##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921435_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4520728
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.537166359046594	31.0	31.0	34.0	27.0	34.0
2	30.64491913691777	31.0	31.0	34.0	27.0	34.0
3	30.779067442234968	31.0	31.0	34.0	27.0	34.0
4	34.283288886214784	37.0	35.0	37.0	30.0	37.0
5	34.28140688844805	37.0	35.0	37.0	30.0	37.0
6	34.378187760909306	37.0	35.0	37.0	31.0	37.0
7	34.272387323457636	37.0	35.0	37.0	30.0	37.0
8	34.2553126399111	37.0	35.0	37.0	30.0	37.0
9	35.734851997288935	39.0	37.0	39.0	30.0	39.0
10-11	35.618465433001056	39.0	37.0	39.0	30.0	39.0
12-13	35.47274377047236	39.0	35.5	39.0	30.0	39.0
14-15	36.52494830478631	40.0	37.0	41.0	30.0	41.0
16-17	36.388311572826325	40.0	36.5	41.0	30.0	41.0
18-19	36.217155290032935	40.0	36.0	41.0	29.0	41.0
20-21	36.10933338612719	39.0	36.0	41.0	28.5	41.0
22-23	36.15338491942006	40.0	36.5	41.0	28.5	41.0
24-25	36.036131791162845	40.0	36.0	41.0	28.0	41.0
26-27	35.78408035166018	39.0	36.0	41.0	27.0	41.0
28-29	35.56060583605118	39.0	36.0	40.5	27.0	41.0
30-31	35.325263873429236	39.0	35.0	40.0	25.5	41.0
32-33	35.06826312045317	39.0	35.0	40.0	25.0	41.0
34-35	34.660056190064964	38.0	34.5	40.0	23.5	41.0
36-37	34.29892596944563	38.0	34.0	40.0	22.5	41.0
38-39	34.076589102463146	38.0	33.5	40.0	20.5	41.0
40-41	33.822838157925005	38.0	33.0	40.0	18.5	41.0
42-43	33.545017638751986	38.0	33.0	40.0	17.0	41.0
44-45	33.242026726668804	38.0	33.0	40.0	15.0	41.0
46-47	32.966485707611696	38.0	32.0	40.0	13.5	41.0
48-49	32.766147178065125	38.0	32.0	40.0	9.0	41.0
50-51	31.99868516752169	36.0	31.0	39.0	10.0	40.0
52-53	32.485966419567816	37.0	32.0	39.0	9.0	40.0
54-55	32.926447908389974	38.0	33.0	40.0	8.5	41.0
56-57	32.781998386100646	37.0	32.5	40.0	8.0	41.0
58-59	32.45824123902168	37.0	32.0	40.0	6.5	41.0
60-61	32.01000071227466	36.0	31.0	39.0	2.0	41.0
62-63	31.534675388565734	35.5	31.0	39.0	2.0	40.0
64-65	30.998381234172903	35.0	30.5	38.5	2.0	40.0
66-67	30.513195994096527	35.0	30.0	38.0	2.0	40.0
68-69	29.999194488144386	34.5	29.0	37.5	2.0	39.5
70-71	29.482219677892587	34.0	29.0	37.0	2.0	39.0
72-73	28.93198783470273	34.0	28.0	36.0	2.0	39.0
74-75	28.341816074756103	34.0	27.0	35.5	2.0	38.0
76-77	27.75821560155798	33.0	26.0	35.0	2.0	37.0
78-79	27.200973383048037	33.0	26.0	35.0	2.0	36.5
80-81	26.624785653992014	32.5	25.0	35.0	2.0	36.0
82-83	26.00606240853243	32.0	23.5	34.5	2.0	35.5
84-85	25.388712061420197	31.5	20.5	34.0	2.0	35.0
86-87	24.77435647532875	31.0	18.5	34.0	2.0	35.0
88-89	24.126369248492722	31.0	13.5	34.0	2.0	35.0
90-91	23.386415307446057	30.5	4.5	34.0	2.0	35.0
92-93	22.573712021603598	30.0	2.0	34.0	2.0	35.0
94-95	21.65610549893734	29.0	2.0	34.0	2.0	35.0
96-97	20.753306104680483	29.0	2.0	33.5	2.0	34.5
98	20.12097166651035	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129253.0
3	22444.0
4	17100.0
5	14752.0
6	13791.0
7	14231.0
8	16198.0
9	18888.0
10	20858.0
11	23166.0
12	25881.0
13	30242.0
14	24942.0
15	24822.0
16	27757.0
17	31720.0
18	35723.0
19	35074.0
20	36254.0
21	39584.0
22	43212.0
23	48250.0
24	53778.0
25	60650.0
26	69341.0
27	80284.0
28	93186.0
29	108881.0
30	129589.0
31	157821.0
32	198611.0
33	257737.0
34	352863.0
35	500512.0
36	691042.0
37	737797.0
38	320810.0
39	13684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25840261551297	17.38474934704431	13.93231490993113	24.42453312751159
2	15.745311001247803	18.836312080847357	38.99467990692161	26.423697010983233
3	20.289051194412338	22.323711131189032	27.463272091747726	29.923965582650908
4	13.916639073873727	14.969274613509075	34.87789525126558	36.23619106135161
5	15.750387489136802	35.78174964903511	32.50939780137269	15.95846506045539
6	34.885194750229495	33.21911357052528	16.63163705917757	15.264054620067661
7	31.16058648098133	30.323382490021274	19.130653395334672	19.385377633662728
8	27.89818886289406	33.37335072091036	18.8144262608464	19.91403415534918
