##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3049656
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06284085811646	33.0	31.0	34.0	30.0	34.0
2	32.18943808744331	34.0	31.0	34.0	30.0	34.0
3	32.30963229951181	34.0	31.0	34.0	30.0	34.0
4	35.82492713932326	37.0	35.0	37.0	35.0	37.0
5	35.65195254809067	37.0	35.0	37.0	35.0	37.0
6	35.6907392178003	37.0	35.0	37.0	35.0	37.0
7	35.62895421647556	37.0	35.0	37.0	35.0	37.0
8	35.58911332950339	37.0	35.0	37.0	35.0	37.0
9	37.2436648592497	39.0	38.0	39.0	34.0	39.0
10-11	37.16195826676845	39.0	37.0	39.0	34.0	39.0
12-13	37.057343352824056	39.0	37.0	39.0	34.0	39.0
14-15	38.39332255834756	40.0	38.0	41.0	34.0	41.0
16-17	38.30983363369508	40.0	38.0	41.0	34.0	41.0
18-19	38.22373129953018	40.0	38.0	41.0	33.5	41.0
20-21	38.12817298082145	40.0	38.0	41.0	33.5	41.0
22-23	38.09491316397653	40.0	38.0	41.0	33.5	41.0
24-25	38.15011086496313	40.0	38.0	41.0	34.0	41.0
26-27	38.051138718596455	40.0	38.0	41.0	33.0	41.0
28-29	37.93158129966134	40.0	38.0	41.0	33.0	41.0
30-31	37.800678666708635	40.0	38.0	41.0	33.0	41.0
32-33	37.642221614503406	40.0	38.0	41.0	33.0	41.0
34-35	37.501042412652446	40.0	37.5	41.0	32.0	41.0
36-37	37.406465188204834	40.0	37.0	41.0	32.0	41.0
38-39	37.37740141838948	40.0	37.0	41.0	32.0	41.0
40-41	37.22597401149507	40.0	37.0	41.0	32.0	41.0
42-43	37.007495435550766	40.0	37.0	41.0	31.0	41.0
44-45	36.81016891741233	40.0	36.5	41.0	31.0	41.0
46-47	36.546873155529674	39.5	36.0	41.0	30.0	41.0
48-49	36.22916010854995	39.0	35.5	40.5	30.0	41.0
50-51	35.97745352262682	39.0	35.0	40.0	29.0	41.0
52-53	35.69593750901741	39.0	35.0	40.0	28.5	41.0
54-55	35.398317056087635	38.0	35.0	40.0	28.0	41.0
56-57	35.04331734464478	38.0	34.0	40.0	27.5	41.0
58-59	34.68719094874963	38.0	34.0	40.0	26.5	41.0
60-61	34.29115136264549	37.0	34.0	40.0	26.0	41.0
62-63	33.88413676821254	36.5	33.0	39.0	26.0	41.0
64-65	33.405754616258356	36.0	33.0	39.0	24.5	40.0
66-67	32.90234849438757	35.0	32.0	38.5	23.0	40.0
68-69	32.4216462446912	35.0	32.0	38.0	22.0	40.0
70-71	31.89518145653149	35.0	31.0	37.0	20.5	39.0
72-73	31.37821511672136	34.5	31.0	36.5	19.5	39.0
74-75	30.878163963410955	34.0	30.0	36.0	18.0	38.5
76-77	29.51013720891799	32.5	28.5	35.0	17.5	36.5
78-79	30.443630035649925	34.0	30.5	35.0	18.0	37.0
80-81	30.48412624243521	34.0	31.0	35.0	18.0	37.0
82-83	30.173551377598	34.0	30.0	35.0	15.5	36.0
84-85	29.716299477711587	34.0	30.0	35.0	8.5	36.0
86-87	29.23649191908858	34.0	29.0	35.0	4.5	35.5
88-89	28.72062865451054	34.0	29.0	35.0	2.0	35.0
90-91	28.295830251018476	33.0	28.5	35.0	2.0	35.0
92-93	27.909064005907553	33.0	27.0	35.0	2.0	35.0
94-95	27.475986144010996	33.0	27.0	35.0	2.0	35.0
96-97	26.795403317620085	33.0	25.5	34.0	2.0	35.0
98-99	25.87850580524492	32.0	24.5	34.0	2.0	35.0
100-101	22.27828417369041	28.5	10.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16654.0
3	8771.0
4	5239.0
5	3891.0
6	3537.0
7	3635.0
8	4225.0
9	5034.0
10	5960.0
11	7265.0
12	8390.0
13	10212.0
14	11261.0
15	12992.0
16	14905.0
17	18131.0
18	18623.0
19	15395.0
20	14778.0
21	15413.0
22	16511.0
23	18431.0
24	21229.0
25	24960.0
26	29540.0
27	36152.0
28	44323.0
29	54101.0
30	66185.0
31	84223.0
32	108770.0
33	146898.0
34	211683.0
35	331415.0
