41.0
60-61	33.655562416851815	36.5	33.0	39.0	24.0	41.0
62-63	33.21577065450475	36.0	32.5	39.0	22.5	40.0
64-65	32.696949462842774	35.5	32.0	39.0	22.0	40.0
66-67	32.155391220519746	35.0	31.0	38.0	20.0	40.0
68-69	31.641063599860814	35.0	31.0	37.5	18.5	39.5
70-71	31.074995971139323	34.5	30.0	37.0	17.0	39.0
72-73	30.518223395848025	34.0	30.0	36.0	13.0	39.0
74-75	29.997333087810773	34.0	29.0	35.5	9.0	38.0
76-77	28.71909077446559	32.5	28.0	34.5	7.5	36.0
78-79	29.651293518349583	34.0	29.5	35.0	7.5	37.0
80-81	29.674665386787563	34.0	30.0	35.0	7.0	36.5
82-83	29.327208456881028	34.0	29.0	35.0	2.0	36.0
84-85	28.832485901407367	34.0	29.0	35.0	2.0	35.5
86-87	28.3133724056194	33.0	28.5	35.0	2.0	35.0
88-89	27.762613358588926	33.0	27.0	35.0	2.0	35.0
90-91	27.275855364627617	33.0	26.0	35.0	2.0	35.0
92-93	26.825868044045006	32.5	25.0	34.0	2.0	35.0
94-95	26.319654887552442	32.0	25.0	34.0	2.0	35.0
96-97	25.530475124870485	32.0	23.5	34.0	2.0	35.0
98-99	24.50166175379572	31.5	10.5	34.0	2.0	35.0
100-101	20.908721987319616	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26764.0
3	14010.0
4	8568.0
5	6281.0
6	5596.0
7	5818.0
8	6798.0
9	7927.0
10	9550.0
11	11180.0
12	13591.0
13	15799.0
14	17541.0
15	20013.0
16	22828.0
17	26804.0
18	27638.0
19	24438.0
20	24129.0
21	25607.0
22	27493.0
23	30312.0
24	34446.0
25	40339.0
26	48396.0
27	57440.0
28	69644.0
29	84912.0
30	103701.0
31	129388.0
32	165789.0
33	221891.0
34	313593.0
35	472662.0
36	732614.0
37	891850.0
38	376312.0
39	11020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.610660189908835	17.237424881868353	13.484570969413825	24.667343958808985
2	16.21571657340197	18.903898243319954	38.348075172490894	26.532310010787185
3	20.65573246843191	22.19609008480993	27.416642608913865	29.731534837844293
4	14.140834498580517	14.806254199429786	34.31001022996229	36.74290107202741
5	15.785532044221354	35.34867581782598	32.42763021624792	16.43816192170474
6	35.44204829300845	32.80744395339805	16.427947416388943	15.32256033720456
7	31.77631652233043	29.88280843244594	18.911155356681885	19.429719688541745
8	28.316851110112555	33.2870256541313	18.54250717930519	19.85361605645096
9	26.897942169740418	16.73977739557228	18.795500438519046	37.56677999616826
10-11	26.894852526468014	24.826859888427343	25.754783099660035	22.523504485444604
12-13	27.057579240745543	21.769703625556964	26.448194815054972	24.72452231864252
14-15	23.94859224706793	23.40823285334749	24.16690557803152	28.476269321553065
16-17	24.43013801690983	26.542015081741177	24.709083902352116	24.318762998996874
18-19	24.447312448491818	25.872796260776852	26.128532225164324	23.551359065567002
20-21	24.92358011749383	24.927247110034706	26.12594219319072	24.023230579280742
22-23	24.63429628201371	24.619493232173912	26.063111344473988	24.68309914133838
24-25	24.45555073316594	24.934988513003386	26.090901587023406	24.518559166807265
26-27	24.504851826456775	24.966836030217756	26.24377895721007	24.2845331861154
28-29	24.43820751617357	25.344573680794674	26.005142964813082	24.212075838218674
30-31	24.60794239690884	25.080373986462618	25.879806589432786	24.43187702719576
32-33	24.37727279051942	25.077656763939533	25.96973101291668	24.57533943262437
34-35	24.627938658141087	25.07926785960384	26.066200354060083	24.22659312819499
36-37	24.576335173104415	25.15453568871141	25.941522432147195	24.327606706036978
38-39	24.574584573706062	25.142216566083587	25.987099921938352	24.296098938272
40-41	24.699010525464875	25.10273394890204	25.841046094076926	24.35720943155616
