FastQCFastQC Report
Mon 15 Aug 2016
SRR2921436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049656
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51200.1678877879996957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35230.11552122600057187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.10043755754747422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72200.035.383641
TCAACGC86250.028.9638124
ATCAACG88550.028.263273
CAACGCA88450.028.0879335
TATCAAC89550.027.9476572
GTACCTG32450.027.5190721
AACGCAG93450.026.6753226
GGTATCA46450.026.2607351
TACCTGG36100.024.37882
ACGCAGA107450.023.1098657
GTACATG88050.022.7408491
TACATGG90850.022.0482862
CGCAGAG116200.021.2859238
GAGTACA70400.020.2037771
ACATGGG96900.020.151353
AGTACAT69100.019.5023782
GTATATA8400.019.1794221
AGAGTAC94000.019.1495610-11
GCAGAGT128600.018.8413689
CATGGGG75700.017.741494
Produced by FastQC (version 0.11.5)