9	26.877187036307376	16.74586474948237	18.93933343524846	37.437614778961795
10-11	26.57356844616349	25.026695494371353	25.927102190673253	22.47263386879191
12-13	26.817698427464737	22.04489538296602	26.541026376762026	24.59637981280722
14-15	23.938521416187523	23.576097286260417	24.158489179322785	28.326892118229267
16-17	24.262733857865655	26.78136953400605	24.6662381293561	24.289658478772193
18-19	24.276698809943287	26.09754003006571	26.13716948525311	23.4885916747379
20-21	24.978261536164116	25.159962437705673	25.944838599585747	23.91693742654447
22-23	24.610799366782942	24.91380068188859	25.893420419500764	24.581979531827706
24-25	24.410189694266226	25.225100483084727	25.93880321696399	24.42590660568505
26-27	24.504498181564976	25.21748102053764	25.949681606435377	24.328339191462007
28-29	24.36797952604041	25.48125882791718	25.824993388229757	24.325768257812655
30-31	24.587429288572345	25.266911949020816	25.752312041651887	24.39334672075495
32-33	24.44379337982348	25.305603291279184	25.70047226280312	24.55013106609422
34-35	24.61756590716471	25.339724533057513	25.769530085999982	24.273179473777795
36-37	24.637737145058214	25.335561335844105	25.640772445947096	24.385929073150585
38-39	24.62631532333043	25.35303382778171	25.654696443647886	24.365954405239975
40-41	24.71376279483756	25.274721851357366	25.65320427236315	24.358311081441922
42-43	24.64850659096718	25.202738671048706	25.598127741323808	24.550626996660306
44-45	24.658155502130015	25.258914803167364	25.56711255417899	24.515817140523627
46-47	24.785585176857687	25.341052700638777	25.50640417259491	24.366957949908624
48-49	24.67659522045383	25.377685459359984	25.34655356598049	24.599165754205696
50-51	24.538136193526533	25.54258585906212	25.398934188674982	24.520343758736363
52-53	24.712472319022865	25.5666440844874	25.358462880361976	24.36242071612776
54-55	24.54212311567014	25.29040314973456	25.519294304936157	24.64817942965914
56-57	24.682957057475825	25.42283420948706	25.46331570349441	24.430893029542705
58-59	24.76204633811646	25.379359389137935	25.455799424904086	24.402794847841523
60-61	24.80113309709722	25.231304331581022	25.4969865550917	24.470576016230055
62-63	24.589954445561556	25.414772272294538	25.490722286975164	24.50455099516874
64-65	24.590840837082844	25.345770930936585	25.520156701360282	24.543231530620293
66-67	24.621075227276975	25.491851142371125	25.378279415600197	24.508794214751706
68-69	24.639743443882853	25.377700388034903	25.387097925791036	24.59545824229121
70-71	24.606850654713515	25.20687595357015	25.543186237599834	24.6430871541165
72-73	24.727795963611733	25.186850275054717	25.434520661945093	24.650833099388453
74-75	24.49422778602924	25.46234324430904	25.4217976871143	24.621631282547423
76-77	24.65393891028111	25.246337129552465	25.432160313142237	24.667563647024192
78-79	24.674639602157765	25.155618259209078	25.487779455011612	24.681962683621546
80-81	24.629088658803123	25.295755103229123	25.36614983488074	24.70900640308701
82-83	24.732227544513524	25.256113503289512	25.368201713877088	24.643457238319876
84-85	24.72083610122374	25.31057869102699	25.362486704959935	24.606098502789333
86-87	24.69257705073073	25.245769476231022	25.464362092658206	24.59729138038004
88-89	24.635575317546266	25.13520055693961	25.369681555732193	24.859542569781937
90-91	24.702980963379645	25.095309777751456	25.326015610702076	24.875693648166827
92-93	24.657255703367763	25.315577201329177	25.435534649546693	24.591632445756368
94-95	24.775650218479544	25.15429030792275	25.381198768766843	24.688860704830866
96-97	24.730592066846306	25.18808467930347	25.365587945956957	24.715735307893272
98	24.793463178489237	25.231537243740114	25.22207041718927	24.75292916058138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	706.0
1	519.0
2	428.5
3	560.0
4	520.0
5	484.5
6	827.0
7	1648.5
8	2356.5
9	2417.0
10	2019.5
11	1783.5
12	1903.0
13	2142.0
14	2538.0
15	2961.5
16	3207.5
17	3291.0
18	3322.5