36	540501.0
37	729716.0
38	366621.0
39	14085.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.972188416797366	17.82402952110133	13.567638650770386	24.63614341133092
2	15.921008795746145	19.24935140225652	38.79847431972655	26.031165482270787
3	19.934624876782188	22.607541356258352	28.678318059681036	28.779515707278424
4	13.817385383940216	15.292509864076715	35.30130345263193	35.58880129935115
5	15.158472751382867	35.85323649570556	33.30247724240329	15.685813510508288
6	34.51039041064877	33.853143700491586	16.67092122161069	14.965544667248961
7	30.545385573841287	30.707230208898373	19.71826826240489	19.029115954855456
8	27.643996655809318	33.81479656665716	19.2336590757127	19.307547701820823
9	26.723130093441995	16.288872624154443	19.363202825250013	37.62479445715355
10-11	26.368965466310655	25.108288052620264	26.521932494949514	22.000813986119567
12-13	26.431167453476572	22.24467463224856	27.07908350403766	24.245074410237205
14-15	23.602504546524667	23.786170197930705	24.75041896932316	27.860906286221464
16-17	23.90339108699788	26.817075490884278	25.40153120469202	23.878002217425816
18-19	23.82637522435262	26.04273225447258	26.970452782661336	23.160439738513467
20-21	24.36096903863156	25.284819629131377	26.922767218941257	23.4314441132958
22-23	24.159790395373847	25.02017447807149	26.763282187477657	24.056752939077015
24-25	24.02254977516976	25.200651373804035	26.847766384512926	23.929032466513274
26-27	23.898813721085066	25.359786555068332	26.928514970334827	23.812884753511774
28-29	23.86664167452591	25.560433177427637	26.76613997861893	23.806785169427528
30-31	24.105127486654197	25.422516633167234	26.637071086940733	23.835284793237832
32-33	23.929829186835946	25.43868235513247	26.581574070384946	24.049914387646638
34-35	24.08374834374323	25.42682275966114	26.71319789671582	23.776230999879814
36-37	24.151928456415014	25.3963329649464	26.57831392469821	23.873424653940372
38-39	24.08450196119587	25.491151661441187	26.529175248265098	23.89517112909785
40-41	24.179692483688964	25.387543952805647	26.585113392581	23.84765017092439
42-43	24.153547244675444	25.382744995163222	26.432998311226246	24.030709448935088
44-45	24.06772029165501	25.4781142318738	26.405204086685803	24.048961389785383
46-47	24.163588830118183	25.49244018184627	26.359296268976625	23.98467471905893
48-49	24.22546355776527	25.56052238252919	26.17872510383445	24.0352889558711
50-51	24.034695556347323	25.751863580175964	26.16761211363596	24.04582874984075
52-53	24.18282243271373	25.62231479592941	26.074540707546152	24.12032206381071
54-55	24.159798133158006	25.553371251682634	26.11550613454182	24.171324480617535
56-57	24.15604797647118	25.663473777080547	26.141349677237834	24.039128569210437
58-59	24.296276038733406	25.557369445426808	26.03977452239471	24.106579993445077
60-61	24.245261765777418	25.604812065974077	26.208140282623287	23.941785885625222
62-63	24.0390668286963	25.654258041316247	26.21511975210844	24.091555377879022
64-65	24.174121661648574	25.586188792757287	26.07393000511073	24.165759540483407
66-67	24.154837533005864	25.5863230301083	26.004379514395797	24.25445992249004
68-69	24.13760745763624	25.65483496613491	26.06555354647521	24.14200402975364
