##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921434_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5428266 Sequences flagged as poor quality 0 Sequence length 98 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.426807934614846 31.0 31.0 34.0 27.0 34.0 2 30.536866100519024 31.0 31.0 34.0 27.0 34.0 3 30.66775393836632 31.0 31.0 34.0 27.0 34.0 4 34.17477164899436 37.0 35.0 37.0 30.0 37.0 5 34.165087893629384 37.0 35.0 37.0 30.0 37.0 6 34.26212459006246 37.0 35.0 37.0 30.0 37.0 7 34.15392852892618 37.0 35.0 37.0 30.0 37.0 8 34.138853364960376 37.0 35.0 37.0 30.0 37.0 9 35.60126217101373 39.0 37.0 39.0 30.0 39.0 10-11 35.48051385101614 39.0 36.0 39.0 30.0 39.0 12-13 35.32402677024302 39.0 35.0 39.0 30.0 39.0 14-15 36.34625016533825 40.0 36.5 41.0 29.5 41.0 16-17 36.20418122472259 40.0 36.0 41.0 29.0 41.0 18-19 36.03287200737768 39.0 36.0 41.0 28.0 41.0 20-21 35.91942104532092 39.0 36.0 41.0 27.0 41.0 22-23 35.96284890976234 39.0 36.0 41.0 27.5 41.0 24-25 35.8285362213274 39.0 36.0 41.0 27.0 41.0 26-27 35.5643605158627 39.0 36.0 41.0 27.0 41.0 28-29 35.33687626951222 39.0 35.0 40.0 26.0 41.0 30-31 35.08797118637885 39.0 35.0 40.0 25.0 41.0 32-33 34.81404006362253 38.5 34.5 40.0 24.0 41.0 34-35 34.39059609459079 38.0 34.0 40.0 22.5 41.0 36-37 34.00736284846763 38.0 33.5 40.0 19.0 41.0 38-39 33.77685498463045 38.0 33.0 40.0 18.0 41.0 40-41 33.518216038049715 38.0 33.0 40.0 16.5 41.0 42-43 33.23029435182433 38.0 33.0 40.0 15.0 41.0 44-45 32.90869054685235 38.0 32.0 40.0 12.5 41.0 46-47 32.60791807181151 37.0 31.5 40.0 9.0 41.0 48-49 32.38573266306405 37.0 31.0 40.0 8.0 41.0 50-51 31.633395452617833 36.0 30.5 39.0 8.0 40.0 52-53 32.1506295012072 37.0 31.5 39.0 7.5 40.0 54-55 32.58522076847376 37.0 32.0 40.0 7.0 40.5 56-57 32.433092998758724 37.0 32.0 40.0 2.0 41.0 58-59 32.09621359749136 36.5 31.5 39.5 2.0 41.0 60-61 31.63517594753094 36.0 31.0 39.0 2.0 40.5 62-63 31.153391248697098 35.5 30.5 39.0 2.0 40.0 64-65 30.610361117159698 35.0 29.5 38.5 2.0 40.0 66-67 30.113932515466267 35.0 29.0 38.0 2.0 40.0 68-69 29.59003003905851 34.0 29.0 37.0 2.0 39.5 70-71 29.060219414450213 34.0 28.0 36.5 2.0 39.0 72-73 28.501806193727425 34.0 27.0 36.0 2.0 39.0 74-75 27.90399705909769 33.5 26.0 35.5 2.0 38.0 76-77 27.31328042877781 33.0 26.0 35.0 2.0 37.0 78-79 26.742072330280056 33.0 25.0 35.0 2.0 36.5 80-81 26.153836418480598 32.0 23.5 35.0 2.0 36.0 82-83 25.514837058463975 32.0 20.5 34.5 2.0 35.5 84-85 24.8912393018323 31.0 18.5 34.0 2.0 35.0 86-87 24.265938146730463 31.0 14.5 34.0 2.0 35.0 88-89 23.601907773126815 30.5 6.0 34.0 2.0 35.0 90-91 22.845882920991713 30.0 2.0 34.0 2.0 35.0 92-93 22.01245093737116 29.5 2.0 34.0 2.0 35.0 94-95 21.08366364139119 29.0 2.0 34.0 2.0 35.0 96-97 20.1726626882323 28.0 2.0 33.0 2.0 34.0 98 19.54098951672597 27.0 2.0 33.0 2.0 34.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 164709.0 3 28337.0 4 21727.0 5 18829.0 6 17405.0 7 18226.0 8 20823.0 9 23985.0 10 26850.0 11 29715.0 12 33013.0 13 37418.0 14 31914.0 15 31720.0 16 36002.0 17 41456.0 18 46252.0 19 45104.0 20 46965.0 21 50855.0 22 55595.0 23 61546.0 24 69352.0 25 77781.0 26 88098.0 27 100332.0 28 116615.0 29 136416.0 30 162784.0 31 197406.0 32 245821.0 33 317842.0 34 427379.0 35 600520.0 36 805540.0 37 832033.0 38 347341.0 39 14560.