##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921436_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3049656
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81750531863266	31.0	31.0	34.0	28.0	34.0
2	30.9360036017177	31.0	31.0	34.0	28.0	34.0
3	31.06919108253521	33.0	31.0	34.0	28.0	34.0
4	34.59296425564064	37.0	35.0	37.0	32.0	37.0
5	34.60146816558982	37.0	35.0	37.0	32.0	37.0
6	34.698206289496255	37.0	35.0	37.0	32.0	37.0
7	34.60278831448531	37.0	35.0	37.0	32.0	37.0
8	34.595004485751836	37.0	35.0	37.0	32.0	37.0
9	36.11407286592324	39.0	37.0	39.0	32.0	39.0
10-11	36.00642039626764	39.0	37.0	39.0	32.0	39.0
12-13	35.86588159451426	39.0	37.0	39.0	31.0	39.0
14-15	36.9578539350012	40.0	37.0	41.0	31.0	41.0
16-17	36.83071074245751	40.0	37.0	41.0	31.0	41.0
18-19	36.67456345896062	40.0	37.0	41.0	30.5	41.0
20-21	36.57305233770629	40.0	37.0	41.0	30.0	41.0
22-23	36.628311684990045	40.0	37.0	41.0	30.0	41.0
24-25	36.52214774387669	40.0	37.0	41.0	30.0	41.0
26-27	36.27810087432812	40.0	36.0	41.0	29.5	41.0
28-29	36.069984122799426	39.0	36.0	41.0	29.0	41.0
30-31	35.836920787131405	39.0	36.0	40.5	27.5	41.0
32-33	35.57998951357136	39.0	35.0	40.0	27.0	41.0
34-35	35.177343116731855	39.0	35.0	40.0	25.5	41.0
36-37	34.813635537909846	38.0	35.0	40.0	24.0	41.0
38-39	34.60601408814634	38.0	34.0	40.0	24.0	41.0
40-41	34.368374990490736	38.0	34.0	40.0	23.5	41.0
42-43	34.10489674901038	38.0	33.5	40.0	22.0	41.0
44-45	33.80121872762042	38.0	33.0	40.0	20.0	41.0
46-47	33.5234572358325	38.0	33.0	40.0	18.5	41.0
48-49	33.32834129488703	38.0	33.0	40.0	16.5	41.0
50-51	32.547990658618545	37.0	32.0	39.0	15.0	40.5
52-53	33.012700448837506	37.5	32.5	39.0	18.0	40.0
54-55	33.46607305873187	38.0	33.0	40.0	18.0	41.0
56-57	33.338032715821065	38.0	33.0	40.0	16.0	41.0
58-59	33.032373815276216	37.0	33.0	40.0	13.5	41.0
60-61	32.604571466421135	36.5	32.0	39.5	8.5	41.0
62-63	32.14963015500764	36.0	31.5	39.0	7.5	40.5
64-65	31.636483098421593	35.5	31.0	39.0	4.0	40.0
66-67	31.172818180148845	35.0	31.0	38.0	2.0	40.0
68-69	30.678772786176538	35.0	30.0	37.5	2.0	40.0
70-71	30.174920220510117	34.5	29.5	37.0	2.0	39.0
72-73	29.643232220289764	34.0	29.0	36.5	2.0	39.0
74-75	29.06946799901366	34.0	28.5	36.0	2.0	38.5
76-77	28.508529158698554	34.0	27.5	35.0	2.0	37.5
78-79	27.967923103458226	33.5	26.5	35.0	2.0	37.0
80-81	27.412386347837263	33.0	26.0	35.0	2.0	36.0
82-83	26.81105934570981	32.5	25.0	35.0	2.0	36.0
84-85	26.223197960688026	32.0	24.5	34.5	2.0	35.0
86-87	25.63289597252936	32.0	22.0	34.0	2.0	35.0
88-89	25.016074108030544	31.0	19.5	34.0	2.0	35.0
90-91	24.307154479062557	31.0	16.5	34.0	2.0	35.0
92-93	23.524038776832533	30.5	4.5	34.0	2.0	35.0
94-95	22.654138696298862	30.0	2.0	34.0	2.0	35.0
96-97	21.777467032347253	29.5	2.0	34.0	2.0	35.0
98	21.15954192866343	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70480.0
3	12571.0
4	9918.0
5	8595.0
6	8098.0
7	8281.0
8	9587.0
9	11054.0
10	12322.0
11	13851.0
12	15964.0
13	19516.0
14	15871.0
15	15717.0
16	17824.0
17	20813.0
18	23549.0
19	21739.0
20	22120.0
21	23907.0
22	26289.0
23	29320.0
24	33262.0
25	37453.0
26	42567.0
27	49298.0
28	58031.0
29	68608.0
30	82364.0
31	100608.0
32	127252.0
33	167220.0
