FastQCFastQC Report
Mon 15 Aug 2016
SRR2921435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4520728
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48760.10785873425696038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127150.028.9241281
GTACATG155750.024.5585561
TATCAAC152000.023.8251482
TCAACGC151450.023.7603324
TACATGG158350.023.5934622
CAACGCA154250.023.3290255
ATCAACG156700.022.905773
AACGCAG161500.022.2763216
ACATGGG164800.022.1415483
GTATAGG10200.021.6606241
GTACCTG43900.021.4940991
TACCTGG46500.020.11082
GGTATCA90050.019.934751
GAGTACA120100.019.9292161
ACGCAGA182050.019.5818427
AGTACAT115450.018.741322
CGCAGAG190850.018.6971478
AGAGTAC136350.018.47716110-11
CATGGGG130050.018.3997634
GTATATG13400.018.2054311
Produced by FastQC (version 0.11.5)