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2911243940077 17.354341992107404 14.017531078367753 24.337002535517144 2 15.786785516470411 18.88626232297176 38.888750648577464 26.438201511980363 3 20.385617954827946 22.20834178970573 27.575645131350733 29.830395124115583 4 14.011238720035687 14.97855758392404 34.744455985673724 36.265747710366554 5 15.78582541178735 35.754241851683 32.510585156796004 15.949347579733649 6 35.044389003893315 33.14955362121986 16.4110729442675 15.394984430619333 7 31.12299739239866 30.352181000118694 19.139403090048134 19.38541851743451 8 27.883725601115906 33.16634082613014 18.86520889111644 20.08472468163752 9 26.919120555735603 16.702026891704485 19.00195542197999 37.37689713057992 10-11 26.673208261793274 24.973456054419486 25.768701148200112 22.584634535587128 12-13 26.97263346949334 22.042941759930635 26.395468092893665 24.588956677682358 14-15 23.994135941284974 23.60943261095436 24.224526785791607 28.171904661969055 16-17 24.298369482904942 26.724596018910123 24.72113503476277 24.255899463422164 18-19 24.312496376781876 26.053972839665697 26.18705842245106 23.44647236110137 20-21 24.94628372628772 25.13402562929585 26.044994259139507 23.87469638527692 22-23 24.68826037708987 24.790455426803337 26.007842545671956 24.51344165043484 24-25 24.499533065601543 25.04481989495428 26.059253426697477 24.396393612746706 26-27 24.481541264834643 25.133221814228495 26.140461705320355 24.24477521561651 28-29 24.406937364372787 25.44723039520975 25.930295369869004 24.21553687054845 30-31 24.58220130892545 25.17609993969398 25.80150185309903 24.440196898281542 32-33 24.461312068762165 25.237881111045585 25.77471693106092 24.526089889131335 34-35 24.738189929602772 25.17541031903759 25.868125332133296 24.218274419226336 36-37 24.669621863773195 25.19486489611362 25.847448001644462 24.288065238468718 38-39 24.616496486823422 25.251947623028364 25.85134170513077 24.280214185017446 40-41 24.722918913920164 25.214918591084757 25.726497809417214 24.335664685577864 42-43 24.639606244746957 25.169975643193204 25.64223442321853 24.548183688841306 44-45 24.674984140880298 25.12828312027451 25.645944793684176 24.55078794516102 46-47 24.848811794995278 25.2354090648095 25.604817648039415 24.31096149215581 48-49 24.772901426944944 25.284608820583866 25.462366925658014 24.480122826813176 50-51 24.557038204959905 25.460183752123104 25.575101764215617 24.40767627870137 52-53 24.706477562040106 25.516536643795174 25.387672456192945 24.389313337971778 54-55 24.632145362108407 25.217999516838514 25.53830945186747 24.61154566918561 56-57 24.759092527901753 25.317095151933085 25.47595417531734 24.447858144847824 58-59 24.858067983648603 25.255833223444906 25.483265227202352 24.402833565704142 60-61 24.80669829782623 25.18852276063523 25.612375976742484 24.392402964796055 62-63 24.546297870093735 25.355337131339667 25.60726342080851 24.49110157775809 64-65 24.61804195157626 25.29898078742655 25.592924017138905 24.490053243858277 66-67 24.673093015562056 25.30422139210582 25.43159617421571 24.59108941811641 68-69 24.606182063285598 