34	231910.0
35	337266.0
36	476608.0
37	543925.0
38	262040.0
39	13858.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.76779693920365	17.40823395286889	13.174713509711616	24.64925559821585
2	15.820964370628923	18.936102432432346	38.97049198184132	26.272441215097412
3	19.859653474605135	22.56988944602266	28.477410489470568	29.09304658990164
4	13.679434716531444	15.026563283347812	35.32431091640703	35.96969108371372
5	15.100384289890625	35.91054652775659	33.26225725929214	15.726811923060641
6	34.46212890845468	33.718239498919765	16.802745598353532	15.01688599427202
7	30.464894720576975	30.79325524067852	19.736766995584897	19.00508304315961
8	27.641736452963663	33.786866368137765	19.216379666318023	19.355017512580556
9	26.856083090919224	16.301542376827943	19.307029079853994	37.535345452398836
10-11	26.374015965597923	25.11497995240264	26.493804684862198	22.017199397137237
12-13	26.480210216488548	22.287850651187387	26.96705323871952	24.26488589360455
14-15	23.661905360052973	23.77257761570408	24.651330739993828	27.91418628424912
16-17	23.952229153138262	26.85981949008573	25.25881068104449	23.929140675731517
18-19	23.87606994086288	26.14496140207203	26.74522414621278	23.233744510852304
20-21	24.4462415448441	25.380934321906302	26.737746947003387	23.435077186246208
22-23	24.24394022131173	25.0645324310036	26.566516863780475	24.12501048390419
24-25	24.037491420713444	25.32487253924403	26.63857194729582	23.999064092746703
26-27	24.01201385117876	25.412114579266586	26.674021884809935	23.901849684744718
28-29	23.97665847544883	25.574700854252008	26.563341570501635	23.88529909979752
30-31	24.209562683746324	25.517295122847543	26.403575834733306	23.869566358672824
32-33	23.990139380480173	25.561720720750277	26.373417659234573	24.07472223953498
34-35	24.167767824137538	25.537764144007525	26.474756530995837	23.819711500859107
36-37	24.275781132006955	25.43969443850682	26.371812150327933	23.912712279158285
38-39	24.180218744710626	25.561074012839345	26.31299452065913	23.945712721790898
40-41	24.293317360662392	25.482656691555505	26.33172996266427	23.892295985117833
42-43	24.218120229825914	25.43580508334817	26.213034243005946	24.133040443819965
44-45	24.177586315780275	25.525621389029823	26.19033917541225	24.10645311977765
46-47	24.293982317284488	25.514322485169057	26.134088045586097	24.05760715196036
48-49	24.269439239194387	25.573546020757366	26.03177287932013	24.125241860728114
50-51	24.11410404820605	25.784175148071824	26.011804196809603	24.089916606912528
52-53	24.24870596284335	25.710080560589095	25.903449737133105	24.137763739434455
54-55	24.18542216934482	25.62271304282987	26.047057476622946	24.14480731120236
56-57	24.204432390398082	25.708339200776713	26.035470467824588	24.051757941000616
58-59	24.32521632247182	25.583750855596428	25.95993302938504	24.131099792546717
60-61	24.24881997466169	25.67710074847604	26.09043112721191	23.98364814965036
62-63	24.110864077380135	25.734273091199967	26.04409769212085	24.110765139299048
64-65	24.17342092385671	25.646616736044077	26.010549192317672	24.169413147781547
66-67	24.208397261403945	25.64790893343232	25.9807803349006	24.162913470263135