70-71	24.164182905195002	25.482285890250783	26.070075277772176	24.28345592678204
72-73	24.2738776693039	25.554238241302922	25.945026284054755	24.22685780533842
74-75	24.019442603164574	25.814596157704106	25.874370089544435	24.29159114958689
76-77	24.205043097670234	25.564052735330716	25.90130094846948	24.32960321852957
78-79	24.134043947205072	25.637953488494205	26.016560315789008	24.21144224851172
80-81	24.165005818980504	25.70437873362715	25.93475984732885	24.195855600063503
82-83	24.22616614015313	25.620947317650923	25.940053387701738	24.21283315449421
84-85	24.182314695303944	25.663105865229163	25.82841805207112	24.32616138739577
86-87	24.184277577568604	25.66225790640191	25.9329260425459	24.220538473483582
88-89	24.10951108790039	25.572610820482538	25.917342794340144	24.400535297276924
90-91	24.11264129905164	25.6708216391281	25.899244435046757	24.3172926267735
92-93	24.272543805502732	25.654254964027643	25.964234493928934	24.108966736540694
94-95	24.2424217580708	25.647097056852065	25.860221788765486	24.250259396311655
96-97	24.162297054898062	25.498316100830635	26.01156922350441	24.327817620766886
98-99	24.159624836542363	25.61005464301672	25.868569812295295	24.36175070814562
100-101	24.506104874471514	25.13214452315737	26.01036430847632	24.351386293894798
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	537.0
1	420.0
2	624.0
3	881.5
4	618.0
5	483.0
6	900.0
7	1972.0
8	2883.0
9	2758.0
10	1853.0
11	1121.0
12	977.5
13	1042.0
14	1114.5
15	1179.0
16	1255.0
17	1339.0
18	1468.0
19	1579.5
20	1692.5
21	1820.5
22	2031.0
23	2306.5
24	2673.0
25	3204.5
26	3980.0
27	5150.0
28	6804.5
29	9144.5
30	12345.0
31	16209.5
32	20908.5
33	26392.0
34	32370.5
35	38545.0
36	45466.5
37	52509.5
38	59225.0
39	66028.5
40	73883.0
41	81726.0
42	88928.0
43	97108.0
44	104918.0
45	112556.0
46	120509.5
47	129116.0
48	137345.5
49	140430.5
50	137778.0
51	136191.0
52	134506.5
53	131940.5
54	129582.5
55	123731.5
56	114466.0
57	104268.5
58	95641.0
59	85814.5
60	73951.5
61	62368.5
62	50938.0
63	38524.5
64	27756.0
65	20562.5
66	15496.5
67	12002.5
68	9160.0
69	6825.5
70	5074.0
71	3772.5
72	2757.0
73	2061.0
74	1560.0
75	1106.5
76	769.0
77	457.5
78	246.5
79	121.0
80	72.0
81	40.5
82	22.0
83	12.0
84	5.5
85	4.0
86	3.0
87	1.0
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33456232440642486
2	0.0
3	0.005836723879676921
4	0.06495814609910101
5	4.918587539053586E-4
6	0.06367931333894708
7	0.0750576458459577
8	0.10424126524434232
9	0.09332200090764335
10-11	0.09578129467717014
12-13	0.09722408035529254
14-15	0.06553198131199059
16-17	0.03528266794681105
18-19	0.038119053427665285
20-21	0.027560485510496925
22-23	0.04098822949211321
24-25	0.0335283717245486
26-27	0.020526905329650295
28-29	0.02287143205659917
30-31	0.003328244234759593
32-33	0.005066145165225193
34-35	0.007689391852720438
36-37	0.010689730251543125
38-39	0.016264129462470522
40-41	0.021215507585117796
42-43	0.0034758018609312
44-45	0.014329485030442778
46-47	0.02318294260073923
48-49	0.0029511525234321508
50-51	0.007099161348034008
52-53	0.002426503185933102
54-55	0.003967660614836559
56-57	0.0039184747394460226
58-59	0.001328018635544468
60-61	0.005082540457022038