25.31547996330921 25.473563816942846 24.60477415646235 70-71 24.730007182033916 25.136998361592727 25.527131480100728 24.605862976272626 72-73 24.748862327460866 25.134750930149814 25.49277854233519 24.623608200054132 74-75 24.4878359090801 25.529029934952906 25.397295893905003 24.585838262061994 76-77 24.708520092211767 25.178510589479465 25.410159935973407 24.70280938233536 78-79 24.67626015134514 25.15431784552519 25.511588553862058 24.657833449267617 80-81 24.698690088889304 25.267858539336064 25.42489350303006 24.608557868744573 82-83 24.78537412544787 25.226164228215588 25.344259949064625 24.64420169727192 84-85 24.702647618076465 25.3134392385499 25.361732194968074 24.622180948405557 86-87 24.676258087417303 25.24589135419256 25.45812272905026 24.61972782933988 88-89 24.654533721068752 25.148554827206926 25.351163460802418 24.845747990921904 90-91 24.736356920039132 25.052124289812628 25.371695305218502 24.83982348492974 92-93 24.7604043635311 25.25077921850776 25.401491520605322 24.587324897355817 94-95 24.792875645978 25.10843156721665 25.39723997086094 24.701452815944407 96-97 24.719038316810096 25.08208867650102 25.47256054109302 24.726312465595868 98 24.840107466142907 25.16029971475128 25.236831214635412 24.762761604470402 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 904.0 1 697.5 2 646.5 3 884.5 4 817.5 5 794.5 6 1449.5 7 3139.5 8 4533.5 9 4253.5 10 3224.0 11 2587.0 12 2592.5 13 3008.5 14 3517.0 15 3911.5 16 4215.5 17 4402.5 18 4475.5 19 4332.0 20 4125.5 21 3897.0 22 3744.5 23 3902.0 24 4244.5 25 4822.0 26 5911.5 27 7716.0 28 10197.5 29 13742.0 30 18392.0 31 24204.0 32 31419.5 33 39914.5 34 50139.5 35 61755.0 36 74454.5 37 87938.5 38 101570.0 39 115828.0 40 130999.0 41 147310.5 42 164865.5 43 182990.0 44 199614.0 45 215121.5 46 235090.5 47 255872.0 48 262816.5 49 257957.0 50 254946.0 51 253163.0 52 251671.5 53 252097.0 54 248137.0 55 237032.0 56 220197.0 57 201984.0 58 183257.0 59 164033.0 60 143199.5 61 119051.0 62 95788.5 63 73432.0 64 54077.0 65 40340.5 66 30506.0 67 23186.0 68 17135.5 69 12584.5 70 9391.0 71 6929.0 72 5123.5 73 3709.5 74 2720.5 75 1883.5 76 1214.5 77 740.0 78 428.5 79 248.5 80 145.5 81 85.5 82 56.0 83 40.5 84 29.5 85 20.0 86 19.0 87 19.0 88 16.0 89 14.0 90 14.5 91 10.5 92 7.0 93 9.5 94 9.0 95 7.0 96 5.0 97 4.5 98 3.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49741482823428324 2 0.05399514320042533 3 0.06195348569874799 4 0.06025865350003113 5 0.07230669978221406 6 0.07068555593996315 7 0.04771321081170304 8 0.023506585712638254 9 0.014608716669374714 10-11 0.017418085259639084 12-13 0.012996783871682042 14-15 0.017814160175643565 16-17 0.016727256917770793 18-19 0.05868356488057144 20-21 0.08958661937347949 22-23 0.10670074016269653 24-25 0.07472920450103218 26-27 0.09174200380010854 28-29 0.09363026793454854 30-31 0.06327066507057687 32-33 0.037323152550004 34-35 0.42128554496039805 36-37 0.6561856032847322 38-39 0.6014904206978803 40-41 0.5955400859132548 42-43 0.5674279779214946 44-45 0.5517876242615966 46-47 0.5409830689947766 48-49 0.5304364229755874 50-51 0.5459293999225535 52-53 0.6303117791206253 54-55 0.5997219001426974 56-57 0.5542009179358565 58-59 0.5314772710106689 60-61 