68-69	24.173508952272027	25.647564331006016	26.02421570538284	24.154711011339117
70-71	24.177286974848293	25.489794401469158	26.00648120481916	24.326437418863396
72-73	24.24065114491817	25.591770918159025	25.909279203957013	24.258298732965795
74-75	24.08703940934743	25.82212087185043	25.794427442886626	24.296412275915515
76-77	24.269020879468524	25.569901402509753	25.799374538648106	24.361703179373617
78-79	24.175302743224318	25.61701952384875	25.95844797759288	24.24922975533405
80-81	24.229662410504357	25.686684485203493	25.83520890375469	24.248444200537456
82-83	24.350476859667012	25.568579927797074	25.82080240562173	24.260140806914183
84-85	24.2410954318788	25.63646181459732	25.771522777350214	24.350919976173657
86-87	24.329714272253092	25.586443898952272	25.859508473881032	24.224333354913604
88-89	24.17228571805215	25.457960926995177	25.89337973582219	24.47637361913048
90-91	24.22542200396093	25.554067859690015	25.85328748659962	24.36722264974944
92-93	24.34807520532604	25.611612457253603	25.83646864583165	24.20384369158871
94-95	24.431037999560417	25.428272316178273	25.838478554502874	24.30221112975843
96-97	24.351906067526652	25.4007514676949	25.933560054315503	24.31378241046295
98	24.33010451512868	25.469323042058768	25.72272799609102	24.477844446721534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	882.0
1	664.5
2	713.5
3	973.5
4	794.0
5	718.0
6	1145.5
7	2210.5
8	3127.5
9	3046.0
10	2302.0
11	1753.0
12	1775.0
13	1990.5
14	2235.0
15	2467.5
16	2677.5
17	2797.5
18	2774.5
19	2714.0
20	2616.5
21	2494.5
22	2544.0
23	2718.0
24	2997.5
25	3612.5
26	4570.0
27	6071.0
28	8108.5
29	10806.0
30	14569.5
31	19217.5
32	24816.5
33	30709.5
34	37019.0
35	43780.5
36	50652.5
37	58091.0
38	65562.0
39	72837.0
40	80256.5
41	88065.0
42	96610.0
43	105011.5
44	112736.5
45	120394.0
46	129337.0
47	137010.0
48	140489.0
49	139783.5
50	138132.0
51	137387.0
52	135595.0
53	133885.5
54	130496.5
55	123851.0
56	114990.5
57	105853.0
58	96388.0
59	85198.5
60	73887.0
61	62191.0
62	50029.0
63	38086.5
64	27866.5
65	20938.0
66	15924.0
67	12066.5
68	9065.5
69	6801.0
70	5011.0
71	3726.0
72	2859.5
73	2086.0
74	1566.0
75	1144.5
76	763.5
77	462.5
78	267.5
79	163.5
80	100.5
81	52.0
82	30.0
83	22.0
84	18.0
85	15.0
86	12.0
87	8.5
88	7.0
89	8.0
90	5.0
91	3.5
92	3.5
93	2.5
94	2.0
95	3.0
96	3.5
97	2.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5118282193139161
2	0.05367818534287146
3	0.06276117699832374
4	0.060334673812390646
5	0.07269672382721198
6	0.07082766056237162
7	0.04859564488584942
8	0.02311736143355185
9	0.015280411954659805
10-11	0.01762493868160868
12-13	0.013722859233959503
14-15	0.018461098563247787
16-17	0.016919941134344335
18-19	0.059810024474891595
20-21	0.09091189301350709
22-23	0.10884834223925584
24-25	0.07691031381900122
26-27	0.09310886211428437
28-29	0.09499432067092156
30-31	0.06479419318113257
32-33	0.0382338204702432
34-35	0.42885164753008204
36-37	0.6715019661233923
38-39	0.6170204114824754
40-41	0.6113968263961574
42-43	0.5835412256333173
44-45	0.5678181408001427
46-47	0.5566857376700848
48-49	0.5463075179626817
50-51	0.5604074689079687
52-53	0.645990892087501
54-55	0.6146758847555265
56-57	0.5701298769434979
