FastQCFastQC Report
Mon 15 Aug 2016
SRR2921434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5428266
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81460.15006633794290847No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69280.1276282333990265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG183100.024.5123861
GTATCAA194100.024.427611
TACATGG191000.022.6327722
ACATGGG192600.021.6353053
TATCAAC225600.020.766882
TCAACGC226400.020.4503334
CAACGCA227450.020.3760815
ATCAACG231200.020.1248953
AACGCAG236200.019.7124736
GAGTACA142400.018.9110281
GTATAGG11750.018.804971
CATGGGG154200.018.7883284
GTATATA14450.018.4769131
GTACCTG50700.018.4313581
AGAGTAC173100.018.24586110-11
AGTACAT139750.018.2054162
GTGTAGC30850.017.7566531
ACGCAGA262550.017.6065527
CGCAGAG274350.016.8130958
TACCTGG51850.016.35622
Produced by FastQC (version 0.11.5)