##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921435_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4520728
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95943529449239	33.0	31.0	34.0	30.0	34.0
2	32.0794748102518	34.0	31.0	34.0	30.0	34.0
3	32.20526273644422	34.0	31.0	34.0	30.0	34.0
4	35.73403597827607	37.0	35.0	37.0	35.0	37.0
5	35.536634586287875	37.0	35.0	37.0	33.0	37.0
6	35.57491337678356	37.0	35.0	37.0	33.0	37.0
7	35.5102067631585	37.0	35.0	37.0	33.0	37.0
8	35.46662263246097	37.0	35.0	37.0	33.0	37.0
9	37.09700340299173	39.0	37.0	39.0	34.0	39.0
10-11	37.00926322043706	39.0	37.0	39.0	33.5	39.0
12-13	36.8954521262947	39.0	37.0	39.0	33.0	39.0
14-15	38.19950326584568	40.0	38.0	41.0	33.0	41.0
16-17	38.10401234049029	40.0	38.0	41.0	33.0	41.0
18-19	38.00908736380512	40.0	38.0	41.0	33.0	41.0
20-21	37.89897899187918	40.0	38.0	41.0	33.0	41.0
22-23	37.86743927084311	40.0	38.0	41.0	33.0	41.0
24-25	37.925728112817225	40.0	38.0	41.0	33.0	41.0
26-27	37.81747253982102	40.0	38.0	41.0	33.0	41.0
28-29	37.68776024569494	40.0	38.0	41.0	32.5	41.0
30-31	37.55791987485202	40.0	38.0	41.0	32.0	41.0
32-33	37.4029002629665	40.0	37.0	41.0	32.0	41.0
34-35	37.26057904833027	40.0	37.0	41.0	31.5	41.0
36-37	37.16209612699548	40.0	37.0	41.0	31.0	41.0
38-39	37.13081455022288	40.0	37.0	41.0	31.0	41.0
40-41	36.97943163136557	40.0	37.0	41.0	31.0	41.0
42-43	36.74955847819201	40.0	36.0	41.0	30.5	41.0
44-45	36.55278353397948	39.0	36.0	41.0	30.0	41.0
46-47	36.28271021835421	39.0	35.5	41.0	30.0	41.0
48-49	35.95302869360864	39.0	35.0	40.0	29.0	41.0
50-51	35.68218348903097	39.0	35.0	40.0	28.0	41.0
52-53	35.38154341513137	38.0	35.0	40.0	28.0	41.0
54-55	35.06084595224486	38.0	34.0	40.0	27.0	41.0
56-57	34.67978099987435	38.0	34.0	40.0	26.0	41.0
58-59	34.28971594840477	37.0	33.5	40.0	26.0	41.0
60-61	33.862508649049445	37.0	33.0	39.5	25.0	41.0
62-63	33.42994889318712	36.0	33.0	39.0	23.5	40.0
64-65	32.91812524442966	35.5	32.0	39.0	22.5	40.0
66-67	32.386264004381594	35.0	31.5	38.0	21.5	40.0
68-69	31.878697745141935	35.0	31.0	37.5	20.0	39.5
70-71	31.323596553475458	34.5	30.5	37.0	18.0	39.0
72-73	30.770073868633546	34.0	30.0	36.0	16.5	39.0
74-75	30.250265775777706	34.0	29.5	36.0	12.5	38.5
76-77	28.95061503368484	32.5	28.0	34.5	11.0	36.0
78-79	29.882936553581636	34.0	29.5	35.0	11.0	37.0
80-81	29.909655259064472	34.0	30.0	35.0	9.0	36.5
82-83	29.57416294455229	34.0	29.5	35.0	7.0	36.0
84-85	29.083188260828788	34.0	29.0	35.0	2.0	36.0
86-87	28.568972298267006	33.5	29.0	35.0	2.0	35.0
88-89	28.02532379740608	33.0	27.5	35.0	2.0	35.0
90-91	27.5503233107588	33.0	26.5	35.0	2.0	35.0
92-93	27.11149078201564	33.0	26.0	34.0	2.0	35.0
94-95	26.613144055559193	32.0	25.0	34.0	2.0	35.0
96-97	25.843038444250574	32.0	24.0	34.0	2.0	35.0
98-99	24.821695974630636	32.0	18.5	34.0	2.0	35.0
100-101	21.18255455758453	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28948.0
3	14990.0
4	8921.0
5	6679.0
6	5847.0
7	6006.0
8	7031.0
9	8395.0
10	9888.0
11	11801.0
12	13511.0
13	16228.0
14	18196.0
15	20351.0
16	23527.0
17	27564.0
18	28206.0
19	24824.0
20	25531.0
21	26582.0
22	28340.0
23	31531.0
24	35928.0
25	42394.0
26	49964.0
27	60249.0
28	73136.0
29	90044.0