58-59	0.5470780966771335
60-61	0.5605222359505465
62-63	0.5724088225032594
64-65	0.5918536385743179
66-67	0.6199387734223138
68-69	0.5708512697825591
70-71	0.5183043595736699
72-73	0.4999580280530001
74-75	0.48005414381162986
76-77	0.49500664993035276
78-79	0.4887764390475516
80-81	0.4853498230620109
82-83	0.5059587048506455
84-85	0.4708727804053965
86-87	0.47469288339406146
88-89	0.5028763899928386
90-91	0.4840381997182633
92-93	0.4789064733858507
94-95	0.4906455023123919
96-97	0.485136684268652
98	0.4785129863827265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3049656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.562104994100075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18890548976465	45.37971588166929
2	13.867677144511447	16.519760842113456
3	4.387228189314354	7.839376381350513
4	1.906086051789848	4.541219901779864
5	0.9775289119162357	2.9111839843161613
6	0.5837218812995947	2.086062238879205
7	0.39850191962423587	1.6614929223906414
8	0.29013091685835635	1.382464650556155
9	0.21783076475910235	1.167701299337409
>10	1.1037597786388567	11.703308936091245
>50	0.05986764723780516	2.3869318344617296
>100	0.018098515030621456	1.794312079848677
>500	3.866270654862584E-4	0.16040871058165995
>1k	2.2092975152507676E-4	0.2976246110989411
>5k	5.523243788126919E-5	0.1684357255252104
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5120	0.1678877879996957	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3523	0.11552122600057187	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3063	0.10043755754747422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.558116718738113E-5	0.0
5	3.279058359369057E-5	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
6	3.279058359369057E-5	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
7	3.279058359369057E-5	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
8	3.279058359369057E-5	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
9	3.279058359369057E-5	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
10-11	6.558116718738113E-5	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
12-13	9.83717507810717E-5	4.9185875390535854E-5	0.0	8.197645898422641E-5	4.9185875390535854E-5
14-15	9.83717507810717E-5	1.1476704257791699E-4	0.0	1.1476704257791699E-4	9.837175078107171E-5
16-17	1.6395291796845283E-4	1.6395291796845283E-4	0.0	1.9674350156214342E-4	1.3116233437476227E-4
18-19	1.967435015621434E-4	2.2953408515583398E-4	0.0	2.6232466874952454E-4	1.3116233437476227E-4
20-21	1.967435015621434E-4	2.2953408515583398E-4	0.0	2.6232466874952454E-4	1.4755762617160755E-4
22-23	1.967435015621434E-4	2.2953408515583398E-4	0.0	3.1151054414006035E-4	1.803482097652981E-4
24-25	2.4592937695267923E-4	2.2953408515583398E-4	0.0	4.918587539053585E-4	1.967435015621434E-4
26-27	2.951152523432151E-4	2.2953408515583398E-4	0.0	7.377881308580378E-4	2.2953408515583398E-4
28-29	2.951152523432151E-4	2.6232466874952454E-4	0.0	0.001787086805856136	2.2953408515583398E-4
30-31	3.1151054414006035E-4	2.6232466874952454E-4	0.0	0.005820328587880076	2.4592937695267923E-4
32-33	3.2790583593690566E-4	2.6232466874952454E-4	0.0	0.013263791063647834	2.6232466874952454E-4