19	3232.5
20	3096.0
21	2949.0
22	2758.5
23	2808.0
24	3002.0
25	3359.0
26	4129.0
27	5182.5
28	6615.5
29	8901.0
30	12151.0
31	16724.0
32	23209.5
33	31178.5
34	40797.5
35	52772.0
36	65272.0
37	77731.0
38	90034.0
39	102028.0
40	114404.0
41	127406.5
42	142254.0
43	157821.0
44	171367.0
45	183187.0
46	198269.5
47	214588.5
48	219530.5
49	215096.0
50	212812.0
51	210803.0
52	208539.5
53	208362.0
54	205347.0
55	197096.0
56	183900.0
57	169649.5
58	154402.0
59	137664.5
60	119247.0
61	98034.0
62	77445.5
63	57841.0
64	41410.5
65	30098.0
66	22458.5
67	16875.5
68	12534.5
69	9370.5
70	6992.0
71	5171.0
72	3807.0
73	2647.5
74	1985.5
75	1455.0
76	953.0
77	567.5
78	337.5
79	211.5
80	119.0
81	66.5
82	47.0
83	31.0
84	22.5
85	14.5
86	11.0
87	13.0
88	10.0
89	8.5
90	6.5
91	5.0
92	7.0
93	6.5
94	4.5
95	2.5
96	3.0
97	4.0
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4941018349257022
2	0.0527791099132706
3	0.06136179836521905
4	0.058397674002948194
5	0.07038689343840196
6	0.06903755324363686
7	0.04680662052660545
8	0.022695459669327595
9	0.013869447575700196
10-11	0.01704371508305742
12-13	0.012719190360490611
14-15	0.016534947468637796
16-17	0.015517412239798547
18-19	0.058043748705960636
20-21	0.08769605249420005
22-23	0.10487248956362781
24-25	0.07301921283474697
26-27	0.09001868725568095
28-29	0.09205375771335944
30-31	0.06205858879366332
32-33	0.03614462095485506
34-35	0.4160502467744133
36-37	0.6505699966907984
38-39	0.5964968474104171
40-41	0.5912322086177271
42-43	0.5620554919473146
44-45	0.5475003141087011
46-47	0.5363184867570002
48-49	0.5249596967568055
50-51	0.5405102894931967
52-53	0.6248440516660149
54-55	0.5947935819186645
56-57	0.5496128057250956
58-59	0.5251145390742377
60-61	0.5392715509537402
62-63	0.5531852391915638
64-65	0.5708925642064729
66-67	0.5992840091241941
68-69	0.5501215733395152
70-71	0.4977295692198248
72-73	0.48233381880086573
74-75	0.461242083133513
76-77	0.47688115719415103
78-79	0.4701897570479799
80-81	0.4667721658989437
82-83	0.4875099762693088
84-85	0.4525709133573177
86-87	0.45514793192600833
88-89	0.48346195568501354
90-91	0.4658209916632896
92-93	0.4600807657527726
94-95	0.4719704436984486
96-97	0.4665730829193882
98	0.4601913674080812
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4520728.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.0132872282542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62351536239214	39.0302456666028
2	14.154479443699216	15.007509686304926
3	4.994358348139698	7.94302060892277
4	2.3140405365118872	4.906995824797125
5	1.2896217436058157	3.418354395478855
6	0.7788780747788797	2.4774532256425443
7	0.5451216738303855	2.022908430838185
8	0.388829175876975	1.6490490226793157
9	0.28797778245743005	1.3739984007094301
>10	1.4919908686855556	14.183694799991917
>50	0.08968672086484124	3.239180425369248
>100	0.03952728314547436	3.5930670119025487
>500	0.0013433098430101291	0.4953104923359637
>1k	6.296761685931151E-4	0.6592120084243539
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4876	0.10785873425696038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.2120331061722803E-5	2.2120331061722803E-5
3	0.0	0.0	0.0	2.2120331061722803E-5	2.2120331061722803E-5
4	0.0	0.0	0.0	2.2120331061722803E-5	2.2120331061722803E-5
5	0.0	0.0	0.0	4.424066212344561E-5	2.2120331061722803E-5
6	0.0	0.0	0.0	4.424066212344561E-5	2.2120331061722803E-5
7	0.0	0.0	0.0	4.424066212344561E-5	2.2120331061722803E-5
8	0.0	0.0	0.0	4.424066212344561E-5	2.2120331061722803E-5
9	0.0	0.0	0.0	4.424066212344561E-5	4.424066212344561E-5
10-11	0.0	0.0	0.0	6.636099318516841E-5	4.424066212344561E-5
12-13	0.0	0.0	0.0	6.636099318516841E-5	1.1060165530861401E-4
14-15	1.1060165530861402E-5	0.0	0.0	6.636099318516841E-5	1.5484231743205962E-4
16-17	2.2120331061722803E-5	0.0	0.0	6.636099318516841E-5	1.7696264849378243E-4
18-19	2.2120331061722803E-5	0.0	0.0	6.636099318516841E-5	2.322634761480894E-4