42-43	24.61088337214931	25.085144500341013	25.789567102117868	24.51440502539181
44-45	24.6596435252135	24.994654656958453	25.765497289938306	24.580204527889745
46-47	24.72921843866837	25.149469437198636	25.805192659917626	24.316119464215376
48-49	24.685973370333702	25.23523186471184	25.60299319685092	24.475801568103538
50-51	24.468855870680475	25.394964185461234	25.629864001548736	24.506315942309552
52-53	24.641065021718912	25.462671802772753	25.459695448816028	24.436567726692303
54-55	24.621695354597538	25.127888192084434	25.536985216754154	24.713431236563878
56-57	24.65412296269266	25.284014327272974	25.5637818696404	24.498080840393964
58-59	24.85536145933237	25.22385565896967	25.46856668375425	24.452216197943706
60-61	24.824354611789452	25.124622683651488	25.60588402976038	24.44513867479868
62-63	24.515776089307167	25.25700156531487	25.722106756990915	24.505115588387046
64-65	24.63016052895157	25.203555995654824	25.580818512046626	24.585464963346972
66-67	24.63101062409573	25.32529621181478	25.40550524184832	24.638187922241176
68-69	24.630297841554867	25.26799880585422	25.487930338590694	24.61377301400022
70-71	24.676295676407968	25.17243982937762	25.543177597415013	24.608086896799403
72-73	24.836687131428842	25.07667780773661	25.477751484632183	24.608883576202363
74-75	24.507845671907123	25.451464010161263	25.371003339838055	24.669686978093562
76-77	24.64356929514868	25.12563094612488	25.5153581298144	24.71544162891205
78-79	24.612384281933515	25.121411798550973	25.60646570647924	24.659738213036267
80-81	24.633839701073676	25.308624194839467	25.41403847052237	24.643497633564486
82-83	24.693146587477337	25.226144871649335	25.418373761022846	24.662334779850482
84-85	24.673479513056947	25.252631961083278	25.428350153682327	24.645538372177448
86-87	24.6247662340667	25.271831064223537	25.507889384485928	24.595513317223837
88-89	24.59429559814601	25.137793228356703	25.40860543996341	24.85930573353387
90-91	24.613279617811166	25.124295083718902	25.37462372865504	24.887801569814894
92-93	24.731824465161996	25.398516954794477	25.45551711699498	24.414141463048548
94-95	24.756995742116743	25.175572648393707	25.396634106382955	24.6707975031066
96-97	24.503415425507715	25.213893623796956	25.57123802277983	24.711452927915502
98-99	24.63110455130269	25.26300237801122	25.438355446671775	24.66753762401432
100-101	25.040482447442393	24.55673792117868	25.601180830310334	24.801598801068593
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	254.0
1	224.0
2	422.0
3	633.0
4	462.0
5	361.0
6	716.0
7	1606.5
8	2332.5
9	2214.0
10	1625.5
11	1181.0
12	1080.0
13	1099.5
14	1168.0
15	1280.5
16	1381.5
17	1506.0
18	1639.5
19	1729.0
20	1830.5
21	2001.0
22	2220.5
23	2519.0
24	2896.5
25	3457.5
26	4205.5
27	5302.5
28	6837.0
29	8873.5
30	11733.5
31	15274.0
32	20017.5
33	25741.0
34	32023.5
35	39518.0
36	48744.5
37	58676.5
38	68793.5
39	79119.5
40	89523.0
41	99893.5
42	111307.0
43	124753.5
44	138739.5
45	152703.0
46	162810.5
47	174465.0
48	192881.0
49	198453.5
50	190136.5
51	185473.0
52	186152.5
53	186839.5
54	184917.5
55	179020.0
56	167642.5
57	154362.0
58	142795.5
59	130192.5
60	112616.0
61	93896.0
62	76869.5
63	58768.5
64	42817.5
65	32152.5
66	24569.0
67	19113.5
68	14683.0
69	10992.0
70	8229.0
71	6221.5
72	4573.5
73	3377.5
74	2616.0
75	1996.0
76	1416.5
77	897.5
78	534.5
79	317.0
80	191.5
81	106.0
82	45.5
83	20.0
84	12.0
85	6.0
86	2.5
87	1.5
88	0.5
89	1.0
90	1.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3227202092974974
2	0.0
3	0.006412300777074064
4	0.0642923893007011
5	4.35552505612578E-4
6	0.06596200723888265