34-35	3.2790583593690566E-4	2.951152523432151E-4	0.0	0.022904222640192862	2.6232466874952454E-4
36-37	3.2790583593690566E-4	2.951152523432151E-4	0.0	0.037135335919854566	2.6232466874952454E-4
38-39	3.2790583593690566E-4	2.951152523432151E-4	0.0	0.060121535019031655	2.6232466874952454E-4
40-41	3.2790583593690566E-4	2.951152523432151E-4	0.0	0.0830585482428182	2.6232466874952454E-4
42-43	3.2790583593690566E-4	3.2790583593690566E-4	0.0	0.10556928387988679	2.7871996054636985E-4
44-45	4.098822949211321E-4	3.2790583593690566E-4	0.0	0.1316869837122613	3.2790583593690566E-4
46-47	4.262775867179774E-4	3.2790583593690566E-4	0.0	0.16073944077627117	3.606964195305962E-4
48-49	4.262775867179774E-4	3.7709171132744153E-4	0.0	0.1894312014207504	3.606964195305962E-4
50-51	4.426728785148227E-4	4.918587539053585E-4	0.0	0.21759831272773061	3.606964195305962E-4
52-53	4.5906817031166796E-4	4.918587539053585E-4	0.0	0.2458637957854919	3.9348700312428684E-4
54-55	4.5906817031166796E-4	5.574399210927397E-4	0.0	0.2738997447580973	4.262775867179774E-4
56-57	4.5906817031166796E-4	6.230210882801208E-4	0.0	0.3024603430682018	4.262775867179774E-4
58-59	4.5906817031166796E-4	6.230210882801208E-4	0.0	0.3335786068986141	4.262775867179774E-4
60-61	4.5906817031166796E-4	7.541834226548831E-4	0.0	0.364746056604417	4.262775867179774E-4
62-63	4.5906817031166796E-4	7.541834226548831E-4	0.0	0.3957823439758451	4.5906817031166796E-4
64-65	4.5906817031166796E-4	7.705787144517283E-4	0.0	0.4264743302195395	4.5906817031166796E-4
66-67	4.5906817031166796E-4	7.869740062485736E-4	0.0	0.4609372335765083	5.246493374990491E-4
68-69	5.246493374990491E-4	7.869740062485736E-4	0.0	0.4946787440944159	5.246493374990491E-4
70-71	5.246493374990491E-4	8.033692980454188E-4	0.0	0.529846644998649	5.246493374990491E-4
72-73	5.246493374990491E-4	8.197645898422642E-4	0.0	0.5653916376142096	6.066257964832756E-4
74-75	5.246493374990491E-4	8.197645898422642E-4	0.0	0.6019039524457841	6.230210882801208E-4
76-77	5.246493374990491E-4	8.197645898422642E-4	0.0	0.6387769636968891	6.394163800769661E-4
78-79	5.246493374990491E-4	8.197645898422642E-4	0.0	0.6776010146718188	6.558116718738113E-4
80-81	5.246493374990491E-4	8.197645898422642E-4	0.0	0.7181301759936203	6.886022554675019E-4
82-83	5.246493374990491E-4	8.197645898422642E-4	0.0	0.7601513088689347	8.525551734359548E-4
84-85	5.246493374990491E-4	8.197645898422642E-4	0.0	0.8001230302696436	8.525551734359548E-4
86	5.246493374990491E-4	8.853457570296453E-4	0.0	0.8307166447625568	8.853457570296453E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7220	0.0	35.38364	1
TCAACGC	8625	0.0	28.963812	4
ATCAACG	8855	0.0	28.26327	3
CAACGCA	8845	0.0	28.087933	5
TATCAAC	8955	0.0	27.947657	2
GTACCTG	3245	0.0	27.519072	1
AACGCAG	9345	0.0	26.675322	6
GGTATCA	4645	0.0	26.260735	1
TACCTGG	3610	0.0	24.3788	2
ACGCAGA	10745	0.0	23.109865	7
GTACATG	8805	0.0	22.740849	1
TACATGG	9085	0.0	22.048286	2
CGCAGAG	11620	0.0	21.285923	8
GAGTACA	7040	0.0	20.203777	1
ACATGGG	9690	0.0	20.15135	3
AGTACAT	6910	0.0	19.502378	2
GTATATA	840	0.0	19.179422	1
AGAGTAC	9400	0.0	19.14956	10-11
GCAGAGT	12860	0.0	18.841368	9
CATGGGG	7570	0.0	17.74149	4
>>END_MODULE