62-63	0.013755649817553194
64-65	0.006246606174598053
66-67	0.03752882292297885
68-69	0.05990839622567267
70-71	0.011460308965994852
72-73	0.03097070620424074
74-75	0.04933343301670746
76-77	0.3199869099990294
78-79	0.07973030400805861
80-81	0.03318407059681486
82-83	0.027101417340185254
84-85	0.018903771441762612
86-87	0.12050539470681283
88-89	0.5435039230654212
90-91	0.130768847371638
92-93	0.05087459044561092
94-95	0.008787876403109072
96-97	0.006099048548426445
98-99	0.010443800874590445
100-101	0.008148460023032105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3049656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.67962526289503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7157776111245	41.78182650961444
2	15.169643805356102	17.196194525183618
3	4.922475252394629	8.370121580148068
4	2.082675782176375	4.721811315114549
5	1.0811603436800166	3.0639881564443074
6	0.6796830461922815	2.311450821343289
7	0.450796807878501	1.7885695898183906
8	0.3173281303638347	1.4388831611517803
9	0.23099544071622122	1.1783461515509435
>10	1.2553534375609061	12.690808876133802
>50	0.0703334187961382	2.6798168196274474
>100	0.02308176434003679	2.123824151338833
>500	4.0550966218393173E-4	0.15970690853669586
>1k	2.317198066809415E-4	0.31544084476464157
>5k	5.7929951670235374E-5	0.17921058922925093
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5458	0.17897100525436313	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3784	0.12407956831852511	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3224	0.10571684150605838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.279058359369057E-5	0.0
4	0.0	0.0	0.0	6.558116718738113E-5	0.0
5	0.0	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
6	0.0	3.279058359369057E-5	0.0	6.558116718738113E-5	0.0
7	0.0	3.279058359369057E-5	0.0	9.83717507810717E-5	0.0
8	0.0	6.558116718738113E-5	0.0	9.83717507810717E-5	0.0
9	0.0	6.558116718738113E-5	0.0	9.83717507810717E-5	0.0
10-11	3.279058359369057E-5	6.558116718738113E-5	0.0	2.2953408515583398E-4	0.0
12-13	6.558116718738113E-5	9.83717507810717E-5	0.0	4.098822949211321E-4	3.279058359369057E-5
14-15	6.558116718738113E-5	9.83717507810717E-5	0.0	4.426728785148227E-4	3.279058359369057E-5
16-17	8.197645898422641E-5	1.1476704257791699E-4	0.0	4.5906817031166796E-4	3.279058359369057E-5
18-19	9.83717507810717E-5	1.3116233437476227E-4	0.0	4.918587539053585E-4	3.279058359369057E-5
20-21	9.83717507810717E-5	1.3116233437476227E-4	0.0	4.918587539053585E-4	3.279058359369057E-5
22-23	9.83717507810717E-5	1.3116233437476227E-4	0.0	6.722069636706566E-4	4.9185875390535854E-5
24-25	9.83717507810717E-5	1.4755762617160755E-4	0.0	8.689504652328E-4	6.558116718738113E-5
26-27	9.83717507810717E-5	1.6395291796845283E-4	0.0	0.0011312751339823245	6.558116718738113E-5
28-29	9.83717507810717E-5	1.6395291796845283E-4	0.0	0.002213364392574113	6.558116718738113E-5
30-31	9.83717507810717E-5	1.6395291796845283E-4	0.0	0.006099048548426446	6.558116718738113E-5
32-33	9.83717507810717E-5	1.6395291796845283E-4	0.0	0.013493325148803668	9.83717507810717E-5
34-35	1.1476704257791699E-4	1.6395291796845283E-4	0.0	0.023232128476129765	1.3116233437476227E-4