7	0.07752834599903889
8	0.10881553431887572
9	0.09710401139018196
10-11	0.10069731956148574
12-13	0.10093929317571496
14-15	0.06867211171824979
16-17	0.03857059410813607
18-19	0.04025231072702908
20-21	0.029786951911615747
22-23	0.04298661256781915
24-25	0.03602987115872937
26-27	0.022721322376122816
28-29	0.02464501260924504
30-31	0.003048867539288046
32-33	0.005674281253674974
34-35	0.008045622673121233
36-37	0.011251773061658265
38-39	0.016732475423949872
40-41	0.02160824375066845
42-43	0.003593308171303769
44-45	0.01404656830600564
46-47	0.023822302320865723
48-49	0.0026738084372327706
50-51	0.006823655921263722
52-53	0.002637512395098389
54-55	0.004016761996204886
56-57	0.003484420044900624
58-59	0.0012703614747033524
60-61	0.005033051175967568
62-63	0.014663601022290126
64-65	0.006799458559840801
66-67	0.03873997563809652
68-69	0.061291916484258885
70-71	0.012485838494227236
72-73	0.03076694504924405
74-75	0.050632978777462194
76-77	0.31158942304295373
78-79	0.07600391222939486
80-81	0.032981003619441326
82-83	0.02780276827493623
84-85	0.01892233663272422
86-87	0.11828880131594931
88-89	0.5343866283444988
90-91	0.1332669680367374
92-93	0.04820114395445863
94-95	0.008396484413753586
96-97	0.006146129801421934
98-99	0.010828319236757148
100-101	0.008118214757389995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4132682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.12518117913982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34674767368486	37.71087330212336
2	15.135739236027357	15.779062999162827
3	5.283602109123391	8.262261516496263
4	2.3239514562782695	4.845455628401226
5	1.2772235387429296	3.328775418161866
6	0.8010064750143338	2.5051564581471766
7	0.5212131181682091	1.901782975222362
8	0.37373264329614964	1.558470539549651
9	0.28846024141786375	1.3532438112196103
>10	1.5064250743955558	14.034755004470059
>50	0.09847241295432416	3.5073946047624776
>100	0.04082243342329467	3.656363928755715
>500	0.0017667201653184873	0.6288576623834912
>1k	7.438820517397468E-4	0.6704437548046596
>5k	9.298525646745981E-5	0.2571023963391376
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5311	0.12851218651713342	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5298	0.12819762081863545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4197361422921E-5
2	0.0	0.0	0.0	2.4197361422921E-5	2.4197361422921E-5
3	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
4	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
5	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
6	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
7	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
8	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
9	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
10-11	0.0	0.0	0.0	4.8394722845842E-5	2.4197361422921E-5
12-13	0.0	0.0	0.0	6.04934035573025E-5	6.04934035573025E-5
14-15	0.0	1.20986807114605E-5	0.0	7.2592084268763E-5	1.814802106719075E-4
16-17	0.0	2.4197361422921E-5	0.0	7.2592084268763E-5	2.66170975652131E-4
18-19	0.0	2.4197361422921E-5	0.0	1.2098680711460499E-4	2.66170975652131E-4
20-21	0.0	2.4197361422921E-5	0.0	1.45184168537526E-4	2.66170975652131E-4
22-23	1.20986807114605E-5	2.4197361422921E-5	0.0	2.4197361422921E-4	3.024670177865125E-4
24-25	2.4197361422921E-5	2.4197361422921E-5	0.0	3.14565698497973E-4	3.38763059920894E-4
26-27	2.4197361422921E-5	2.4197361422921E-5	0.0	4.11355144189657E-4	3.750591020552755E-4
28-29	2.4197361422921E-5	2.4197361422921E-5	0.0	8.227102883793141E-4	3.87157782766736E-4