30	110494.0
31	137976.0
32	178013.0
33	238182.0
34	339786.0
35	515887.0
36	810406.0
37	1002513.0
38	430259.0
39	12600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63419489278034	17.818213693668902	14.284614206743198	24.262977206807555
2	15.873859254527147	19.12530459695872	38.683791637099155	26.317044511414977
3	20.308959502599624	22.550544048253464	27.75085206979904	29.389644379347867
4	14.166365638922535	15.247232315505407	34.91990448566754	35.66649755990452
5	15.798785232912941	35.75603965932724	32.60626336352186	15.838911744237953
6	34.88186163381688	33.40522612807276	16.482042959486375	15.23086927862398
7	31.20569040072502	30.336731924517007	19.185444956591923	19.272132718166045
8	28.012545621145097	33.40107767983954	18.786301499690655	19.800075199324713
9	26.74545603893549	16.789934196062127	19.01125160768091	37.45335815732147
10-11	26.533730377510807	25.12427004495199	25.96446839876414	22.37753117877306
12-13	26.86739155801009	22.001996259449076	26.62688170590732	24.50373047663351
14-15	23.90452175772328	23.530385024614493	24.335145324109806	28.22994789355242
16-17	24.218593892761195	26.774849755340025	24.838879350423497	24.167677001475283
18-19	24.23814033316261	26.094580810526697	26.275759292741164	23.391519563569528
20-21	24.868835280300424	25.096293667363796	26.23411663393449	23.800754418401297
22-23	24.536240482437808	24.859661115368556	26.137244535873133	24.466853866320506
24-25	24.384571120743782	25.18159910772341	26.127657022722804	24.306172748810003
26-27	24.356105216183284	25.19869799519846	26.204782557808432	24.24041423080982
28-29	24.305904413167873	25.45066749267791	26.066724376827068	24.176703717327154
30-31	24.512781046593464	25.216993702468077	25.960438045512074	24.309787205426385
32-33	24.331676728993216	25.251100986352814	25.966377111164007	24.450845173489967
34-35	24.571787453271448	25.27088650214766	26.03499101128343	24.122335033297464
36-37	24.50628609517451	25.370695388943254	25.905178569038533	24.2178399468437
38-39	24.530120007464586	25.301146977424054	25.960171995175674	24.208561019935683
40-41	24.58304428201644	25.23053766918974	25.88806465398082	24.298353394812995
42-43	24.527259066511068	25.236117457090945	25.857376439763186	24.3792470366348
44-45	24.502213594655824	25.19324361111661	25.81972911658752	24.484813677640044
46-47	24.6444886292671	25.324419371714836	25.766090593489345	24.26500140552872
48-49	24.555148530839865	25.378665711043418	25.623123870451114	24.4430618876656
50-51	24.47680251303804	25.521759569067065	25.60748159741617	24.393956320478715
52-53	24.62625123959849	25.575380528808346	25.463603751434817	24.33476448015835
54-55	24.535571817556193	25.30285524422975	25.612120093997227	24.54945284421683
56-57	24.616050459000736	25.421953505533914	25.62405142170885	24.337944613756505
58-59	24.758379689769953	25.378430556039444	25.53175934359396	24.331430410596642
60-61	24.736803567229433	25.302108203924227	25.607518046191082	24.35357018265526
62-63	24.518275424026918	25.429480318416747	25.645857604476348	24.406386653079988
64-65	24.56851369127922	25.356886443065797	25.602481434096003	24.47211843155898