0.5449438181548215 62-63 0.5574247835312418 64-65 0.5767127108362045 66-67 0.6037102824364171 68-69 0.5560062826692723 70-71 0.5027203898998318 72-73 0.48722741295286565 74-75 0.4667144167216566 76-77 0.48147051010396325 78-79 0.4749214574230519 80-81 0.47248053061511724 82-83 0.4921553217915261 84-85 0.4566191118858214 86-87 0.4599074547931144 88-89 0.4876787541362196 90-91 0.4707672763272839 92-93 0.4652774937705706 94-95 0.47704920871600615 96-97 0.4710896628868224 98 0.46537881526071123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 5428266.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.980831758775714 #Duplication Level Percentage of deduplicated Percentage of total 1 71.7126364297515 37.276824892331625 2 14.728641549958551 15.312140768874183 3 5.673393643927714 8.847231615189418 4 2.595684019337725 5.397032572325481 5 1.4263566322190586 3.707160206369641 6 0.8903148066039833 2.7767582504657153 7 0.5872425584128607 2.136774964131635 8 0.4106816426910045 1.7078058700110996 9 0.3103128218675414 1.4517286727478846 >10 1.5424945198404283 14.239044559791028 >50 0.08847236811738432 3.128104029328153 >100 0.032096615200844086 2.7279163954688443 >500 9.963221799778974E-4 0.345269919090688 >1k 6.049046396620181E-4 0.6673963958809094 >5k 7.116525172450244E-5 0.27881088799364734 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8146 0.15006633794290847 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6928 0.1276282333990265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8422089116487658E-5 2 0.0 1.8422089116487658E-5 0.0 0.0 1.8422089116487658E-5 3 0.0 1.8422089116487658E-5 0.0 0.0 1.8422089116487658E-5 4 0.0 1.8422089116487658E-5 0.0 9.21104455824383E-5 1.8422089116487658E-5 5 0.0 3.6844178232975316E-5 0.0 9.21104455824383E-5 1.8422089116487658E-5 6 0.0 3.6844178232975316E-5 0.0 9.21104455824383E-5 5.526626734946298E-5 7 0.0 3.6844178232975316E-5 0.0 1.1053253469892596E-4 5.526626734946298E-5 8 0.0 3.6844178232975316E-5 0.0 1.1053253469892596E-4 5.526626734946298E-5 9 0.0 3.6844178232975316E-5 0.0 1.1053253469892596E-4 5.526626734946298E-5 10-11 0.0 3.6844178232975316E-5 0.0 1.4737671293190127E-4 5.526626734946298E-5 12-13 0.0 3.6844178232975316E-5 0.0 1.4737671293190127E-4 1.1053253469892594E-4 14-15 0.0 3.6844178232975316E-5 0.0 1.4737671293190127E-4 1.565877574901451E-4 16-17 0.0 3.6844178232975316E-5 0.0 1.7500984660663276E-4 1.6579880204838893E-4 18-19 0.0 5.526626734946298E-5 0.0 2.210650693978519E-4 1.6579880204838893E-4 20-21 1.8422089116487658E-5 7.368835646595063E-5 0.0 2.3027611395609574E-4 2.486982030725834E-4 22-23 3.6844178232975316E-5 7.368835646595063E-5 0.0 2.6712029218907104E-4 3.0396447042204636E-4 24-25 3.6844178232975316E-5 7.368835646595063E-5 0.0 3.408086486550217E-4 3.500196932132655E-4 26-27 3.6844178232975316E-5 7.368835646595063E-5 0.0 3.8686387144624084E-4 3.77652826887997E-4 28-29 3.6844178232975316E-5 7.368835646595063E-5 0.0 8.10571921125457E-4 3.8686387144624084E-4 30-31 3.6844178232975316E-5 7.368835646595063E-5 0.0 0.002017218758255399 3.8686387144624084E-4 32-33 3.6844178232975316E-5 7.368835646595063E-5 0.0 0.0049739640614516675 4.052859605627285E-4 34-35 3.6844178232975316E-5 