30-31	2.4197361422921E-5	2.4197361422921E-5	0.0	0.0024802295458494026	4.11355144189657E-4
32-33	2.4197361422921E-5	3.62960421343815E-5	0.0	0.0059767482714614874	5.32341951304262E-4
34-35	2.4197361422921E-5	4.8394722845842E-5	0.0	0.01015079311691536	5.56539312727183E-4
36-37	2.4197361422921E-5	8.46907649802235E-5	0.0	0.01572828492489865	5.56539312727183E-4
38-39	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.0251773545605493	5.80736674150104E-4
40-41	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.03615085796584397	5.80736674150104E-4
42-43	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.046374243167028095	6.170327162844855E-4
44-45	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.05788008852362703	6.29131396995946E-4
46-47	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.06932544047666866	6.53328758418867E-4
48-49	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.08032314124338626	7.98512926956393E-4
50-51	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.09221614438275193	8.46907649802235E-4
52-53	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.103988160715003	8.46907649802235E-4
54-55	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.1161473348300208	8.71105011225156E-4
56-57	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.12984304139539407	9.194997340709979E-4
58-59	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.1437686228942851	9.436970954939189E-4
60-61	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.1572223558454292	9.799931376283004E-4
62-63	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.17175287137989326	9.92091818339761E-4
64-65	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.1853638871802863	9.92091818339761E-4
66-67	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.19974921854621286	9.92091818339761E-4
68-69	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.21510244436905623	9.92091818339761E-4
70-71	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.23199220264225506	0.0010283878604741424
72-73	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.24792616513924853	0.0011735720290116684
74-75	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.26494900890027345	0.00120986807114605
76-77	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.2823953064861995	0.00120986807114605
78-79	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.30201936660018847	0.0012461641132804316
80-81	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.3208933085100668	0.0013187561975491944
82-83	2.4197361422921E-5	1.2098680711460499E-4	0.0	0.3397309543778108	0.0014155456432408784
84-85	2.4197361422921E-5	1.330854878260655E-4	0.0	0.35910094219685906	0.001427644323952339
86-87	2.4197361422921E-5	1.45184168537526E-4	0.0	0.3791242587743262	0.0014881377275096416
88-89	2.4197361422921E-5	1.45184168537526E-4	0.0	0.401869778511872	0.001548631131066944
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5440	0.0	36.34716	1
TACCTGG	5660	0.0	33.820606	2
ACCTGGG	7525	0.0	25.186607	3
GTATCAA	13905	0.0	23.06032	1
GTACATG	11865	0.0	22.688303	1
TACATGG	12220	0.0	21.689812	2
GAGTACA	9600	0.0	20.745615	1
ACATGGG	12320	0.0	20.396152	3
TCAACGC	15845	0.0	19.756601	4
AGTACAT	8995	0.0	19.749823	2
CAACGCA	15975	0.0	19.554005	5
TATCAAC	16420	0.0	19.266102	2
ATCAACG	16620	0.0	18.920399	3
AACGCAG	16805	0.0	18.702137	6
AGAGTAC	12590	0.0	18.534466	10-11
GTGTAGC	2255	0.0	17.959475	1
CCTGGGG	8440	0.0	17.33514	4
GTATATA	980	0.0	17.016228	1
CATGGGG	10155	0.0	16.699652	4
ACGCAGA	19070	0.0	16.456533	7
>>END_MODULE