66-67	24.59134637726251	25.50801219565628	25.45491400061872	24.445727426462486
68-69	24.63190953300137	25.380750995079882	25.473701847662415	24.513637624256333
70-71	24.600532321225398	25.25811340773855	25.622754110822015	24.518600160214042
72-73	24.716258382250423	25.211224800143118	25.50669114097736	24.565825676629093
74-75	24.461362602730784	25.531400458339466	25.446571140548212	24.560665798381535
76-77	24.59309362233297	25.254262053420913	25.54321952879906	24.60942479544706
78-79	24.567064361159478	25.18666729683263	25.660553577186164	24.58571476482173
80-81	24.56046115289995	25.34516544884785	25.528513157621056	24.565860240631142
82-83	24.61861982260893	25.361090349254983	25.465670363088357	24.55461946504773
84-85	24.624669571165985	25.40663654199975	25.47871803789655	24.489975848937718
86-87	24.54271673734531	25.399298244526136	25.543560407455345	24.514424610673206
88-89	24.563818599064486	25.24314584792312	25.474510522550354	24.718525030462033
90-91	24.592170998853657	25.24690791300283	25.43052662133492	24.73039446680859
92-93	24.605099200045856	25.482599976563392	25.504786191830757	24.407514631559994
94-95	24.62653697885627	25.311233932263427	25.470301925009192	24.591927163871112
96-97	24.520122544589874	25.35829736552138	25.585817602876865	24.53576248701188
98-99	24.62303664805641	25.33476451722874	25.45171557343847	24.59048326127638
100-101	25.01326768107924	24.73345731987754	25.65341255375927	24.59986244528395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	287.0
1	227.5
2	257.5
3	390.0
4	333.5
5	285.0
6	601.0
7	1391.5
8	2090.5
9	2063.5
10	1512.0
11	1082.5
12	997.5
13	1039.0
14	1108.0
15	1209.0
16	1340.5
17	1465.5
18	1591.5
19	1655.0
20	1731.5
21	1886.0
22	2064.0
23	2332.0
24	2647.0
25	3134.0
26	3750.0
27	4644.0
28	6008.0
29	7972.0
30	10874.0
31	14687.0
32	20097.0
33	26792.5
34	34644.5
35	44654.0
36	56237.0
37	68274.0
38	80193.5
39	91999.0
40	104007.5
41	115829.0
42	128563.5
43	143934.5
44	159925.5
45	174352.0
46	185310.0
47	197688.0
48	214880.5
49	219837.0
50	212607.0
51	207448.5
52	205096.0
53	203404.5
54	201172.5
55	195147.5
56	182509.0
57	166948.0
58	152074.0
59	136963.0
60	117892.0
61	97207.5
62	77650.0
63	58162.5
64	41366.0
65	29894.0
66	22290.5
67	16974.0
68	12729.0
69	9281.5
70	6912.0
71	5196.0
72	3796.5
73	2702.5
74	1961.0
75	1418.0
76	960.5
77	551.0
78	309.5
79	180.5
80	102.0
81	45.5
82	20.5
83	10.5
84	3.5
85	3.0
86	1.0
87	0.5
88	1.5
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32202777959656054
2	0.0
3	0.005861887731356543
4	0.06352959080926789
5	5.751286076047929E-4
6	0.0639277567683789
7	0.07445703435375894
8	0.10462916592194886
9	0.09133484695385345
10-11	0.09631192144274109
12-13	0.09469713727523532
14-15	0.06328626716758894
16-17	0.03537040936769476
18-19	0.0376819839636448
20-21	0.02698680389530182
22-23	0.04081201080887857
24-25	0.03321367708917679
26-27	0.020549787556340484
28-29	0.022463196193179505
30-31	0.003262748831604113
32-33	0.005220398130566581
34-35	0.007487732064393169
36-37	0.010473976757725746
38-39	0.01587133753678611
40-41	0.02020692242488378
42-43	0.003306989493727559
44-45	0.013084175823009038
46-47	0.0238125363879446
48-49	0.0026101990652832906
50-51	0.006702460311702008
