FastQCFastQC Report
Mon 15 Aug 2016
SRR2921435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4520728
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.11829953051809355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132400.029.0329631
TCAACGC154300.024.7822534
CAACGCA156950.024.4405365
TATCAAC157250.024.2555792
ATCAACG161550.023.6690333
AACGCAG167350.023.0070346
GTACATG156500.022.7358971
TACATGG163250.021.8220122
GAGTACA123300.021.4791721
GTACCTG43400.021.2920671
ACATGGG165550.020.6583143
AGTACAT118300.020.5977332
ACGCAGA190750.020.0362327
GGTATCA95100.019.6341151
CGTATAC1951.0337853E-519.4870873
TACCTGG46450.019.1224122
CGCAGAG200650.018.9293428
AGAGTAC145750.018.8599410-11
CATGGGG127550.018.6953724
GTATATA10750.018.6100121
Produced by FastQC (version 0.11.5)