20-21	3.3180496592584203E-5	0.0	0.0	7.742115871602981E-5	2.9862446933325785E-4
22-23	4.424066212344561E-5	0.0	0.0	8.848132424689121E-5	3.2074480039498063E-4
24-25	4.424066212344561E-5	1.1060165530861402E-5	0.0	1.5484231743205962E-4	3.31804965925842E-4
26-27	4.424066212344561E-5	2.2120331061722803E-5	0.0	2.1014314508636662E-4	3.871057935801491E-4
28-29	4.424066212344561E-5	2.2120331061722803E-5	0.0	4.3134645570359464E-4	4.2028629017273325E-4
30-31	4.424066212344561E-5	2.2120331061722803E-5	0.0	0.0013604003602959525	4.2028629017273325E-4
32-33	4.424066212344561E-5	2.2120331061722803E-5	0.0	0.0035060724732830645	4.7558711782704025E-4
34-35	4.424066212344561E-5	2.2120331061722803E-5	0.0	0.006879422960195791	5.087676144196245E-4
36-37	4.424066212344561E-5	2.2120331061722803E-5	0.0	0.011679534800589638	5.087676144196245E-4
38-39	5.5300827654307003E-5	2.2120331061722803E-5	0.0	0.018603198422908876	5.308879454813473E-4
40-41	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.026942563233178375	5.308879454813473E-4
42-43	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.03500542390517633	5.419481110122086E-4
44-45	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.04403051897835924	5.640684420739314E-4
46-47	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.054283292425467755	6.083091041973771E-4
48-49	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.06403835842368752	6.414896007899613E-4
50-51	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.07423583104314171	6.414896007899613E-4
52-53	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.08453284515237369	6.525497663208227E-4
54-55	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.09536074720708701	6.63609931851684E-4
56-57	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.10650939406219528	6.967904284442682E-4
58-59	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.11800090604876029	7.078505939751297E-4
60-61	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.1308085777334978	7.078505939751297E-4
62-63	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.14276461667235896	7.078505939751297E-4
64-65	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.1555612281915656	7.078505939751297E-4
66-67	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.16965187907788304	7.078505939751297E-4
68-69	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.18352132665358323	7.078505939751297E-4
70-71	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.1978884816781722	7.631514216294367E-4
72-73	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.21266486282740302	8.737530769380507E-4
74-75	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.22797213192211518	9.290539045923577E-4
76-77	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.2427263927402843	9.290539045923577E-4
78-79	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.2594714833540085	9.290539045923577E-4
80-81	1.1060165530861401E-4	2.2120331061722803E-5	0.0	0.27721198886551013	9.732945667158034E-4
82-83	1.1060165530861401E-4	2.2120331061722803E-5	0.0	0.2949524943770119	0.0010838962220244173
84-85	1.2166182083947541E-4	4.424066212344561E-5	0.0	0.31348933180673555	0.0010838962220244173
86	1.3272198637033681E-4	4.424066212344561E-5	0.0	0.3271154557407568	0.0010838962220244173
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12715	0.0	28.924128	1
GTACATG	15575	0.0	24.558556	1
TATCAAC	15200	0.0	23.825148	2
TCAACGC	15145	0.0	23.760332	4
TACATGG	15835	0.0	23.593462	2
CAACGCA	15425	0.0	23.329025	5
ATCAACG	15670	0.0	22.90577	3
AACGCAG	16150	0.0	22.276321	6
ACATGGG	16480	0.0	22.141548	3
GTATAGG	1020	0.0	21.660624	1
GTACCTG	4390	0.0	21.494099	1
TACCTGG	4650	0.0	20.1108	2
GGTATCA	9005	0.0	19.93475	1
GAGTACA	12010	0.0	19.929216	1
ACGCAGA	18205	0.0	19.581842	7
AGTACAT	11545	0.0	18.74132	2
CGCAGAG	19085	0.0	18.697147	8
AGAGTAC	13635	0.0	18.477161	10-11
CATGGGG	13005	0.0	18.399763	4
GTATATG	1340	0.0	18.205431	1
>>END_MODULE