52-53	0.002455356747851231
54-55	0.0037383359494311532
56-57	0.0032516886660732516
58-59	0.0011170767186170015
60-61	0.005419481110122086
62-63	0.0135818832718978
64-65	0.006138391869628077
66-67	0.03853361670952112
68-69	0.060808790088675985
70-71	0.011613173807404472
72-73	0.03166525391485619
74-75	0.04961590257144424
76-77	0.3098947780092056
78-79	0.07519806544432667
80-81	0.03198599871525117
82-83	0.027031044557425263
84-85	0.018669559416094047
86-87	0.11806726704194545
88-89	0.5341396341474205
90-91	0.13133946567897914
92-93	0.04770249393460522
94-95	0.007388190574615416
96-97	0.005552203096492424
98-99	0.010197472619454213
100-101	0.007631514216294367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4520728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.43592008195364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32935372653381	34.76796310240374
2	15.673108402192693	15.49629068813188
3	5.6940163810554445	8.444668162775754
4	2.6916938721200343	5.322654525888414
5	1.4665785523342165	3.6250830053550795
6	0.9023058950458552	2.6763793270177523
7	0.6179549803171639	2.138442111484318
8	0.4178806690722317	1.652665228803853
9	0.31973302862892794	1.4225666805774608
>10	1.7259064476926402	15.354506779707492
>50	0.11016990039339192	3.702663210156413
>100	0.04887512746547421	4.089228760275587
>500	0.0016602157752002417	0.5559978152844114
>1k	7.179307038464088E-4	0.6322600464605393
>5k	4.487066899037904E-5	0.11863055567729663
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5348	0.11829953051809355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2120331061722803E-5
2	0.0	0.0	0.0	0.0	2.2120331061722803E-5
3	0.0	0.0	0.0	0.0	2.2120331061722803E-5
4	0.0	0.0	0.0	0.0	2.2120331061722803E-5
5	0.0	0.0	0.0	0.0	2.2120331061722803E-5
6	0.0	0.0	0.0	0.0	2.2120331061722803E-5
7	0.0	0.0	0.0	4.424066212344561E-5	2.2120331061722803E-5
8	0.0	0.0	0.0	4.424066212344561E-5	2.2120331061722803E-5
9	0.0	0.0	0.0	6.636099318516841E-5	2.2120331061722803E-5
10-11	0.0	0.0	0.0	6.636099318516841E-5	2.2120331061722803E-5
12-13	0.0	0.0	0.0	6.636099318516841E-5	1.4378215190119823E-4
14-15	1.1060165530861402E-5	0.0	0.0	6.636099318516841E-5	1.9908297955550523E-4
16-17	2.2120331061722803E-5	0.0	0.0	6.636099318516841E-5	2.875643038023964E-4
18-19	2.2120331061722803E-5	0.0	0.0	6.636099318516841E-5	3.0968463486411924E-4
20-21	2.2120331061722803E-5	0.0	0.0	6.636099318516841E-5	4.0922612464187186E-4
22-23	2.2120331061722803E-5	0.0	0.0	8.848132424689121E-5	4.534667867653174E-4
24-25	2.2120331061722803E-5	0.0	0.0	1.3272198637033681E-4	4.8664728335790164E-4
26-27	4.424066212344561E-5	0.0	0.0	1.8802281402464382E-4	5.751286076047928E-4
28-29	6.636099318516841E-5	0.0	0.0	4.3134645570359464E-4	5.861887731356543E-4
30-31	6.636099318516841E-5	0.0	0.0	0.0013604003602959523	5.972489386665157E-4
32-33	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.0035060724732830645	6.414896007899613E-4
34-35	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.006813061967010623	6.414896007899613E-4
36-37	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.011535752648688441	6.414896007899613E-4