7.368835646595063E-5 0.0 0.008529427260933786 4.052859605627285E-4 36-37 3.6844178232975316E-5 7.368835646595063E-5 0.0 0.014212641753370229 4.052859605627285E-4 38-39 3.6844178232975316E-5 7.368835646595063E-5 0.0 0.023285520643240402 4.421301387957038E-4 40-41 3.6844178232975316E-5 8.289940102419446E-5 0.0 0.03311370518688657 4.7897431702867915E-4 42-43 3.6844178232975316E-5 1.1053253469892596E-4 0.0 0.04213131780940728 4.88185361586923E-4 44-45 3.6844178232975316E-5 1.1053253469892596E-4 0.0 0.052733230095945924 5.158184952616544E-4 46-47 3.6844178232975316E-5 1.1053253469892596E-4 0.0 0.06452336713049803 5.710847626111174E-4 48-49 4.605522279121915E-5 1.1053253469892596E-4 0.0 0.07566873104597306 5.895068517276051E-4 50-51 5.526626734946298E-5 1.1053253469892596E-4 0.0 0.08701673794172946 5.895068517276051E-4 52-53 5.526626734946298E-5 1.1053253469892596E-4 0.0 0.09747127351533622 6.171399854023366E-4 54-55 6.44773119077068E-5 1.1053253469892596E-4 0.0 0.10985091740161591 6.263510299605804E-4 56-57 7.368835646595063E-5 1.1053253469892596E-4 0.0 0.12297665589711337 6.355620745188242E-4 58-59 7.368835646595063E-5 1.289546238154136E-4 0.0 0.13600107290247015 6.44773119077068E-4 60-61 7.368835646595063E-5 1.4737671293190127E-4 0.0 0.14888732423945328 6.631952081935557E-4 62-63 7.368835646595063E-5 1.4737671293190127E-4 0.0 0.16155251050703853 7.092504309847748E-4 64-65 7.368835646595063E-5 1.4737671293190127E-4 0.0 0.17524933376514712 7.184614755430187E-4 66-67 7.368835646595063E-5 1.6579880204838893E-4 0.0 0.1902541253505263 7.368835646595064E-4 68-69 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.20565499185191 7.55305653775994E-4 70-71 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.22079794910566283 7.921498320089693E-4 72-73 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.23686201081524008 8.474160993584323E-4 74-75 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.2523826208958809 8.842602775914076E-4 76-77 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.26873222498676375 9.118934112661392E-4 78-79 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.2866845508307809 9.395265449408706E-4 80-81 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.3045816104074487 9.76370723173846E-4 82-83 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.32271815714263086 0.001086903257872772 84-85 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.341803441467312 0.001086903257872772 86 9.21104455824383E-5 1.6579880204838893E-4 0.0 0.3571858858795792 0.001086903257872772 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18310 0.0 24.512386 1 GTATCAA 19410 0.0 24.42761 1 TACATGG 19100 0.0 22.632772 2 ACATGGG 19260 0.0 21.635305 3 TATCAAC 22560 0.0 20.76688 2 TCAACGC 22640 0.0 20.450333 4 CAACGCA 22745 0.0 20.376081 5 ATCAACG 23120 0.0 20.124895 3 AACGCAG 23620 0.0 19.712473 6 GAGTACA 14240 0.0 18.911028 1 GTATAGG 1175 0.0 18.80497 1 CATGGGG 15420 0.0 18.788328 4 GTATATA 1445 0.0 18.476913 1 GTACCTG 5070 0.0 18.431358 1 AGAGTAC 17310 0.0 18.245861 10-11 AGTACAT 13975 0.0 18.205416 2 GTGTAGC 3085 0.0 17.756653 1 ACGCAGA 26255 0.0 17.606552 7 CGCAGAG 27435 0.0 16.813095 8 TACCTGG 5185 0.0 16.3562 2 >>END_MODULE