FastQCFastQC Report
Mon 15 Aug 2016
SRR2921436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049656
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54580.17897100525436313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37840.12407956831852511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32240.10571684150605838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74400.032.9172021
GGTATCA49100.030.6646751
GTACCTG30850.029.035911
TCAACGC90700.026.7086774
TACCTGG32550.026.4116572
CAACGCA92600.026.1451975
TATCAAC94350.025.825192
ATCAACG95300.025.3692283
GTACATG90050.024.8683451
AACGCAG98350.024.8101066
TACATGG94100.023.0167122
ACGCAGA111550.021.917547
GAGTACA69600.021.8380661
ACATGGG99750.021.5708683
AGTACAT68350.020.6388762
CGCAGAG118500.020.6320788
AGAGTAC96700.020.20164910-11
CATGGGG72400.019.9445634
ACCTGGG42750.019.7772643
TATCACG2552.6800808E-718.6263562
Produced by FastQC (version 0.11.5)