38-39	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.01847047643653854	6.414896007899613E-4
40-41	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.026787720915746313	6.414896007899613E-4
42-43	6.636099318516841E-5	2.2120331061722803E-5	0.0	0.034883762084336856	6.525497663208227E-4
44-45	7.742115871602981E-5	2.2120331061722803E-5	0.0	0.04376507500561856	6.746700973825454E-4
46-47	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.05386300613529502	7.18910759505991E-4
48-49	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.06351853064373703	7.963319182220208E-4
50-51	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.07357222111129004	8.184522492837437E-4
52-53	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.08375863356521339	8.848132424689121E-4
54-55	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.09446487379908722	9.622344011849419E-4
56-57	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.10554715966101035	9.843547322466648E-4
58-59	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.11676216750930382	0.0010064750633083876
60-61	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.12930439522130066	0.001017535228839249
62-63	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.14091756902870511	0.0010396555599009717
64-65	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.15317223243689954	0.0010396555599009717
66-67	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.16677623603985908	0.0010396555599009717
68-69	8.848132424689121E-5	2.2120331061722803E-5	0.0	0.18013691600113962	0.0010396555599009717
70-71	1.1060165530861401E-4	2.2120331061722803E-5	0.0	0.19391788225259293	0.0010396555599009717
72-73	1.1060165530861401E-4	4.424066212344561E-5	0.0	0.2084066990980214	0.0011170767186170015
74-75	1.1060165530861401E-4	4.424066212344561E-5	0.0	0.22309459892300532	0.001161317380740447
76-77	1.1060165530861401E-4	4.424066212344561E-5	0.0	0.2374506937820634	0.0011723775462713084
78-79	1.1060165530861401E-4	4.424066212344561E-5	0.0	0.25388609976092347	0.0012276783739256156
80-81	1.1060165530861401E-4	4.424066212344561E-5	0.0	0.2711510181545981	0.0012608588705181997
82-83	1.1060165530861401E-4	4.424066212344561E-5	0.0	0.28847123737592706	0.001459941850073705
84-85	1.1060165530861401E-4	6.636099318516841E-5	0.0	0.306510367356762	0.0014820621811354278
86-87	1.1060165530861401E-4	7.742115871602981E-5	0.0	0.3240517898887082	0.0015484231743205963
88-89	1.1060165530861401E-4	8.848132424689121E-5	0.0	0.3438052455268267	0.0016479646640983488
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13240	0.0	29.032963	1
TCAACGC	15430	0.0	24.782253	4
CAACGCA	15695	0.0	24.440536	5
TATCAAC	15725	0.0	24.255579	2
ATCAACG	16155	0.0	23.669033	3
AACGCAG	16735	0.0	23.007034	6
GTACATG	15650	0.0	22.735897	1
TACATGG	16325	0.0	21.822012	2
GAGTACA	12330	0.0	21.479172	1
GTACCTG	4340	0.0	21.292067	1
ACATGGG	16555	0.0	20.658314	3
AGTACAT	11830	0.0	20.597733	2
ACGCAGA	19075	0.0	20.036232	7
GGTATCA	9510	0.0	19.634115	1
CGTATAC	195	1.0337853E-5	19.487087	3
TACCTGG	4645	0.0	19.122412	2
CGCAGAG	20065	0.0	18.929342	8
AGAGTAC	14575	0.0	18.85994	10-11
CATGGGG	12755	0.0	18.695372	4
GTATATA	1075	0.0	18.610012	1
>>END_MODULE