36-37	1.3116233437476227E-4	1.803482097652981E-4	0.0	0.03761079938196308	1.3116233437476227E-4
38-39	1.3116233437476227E-4	2.2953408515583398E-4	0.0	0.0604658361467654	1.3116233437476227E-4
40-41	1.3116233437476227E-4	2.6232466874952454E-4	0.0	0.0834520352459425	1.3116233437476227E-4
42-43	1.3116233437476227E-4	3.2790583593690566E-4	0.0	0.10606114263379214	1.3116233437476227E-4
44-45	1.3116233437476227E-4	3.2790583593690566E-4	0.0	0.1318509366302298	1.3116233437476227E-4
46-47	1.3116233437476227E-4	3.4430112773375097E-4	0.0	0.16065746431728695	1.3116233437476227E-4
48-49	1.3116233437476227E-4	3.606964195305962E-4	0.0	0.1891688767520009	1.4755762617160755E-4
50-51	1.3116233437476227E-4	3.770917113274415E-4	0.0	0.21700808222304419	1.967435015621434E-4
52-53	1.6395291796845283E-4	4.426728785148226E-4	0.0	0.245109612362837	1.967435015621434E-4
54-55	1.6395291796845283E-4	5.410446292958943E-4	0.0	0.27286684137489603	1.967435015621434E-4
56-57	1.6395291796845283E-4	5.574399210927397E-4	0.0	0.3013126726424226	2.2953408515583398E-4
58-59	1.967435015621434E-4	5.902305046864302E-4	0.0	0.33213582122049173	2.6232466874952454E-4
60-61	2.2953408515583398E-4	6.558116718738114E-4	0.0	0.36289338863137355	2.6232466874952454E-4
62-63	2.2953408515583398E-4	6.886022554675019E-4	0.0	0.39363456075045844	2.6232466874952454E-4
64-65	2.2953408515583398E-4	6.886022554675019E-4	0.0	0.42380189765665377	2.6232466874952454E-4
66-67	2.2953408515583398E-4	7.541834226548831E-4	0.0	0.4580188716366699	2.7871996054636985E-4
68-69	2.2953408515583398E-4	7.541834226548831E-4	0.0	0.49151445277762473	2.951152523432151E-4
70-71	2.2953408515583398E-4	7.541834226548831E-4	0.0	0.5257970079248282	2.951152523432151E-4
72-73	2.2953408515583398E-4	7.541834226548831E-4	0.0	0.5604566547833592	4.426728785148227E-4
74-75	2.2953408515583398E-4	7.869740062485736E-4	0.0	0.596821411988762	4.5906817031166796E-4
76-77	2.2953408515583398E-4	8.197645898422642E-4	0.0	0.633071402151587	4.5906817031166796E-4
78-79	2.2953408515583398E-4	8.197645898422642E-4	0.0	0.6714363849562048	4.5906817031166796E-4
80-81	2.2953408515583398E-4	8.197645898422642E-4	0.0	0.711473687524101	5.082540457022038E-4
82-83	2.2953408515583398E-4	8.525551734359547E-4	0.0	0.7530193569373069	6.558116718738113E-4
84-85	2.2953408515583398E-4	8.853457570296453E-4	0.0	0.7920073608302052	6.558116718738113E-4
86-87	2.2953408515583398E-4	8.853457570296453E-4	0.0	0.8330775667813026	6.722069636706566E-4
88-89	2.2953408515583398E-4	8.853457570296453E-4	0.0	0.8775088075507533	6.886022554675019E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7440	0.0	32.917202	1
GGTATCA	4910	0.0	30.664675	1
GTACCTG	3085	0.0	29.03591	1
TCAACGC	9070	0.0	26.708677	4
TACCTGG	3255	0.0	26.411657	2
CAACGCA	9260	0.0	26.145197	5
TATCAAC	9435	0.0	25.82519	2
ATCAACG	9530	0.0	25.369228	3
GTACATG	9005	0.0	24.868345	1
AACGCAG	9835	0.0	24.810106	6
TACATGG	9410	0.0	23.016712	2
ACGCAGA	11155	0.0	21.91754	7
GAGTACA	6960	0.0	21.838066	1
ACATGGG	9975	0.0	21.570868	3
AGTACAT	6835	0.0	20.638876	2
CGCAGAG	11850	0.0	20.632078	8
AGAGTAC	9670	0.0	20.201649	10-11
CATGGGG	7240	0.0	19.944563	4
ACCTGGG	4275	0.0	19.777264	3
TATCACG	255	2.6800808E-7	18.626356	2
>>END_MODULE